BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0164.0008.p (498 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2858 [I] COG1502 Phosphatidylserine/phosphatidylglycerophospha... 536 e-152 BS_ywiE [I] COG1502 Phosphatidylserine/phosphatidylglycerophosph... 369 e-102 BS_ywnE [I] COG1502 Phosphatidylserine/phosphatidylglycerophosph... 346 5e-95 lin2646 [I] COG1502 Phosphatidylserine/phosphatidylglycerophosph... 342 8e-94 lin0008 [I] COG1502 Phosphatidylserine/phosphatidylglycerophosph... 328 1e-89 >BH2858 [I] COG1502 Phosphatidylserine/phosphatidylglycerophosphate/cardiol ipin synthases and related enzymes Length = 184 Score = 536 bits (1381), Expect = e-152, Method: Compositional matrix adjust. Identities = 0/477 (0%), Positives = 0/477 (0%), Gaps = 4/477 (0%) Query: 26 GTIVSVFS---TLTVISIGLGIFMENRNPSTTMSWILLLALIPVVGLILYFLFGQNVFKR 82 ++ T + + T + W L + L++ + Sbjct: 27 CELIFEDKISGTKSERPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVVLVEELRERGIN 86 Query: 83 RKYDKKAQRDQMAYERIENDALRMHQDWSIFDTTRQKLLGLSQRLARTPISFNSETRIXX 142 + + R + + + GL A+ I Sbjct: 87 FRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTKAGLETARAQGRIGGRRPKLTPE 146 Query: 143 XXXXXXXXXXXXXRQAQHHIHMEYYIFRADHIGTRIQQILIEKARAGVAVRFMYDAVGSI 202 Sbjct: 147 206 Query: 203 QLSRAFLKELSDAGVQVAAYGN-STSFFSSRVNYRNHRKIVVIDGDVGFMGGLNVGDEYL 261 Sbjct: 207 266 Query: 262 SRSKTYGFWRDTHMLLRGEAVRTMQIIFLQDWMHTTGEKILEQDYLSPQLRFTNGDGAVQ 321 Sbjct: 267 326 Query: 322 IIASGPDNERRALKNIFFSMITSAEKSVWIATPYFIPDEDILTALRVAAMSGLDVRLLFP 381 Sbjct: 327 386 Query: 382 AKPDKWLPFLASHSYFPALLESGVKIYEYEKGFIHSKLLITDGEIATIGTANMDMRSFHL 441 Sbjct: 387 446 Query: 442 NFEVNALLLQTESVSRIVADFERDLLSTRQIVHETFMNKRLLERVLESAARLMSPLL 498 Sbjct: 447 503 >BS_ywiE [I] COG1502 Phosphatidylserine/phosphatidylglycerophosphate/cardiol ipin synthases and related enzymes Length = 184 Score = 369 bits (947), Expect = e-102, Method: Compositional matrix adjust. Identities = 0/476 (0%), Positives = 0/476 (0%), Gaps = 23/476 (4%) Query: 35 LTVISIGL----GIFMENRNPSTTMSWILLLALIPVVGLILYFLFGQ-----NVFK-RRK 84 S + + + + + L ++L + Sbjct: 36 SGTKSERPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVVLVEELRERGINFRSLTDSID 95 Query: 85 YDKKAQRDQMAYERIENDALRMHQDWSIFDTTRQKLLGLSQRLARTPISFNSETRIXXXX 144 R + + A + ++ + ++ Sbjct: 96 TSTPMGRF--FFHVMGALAEMEREL------IVERTKAGLETARAQGRIGGRRPKLTPEQ 147 Query: 145 XXXXXXXXXXXRQAQHHIHMEYYIFRADHIGTRIQQILIEKARAGVAVRFMYDAVGSIQL 204 Sbjct: 148 207 Query: 205 SRAFLKELSDAGVQVAAYGN-STSFFSSRVNYRNHRKIVVIDGDVGFMGGLNVGDEYLSR 263 Sbjct: 208 267 Query: 264 SKTYGFWRDTHMLLRGEAVRTMQIIFLQDWMHTTGEKILEQD-YLSPQLRFTNGDGAVQI 322 Sbjct: 268 -- 325 Query: 323 IASGPDNERRALKNIFFSMITSAEKSVWIATPYFIPDEDILTALRVAAMSGLDVRLLFPA 382 Sbjct: 326 - 384 Query: 383 KPDKWLPFLASHSYFPALLESGVKIYEYEKGFIHSKLLITDGEIATIGTANMDMRSFHLN 442 Sbjct: 385 444 Query: 443 FEVNALLLQTESVSRIVADFERDLLSTRQIVHETFMNKRLLERVLESAARLMSPLL 498 Sbjct: 445 500 >BS_ywnE [I] COG1502 Phosphatidylserine/phosphatidylglycerophosphate/cardiol ipin synthases and related enzymes Length = 184 Score = 346 bits (888), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 0/496 (0%), Positives = 0/496 (0%), Gaps = 35/496 (7%) Query: 23 STVGTIVSVFSTLTVISIGLGIFMENRNPSTTMSWILLLALIPVVGLILYFLFGQNVFKR 82 VS T + + L+ G + Sbjct: 2 LIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLRTLSAGDTL-VV 60 Query: 83 RKYDKKAQRDQMAYERIENDALR-------MHQDWSIFDTTRQKLLGLSQRLARTPISFN 135 K D+ + + +E R + + Sbjct: 61 WKLDRLGRSMRHLVVLVEELRERGINFRSLTDSIDTSTPM--GRFFFHVMGALAEMEREL 118 Query: 136 