BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0021.p (589 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1125 [V] COG1132 ABC-type multidrug transport system, ATPase a... 552 e-157 BS_yknU [V] COG1132 ABC-type multidrug transport system, ATPase ... 540 e-153 lin0156 [V] COG1132 ABC-type multidrug transport system, ATPase ... 435 e-122 BH0941 [V] COG1132 ABC-type multidrug transport system, ATPase a... 380 e-105 TM0288 [V] COG1132 ABC-type multidrug transport system, ATPase a... 365 e-100 >BH1125 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 581 Score = 552 bits (1422), Expect = e-157, Method: Compositional matrix adjust. Identities = 280/577 (48%), Positives = 387/577 (67%), Gaps = 6/577 (1%) Query: 1 MDVLRQLRGFYREKLHYLILSIFCLAAATAVGLITPNLLRRLIDEVIVPLKFTEXXXXXX 60 MD ++L FY Y S+F + T + ++ P +L+ ID ++ ++ Sbjct: 1 MDTFKRLAQFYWPYRRYFYWSLFAMGVMTLMTVLYPIILQFTIDNAVIGGRYDLIPWLAL 60 Query: 61 XXXXXXXXXXCLQFAHGFFGGRLGNFLAYRLRNACYEKLQFLSFRYYDTAKTGDLMSRLT 120 + + H + G G YRLRN+ YEKLQ+L FRYYD AKTGDLMSRLT Sbjct: 61 ALIIVMGFKGVMVYIHQYLGDLFGVTAVYRLRNSLYEKLQYLPFRYYDNAKTGDLMSRLT 120 Query: 121 GDLEAIRLFIGFGFAQLLNVFFMVLFGSIMMFAINWQLTLITLITMPFLAAVAFRFESKI 180 GD+E R F+ FGFAQ + +V F +M + LTL T+ T+PFLA V +F+ ++ Sbjct: 121 GDVETFRFFLSFGFAQFIQFILLVSFSMGVMLYYSVPLTLATMCTLPFLAIVVAKFDRRV 180 Query: 181 HPAFQEMRLALSSLTTAVQENVTGVRTVKSFAREAYEVEKFSHRNERYKDNQIFAAELWS 240 HP F+++R +L+ L T VQENV+G+ TVK+ ++E EV +F RN Y+ + A +W Sbjct: 181 HPGFRDIRKSLAKLNTKVQENVSGMNTVKALSQEDEEVSRFEDRNVDYRQKHLDIARVWG 240 Query: 241 KFFPVMELLASVSVAILLGVGGTLVIKGHMSLGELVAFFSLIWYIIGPVWGLGFHINNYT 300 +FP+ME + ++S LL GG LVI G M G+LVAFFSL+WYIIGP+ LGF IN ++ Sbjct: 241 NYFPLMEFIGNLSAVFLLSFGGYLVITGSMQTGQLVAFFSLVWYIIGPIMNLGFIINTFS 300 Query: 301 QSKASGERVLEVLNQHIDVKDKENARELVAAEVKGEVVFDHVTFAY-GNKMPAVKDIHFS 359 QSKASGER+LE+L++ V + ++ R ++G V F V Y G + PA++++ F Sbjct: 301 QSKASGERLLEILDEENRVDEGKDER----TSLQGSVTFRDVCLNYRGAETPALQNVSFE 356 Query: 360 APPGAVIGFLGGTGSGKSTIIQLMMRAYDVNKGSIRLDGVDIREYNVRSLRSQIATVFQE 419 AP G IG +G TGSGK++I+QL+ R Y+ G I +DG I Y + +LRSQI V QE Sbjct: 357 APAGKTIGLVGATGSGKTSIVQLVNRFYERTSGEILIDGKPIESYPLETLRSQIGVVLQE 416 Query: 420 TFLFSSSIRNNISYGLKNVGMEEIIRAAKLAKAHDFIMEMPEGYDTVVGERGMGLSGGQK 479 +FLFSS+IR+NI+YG +V M+EII AA+ A+AHDFI E+P+GYDTV+GERG+GLSGGQK Sbjct: 417 SFLFSSTIRDNIAYGRPDVSMKEIIAAARRAQAHDFIAELPDGYDTVLGERGLGLSGGQK 