BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0164.0011.p (377 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all4591 [C] COG1304 L-lactate dehydrogenase (FMN-dependent) and ... 262 6e-70 NMA0604 [R] COG1040 Predicted amidophosphoribosyltransferases 202 6e-52 mlr6371 [C] COG1304 L-lactate dehydrogenase (FMN-dependent) and ... 192 5e-49 VNG6445G [C] COG1304 L-lactate dehydrogenase (FMN-dependent) and... 186 5e-47 VNG6081G [C] COG1304 L-lactate dehydrogenase (FMN-dependent) and... 186 5e-47 >all4591 [C] COG1304 L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases Length = 350 Score = 262 bits (670), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 137/323 (42%), Positives = 190/323 (58%), Gaps = 2/323 (0%) Query: 35 SPTTGERKIEHVRLCLNEEVGASGITAGFEHYRFRHNALPELNYDDISLRTVFLGRELRT 94 S T RK +H+R+CL E+V T G E YRF H+ LPE++ +DI L FLG++L Sbjct: 9 SAQTQSRKADHIRICLEEDVQFRDTTNGLERYRFTHSCLPEIDRNDIDLSATFLGKKLNA 68 Query: 95 PLLISSMTGGSAATGAINARLAEAAERRGWALGVGSIRAAIERSELAASFRVRDKAPGIP 154 PLLISSMTGG+ G IN RLA A+ A+GVGS R A+E+ ++A +F +R AP + Sbjct: 69 PLLISSMTGGTEEAGIINQRLAGLAQHYKLAMGVGSQRVAVEKPQVADTFAIRKYAPDVL 128 Query: 155 VIANIGAVQLSYGFGVEECRRAVEIAGADWLVLHLNGIQEVFQPEGNTGFADLLTRIERV 214 + AN+GAVQL+Y +G++EC R +++ AD L+LH+N +QE QP G+ F LL +I ++ Sbjct: 129 LFANVGAVQLNYKYGLDECLRIIDMLEADALILHINPLQECIQPRGDVNFRGLLDKINQL 188 Query: 215 CRELEIPVGVKEVGWGIDGETAIRLYXXXXXXXXXXXXXXTSWSQVEKFRSSDPVRRAAA 274 C +L +P KEVG GI G A +L TSW++VE R+ + ++R Sbjct: 189 CSKLPVPAIAKEVGNGISGAMAEKLIAAGVQAIDVAGAGGTSWAKVEGERAENAMQRRLG 248 Query: 275 EAFADWGIPTAQCIAEVRAASKHGALIGSGGLRHGVXXXXXXXXXXXXXXFGRNLLGPAV 334 FADWG+PTA+CI VRA + H LI SGGLR G+ L AV Sbjct: 249 RTFADWGMPTAECITSVRAIAPHIPLIASGGLRDGLDVAKAIALGADIAGLAMPFLQAAV 308 Query: 335 ASEETLDQALAQVEL-ELRTAMF 356 SE L Q L +V + E+ T +F Sbjct: 309 ESEAAL-QDLTEVLIAEITTVLF 330 >NMA0604 [R] COG1040 Predicted amidophosphoribosyltransferases Length = 365 Score = 202 bits (515), Expect = 6e-52, Method: Compositional matrix adjust. Identities = 130/324 (40%), Positives = 174/324 (53%), Gaps = 8/324 (2%) Query: 37 TTGERKIEHVRLCLNEEVGASGITAGFEHYRFRHNALPELNYDDISLRTVFLGRELRTPL 96 TT RKIEH++LC V A G++AGFE H ALPELN D++ L FLG+ ++ P Sbjct: 4 TTSRRKIEHLKLCAESPVEARGVSAGFEDVTLIHRALPELNMDELDLTVDFLGKRMQAPF 63 Query: 97 LISSMTGGSAATGAINARLAEAAERRGWALGVGSIRAAIERSELAASFR-VRDKAPGIPV 155 LI+S+TGG T +NA LA AAE G +GVGS RAAI+ SFR VRDKAP V Sbjct: 64 LIASITGGHPDTLPVNAALAAAAEELGVGIGVGSQRAAIDDPAQEDSFRVVRDKAPNAFV 123 Query: 156 IANIGAVQLSYGFGVEECRRAVEIAGADWLVLHLNGIQEVFQPEGNTGFADLLTRIERVC 215 