BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0044.p (135 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3468 [C] COG0822 NifU homolog involved in Fe-S cluster formation 174 2e-44 CAC3292 [C] COG0822 NifU homolog involved in Fe-S cluster formation 107 4e-24 STM1372 [O] COG0719 ABC-type transport system involved in Fe-S c... 105 2e-23 BH0369 [C] COG0822 NifU homolog involved in Fe-S cluster formation 91 6e-19 AF0565 [C] COG0822 NifU homolog involved in Fe-S cluster formation 79 2e-15 >BH3468 [C] COG0822 NifU homolog involved in Fe-S cluster formation Length = 146 Score = 174 bits (442), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 82/132 (62%), Positives = 103/132 (78%), Gaps = 2/132 (1%) Query: 1 MDHYKNPRNRGSFADDALKIELNNPTCGDRITLQLKVEDGVVKDARYSGEGCSISMSSAS 60 MDHYKNPRNRG+ DD L + +NNPTCGDRI LQ+K++DG V +A++ GEGCSIS++SAS Sbjct: 15 MDHYKNPRNRGTIEDDTLSVNMNNPTCGDRIQLQMKLQDGKVVEAKFIGEGCSISLASAS 74 Query: 61 MMTEAVKGQTVEHALELADRFSSLMKGEAVDFEDYE--DIEALSGVNKFPARIKCATLAW 118 MMT+AVKG V+ AL L+D FS +M G D + ++ DIEAL GV KFPARIKCATLAW Sbjct: 75 MMTQAVKGLKVDEALALSDIFSDMMLGNDYDDKRFDLGDIEALQGVTKFPARIKCATLAW 134 Query: 119 NALRKGIDVEEE 130 AL KG++ +E Sbjct: 135 KALEKGLNEGQE 146 >CAC3292 [C] COG0822 NifU homolog involved in Fe-S cluster formation Length = 143 Score = 107 bits (267), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 52/128 (40%), Positives = 78/128 (60%), Gaps = 3/128 (2%) Query: 2 DHYKNPRNRGSFADDALKIELNNPTCGDRITLQLKVEDGVVKDARYSGEGCSISMSSASM 61 +H + N+ D L +NP+CGD ITLQLK+E+ V++DA + G GC+IS +S S+ Sbjct: 13 EHNASKHNKHKIEDATLVENGHNPSCGDDITLQLKIENNVIEDAAFIGVGCAISQASTSI 72 Query: 62 MTEAVKGQTVEHALELADRFSSLMKGEAVD---FEDYEDIEALSGVNKFPARIKCATLAW 118 M + +KG+TVE A +L + F ++K E D E ED A ++ PAR+KCA LAW Sbjct: 73 MIDLIKGKTVEEAKKLVETFIGMIKREITDEAELEQLEDALAFKNISNMPARVKCAVLAW 132 Query: 119 NALRKGID 126 + + I+ Sbjct: 133 HTFEECIN 140 >STM1372 [O] COG0719 ABC-type transport system involved in Fe-S cluster assembly, permease component Length = 423 Score = 105 bits (261), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 8/125 (6%), Positives = 24/125 (19%) Query: 1 MDHYKNPRNRGSFADDALKIELNNPTCGDRITLQLKVEDGVVKDARYSGEGCSISMSSAS 60 +G + + L T + + + + + ++ Sbjct: 12 QQWQHLFEEKGESRTEQARQHLQQMLRLGLPTRKHEDWKYTPLEGLTHSQFIQQCATISA 71 Query: 61 MMTEAVKGQTVEHALELADRFSSLMKGEAVDFEDYEDIEALSGVNKFPARIKCATLAWNA 120 +A+ Q L D ++ ++ L Sbjct: 72 AQRDALALQIDAVRLVFVDGRFMPELSDSTQNSGFDVSVRDERQALAAPVQPEVFLHLTE 131 Query: 121 LRKGI 125 Sbjct: 132 SLAQC 136 >BH0369 [C] COG0822 NifU homolog involved in Fe-S cluster formation Length = 149 Score = 90.5 bits (223), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 51/127 (40%), Positives = 73/127 (57%), Gaps = 2/127 (1%) Query: 1 MDHYKNPRNRGSFADDALK-IELNNPTCGDRITLQLKVEDGVVKDARYSGEGCSISMSSA 59 +DH K RN + + ++ NPTCGD ITL ++++ V D + GEGCSISM+SA Sbjct: 11 IDHAKKRRNHRRISGEHVQQTHYKNPTCGDVITLYVELQGHHVDDIAFVGEGCSISMASA 70 Query: 60 SMMTEAVKGQTVEHALELADRFSSLMK-GEAVDFEDYEDIEALSGVNKFPARIKCATLAW 118 SMMTE +KG ++E A + +++ GE D D +L GV+K R CA +AW Sbjct: 71 SMMTELLKGVSLEEASSMRHAMERMIRHGEISSDVDLGDALSLEGVHKLRGRHNCALMAW 130 Query: 119 NALRKGI 125 AL + I Sbjct: 131 QALDRVI 137 >AF0565 [C] COG0822 NifU homolog involved in Fe-S cluster formation Length = 153 Score = 79.0 bits (193), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 44/125 (35%), Positives = 72/125 (57%), Gaps = 17/125 (13%) Query: 1 MDHYKNPRNRGSFADDALKIELNNPTCGDRITLQLKVEDGVVKDARYSGEGCSISMSSAS 60 DH++NPRN G D + NP CGD +T+ +KV+D ++D ++ GC+ +++++S Sbjct: 7 FDHFQNPRNVGKIEDADGVGTVGNPVCGDLMTIYIKVKDNRIEDIKFQTFGCAAAIATSS 66 Query: 61 MMTEAVKGQTVEHALELADRFSSLMKGEAVDFEDYEDIEALSGVNKFPARIKCATLAWNA 120 M TE KG+T+E AL++ +AV EAL G+ K ++ C+ LA +A Sbjct: 67 MATEMAKGKTIEEALKITR--------DAV-------AEALGGLPK--QKMHCSNLAADA 109 Query: 121 LRKGI 125 LR+ I Sbjct: 110 LRRAI 114 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.130 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 9,715,945 Number of extensions: 336907 Number of successful extensions: 1249 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 1240 Number of HSP's successfully gapped: 12 Length of query: 135 Length of database: 38,378,431 Length adjustment: 95 Effective length of query: 40 Effective length of database: 27,421,701 Effective search space: 1096868040 Effective search space used: 1096868040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 110 (47.0 bits)