BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0164.0014.p (351 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1579 [C] COG2180 Nitrate reductase delta subunit 197 2e-50 ZprfB [J] COG1186 Protein chain release factor B 184 1e-46 AGc2479 [J] COG1186 Protein chain release factor B 184 2e-46 prfB [J] COG1186 Protein chain release factor B 184 2e-46 ZprfB [J] COG1186 Protein chain release factor B 184 2e-46 >STM1579 [C] COG2180 Nitrate reductase delta subunit Length = 346 Score = 197 bits (500), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 0/334 (0%), Positives = 0/334 (0%), Gaps = 15/334 (4%) Query: 9 EKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMMQGEKLDDE 68 + + E L + K+ E Sbjct: 36 LDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSG--PSR 93 Query: 69 MKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAALFASDLYR 128 + + L + + ++ + Sbjct: 94 NNDKGIRPDQALDRKNIREALHD----SLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWT 149 Query: 129 MYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQRIPTTESG 188 L + + V + Sbjct: 150 DSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYS 209 Query: 189 GRIHTSTSTVSVMPEAEE-FEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRVTHVPTGI 247 + ++ + + E + T + R T Sbjct: 210 LLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQ 269 Query: 248 VATCQDGKSQNSNKEKALQVLRARISDM---KRQEEEAKYAGERKSKVGTGDRSERIRTY 304 + + A + + + Sbjct: 270 TQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQL-KTNIE---SLHLE 325 Query: 305 NF-PQSRVTDHRIGLTLHRLDQVMNGDITEIISA 337 Sbjct: 326 LSE 359 >ZprfB [J] COG1186 Protein chain release factor B Length = 365 Score = 184 bits (468), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 109/335 (32%), Positives = 192/335 (57%), Gaps = 10/335 (2%) Query: 6 DRYEKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMMQGEKL 65 +R E+++ L PDV ++ ++ + KE+S L+ + + K +E++ + E Sbjct: 32 ERLEEVNAELEQPDVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEAD 91 Query: 66 DDEMKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAALFASD 125 D+E E++ L + LE + ++I+ +GG EA +AS Sbjct: 92 DEETFNEAVAELDALEEKLAQLEFR----RMFSGEYDSADCYLDIQAGSGGTEAQDWASM 147 Query: 126 LYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQRIPTT 185 L RMY R+A+++G++ E+++ + ++ G K V I+G AY ++ E+G HR+ R Sbjct: 148 LERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPF 207 Query: 186 ESGGRIHTSTSTVSVMPEAEE-FEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRVTHVP 244 +SGGR HTS S+ V PE ++ +IEI D+R+D + +SGAGGQ VN T+SAVR+TH+P Sbjct: 208 DSGGRRHTSFSSAFVYPEVDDDIDIEINPADLRIDVYRTSGAGGQHVNRTESAVRITHIP 267 Query: 245 TGIVATCQDGKSQNSNKEKALQVLRARISDMKRQEEEAKYAG--ERKSKVGTGDRSERIR 302 TGIV CQ+ +SQ+ NK++A++ ++A++ +++ Q++ A+ + KS +G G +IR Sbjct: 268 TGIVTQCQNDRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWG---SQIR 324 Query: 303 TYNFPQSRVTDHRIGLTLHRLDQVMNGDITEIISA 337 +Y SR+ D R G+ V++G + + I A Sbjct: 325 SYVLDDSRIKDLRTGVETRNTQAVLDGSLDQFIEA 359 >AGc2479 [J] COG1186 Protein chain release factor B Length = 342 Score = 184 bits (467), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 97/234 (41%), Positives = 145/234 (61%), Gaps = 7/234 (2%) Query: 108 VEIRGAAGGDEAALFASDLYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAY 167 VE+ AGG E+ +A+ L RMYTR+A+ QG++VE+++++ D G K ++ G A+ Sbjct: 94 VEVHSGAGGTESQDWANMLLRMYTRWAERQGFKVEVLEIHDGDEAGIKSATILVKGHNAF 153 Query: 168 SKMKFESGAHRVQRIPTTESGGRIHTSTSTVSVMPEAEE-FEIEILDKDIRVDTFCSSGA 226 MK ESG HR+ RI +S R HTS S++ V P ++ +I+I + D R+DT+ SSGA Sbjct: 154 GWMKTESGVHRLVRISPYDSNARRHTSFSSIWVYPVVDDSIQIDINESDCRIDTYRSSGA 213 Query: 227 