N+GA Q+ +GVE + +E+ AD L +HLN +QE QPEG+ L I +C Sbjct: 124 YGNVGAAQIRQ-YGVEGVEKLIEMIDADALAIHLNFLQEAIQPEGDRDATGCLDMIAEIC 182 Query: 216 RELEIPVGVKEVGWGIDGETAIRLYXXXXXXXXXXXXXXTSWSQVEKFR---SSDPVRRA 272 + IPV KE G GI E A+ L+ TSW+ VE +R S DPV Sbjct: 183 SMVRIPVIAKETGAGISREDALLLHKAGVSAIDVGGVGGTSWAGVEVYRAKESKDPVSER 242 Query: 273 AAEAFADWGIPTAQCIAEVRAASKHGALIGSGGLRHGVXXXXXXXXXXXXXXFGRNLLGP 332 E F D+GIPT + E R + LI +GG+R G+ +GP Sbjct: 243 LGELFWDFGIPTVASLIESRVSLP---LIATGGVRTGLDIAKSIALGASAASAALPFVGP 299 Query: 333 AVASEETLDQALAQVELELRTAMF 356 ++ +E++ + L+ + E R AMF Sbjct: 300 SLEGKESVVKVLSCMLDEFRAAMF 323 >mlr6371 [C] COG1304 L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases Length = 351 Score = 192 bits (489), Expect = 5e-49, Method: Compositional matrix adjust. Identities = 129/331 (38%), Positives = 176/331 (53%), Gaps = 5/331 (1%) Query: 41 RKIEHVRLCLNEEVGASGITAGFEHYRFRHNALPELNYDDISLRTVFLGRELRTPLLISS 100 RK +H+ + L+ + + AG+E+ RF H ALPEL+ I LR LG+ +R PLLISS Sbjct: 11 RKDDHLDIVLDRRTAPATVAAGWEYIRFEHCALPELDLTQIDLRASLLGKTMRAPLLISS 70 Query: 101 MTGGSAATGAINARLAEAAERRGWALGVGSIRAAIE-RSELAASFRVRDKAPGIPVIANI 159 MTGG AIN L+EAA+ G A+ VGS R +++ R+ + +R AP IP++ANI Sbjct: 71 MTGGVLRAEAINRHLSEAAQALGIAMCVGSQRVSLQSRNSQGLTRALRRMAPDIPLLANI 130 Query: 160 GAVQLSYGFGVEECRRAVEIAGADWLVLHLNGIQEVFQPEGNTGFADLLTRIERVCRELE 219 GA QL G++ RAV+ AD L++HLN +QE QPEG+ + +L +I R R ++ Sbjct: 131 GAAQLREADGLDLACRAVDALEADGLIVHLNALQEAVQPEGDRDWRGVLAQIARAARSVD 190 Query: 220 IPVGVKEVGWGIDGETAIRLYXXXXXXXXXXXXXXTSWSQVEKFRSSDPVRRAAAEAFAD 279 +P+ KEVG G+ A L TSW+ VE R+ D RA A AFAD Sbjct: 191 VPIVAKEVGSGLSASVACALVKAGVAVIDVAGAGGTSWAAVEGERARDAADRAVAMAFAD 250 Query: 280 WGIPTAQCIAEVRAASKHGALIGSGGLRHGVXXXXXXXXXXXXXXFGRNLLGPAVASEET 339 WGIPT + VR A LI SGG+R GV +L A S E Sbjct: 251 WGIPTPASVQAVRRALPTVKLIASGGIRDGVDVAKAIRLGADIAGQAAGVLRAATVSTEA 310 Query: 340 LDQALAQVELELRT-AMFGIXXXXPDLAALR 369 + +A E+ +R A+ DLAALR Sbjct: 311 V---VAHFEIVIRQLAVACFCTGSADLAALR 338 >VNG6445G [C] COG1304 L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases Length = 360 Score = 186 bits (472), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 121/332 (36%), Positives = 168/332 (50%), Gaps = 19/332 (5%) Query: 35 SPTTGERKIEHVRLCLNEEVGASGITAGFEHYRFRHNALPELNYDDISLRTVFLGRELRT 94 S T +RK +H+++ +V +G GF+ HNALPEL+YD I FLG +L Sbjct: 6 STQTEDRKDDHLQIVQERDVETTG--TGFDDVHLVHNALPELDYDAIDPSIDFLGHDLSA 63 Query: 95 PLLISSMTGGSAATGAINARLAEAAERRGWALGVGSIRAAIERSE---LAASFRVRDKAP 151 P+ I SMTGG T IN LA AA G A+G+GS RA +E + L + VRD AP Sbjct: 64 