BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0162.0005.p (299 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1423 [IQR] COG1028 Dehydrogenases with different specificitie... 389 e-108 BH0800 [IQR] COG1028 Dehydrogenases with different specificities... 324 9e-89 BS_ydaD [IQR] COG1028 Dehydrogenases with different specificitie... 294 1e-79 CAC1576 [IQR] COG1028 Dehydrogenases with different specificitie... 283 2e-76 SMa0187 [IQR] COG1028 Dehydrogenases with different specificitie... 247 2e-65 >CAC1423 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 299 Score = 389 bits (998), Expect = e-108, Method: Compositional matrix adjust. Identities = 188/294 (63%), Positives = 222/294 (75%) Query: 6 PYYGERTVCRVQPISFPPQHQDRQPGLESLMHPRPISENPDSRGSGKLDGKVALITGGDS 65 PY+ E C V P +FPPQHQ+RQPG+ES+M PRPI NP SGKL KVALITGGDS Sbjct: 6 PYFRETNNCPVPPTNFPPQHQNRQPGIESVMRPRPIYNNPCYYASGKLRNKVALITGGDS 65 Query: 66 XXXXXXXXXXXXXXXDVAIAYLYEASDAEATQRRIEELGQRCLRIQTDLRCKANCTQAVE 125 DVAI YLYE +DA T+R IE LGQRCL I+ D+R C +AVE Sbjct: 66 GIGRAAAVAFAKEKADVAIVYLYERNDAMETKRVIESLGQRCLTIEGDVRRDGFCREAVE 125 Query: 126 HTVRTFGKLDILVNNHGVQYPQQSLLDISEEQLYETYRTNIFPFFFLTQAALPHLRTGAS 185 T+RTFG L+ILVNN VQ+PQ S+L+I+ EQL T+RTNIF F++T+AALP+L+ G+S Sbjct: 126 RTIRTFGNLNILVNNAAVQFPQDSILNITNEQLENTFRTNIFSMFYITKAALPYLKYGSS 185 Query: 186 IINTASITAYQGHKELIDYSSTKGAIVTFTRSLALSLADSGIRVNSVAPGPVWTPLIPSS 245 IINTASITAY+G K LIDYSSTKGAIV+FTRSL+ SL D GIRVN+VAPGP+WTPLIP+S Sbjct: 186 IINTASITAYEGEKTLIDYSSTKGAIVSFTRSLSKSLVDKGIRVNAVAPGPIWTPLIPAS 245 Query: 246 YSAEEVSVFGTDTPFKRAAQPYELAGAYVYLAGPDSTYVTGTCIHVNGGDMVTS 299 +SA V+ FG DTP KRA QPYELA AYVYLA DS+YVTG IH+NGG MVT+ Sbjct: 246 FSAARVATFGKDTPMKRAGQPYELAPAYVYLASDDSSYVTGEVIHINGGRMVTT 299 >BH0800 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 299 Score = 324 bits (831), Expect = 9e-89, Method: Compositional matrix adjust. Identities = 156/286 (54%), Positives = 201/286 (70%) Query: 14 CRVQPISFPPQHQDRQPGLESLMHPRPISENPDSRGSGKLDGKVALITGGDSXXXXXXXX 73 C P+ FPPQ Q PGLE LM PRPISENP S KL +VA+ITGGDS Sbjct: 14 CDDIPVHFPPQSQPWHPGLEYLMEPRPISENPHRPHSHKLLDRVAIITGGDSGIGRAVAY 73 Query: 74 XXXXXXXDVAIAYLYEASDAEATQRRIEELGQRCLRIQTDLRCKANCTQAVEHTVRTFGK 133 D+ I+YL E DA T++R+EE+G+ CL + DL+ + + VE T+ FGK Sbjct: 74 