476 Query: 480 QRIAIARALLKNPRILILDDATSAVDMETEHEIQAGFQEVMRGRTTLIIAHRISSLRHAD 539 QRIAIARA+L NP ILILDDATSAVDM+TEH+IQA F+EVM+GRTT IIAHRISS++ AD Sbjct: 477 QRIAIARAILMNPSILILDDATSAVDMKTEHKIQAAFREVMKGRTTFIIAHRISSVKQAD 536 Query: 540 QIIVMNEGNMVQQGTHQELIE-VPGPYQDVYRIQYAD 575 +I+V++ G + ++GTH L++ G Y+ +Y IQ D Sbjct: 537 EILVLDGGKVCERGTHHTLLQNKKGLYRRIYDIQSKD 573 >BS_yknU [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 585 Score = 540 bits (1391), Expect = e-153, Method: Compositional matrix adjust. Identities = 265/576 (46%), Positives = 384/576 (66%), Gaps = 3/576 (0%) Query: 1 MDVLRQLRGFYREKLHYLILSIFCLAAATAVGLITPNLLRRLIDEVIVPLKFTEXXXXXX 60 M+ ++L+ +Y + S+ + TA+ ++ P +L+ IDE+++ ++ Sbjct: 1 METFKRLKMYYWPYRKVFMWSLLAMLLMTAITVVYPIILQITIDEIVLGRQYQLAAWVSL 60 Query: 61 XXXXXXXXXXCLQFAHGFFGGRLGNFLAYRLRNACYEKLQFLSFRYYDTAKTGDLMSRLT 120 F H + G G YRLRN YEKLQ LSF YYD AKTGDLMSRLT Sbjct: 61 GFIAVMVLKGMATFFHQYLGDMFGIKSVYRLRNGLYEKLQRLSFSYYDNAKTGDLMSRLT 120 Query: 121 GDLEAIRLFIGFGFAQLLNVFFMVLFGSIMMFAINWQLTLITLITMPFLAAVAFRFESKI 180 D+E +R F+ +G A+L+ +V +MF + LTL+T+ +PFLA +RF+ ++ Sbjct: 121 ADVEGLRFFLSYGLAELIRFGLLVAISLSVMFYYSVPLTLVTIAVLPFLAVAVYRFDKRV 180 Query: 181 HPAFQEMRLALSSLTTAVQENVTGVRTVKSFAREAYEVEKFSHRNERYKDNQIFAAELWS 240 HPAF+ +R + + L T VQEN++G+ TVKS +RE +++ F+ N Y+ + + +WS Sbjct: 181 HPAFRGIRKSFAKLNTKVQENISGINTVKSLSREDFQISTFNKANAEYRAQYLQTSSIWS 240 Query: 241 KFFPVMELLASVSVAILLGVGGTLVIKGHMSLGELVAFFSLIWYIIGPVWGLGFHINNYT 300 +FP+ME + + + LL GG LV++ ++ GELVAFFSL+ Y++ P+ LGF IN ++ Sbjct: 241 AYFPLMEFIGNTCIVALLSYGGYLVMQNQLNPGELVAFFSLVNYMMWPIMNLGFVINMFS 300 Query: 301 QSKASGERVLEVLNQHIDVKDKENARELVAAEVKGEVVFDHVTFAYGNKMP-AVKDIHFS 359 Q+KASGER+L++L + D+ D +A L ++ G+V F +V+ AYG + A+ ++ F Sbjct: 301 QAKASGERLLDILEKEEDITDHPHA--LHKQKLTGDVHFKNVSLAYGKEQTNALCNVSFE 358 Query: 360 APPGAVIGFLGGTGSGKSTIIQLMMRAYDVNKGSIRLDGVDIREYNVRSLRSQIATVFQE 419 A G VIG LG TGSGKS++ QL+ R Y G I +D I +Y++++LRS I V QE Sbjct: 359 ANSGKVIGLLGPTGSGKSSVTQLLTRFYSPVGGMITIDHKPITDYSLKTLRSNIGVVLQE 418 Query: 420 TFLFSSSIRNNISYGLKNVGMEEIIRAAKLAKAHDFIMEMPEGYDTVVGERGMGLSGGQK 479 +FLFSS+IR+NISYG + ME++I AAK A+AH+FIME+P+GYDT++GERGMGLSGGQK Sbjct: 419 SFLFSSTIRSNISYGRPDASMEDVIEAAKRAQAHNFIMELPDGYDTMLGERGMGLSGGQK 478 Query: 480 QRIAIARALLKNPRILILDDATSAVDMETEHEIQAGFQEVMRGRTTLIIAHRISSLRHAD 539 QRIAIARA+ NP ILILDDATSAVDM+TEH IQ +EVM+ RTT I+AHRISSL+HAD