BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0164.0015.p (299 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll3434 [J] COG2890 Methylase of polypeptide chain release factors 152 6e-37 NMB2065 [J] COG2890 Methylase of polypeptide chain release factors 129 4e-30 NMA0369 [J] COG2890 Methylase of polypeptide chain release factors 129 6e-30 alr0115 [J] COG2890 Methylase of polypeptide chain release factors 121 1e-27 SMc02435 [J] COG2890 Methylase of polypeptide chain release factors 119 4e-27 >mll3434 [J] COG2890 Methylase of polypeptide chain release factors Length = 290 Score = 152 bits (384), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 112/286 (39%), Positives = 152/286 (53%), Gaps = 23/286 (8%) Query: 13 EASSFLDKSGCNEPQRSSQLLLEHVLGLSGAAYYMALADP---FPPEARSAWEAGVTRRA 69 EA + L +G +P ++L++EH SG A+ADP A +A +A + RRA Sbjct: 15 EARARLVAAGVGDPALDARLIVEH---FSGTTRTQAIADPERTIDSNAIAAIDAALGRRA 71 Query: 70 AXXXXPVQYIIGEQEFYGRVFEVTPDVLIPRPETELLVEAILKYGAELWPDGLVAAPGGA 129 PV I+G +EFYG ++P+ L PRP+TE LVEA+L + + AA G Sbjct: 72 GGE--PVHRILGYREFYGLRLSLSPETLEPRPDTETLVEAVLPFVKAM------AAREGT 123 Query: 130 ARPLTAVDIGAGSGAISVTLAAEAPAWRVCAGDISPGALAVAGRNAQRHGTAVDLRL--G 187 R L D+G G+GAI++ L + PA DIS GALA A RNA G Sbjct: 124 CRIL---DLGTGTGAIALALLSAVPAATATGVDISAGALATAARNAGELGLGGRFTTVQS 180 Query: 188 DLLEPFAGMETDILVSNPPYIPGGDIAGLQREVRDHEPRTALDGGDDGLDPYRRM-MEQL 246 D E +G ++ +NPPYIP DI LQ EVRD +PR ALDGG DGL+PYR + E Sbjct: 181 DWFEKVSG-RYHVIAANPPYIPTRDIGNLQDEVRDFDPRLALDGGVDGLNPYRIIAAEAA 239 Query: 247 PLLPAPPRLVGFELGQGQAGQVAKLLRAAGHWSEIVTIDDLAGIPR 292 L A R + E+G Q +V + +AAG+ S + + DL G R Sbjct: 240 RFLEAESR-IAVEIGHTQRDEVTDIFKAAGYAS-VAALRDLGGNDR 283 >NMB2065 [J] COG2890 Methylase of polypeptide chain release factors Length = 423 Score = 129 bits (325), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 106/274 (38%), Positives = 139/274 (50%), Gaps = 23/274 (8%) Query: 26 PQRSSQLLLEHVLGLSGAAYYMALADPFPPEARSAWEAGVTRRAAXXXXPVQYIIGEQEF 85 P+ +++LL++V + + P E R + RR PV YI+G +EF Sbjct: 163 PKNEARMLLQYVSEYTRVQLLTRGGEEMPDEVRQRADRLAQRR--LNGEPVAYILGVREF 220 Query: 86 YGRVFEVTPDVLIPRPETELLVEAILKYGAELWPDGLVAAPGGAARPLTAVDIGAGSGAI 145 YGR F V P VLIPRPETE LVEA+L A L +G V D+G GSGA+ Sbjct: 221 YGRRFTVNPSVLIPRPETEHLVEAVL---ARLPENGRVW------------DLGTGSGAV 265 Query: 146 SVTLAAEAPAWRVCAGDISPGALAVAGRNAQRHGTAVDLRLGDLLE---PFAGMETDILV 202 +VT+A E P V A DISP AL A +NA G V+ G + P G + DI+V Sbjct: 266 AVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWFDTDMPSEG-KWDIIV 324 Query: 203 SNPPYIPGGDIAGLQREVRDHEPRTALDGGDDGLDPYRRMMEQLPLLPAPPRLVGFELGQ 262 SNPPYI GD LQ ++R EP+ AL DGL R + + P A + E G Sbjct: 325 SNPPYIENGDKHLLQGDLR-FEPQIALTDFSDGLSCIRTLAQGAPDRLAEGGFLLLEHGF 383 Query: 263 GQAGQVAKLLRAAGHWSEIVTIDDLAGIPRHVLG 296 Q V +L G +S + T+ DLAG+ R LG Sbjct: 384 DQGAAVRGVLAENG-FSGVETLPDLAGLDRVTLG 416 >NMA0369 [J] COG2890 Methylase of polypeptide chain release factors Length = 196 Score = 129 bits (324), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 0/274 (0%), Positives = 0/274 (0%), Gaps = 23/274 (8%) Query: 26 PQRSSQLLLEHVLGLSGAAYYMALADPFPPEARSAWEAGVTRRAAXXXXPVQYIIGEQEF 