AFAKEGADLVISYLSEHEDAIETKKRVEEIGRDCLLVPGDLQHEQMSVKVVEKTIERFGK 133 Query: 134 LDILVNNHGVQYPQQSLLDISEEQLYETYRTNIFPFFFLTQAALPHLRTGASIINTASIT 193 LD+LVNNH VQ+ Q SLLDI+EEQL T+RTNI+ +FF+T+AALPHL+ G++IINT S+T Sbjct: 134 LDVLVNNHAVQFRQTSLLDITEEQLDITFRTNIYAYFFMTKAALPHLKKGSAIINTTSVT 193 Query: 194 AYQGHKELIDYSSTKGAIVTFTRSLALSLADSGIRVNSVAPGPVWTPLIPSSYSAEEVSV 253 +Y GH +LIDY++TKGA+ TFTR+L+ SLAD IRVN VAPGP+WTPLIPSSY A + Sbjct: 194 SYLGHSQLIDYAATKGAVTTFTRALSGSLADQEIRVNGVAPGPIWTPLIPSSYPAHSIPT 253 Query: 254 FGTDTPFKRAAQPYELAGAYVYLAGPDSTYVTGTCIHVNGGDMVTS 299 FG+DTP R QP+E+A ++V+LA DS Y+TG +H NGG + S Sbjct: 254 FGSDTPLSRPGQPFEVAPSFVFLASDDSKYLTGQILHPNGGRVTGS 299 >BS_ydaD [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 286 Score = 294 bits (753), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 142/282 (50%), Positives = 191/282 (67%) Query: 18 PISFPPQHQDRQPGLESLMHPRPISENPDSRGSGKLDGKVALITGGDSXXXXXXXXXXXX 77 P P Q Q RQPG+ES M+P P+ E D +G+ KL GKVALITGGDS Sbjct: 5 PKELPAQTQSRQPGIESEMNPSPVYEYEDYKGADKLKGKVALITGGDSGIGRAVSVAYAK 64 Query: 78 XXXDVAIAYLYEASDAEATQRRIEELGQRCLRIQTDLRCKANCTQAVEHTVRTFGKLDIL 137 D+AI Y E DAE T++R+E+ G +CL I D+ + C +AVE TV+ G LDIL Sbjct: 65 EGADIAIVYKDEHEDAEETKKRVEQEGVKCLLIAGDVGEEEFCNEAVEKTVKELGGLDIL 124 Query: 138 VNNHGVQYPQQSLLDISEEQLYETYRTNIFPFFFLTQAALPHLRTGASIINTASITAYQG 197 VNN G Q+P++S+ DI+ EQL+ T++TN + F+LT+ A+ +L+ G++IINT SI Y G Sbjct: 125 VNNAGEQHPKESIKDITSEQLHRTFKTNFYSQFYLTKKAIDYLKPGSAIINTTSINPYVG 184 Query: 198 HKELIDYSSTKGAIVTFTRSLALSLADSGIRVNSVAPGPVWTPLIPSSYSAEEVSVFGTD 257 + LIDY++TKGAI FTR++A +L GIRVN+VAPGP+WTPLIP+++ E V+ FG D Sbjct: 185 NPTLIDYTATKGAINAFTRTMAQALVKDGIRVNAVAPGPIWTPLIPATFPEETVAQFGQD 244 Query: 258 TPFKRAAQPYELAGAYVYLAGPDSTYVTGTCIHVNGGDMVTS 299 TP R QP E G YV LA +S+Y+TG +HVNGG+ VT+ Sbjct: 245 TPMGRPGQPVEHVGCYVLLASDESSYMTGQTLHVNGGNFVTT 286 >CAC1576 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 285 Score = 283 bits (724), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 136/281 (48%), Positives = 190/281 (67%), Gaps = 1/281 (0%) Query: 18 PISFPPQHQDRQPGLESLMHPRPISENPD-SRGSGKLDGKVALITGGDSXXXXXXXXXXX 76 P +FP QHQ+ QPG E M+P PI + ++ G L GK A+ITGGDS Sbjct: 4 PNTFPKQHQNHQPGYEYEMNPAPIYYDEKYNKSRGLLKGKTAIITGGDSGIGRAVSVAYA 63 Query: 77 XXXXDVAIAYLYEASDAEATQRRIEELGQRCLRIQTDLRCKANCTQAVEHTVRTFGKLDI 136 DV I Y