Sbjct: 479 QRIAIARAICLNPSILILDDATSAVDMQTEHSIQLALKEVMKNRTTFIVAHRISSLKHAD 538 Query: 540 QIIVMNEGNMVQQGTHQELIEVPGPYQDVYRIQYAD 575 +I+V N+G + ++GTH EL+E G Y+ +Y +QY D Sbjct: 539 EILVFNKGRIRERGTHHELLEKGGYYKKIYDLQYRD 574 >lin0156 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 580 Score = 435 bits (1119), Expect = e-122, Method: Compositional matrix adjust. Identities = 230/567 (40%), Positives = 348/567 (61%), Gaps = 10/567 (1%) Query: 11 YREKLHYLILSIFCLA-AATAVGLITPNLLRRLIDEVIVPLKFTEXXXXXXXXXXXXXXX 69 Y K L++ +F L A+ + +I P L ++ID+V+ K Sbjct: 10 YVRKYRLLMVGVFILIFIASGISIIYPLLGGKVIDDVVYQNKPNLLIPLLLIMIVSTIIR 69 Query: 70 XCLQFAHGFFGGRLGNFLAYRLRNACYEKLQFLSFRYYDTAKTGDLMSRLTGDLEAIRLF 129 ++++ R+G +++R Y+KLQ L F +++ + GD+M+R+TGD +AIR F Sbjct: 70 TICRYSYQIMCERIGQNSLFQIREDLYKKLQSLDFDFFNNTRVGDIMARMTGDTDAIRHF 129 Query: 130 IGFGFAQLLNVFFMVLFGSIMMFAINWQLTLITLITMPFLAAVAFRFESKIHPAFQEMRL 189 + + +L F+ F I+M I+W+LTL +I P +A + + SK P F E+R Sbjct: 130 VSWVSYNILENVFLFSFAIIIMATIDWKLTLALVIITPLIAILTMKMSSKAQPVFYEIRE 189 Query: 190 ALSSLTTAVQENVTGVRTVKSFAREAYEVEKFSHRNERYKDNQIFAAELWSKFFPVMELL 249 + S L + V+EN++G R VK+FARE +E++KF NE +K + +A++ + PV++ L Sbjct: 190 SFSRLNSMVEENISGNRVVKAFAREDFEMKKFHEHNEDFKKRNLDSADVSRAYLPVLDSL 249 Query: 250 ASVSVAILLGVGGTLVIKGHMSLGELVAFFSLIWYIIGPVWGLGFHINNYTQSKASGERV 309 A V V I L GG LVIKG M+LG+LVAF +W + GP+ G+ IN+ + AS ++ Sbjct: 250 AGVLVVITLIFGGYLVIKGQMTLGDLVAFNGFLWMLNGPMRMSGWLINDVQRFIASSFKI 309 Query: 310 LEVL--NQHIDVKDKENARELVAAEVKGEVVFDHVTFAYGN--KMPAVKDIHFSAPPGAV 365 +++ N I + ++ A L +G V F++V+F + + +K+I A PG Sbjct: 310 QDMMATNAKIPIHAEKPAPSL-----QGHVEFNNVSFHFEDDPNTDVLKNISLKASPGQT 364 Query: 366 IGFLGGTGSGKSTIIQLMMRAYDVNKGSIRLDGVDIREYNVRSLRSQIATVFQETFLFSS 425 + LG TGSGKST++ L+ R YD G I +DGVD R+++VR LR+ IATV Q+ FLFS Sbjct: 365 VAILGETGSGKSTLVNLICRFYDPTSGEILIDGVDARKWHVRELRNHIATVMQDIFLFSD 424 Query: 426 SIRNNISYGLKNVGMEEIIRAAKLAKAHDFIMEMPEGYDTVVGERGMGLSGGQKQRIAIA 485 +I NI++G ME++ + A++A A FI MPE YDT+VGERG+GLSGGQKQRI++A Sbjct: 425 TIEGNIAFGAPEATMEDVRKMARIADADHFIETMPESYDTIVGERGVGLSGGQKQRISLA 484 Query: 486 RALLKNPRILILDDATSAVDMETEHEIQAGFQEVMRGRTTLIIAHRISSLRHADQIIVMN 545 RALLKNP ILILDD TSAVDMETE +IQ +++ TT IIAHRISS++ AD+I+++N Sbjct: 485 RALLKNPSILILDDTTSAVDMETEVKIQGELKKITENTTTFIIAHRISSVKEADEILILN 544 Query: 546 EGNMVQQGTHQELIEVPGPYQDVYRIQ 572 G ++++GTH L+ G Y D+Y Q Sbjct: 545 