SETRIXXXXXXXXXXXXXXXRQAQHHIHMEYYIFRADHIGTRIQQILIEKARAGVAVRFM 195 R R+ + R GT Q++ I + Sbjct: 119 IVERTKAGLETARAQGRIGGRRPKLTPEQWAQAGRLIAAGTPRQKVAIIYDVGVSTLYKR 178 Query: 196 YDAVGSIQLSRAFLKELSDAGVQVAAYGNSTSFFSS-------RVNYRNHRKIVVIDGDV 248 Sbjct: 179 ------ 232 Query: 249 GFMGGLNVGDEYLSRSKTYGFWRDTHMLLRGEAVRTMQIIFLQDWMHTTGEKILE----- 303 Sbjct: 233 292 Query: 304 -QDYLSPQLRFTNGDGAVQIIASGPDNERRALKNIFFSMITSAEKSVWIATPYFIPDEDI 362 Sbjct: 293 ------ 346 Query: 363 LTALRVAAMSGLDVRLLFPAKPDKWLPFLASHSYFPALLESGVKIYEYEKGFIHSKLLIT 422 Sbjct: 347 406 Query: 423 DGEIATIGTANMDMRSFHLNFEVNALLLQTESVSRIVADFERDLLSTRQIVHETFMNKRL 482 Sbjct: 407 466 Query: 483 LERVLESAARLMSPLL 498 Sbjct: 467 482 >lin2646 [I] COG1502 Phosphatidylserine/phosphatidylglycerophosphate/cardiol ipin synthases and related enzymes Length = 184 Score = 342 bits (877), Expect = 8e-94, Method: Compositional matrix adjust. Identities = 0/462 (0%), Positives = 0/462 (0%), Gaps = 9/462 (1%) Query: 44 IFMENRNPSTTMSWILLLALIPVVGLILYFLFGQNVFKRRKYDKKAQRDQMAYERIENDA 103 + L+ G + + + E Sbjct: 23 NCAGCELIFEDKISGTKSERPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVVLVEELRE 82 Query: 104 LRMHQ-DWSIFDTTRQKLLGLSQRLARTP---ISFNSETRIXXXXXXXXXXXXXXXRQAQ 159 ++ + T + + R R+ + Sbjct: 83 RGINFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTKAGLETARAQGRIGGRRPK 142 Query: 160 HHIHMEYYIFRADHIGTRIQQILIEKARAGVAVRFMYDAVGSIQLSRAFLKELSDAGVQV 219 Sbjct: 143 202 Query: 220 AAYGNST-SFFSSRVNYRNHRKIVVIDGDVGFMGGLNVGDEYLSRSKTYGFWRDTHMLLR 278 Sbjct: 203 262 Query: 279 GEAVRTMQIIFLQDWMHTTGEKILEQDYLSPQLRFTNGDG--AVQIIASGPDNERRALKN 336 Sbjct: 263 -- 320 Query: 337 IFFSMITSAEKSVWIATPYFIPDEDILTALRVAAMSGLDVRLLFPAKPDKWLPFLASHSY 396 Sbjct: 321 380 Query: 397 FPALLESGVKIYEYEKGFIHSKLLITDGEIATIGTANMDMRSFHLNFEVNALLLQTESVS 456 Sbjct: 381 440 Query: 457 RIVADFERDLLSTRQIVHETFMNKRLLERVLESAARLMSPLL 498 Sbjct: 441 482 >lin0008 [I] COG1502 Phosphatidylserine/phosphatidylglycerophosphate/cardiol ipin synthases and related enzymes Length = 184 Score = 328 bits (842), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 0/479 (0%), Positives = 0/479 (0%), Gaps = 31/479 (6%) Query: 36 TVISIGLGIFMENRNPSTTMSWILLLALIPVVGLILYFLFGQNVFKRRKYDKKAQRDQMA 95 + + T + W L + L++ + + Sbjct: 41 ERPGLKKLLR-TLSAGDTLVVWKLDRLGRSMRHLVVLVEELRERGINF----------RS 89 Query: 96 YERIENDALRMHQDWSIFDTTRQKLLGLSQRLAR----TPISFNSETRIXXXXXXXXXXX 151 + + M + + ++ Sbjct: 90 LTDSIDTSTPMGRFFFHVMGALAEMERELIVERTKAGLETARAQ-GRIGGRRPKLTPEQW 148 Query: 152 XXXXRQAQHHIHMEYYIFRADHIGTRIQQILIEKARAGVAVRFMYDAVGSIQLSRAFLKE 211 Sbjct: 149 208 Query: 212 LSDAGVQVAAYGNSTSFFSSR-VNYRNHRKIVVIDGDVGFMGGLNVGDEYLSRSKTYGFW 270 Sbjct: 209 268 Query: 271 RDTHMLLRGEAVRTMQIIFLQDWMHTTGEK------ILE---QDYLSPQLRFTNGDGAVQ 321 Sbjct: 269 --- 325 Query: 322 IIASGPDNERRALKNIFFSMITSAEKSVWIATPYFIPDEDILTALRVAAMSGLDVRLLFP 381 Sbjct: 326 385 Query: 382 AKPDKWLPFLASHSYFPALLESGVKIYEY-EKGFIHSKLLITDGEIATIGTANMDMRSFH 440 Sbjct: 386 445 Query: 441 LNFEVNALLL-QTESVSRIVADFERDLLSTRQIVHETFMNKRLLERVLESAARLMSPLL 498 Sbjct: 446 504 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,861,555 Number of extensions: 1223347 Number of successful extensions: 7779 Number of sequences better than 1.0e-05: 47 Number of HSP's gapped: 7645 Number of HSP's successfully gapped: 48 Length of query: 498 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 387 Effective length of database: 25,576,357 Effective search space: 9898050159 Effective search space used: 9898050159 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 118 (50.1 bits)