E DA+ T++ I+ LG++C + D+ C A+E+T+R + K+DI Sbjct: 64 HEGADVVIIYYDEHQDADETKKIIDGLGRKCTLMPGDIGDTNFCNYAIENTIRAYDKIDI 123 Query: 137 LVNNHGVQYPQQSLLDISEEQLYETYRTNIFPFFFLTQAALPHLRTGASIINTASITAYQ 196 LVNN +QYPQ S+ DIS EQ T++TN++ F++T+AA+ ++ G+SIINT S+ A++ Sbjct: 124 LVNNAAMQYPQNSIADISNEQFDRTFKTNVYGTFYMTRAAISKMKAGSSIINTTSVVAFK 183 Query: 197 GHKELIDYSSTKGAIVTFTRSLALSLADSGIRVNSVAPGPVWTPLIPSSYSAEEVSVFGT 256 G++ LIDYS TKGAI+ FT+SL+ SL SGIRVN+VAPGP+WTPLI +S+ +VS FG+ Sbjct: 184 GNETLIDYSMTKGAILAFTKSLSTSLVKSGIRVNAVAPGPIWTPLISASFDENKVSEFGS 243 Query: 257 DTPFKRAAQPYELAGAYVYLAGPDSTYVTGTCIHVNGGDMV 297 + P +R QP E AGAYV+LA S+Y+TG IHVNGG++V Sbjct: 244 NVPLERPGQPVECAGAYVFLASEMSSYITGQTIHVNGGEIV 284 >SMa0187 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 256 Score = 247 bits (630), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 123/253 (48%), Positives = 169/253 (66%), Gaps = 1/253 (0%) Query: 43 ENPDSRGSGKLDGKVALITGGDSXXXXXXXXXXXXXXXDVAIAYLYEASDAEATQRRIEE 102 + P +GS KL KVALITGGDS DVAI +L E+ DA+ T+ +E+ Sbjct: 3 DAPFYKGSDKLKDKVALITGGDSGIGRSVAVLFAREGADVAIVHLDESQDADDTKAAVEK 62 Query: 103 LGQRCLRIQTDLRCKANCTQAVEHTVRTFGKLDILVNNHGVQYPQQSLLDISEEQLYETY 162 G++CL I+ D++ + C +AVE TV G+LDIL+NN Q + + D+++E ET Sbjct: 63 EGRKCLVIKGDVKDASFCRKAVEKTVMQLGRLDILINNAAFQVHTRDIEDLTDEHFDETL 122 Query: 163 RTNIFPFFFLTQAALPHLRTGASIINTASITAYQGHKELIDYSSTKGAIVTFTRSLALSL 222 +TN++ +F++ +AA+PHL+ G++IINT S+T G KEL+DYS TKG I FTR+L+ L Sbjct: 123 KTNLYGYFYMAKAAIPHLKNGSAIINTGSVTGLTGSKELLDYSMTKGGIHAFTRALSGHL 182 Query: 223 ADSGIRVNSVAPGPVWTPLIPSSYSAEEVSVFGTDTPFKRAAQPYELAGAYVYLAGPD-S 281 GIRVN+VAPGPVWTPL PS AE+V FG+ TP KRAAQP E+A AYV+LA P S Sbjct: 183 VPKGIRVNAVAPGPVWTPLNPSDKEAEDVEKFGSQTPMKRAAQPEEIAPAYVFLASPQMS 242 Query: 282 TYVTGTCIHVNGG 294 +Y+TG + + GG Sbjct: 243 SYITGEILPIVGG 255 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,987,657 Number of extensions: 749576 Number of successful extensions: 2806 Number of sequences better than 1.0e-05: 274 Number of HSP's gapped: 2452 Number of HSP's successfully gapped: 274 Length of query: 299 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 193 Effective length of database: 26,153,027 Effective search space: 5047534211 Effective search space used: 5047534211 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)