GGQSVNTTKSAVRVTHVPTGIVATCQDGKSQNSNKEKALQVLRARI--SDMKRQEEEAKY 284 GGQ VNTT SAVR+TH+PTGI CQ +SQ+ N+ KA +LRAR+ +++++EE A Sbjct: 214 GGQHVNTTDSAVRITHIPTGIAVACQQERSQHKNRAKAWDMLRARLYEVELQKREEAANA 273 Query: 285 AGERKSKVGTGDRSERIRTYNF-PQSRVTDHRIGLTLHRLDQVMNGDITEIISA 337 K+ +G G +IR+Y P V D R G+ V++GD+ E + A Sbjct: 274 QAASKTDIGWG---HQIRSYVLQPYQLVKDLRTGVESTAPGNVLDGDLNEFMEA 324 >prfB [J] COG1186 Protein chain release factor B Length = 365 Score = 184 bits (467), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 109/335 (32%), Positives = 192/335 (57%), Gaps = 10/335 (2%) Query: 6 DRYEKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMMQGEKL 65 +R E+++ L PDV ++ ++ + KE+S L+ + + K +E++ + E Sbjct: 32 ERLEEVNAELEQPDVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEAD 91 Query: 66 DDEMKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAALFASD 125 D+E E++ L + LE + ++I+ +GG EA +AS Sbjct: 92 DEETFNEAVAELDALEEKLAQLEFR----RMFSGEYDSADCYLDIQAGSGGTEAQDWASM 147 Query: 126 LYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQRIPTT 185 L RMY R+A+++G++ E+++ + ++ G K V I+G AY ++ E+G HR+ R Sbjct: 148 LERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPF 207 Query: 186 ESGGRIHTSTSTVSVMPEAEE-FEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRVTHVP 244 +SGGR HTS S+ V PE ++ +IEI D+R+D + +SGAGGQ VN T+SAVR+TH+P Sbjct: 208 DSGGRRHTSFSSAFVYPEVDDDIDIEINPADLRIDVYRTSGAGGQHVNRTESAVRITHIP 267 Query: 245 TGIVATCQDGKSQNSNKEKALQVLRARISDMKRQEEEAKYAG--ERKSKVGTGDRSERIR 302 TGIV CQ+ +SQ+ NK++A++ ++A++ +++ Q++ A+ + KS +G G +IR Sbjct: 268 TGIVTQCQNDRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWG---SQIR 324 Query: 303 TYNFPQSRVTDHRIGLTLHRLDQVMNGDITEIISA 337 +Y SR+ D R G+ V++G + + I A Sbjct: 325 SYVLDDSRIKDLRTGVETRNTQAVLDGSLDQFIEA 359 Score = 184 bits (467), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 109/335 (32%), Positives = 192/335 (57%), Gaps = 10/335 (2%) Query: 6 DRYEKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMMQGEKL 65 +R E+++ L PDV ++ ++ + KE+S L+ + + K +E++ + E Sbjct: 32 ERLEEVNAELEQPDVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEAD 91 Query: 66 DDEMKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAALFASD 125 D+E E++ L + LE + ++I+ +GG EA +AS Sbjct: 92 DEETFNEAVAELDALEEKLAQLEFR----RMFSGEYDSADCYLDIQAGSGGTEAQDWASM 147 Query: 126 LYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQRIPTT 185 L RMY R+A+++G++ E+++ + ++ G K V I+G AY ++ E+G HR+ R Sbjct: 148 LERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPF 207 Query: 186 ESGGRIHTSTSTVSVMPEAEE-FEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRVTHVP 244 +SGGR HTS S+ V PE ++ +IEI D+R+D + +SGAGGQ VN T+SAVR+TH+P Sbjct: 208 DSGGRRHTSFSSAFVYPEVDDDIDIEINPADLRIDVYRTSGAGGQHVNRTESAVRITHIP 267 Query: 245 TGIVATCQDGKSQNSNKEKALQVLRARISDMKRQEEEAKYAG--ERKSKVGTGDRSERIR 302 TGIV CQ+ +SQ+ NK++A++ ++A++ +++ Q++ A+ + KS +G G +IR Sbjct: 268 TGIVTQCQNDRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWG---SQIR 324 Query: 303 TYNFPQSRVTDHRIGLTLHRLDQVMNGDITEIISA 337 +Y SR+ D R G+ V++G + + I A Sbjct: 325 SYVLDDSRIKDLRTGVETRNTQAVLDGSLDQFIEA 359 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.129 0.348 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,068,289 Number of extensions: 905687 Number of successful extensions: 1727 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 1697 Number of HSP's successfully gapped: 23 Length of query: 351 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 244 Effective length of database: 26,037,693 Effective search space: 6353197092 Effective search space used: 6353197092 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 116 (49.3 bits)