85 Q + LG A PE + + Sbjct: 13 DQVPYLIEELMELGAKNVHVIPAFTKKGRPEYIFLIDGEEETL--DSLAEFMALETGTLG 70 Query: 86 YGRVFEVTPDVLIPRPETELLVEAILKYGAELWPDGLVAAPGGAARPLTAVDIGAGSGAI 145 + + L +G + Sbjct: 71 VRLLKTEHYPFNYELRKLCLSFRDEN---DLPLWEGEID------------LKIVIGKDQ 115 Query: 146 SVTLAAEAPAWRVCAGDISPGALAVAGRNAQRHGTAVDLRLGDLLE---PFAGMETDILV 202 A A + G + Sbjct: 116 KPLSARVEYEELKELAGRVREAGLKLSMYEIKELIEERALKGIKDFTINFNDS-GPGLDN 174 Query: 203 SNPPYIPGGDIAGLQREVRDHEPRTALDGGDDGLDPYRRMMEQLPLLPAPPRLVGFELGQ 262 + + + Sbjct: 175 EPLSPVSKKLYCEDKTKTC- 233 Query: 263 GQAGQVAKLLRAAGHWSEIVTIDDLAGIPRHVLG 296 Sbjct: 234 - 266 >alr0115 [J] COG2890 Methylase of polypeptide chain release factors Length = 359 Score = 121 bits (303), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 18/231 (7%), Positives = 34/231 (14%), Gaps = 21/231 (9%) Query: 75 PVQYIIGEQEFYGRVFEVTPDVLIPRPETELLVEAILKYGAELWPDGLVAAPGGAARPLT 134 + + + + L E L V Sbjct: 83 DARDLPTISAQHFHTAVHNEEQLAALEEASLDE------------PVTVWMKLDTGMHRL 130 Query: 135 A-----VDIGAGSGAISVTLAAEAPAWRVCAGDISPGALAVAGRNAQRHGTAVDLRL--G 187 + + A P A + A + Sbjct: 131 GVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQRS 190 Query: 188 DLLEPFAGMETDI--LVSNPPYIPGGDIAGLQREVRDHEPRTALDGGDDGLDPYRRMMEQ 245 + P I G R + L R Sbjct: 191 IAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVREHKAG 250 Query: 246 LPLLPAPPRLVGFELGQGQAGQVAKLLRAAGHWSEIVTIDDLAGIPRHVLG 296 P+ + + G S + + +P Sbjct: 251 EPVGYGGTWVSERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGRV 301 >SMc02435 [J] COG2890 Methylase of polypeptide chain release factors Length = 293 Score = 119 bits (299), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 91/245 (37%), Positives = 127/245 (51%), Gaps = 17/245 (6%) Query: 51 DPFPPEARSAWEAGVTRRAAXXXXPVQYIIGEQEFYGRVFEVTPDVLIPRPETELLVEAI 110 +P E + A V RRAA PV I+GE+EF+G +++ + L PRP+TE+LV+ + Sbjct: 49 EPISDEDAARIRAAVERRAAHE--PVYRILGEREFFGLKLKLSKETLEPRPDTEILVDCL 106 Query: 111 LKYGAELWPDGLVAAPGGAARPLTAVDIGAGSGAISVTLAAEAPAWRVCAGDISPGALAV 170 + + +A+ G+ R VD+G G+GAI + L A R DIS ALA Sbjct: 107 IPHARR------IASSKGSCR---IVDLGTGTGAICLALLAAVLDARGLGTDISEDALAT 157 Query: 171 AGRNAQRHGTAVDLRLGDLLEP-FAGME--TDILVSNPPYIPGGDIAGLQREVRDHEPRT 227 A NA+R+G A R G + F ++ DI+VSNPPYI I L+ EVR H+P Sbjct: 158 ALENARRNGLAE--RFGTVRSKWFEAVDGRFDIIVSNPPYIRSNVIPELEPEVRYHDPVA 215 Query: 228 ALDGGDDGLDPYRRMMEQLPLLPAPPRLVGFELGQGQAGQVAKLLRAAGHWSEIVTIDDL 287 ALDGG+DGLD YR + ++G E+G Q V L A G + + DL Sbjct: 216 ALDGGEDGLDAYRAIASHAGRHLETDGVIGLEIGFDQKRAVTALFEAQG-FRLLSAAKDL 274 Query: 288 AGIPR 292 G R Sbjct: 275 GGNDR 279 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.138 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,095,482 Number of extensions: 1053916 Number of successful extensions: 3734 Number of sequences better than 1.0e-05: 37 Number of HSP's gapped: 3659 Number of HSP's successfully gapped: 37 Length of query: 299 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 193 Effective length of database: 26,153,027 Effective search space: 5047534211 Effective search space used: 5047534211 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)