BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0164.0016.p (394 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3275 [D] COG0772 Bacterial cell division membrane protein 165 1e-40 SP0803 [D] COG0772 Bacterial cell division membrane protein 137 2e-32 lin2521 [D] COG0772 Bacterial cell division membrane protein 126 6e-29 L115706 [D] COG0772 Bacterial cell division membrane protein 123 5e-28 FN1027 [D] COG0772 Bacterial cell division membrane protein 117 3e-26 >BH3275 [D] COG0772 Bacterial cell division membrane protein Length = 398 Score = 165 bits (417), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 103/342 (30%), Positives = 173/342 (50%), Gaps = 12/342 (3%) Query: 43 RMIKFYVLGIIAFFGLTFVDYRLLVKYALYIYVTGIGILVLVSFIGKEQNGAQGWIKFGG 102 R + FYV+G I G+ DY LL +++ YV G+ +L+ V G +NG+Q W+ F G Sbjct: 52 RQVIFYVVGFIVMIGIMSFDYELLKNFSIPFYVIGLLMLIYVELNGVVRNGSQRWMNFFG 111 Query: 103 F--SLQPAEXXXXXXXXXXXXXXXRKHKNKL--QFWQDVV---PXXXXXXXXXXIVISQN 155 F QP+E N+ D+V +++ Q Sbjct: 112 FGPEFQPSEFMKFFLIIALAHMLYLLTTNRTDKSLKGDLVLLGKILAVGMPPFLLILKQP 171 Query: 156 DLGNALSYIVILVGLLWIGNIKFTHXXXXXXXXXXXXXXXXXSYIH-YHDEIKGFLTDIG 214 DLG AL ++ ++ + I + Y+H +H E F +++ Sbjct: 172 DLGTALVIGSVIATMILVAGISWR--LIFLCLLAVVGGIVTLVYLHNFHYEF--FSSNLI 227 Query: 215 RSHWVERFDPWLVPDEATAKASYHTKNAKLAIAXXXXXXXXXXXXXXVQTDRVPYTYSDS 274 ++H ++R WL PDE +Y T A L I Q+ VP ++D Sbjct: 228 KAHQLDRIYGWLNPDEYAGSFAYQTTQAILGIGAGQLFGSGFMNSVQAQSAAVPELHTDF 287 Query: 275 IFVQIAEEFGFVGSALVLLLYFILIHRMILIALESKDRGGPFLIVGIVAMMLYQIFENIG 334 IF I EEFGF+G+ ++L+++F++ +RM++IAL + G +L+ GI+ ++++Q+ +NIG Sbjct: 288 IFAVIGEEFGFIGATVLLVVFFLMFYRMVIIALTCNNLFGTYLVSGIIGLLVFQVVQNIG 347 Query: 335 AFIGLMPLTGITLPFISYGGTSLMINMASIGLVMSVRLHGQE 376 +GL P+TG+ LPFISYGGT+L+ NM +IG+V++V + + Sbjct: 348 MTVGLFPVTGLALPFISYGGTALVTNMIAIGIVLNVGMRTKN 389 >SP0803 [D] COG0772 Bacterial cell division membrane protein Length = 407 Score = 137 bits (346), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 99/355 (27%), Positives = 167/355 (47%), Gaps = 27/355 (7%) Query: 43 RMIKFYVLGIIAFFGLTFVDYRLLVKYALYIYVTGIGILVL-VSFIGKE---QNGAQGWI 98 + + + LG++ F + + L K ++Y+ G+G+++L + F GA+ W+ Sbjct: 46 QQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAKNWV 105 Query: 99 KFGGFSL-QPAEXXXXX---XXXXXXXXXXRKHKNKLQFWQDVVP--------XXXXXXX 146 G +L QP+E +KHK W+ VP Sbjct: 106 SINGITLFQPSEFMKISYILMLARVIVQFTKKHKE----WRRTVPLDFLLIFWMILFTIP 161 Query: 147 XXXIVISQNDLGNALSYIVILVGLLWIGNIKFTHXXXXXXXXXXXXXXXXXSYIHYHDEI 206 ++ Q+DLG AL ++ I G++ + + + +I + Sbjct: 162 VLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFIS--KDG 219 Query: 207 KGFLTDIGR-SHWVERFDPWLVPDEATAKASYHTKNAKLAIAXXXXXXXXXXXXXXVQTD 265 + FL IG ++ + R WL P E +Y ++AI + Sbjct: 220 RAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL--- 276 Query: 266 RVPYTYSDSIFVQIAEEFGFVGSALVLLLYFILIHRMILIALESKDRGGPFLIVGIVAMM 325 +P SD IF IAE+FGF+GS LV+ LY +LI+RM+ I L+S ++ ++ G++ M+ Sbjct: 277 -IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMML 335 Query: 326 LYQIFENIGAFIGLMPLTGITLPFISYGGTSLMINMASIGLVMSVRLHGQEVEEE 380 L+ IFENIGA GL+PLTGI LPFIS GG++++ N+ +GL++S+ EE+ Sbjct: 336 LFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEK 390 >lin2521 [D] COG0772 Bacterial cell division membrane protein Length = 331 Score = 126 bits (316), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 0/372 (0%), Positives = 0/372 (0%), Gaps = 30/372 (8%) Query: 26 SIYSVTHGRDKLDGAHL-RMIKFYVLGIIAFFGLTFVDYRLLVKYALYIYVTGIGILVLV 84 + L + + + + L+ Sbjct: 29 LSSQQRTSLESLMTKVTSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLL 88 Query: 85 SFIGKEQNGAQGWI--KFGGFSLQPAEXXXXXXXXXXXXX------XXRKHKNKLQFWQD 136 + ++ + + + Sbjct: 89 AAQKSHSARQLLAQLGEYLR-LGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQE- 146 Query: 137 VVPXXXXXXXXXXIVISQNDLGNALSYIVILVGLLWIGNIKFTHXXXXXXXXXXXXXXXX 196 + ++ G Sbjct: 147 GKMVIPQPQQREATDRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELI 206 Query: 197 XSYIHYHDEIKGFLTDIG-RSHWVERFDPWLVPDEATAKASYHTKNAKLAIAXXXXXXXX 255 + + H ++ + + ++ A L+ Sbjct: 207 PAKLFNHL----VTWLQARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLS 262 Query: 256 XXXXXXVQTDRVPYTYSDSIFVQIAEEFGFVGSALVLLLYFILIHRMILIALESKDRGGP 315 + Q + LL F + + R Sbjct: 263 AQS----SLSSAQAEDILNQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMA---TRPAL 315 Query: 316 F---LIVGIVAMMLYQIFENIGAFIGLMPLTGITLPFISYGGTSLMINMASIGLVMSVRL 372 + Sbjct: 316 WILL 375 Query: 373 HGQ----EVEEE 380 Sbjct: 376 387 >L115706 [D] COG0772 Bacterial cell division membrane protein Length = 414 Score = 123 bits (309), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 98/337 (29%), Positives = 156/337 (46%), Gaps = 19/337 (5%) Query: 61 VDYRLLVKYALYIYVTGIGILVLVSFIGKE----QNGAQGWIKFGGFSL-QPAEXXXXXX 115 +D R L + Y+ G+ ++VL F + GA+ W+ FGG +L QP+E Sbjct: 70 MDSRFLWNLTPFFYLLGLILMVLPIFFYDKATYASTGAKNWLAFGGRNLFQPSEFMKLSY 129 Query: 116 XXXXXXXXXRKHKN-KLQFWQD----VVPXXXXXXXXXXIVISQNDLGNALSYIVILVGL 170 N K + +D + + + Q D G L + I G+ Sbjct: 130 ILFSARIVVTFQNNLKKRVLKDDFRLIGLLILETIPVAILSVFQKDFGTFLVFAAIFAGI 189 Query: 171 LWIGNIKFTHXXXXXXXXXXXXXXXXXSYIHYHDEIKGFLTDIGRSHW-VERFDPWLVPD 