HGEIIERGTHASLLAEKGYYFDIYNKQ 571 >BH0941 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 584 Score = 380 bits (976), Expect = e-105, Method: Compositional matrix adjust. Identities = 204/505 (40%), Positives = 305/505 (60%), Gaps = 3/505 (0%) Query: 72 LQFAHGFFGGRLGNFLAYRLRNACYEKLQFLSFRYYDTAKTGDLMSRLTGDLEAIRLFIG 131 +++ +F +GN + Y +R+ + +Q LS R+Y K G+++SR+ D+E + F+ Sbjct: 82 IEYYRQYFAQWIGNKILYDIRDHLFTHIQKLSLRFYSNRKVGEIISRVIHDVEQTKTFVM 141 Query: 132 FGFAQLLNVFFMVLFGSIMMFAINWQLTLITLITMPFLAAVAFRFESKIHPAFQEMRLAL 191 G + F ++ +MF++N LTL+ + PF F +K+ + AL Sbjct: 142 TGLMNIWLDMFTIIIALAIMFSMNAWLTLVAIAMFPFYGFSIKYFYAKLRHLTRVRSQAL 201 Query: 192 SSLTTAVQENVTGVRTVKSFAREAYEVEKFSHRNERYKDNQIFAAELWSKFFPVMELLAS 251 + + + E V G+ ++SFA E YE +F+ RN + + I + +K F V+ + Sbjct: 202 ADVQGHLHERVQGMSVIRSFALEDYEQTQFAKRNGTFLNRAIDHTKWNAKTFAVVNTVTD 261 Query: 252 VSVAILLGVGGTLVIKGHMSLGELVAFFSLIWYIIGPVWGLGFHINNYTQSKASGERVLE 311 ++ +++ V VI G + +G + AF + + P+ L TQS AS +RV E Sbjct: 262 IAPLLVIFVAACFVINGELEVGAMTAFVLYMERLYNPLRRLVNSSTTLTQSIASMDRVFE 321 Query: 312 VLNQHIDVKDKENARELVAAEVKGEVVFDHVTFAY-GNKMPAVKDIHFSAPPGAVIGFLG 370 +++ D+ DKE A +L EV G+V FDHV+F+Y + PA+KD+HF G + F+G Sbjct: 322 FIDEKYDIVDKEGAVKL--EEVHGDVTFDHVSFSYEKEETPALKDVHFHVKRGETVAFVG 379 Query: 371 GTGSGKSTIIQLMMRAYDVNKGSIRLDGVDIREYNVRSLRSQIATVFQETFLFSSSIRNN 430 +G GKST+I L+ R YDV+ G I +DG DIR+Y VRSLR QI V QE LFS S+R N Sbjct: 380 MSGGGKSTLISLIPRFYDVSSGRILIDGHDIRDYQVRSLRDQIGMVLQENILFSDSVRMN 439 Query: 431 ISYGLKNVGMEEIIRAAKLAKAHDFIMEMPEGYDTVVGERGMGLSGGQKQRIAIARALLK 490 I G + EE+I AAK A AHDFIM +P GYDT VGERG+ LSGGQKQR+AIAR LK Sbjct: 440 ILMGKPDATEEEVIAAAKAANAHDFIMNLPNGYDTEVGERGVKLSGGQKQRVAIARVFLK 499 Query: 491 NPRILILDDATSAVDMETEHEIQAGFQEVMRGRTTLIIAHRISSLRHADQIIVMNEGNMV 550 NP ILI D+ATSA+D+E+EH IQ +++ + RTT I+AHR+S++ HAD+IIV+ G +V Sbjct: 500 NPPILIFDEATSALDLESEHYIQEAMEKLAKNRTTFIVAHRLSTITHADRIIVVENGEIV 559 Query: 551 QQGTHQELIEVPGPYQDVYRIQYAD 575 + GTH EL+ G Y+ ++ +Q D Sbjct: 560 ESGTHDELMSCNGVYKKLFEVQNLD 584 >TM0288 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 598 Score = 365 bits (936), Expect = e-100, Method: Compositional matrix adjust. Identities = 208/575 (36%), Positives = 322/575 (56%), Gaps = 11/575 (1%) Query: 4 LRQLRGFYREKLHYLILSIFCLAAATAVGLITPNLLRRLIDEVIVPLKFTEXXXXXXXXX 63 LR+L G+ R LI+ + ++ +G+++P L+ + ID V VP +F Sbjct: 25 