BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0164.0004.p (303 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA1505 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 212 5e-55 PA3870 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 185 8e-47 MT3427 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 184 2e-46 MT3192 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 173 3e-43 Rv3109 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 173 3e-43 >PA1505 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 331 Score = 212 bits (540), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 123/305 (40%), Positives = 172/305 (56%), Gaps = 11/305 (3%) Query: 3 AEGMQFQPQDEIMSYEEITAVVKALAPLGLSKIRLTGGEPLVRKDLDKLVSMIAAIPGIE 62 +E MQF P+D+++S EE+ AV A LG+ +IR+TGGEPLVRK + L++ + +E Sbjct: 34 SEDMQFLPRDQVLSLEELYAVADAFIGLGVRRIRITGGEPLVRKGITGLLARLGQRAELE 93 Query: 63 DISLTTNGLMLPAKAALLKQAGLSRVNISLDSLRQDRFAMITRGGEVAKVLKGIEAAEAA 122 D+++TTNG L +AA LK AG+ R+N+SLDSL+++RFA TR + +VL GI+AA A Sbjct: 94 DLAITTNGSQLRERAAELKAAGVRRLNVSLDSLQRERFAAFTRSDRLEQVLDGIQAAREA 153 Query: 123 GLSPIKLNVVLMKGINDDEIKDFISLTLNSPLNVRFIEYMPIGSASDAWRQTYLPLETVV 182 G IKLN V+ KG NDDEI D + L + L++ FIE MP+GS + R+ L V Sbjct: 154 GFERIKLNCVVQKGRNDDEILDLVEYALANQLDISFIEEMPLGSITSHRRELTLCTSDEV 213 Query: 183 EACHEAGWATEEADMPSGNGPSQNRRVVGARGTFGLIHPVSEHFCDNCNRLRLTADGHIK 242 A E W + SG GPS+ ++ G I P S +FC +CNR+R+TA+G + Sbjct: 214 RAIIERRWPLTPSLARSG-GPSRYYQIGDEPSRIGFISPHSNNFCGDCNRVRVTAEGKLV 272 Query: 243 ACLYWADEYNVRPLT----SDPAAVQALFRQALGNKPHNHEMALALERKAQSHTPTVRRM 298 CL ++R L D + A AL KP H AQ +R M Sbjct: 273 LCLGHEGALDLRELLRSHPGDRERLSAALVAALNLKPERHHF------DAQEQVQVLRFM 326 Query: 299 SQIGG 303 S GG Sbjct: 327 SMTGG 331 >PA3870 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 329 Score = 185 bits (469), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 117/307 (38%), Positives = 168/307 (54%), Gaps = 15/307 (4%) Query: 3 AEGMQFQPQDEIMSYEEITAVVKALAPLGLSKIRLTGGEPLVRKDLDKLVSMIAAIPGIE 62 AE M+F P+ ++++ EEI V + G+ K+RLTGGEPLVR + L +AA+PG+ Sbjct: 32 AEDMRFLPRQQVLTLEEIERVARLFVAGGVRKLRLTGGEPLVRPGIVGLCERLAALPGLR 91 Query: 63 DISLTTNGLMLPAKAALLKQAGLSRVNISLDSLRQDRFAMITRGGEVAKVLKGIEAAEAA 122 ++ +T+NG L A L AGLSR+NISLD+L RF ITR GE+ KVL GI+AA+AA Sbjct: 92 ELCMTSNGSQLVRYARPLYDAGLSRLNISLDTLDPLRFRAITRNGELDKVLAGIDAAQAA 151 Query: 123 GLSPIKLNVVLMKGINDDEIKDFISLTLNSPLNVRFIEYMPIGSASDAWRQTYLPLETVV 182 G IKLN V+MKG N DE+ + + L++ FIE MP+G +++ + V Sbjct: 152 GFRRIKLNAVVMKGRNADEVPALVDFAIARGLDISFIEEMPLGQVGRERGESFCSSDEVR 211 Query: 183 EACHEAGWATEEADMPSGNGPSQNRRVVGARGT-FGLIHPVSEHFCDNCNRLRLTADGHI 241 + + A+ GP++ R+V T G I P S +FC CNRLRLT +G + Sbjct: 212 ALIAQRHALLDSAE--QSGGPARYVRLVEHPQTRIGFISPHSHNFCATCNRLRLTVEGRL 269 Query: 242 KACLYWADEYNVRPLTS-----DPAAVQALFRQALGNKPHNHEMALALERKAQSHTPTVR 296 CL ++R L D ++A+ +AL KP HE + A E +R Sbjct: 270 LLCLGHEHSLDLRALLRRHPLHDGPLLEAIG-EALQRKPARHEFSAAGE------VQVLR 322 Query: 297 RMSQIGG 303 M+ GG Sbjct: 323 FMNMSGG 329 >MT3427 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 395 Score = 184 bits (466), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 115/306 (37%), Positives = 167/306 (54%), Gaps = 15/306 (4%) Query: 1 MPAEGMQFQPQDEIMSYEEITAVVKALAPLGLSKIRLTGGEPLVRKDLDKLVSMIAAI-- 58 MP + P+ +++S +EI+ +V A +G+ KIRLTGGEPL+R DL ++ +I+A Sbjct: 81 MPEAEYAWLPRADLLSVDEISLIVDAFIAVGVDKIRLTGGEPLIRSDLAAIIEVISAKVG 140 Query: 59 --PGIEDISLTTNGLMLPAKAALLKQAGLSRVNISLDSLRQDRFAMITRGGEVAKVLKGI 