PIFIESMTGGHHNTTEINRALARAASETGIAMGLGSQRAGLELDDERVLESYTVVRDAAP 123 Query: 152 GIPVIANIGAVQLSYGFGVEECRRAVEIAGADWLVLHLNGIQEVFQPEGNTGFADLLTRI 211 + N+GA QL + +E +AVE+ AD L +HLN +QE QPEG+ + + I Sbjct: 124 DAFIYGNLGAAQLRE-YDIEMVEQAVEMIDADALAVHLNFLQEATQPEGDVDGRNCVAAI 182 Query: 212 ERVCRELEIPVGVKEVGWGIDGETAIRLYXXXXXXXXXXXXXXTSWSQVE---KFRSSDP 268 ERV L +P+ VKE G GI GETA L T+WS +E ++ P Sbjct: 183 ERVSEALSVPIIVKETGNGISGETARELTAAGVDALDVAGKGGTTWSGIEAYRAAAANAP 242 Query: 269 VRRAAAEAFADWGIPTA----QCIAEVRAASKHGALIGSGGLRHGVXXXXXXXXXXXXXX 324 ++ F +WGIPTA +C+AE H +I SGG+R G+ Sbjct: 243 RQKQIGTLFREWGIPTAASTIECVAE------HDCVIASGGVRTGLDVAKAIALGARAGG 296 Query: 325 FGRNLLGPAVASEETLDQALAQVELELRTAMF 356 + L PA + + + + + ELRTAMF Sbjct: 297 LAKPFLKPATDGPDAVIERVGDLIAELRTAMF 328 >VNG6081G [C] COG1304 L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases Length = 360 Score = 186 bits (472), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 121/332 (36%), Positives = 168/332 (50%), Gaps = 19/332 (5%) Query: 35 SPTTGERKIEHVRLCLNEEVGASGITAGFEHYRFRHNALPELNYDDISLRTVFLGRELRT 94 S T +RK +H+++ +V +G GF+ HNALPEL+YD I FLG +L Sbjct: 6 STQTEDRKDDHLQIVQERDVETTG--TGFDDVHLVHNALPELDYDAIDPSIDFLGHDLSA 63 Query: 95 PLLISSMTGGSAATGAINARLAEAAERRGWALGVGSIRAAIERSE---LAASFRVRDKAP 151 P+ I SMTGG T IN LA AA G A+G+GS RA +E + L + VRD AP Sbjct: 64 PIFIESMTGGHHNTTEINRALARAASETGIAMGLGSQRAGLELDDERVLESYTVVRDAAP 123 Query: 152 GIPVIANIGAVQLSYGFGVEECRRAVEIAGADWLVLHLNGIQEVFQPEGNTGFADLLTRI 211 + N+GA QL + +E +AVE+ AD L +HLN +QE QPEG+ + + I Sbjct: 124 DAFIYGNLGAAQLRE-YDIEMVEQAVEMIDADALAVHLNFLQEATQPEGDVDGRNCVAAI 182 Query: 212 ERVCRELEIPVGVKEVGWGIDGETAIRLYXXXXXXXXXXXXXXTSWSQVE---KFRSSDP 268 ERV L +P+ VKE G GI GETA L T+WS +E ++ P Sbjct: 183 ERVSEALSVPIIVKETGNGISGETARELTAAGVDALDVAGKGGTTWSGIEAYRAAAANAP 242 Query: 269 VRRAAAEAFADWGIPTA----QCIAEVRAASKHGALIGSGGLRHGVXXXXXXXXXXXXXX 324 ++ F +WGIPTA +C+AE H +I SGG+R G+ Sbjct: 243 RQKQIGTLFREWGIPTAASTIECVAE------HDCVIASGGVRTGLDVAKAIALGARAGG 296 Query: 325 FGRNLLGPAVASEETLDQALAQVELELRTAMF 356 + L PA + + + + + ELRTAMF Sbjct: 297 LAKPFLKPATDGPDAVIERVGDLIAELRTAMF 328 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,299,297 Number of extensions: 979589 Number of successful extensions: 1798 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 1759 Number of HSP's successfully gapped: 15 Length of query: 377 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 269 Effective length of database: 25,922,359 Effective search space: 6973114571 Effective search space used: 6973114571 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)