LRRLLGYLRPHTFTLIMVFVFVTVSSILGVLSPYLIGKTIDVVFVPRRFDLLPRYMLILG 84 Query: 64 XXXXXXXCLQFAHGFFGGRLGNFLAYRLRNACYEKLQFLSFRYYDTAKTGDLMSRLTGDL 123 L + G L + +RLR +EKLQ + ++D GD++SR+ D+ Sbjct: 85 TIYALTSLLFWLQGKIMLTLSQDVVFRLRKELFEKLQRVPVGFFDRTPHGDIISRVINDV 144 Query: 124 EAIRLFIGFGFAQLLNVFFMVLFGSIMMFAINWQLTLITLITMPFLAAVAFRFESKIHPA 183 + I +G Q + + IMMF +N L+L+TL +P + S+ Sbjct: 145 DNINNVLGNSIIQFFSGIVTLAGAVIMMFRVNVILSLVTLSIVPLTVLITQIVSSQTRKY 204 Query: 184 FQEMRLALSSLTTAVQENVTGVRTVKSFAREAYEVEKFSHRNERYKD----NQIFAAELW 239 F E + L L ++E+++G+ +K F RE E+EKF NE + QIF+ L Sbjct: 205 FYENQRVLGQLNGIIEEDISGLTVIKLFTREEKEMEKFDRVNESLRKVGTKAQIFSGVLP 264 Query: 240 SKFFPVMELLASVSVAILLGVGGTLVIKGHMSLGELVAFFSLIWYIIGPVWGLGFHINNY 299 P+M ++ ++ A++ G GG L +K +++G + F P+ L N Sbjct: 265 ----PLMNMVNNLGFALISGFGGWLALKDIITVGTIATFIGYSRQFTRPLNELSNQFNMI 320 Query: 300 TQSKASGERVLEVLNQHIDVKDKENARELVAAEVKGEVVFDHVTFAYGNKMPAVKDIHFS 359 + AS ER+ E+L+ + KD +A EL EV+GE+ F +V F+Y K P +KDI F Sbjct: 321 QMALASAERIFEILDLE-EEKDDPDAVEL--REVRGEIEFKNVWFSYDKKKPVLKDITFH 377 Query: 360 APPGAVIGFLGGTGSGKSTIIQLMMRAYDVNKGSIRLDGVDIREYNVRSLRSQIATVFQE 419 PG + +G TGSGK+TI+ L+MR YDV++G I +DG+DIR+ SLRS I V Q+ Sbjct: 378 IKPGQKVALVGPTGSGKTTIVNLLMRFYDVDRGQILVDGIDIRKIKRSSLRSSIGIVLQD 437 Query: 420 TFLFSSSIRNNISYGLKNVGMEEIIRAAKLAKAHDFIMEMPEGYDTVVGERGMGLSGGQK 479 T LFS++++ N+ YG EEI AAKL + FI +PEGY+TV+ + G LS GQ+ Sbjct: 438 TILFSTTVKENLKYGNPGATDEEIKEAAKLTHSDHFIKHLPEGYETVLTDNGEDLSQGQR 497 Query: 480 QRIAIARALLKNPRILILDDATSAVDMETEHEIQAGFQEVMRGRTTLIIAHRISSLRHAD 539 Q +AI RA L NP+ILILD+ATS VD +TE IQA ++M G+T++IIAHR++++++AD Sbjct: 498 QLLAITRAFLANPKILILDEATSNVDTKTEKSIQAAMWKLMEGKTSIIIAHRLNTIKNAD 557 Query: 540 QIIVMNEGNMVQQGTHQELIEVPGPYQDVYRIQYA 574 IIV+ +G +V+ G H ELI+ G Y +++ QY Sbjct: 558 LIIVLRDGEIVEMGKHDELIQKRGFYYELFTSQYG 592 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.140 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 40,945,878 Number of extensions: 1668366 Number of successful extensions: 12035 Number of sequences better than 1.0e-05: 2007 Number of HSP's gapped: 9309 Number of HSP's successfully gapped: 2171 Length of query: 589 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 477 Effective length of database: 25,461,023 Effective search space: 12144907971 Effective search space used: 12144907971 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 119 (50.4 bits)