116 G++D+++TTNG++L +A LK AG+ R+ ISLD+LR DRF I++ G KV++GI Sbjct: 141 DGSGLQDLAITTNGVLLADQARKLKSAGMRRITISLDTLRPDRFKAISQRGTHYKVIEGI 200 Query: 117 EAAEAAGLSPIKLNVVLMKGINDDEIKDFISLTLNSPLNVRFIEYMPIGSASDAWR--QT 174 EA AAG + KL+ V+++G NDDE+ D I N VRFIEYM +G A+ W + Sbjct: 201 EAVAAAGFTDTKLDSVVIRGFNDDELSDLIEFARNVNAEVRFIEYMDVGGATQ-WSMDKV 259 Query: 175 YLPLETVVEACHEAGWATEEADMPSGNGPSQNRRVVGARGTFGLIHPVSEHFCDNCNRLR 234 + + + + G A +P + NR + TFG+I +E FC C+R R Sbjct: 260 FTKAQMLSTLGKKYG---PIAALPKYDSAPANRYRLPDGTTFGIIASTTEPFCATCDRSR 316 Query: 235 LTADGHIKACLYWADEYNVRPLTSDPAA----VQALFRQALGNKPHNHEMALALERKAQS 290 LTADG CLY N+R A+ VQ L R E LA +R Q Sbjct: 317 LTADGIWLHCLYALSGINLRESVRAGASANDVVQILQRGWRDRANRGAEQRLA-QRTRQV 375 Query: 291 HTPTVR 296 P R Sbjct: 376 FLPVSR 381 >MT3192 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 368 Score = 173 bits (439), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 95/259 (36%), Positives = 150/259 (57%), Gaps = 8/259 (3%) Query: 1 MPAEGMQFQPQDEIMSYEEITAVVKALAPLGLSKIRLTGGEPLVRKDLDKLVSMIAAI-- 58 MP E + P+ +++S +EI+A+V +G+SK+R+TGGEPL+R DL ++V ++A Sbjct: 54 MPEEHYTWLPRQDLLSVKEISAIVDVFLSVGVSKVRITGGEPLIRPDLPEIVRTLSAKVG 113 Query: 59 --PGIEDISLTTNGLMLPAKAALLKQAGLSRVNISLDSLRQDRFAMITRGGEVAKVLKGI 116 G+ D+++TTNG++L + LK AG+ R+ +SLD+L+ +RF I++ KV+ GI Sbjct: 114 EDSGLRDLAITTNGVLLADRVDGLKAAGMKRITVSLDTLQPERFKAISQRNSHDKVIAGI 173 Query: 117 EAAEAAGLSPIKLNVVLMKGINDDEIKDFISLTLNSPLNVRFIEYMPIGSASD-AWRQTY 175 +A AAG + K++ +M+G N DE+ D I VRFIEYM +G A+ AW + + Sbjct: 174 KAVAAAGFTDTKIDTTVMRGANHDELADLIEFARTVNAEVRFIEYMDVGGATHWAWEKVF 233 Query: 176 LPLETVVEACHEAGWATEEADMPSGNGPSQNRRVVGARGTFGLIHPVSEHFCDNCNRLRL 235 ++E+ + E +P + NR + TFG+I +E FC C+R RL Sbjct: 234 TK-ANMLESLEKRYGRIEP--LPKHDTAPANRYALPDGTTFGIIASTTEPFCATCDRSRL 290 Query: 236 TADGHIKACLYWADEYNVR 254 TADG CLY N+R Sbjct: 291 TADGLWLHCLYAISGINLR 309 >Rv3109 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 359 Score = 173 bits (438), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 95/259 (36%), Positives = 150/259 (57%), Gaps = 8/259 (3%) Query: 1 MPAEGMQFQPQDEIMSYEEITAVVKALAPLGLSKIRLTGGEPLVRKDLDKLVSMIAAI-- 58 MP E + P+ +++S +EI+A+V +G+SK+R+TGGEPL+R DL ++V ++A Sbjct: 45 MPEEHYTWLPRQDLLSVKEISAIVDVFLSVGVSKVRITGGEPLIRPDLPEIVRTLSAKVG 104 Query: 59 --PGIEDISLTTNGLMLPAKAALLKQAGLSRVNISLDSLRQDRFAMITRGGEVAKVLKGI 116 G+ D+++TTNG++L + LK AG+ R+ +SLD+L+ +RF I++ KV+ GI Sbjct: 105 EDSGLRDLAITTNGVLLADRVDGLKAAGMKRITVSLDTLQPERFKAISQRNSHDKVIAGI 164 Query: 117 EAAEAAGLSPIKLNVVLMKGINDDEIKDFISLTLNSPLNVRFIEYMPIGSASD-AWRQTY 175 +A AAG + K++ +M+G N DE+ D I VRFIEYM +G A+ AW + + Sbjct: 165 KAVAAAGFTDTKIDTTVMRGANHDELADLIEFARTVNAEVRFIEYMDVGGATHWAWEKVF 224 Query: 176 LPLETVVEACHEAGWATEEADMPSGNGPSQNRRVVGARGTFGLIHPVSEHFCDNCNRLRL 235 ++E+ + E +P + NR + TFG+I +E FC C+R RL Sbjct: 225 TK-ANMLESLEKRYGRIEP--LPKHDTAPANRYALPDGTTFGIIASTTEPFCATCDRSRL 281 Query: 236 TADGHIKACLYWADEYNVR 254 TADG CLY N+R Sbjct: 282 TADGLWLHCLYAISGINLR 300 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,290,005 Number of extensions: 863862 Number of successful extensions: 2552 Number of sequences better than 1.0e-05: 24 Number of HSP's gapped: 2551 Number of HSP's successfully gapped: 24 Length of query: 303 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 197 Effective length of database: 26,153,027 Effective search space: 5152146319 Effective search space used: 5152146319 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)