229 + + I + + E + FL + + V RF WL P Sbjct: 190 ILVAGISWK--ILAPAFLFVAAVAVGIVALVASPEGQKFLESTSFAQYQVNRFIAWLHPF 247 Query: 230 EATAKASYHTKNAKLAIAXXXXXXXXXXXXXXVQTDRVPYTYSDSIFVQIAEEFGFVGSA 289 E + S + +++ V VP SD IF IAE+FGF+GSA Sbjct: 248 EYSQTFSLQQARSLISVGVGGLWGKGIG----VANVNVPVRESDMIFTVIAEDFGFIGSA 303 Query: 290 LVLLLYFILIHRMILIALESKDRGGPFLIVGIVAMMLYQIFENIGAFIGLMPLTGITLPF 349 ++ LYF+LI+RMI + +S ++ ++ GI M+L+ +FENIGA IG++PLTGI LPF Sbjct: 304 FLIFLYFMLIYRMIRVTFKSNNQFYTYISTGITMMILFHVFENIGAAIGVVPLTGIPLPF 363 Query: 350 ISYGGTSLMINMASIGLVMSVRLHG--QEVEEELPSP 384 IS GG++LM N+ +GLV+S++ + + V+E+ P Sbjct: 364 ISQGGSALMANIIGLGLVLSMKYNQLPEFVKEQRQIP 400 >FN1027 [D] COG0772 Bacterial cell division membrane protein Length = 366 Score = 117 bits (293), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 93/348 (26%), Positives = 167/348 (47%), Gaps = 16/348 (4%) Query: 26 SIYSVTHGRDKLDGAHLRMIKFYVLGIIAFFGLTFVDYRLLVKYALYIYVTGIGILVLVS 85 +IYS T + + L+ I ++++ I F G++ VDYR KYA IY+ I +L+ V Sbjct: 31 TIYSATITKS--EPFFLKEIVWFIISIFVFIGVSLVDYRKYYKYATAIYIFNILMLLSVL 88 Query: 86 FIGKEQNGAQGWIKFGGFSLQPAEXXXXXXXXXXXXXXXRKHKNKLQFWQDVVPXXXXXX 145 IG + GA+ WI G +LQP+E + ++ ++ + Sbjct: 89 VIGTSRLGAKRWIDLGPLALQPSEFSKLFLIFTFSAYLINNYSDRYTGFRAMFMSFLHIF 148 Query: 146 XXXXIVISQNDLGNALSYIVILVGLLWIGNIKFTHXXXXXXXXXXXXXXXXXSYIHYHDE 205 ++ + DLG +L I+I LL++ +++ I Y Sbjct: 149 PVFFLIAIEPDLGTSLVIILIYGMLLFLNKLEWKCIATVFFTIAAFIP------ISYKFL 202 Query: 206 IKGFLTDIGRSHWVERFDPWLVPDEATAKASYHTKNAKLAIAXXXXXXXXXXXXXXVQTD 265 +KG+ D R D +L P+ ++ +K+AI + Sbjct: 203 LKGYQKD--------RIDTFLNPELDALGTGWNITQSKIAIGSGKIFGKGFLNNTQGKLK 254 Query: 266 RVPYTYSDSIFVQIAEEFGFVGSALVLLLYFILIHRMILIALESKDRGGPFLIVGIVAMM 325 +P +++D I EE GF+G +++LL+Y +L+ ++I IA ++D+ G ++ GI + Sbjct: 255 YLPESHTDFIGSVFLEERGFLGGSMLLLIYIVLLVQIIYIADTTEDKFGRYICYGIATIF 314 Query: 326 LYQIFENIGAFIGLMPLTGITLPFISYGGTSLMINMASIGLVMSVRLH 373 + IF N+G +G+MP+TG+ L +SYGG+SL+ + +G+V SVR+H Sbjct: 315 FFHIFVNMGMIMGIMPVTGLPLLLMSYGGSSLVFSFLILGVVQSVRIH 362 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.144 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,788,939 Number of extensions: 884921 Number of successful extensions: 1968 Number of sequences better than 1.0e-05: 83 Number of HSP's gapped: 1926 Number of HSP's successfully gapped: 90 Length of query: 394 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 286 Effective length of database: 25,922,359 Effective search space: 7413794674 Effective search space used: 7413794674 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)