BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0010.p (213 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2502 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiestera... 139 2e-33 BH3453 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesteras... 137 1e-32 BS_yunD [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiestera... 129 3e-30 SA0784 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesteras... 125 5e-29 L181858 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiestera... 100 3e-21 >lin2502 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 301 Score = 139 bits (351), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 9/183 (4%), Positives = 31/183 (16%), Gaps = 3/183 (1%) Query: 5 LQRLTLIHTNDIHSHFEMMSPIXXXXXXXXXXXGEE--PVLLLDIGDHMDRAAVETEGTM 62 + I + + + Sbjct: 2 SIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYV 61 Query: 63 GQANIDMINLTGYDAITIGNNEGLTFTREILSALYAGLQCPVVCCNIL-ESATGEAPGWM 121 + M + + E++ + G + +L + G+AP + Sbjct: 62 DTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVIL 121 Query: 122 KRHIILEKDGIKIGITGATAAFASFYGLLGWEALDPEEALREQCELLAPQVDLLIILSHL 181 + + + + L + + + Sbjct: 122 AVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFP 181 Query: 182 GLP 184 Sbjct: 182 EDY 184 >BH3453 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 462 Score = 137 bits (346), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 74/177 (41%), Positives = 102/177 (57%), Gaps = 4/177 (2%) Query: 8 LTLIHTNDIHSHFEMMSPIXXXXXXXXXXXGEEPVLLLDIGDHMDRAAVETEGTMGQANI 67 T+ H ND+HSHF I P LLLD+GDH DR+ V TE TMG+AN+ Sbjct: 6 FTIYHMNDLHSHFTYWPQIVSYMKKEREQTA--PYLLLDLGDHADRSHVITEATMGKANV 63 Query: 68 DMINLTGYDAITIGNNEGLTFTREILSALYAGLQCPVVCCNILESATGEAPGWMKRHII- 126 ++N G D TIGNNEG+TF++E L+ LYA PVV N+ + G+ P W +++ Sbjct: 64 ALLNELGIDYATIGNNEGITFSKEALNELYAQASFPVVVANLFDE-DGKRPSWATPYVLH 122 Query: 127 LEKDGIKIGITGATAAFASFYGLLGWEALDPEEALREQCELLAPQVDLLIILSHLGL 183 + + IG+ G TA F FY LGW+ EE L + E + Q D++++LSHLGL Sbjct: 123 TVSEQLTIGMIGVTAPFTPFYRELGWDVRRHEEVLPQLLEDVRKQADVVVLLSHLGL 179 >BS_yunD [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 462 Score = 129 bits (324), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 71/182 (39%), Positives = 99/182 (54%), Gaps = 6/182 (3%) Query: 6 QRLTLIHTNDIHSHFEMMSPIX---XXXXXXXXXXGEEPVLLLDIGDHMDRAAVETEGTM 62 ++L L HTND+HSHFE I GEE L+ DIGDH+DR TE T Sbjct: 3 EKLRLYHTNDLHSHFENWPKIVDYIEQKRKEHQSDGEE-TLVFDIGDHLDRFQFVTEATF 61 Query: 63 GQANIDMINLTGYDAITIGNNEGLTFTREILSALYAGLQCPVVCCNILESATGEAPGW-M 121 G+AN+D++N D IGNNEG+T E L+ALY + PV+ N+ + G P W + Sbjct: 62 GKANVDLLNRLHIDGAAIGNNEGITLPHEELAALYDHAEFPVIVSNLFDK-NGNRPSWAV 120 Query: 122 KRHIILEKDGIKIGITGATAAFASFYGLLGWEALDPEEALREQCELLAPQVDLLIILSHL 181 HI K+G+ I G T + Y LGW D E+++E + Q D++++LSHL Sbjct: 121 PYHIKSLKNGMSIAFLGVTVPYYPVYDKLGWTVTDALESIKETILEVKGQADIIVLLSHL 180 Query: 182 GL 183 G+ Sbjct: 181 GI 182 >SA0784 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 439 Score = 125 bits (314), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 67/177 (37%), Positives = 98/177 (55%), Gaps = 2/177 (1%) Query: 7 RLTLIHTNDIHSHFEMMSPIXXXXXXXXXXXGEEPVLLLDIGDHMDRAAVETEGTMGQAN 66 RLT+ HTNDIHSH I P L +D+GDH+D +A TE T+G+ N Sbjct: 2 RLTIYHTNDIHSHLHEYERIKVYMAEQRPRLNH-PSLYVDLGDHVDLSAPITEATLGKKN 60 Query: 67 IDMINLTGYDAITIGNNEGLTFTREILSALYAGLQCPVVCCNILESATGEAPGWMKRHII 126 + ++N D TIGNNEG+T + E L+ LY + V C N+++ + G P + I Sbjct: 61 VALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSNVIDES-GHLPNNIVSSYI 119 Query: 127 LEKDGIKIGITGATAAFASFYGLLGWEALDPEEALREQCELLAPQVDLLIILSHLGL 183 + DG+KI ATA F FY L W DP E+++E+ + + D++I+LSH G+ Sbjct: 120 KDMDGVKILFVAATAPFTPFYRALDWIVTDPLESIKEEIKHQRGKFDVIIVLSHCGI 176 >L181858 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 447 Score = 99.8 bits (247), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 66/180 (36%), Positives = 90/180 (50%), Gaps = 13/180 (7%) Query: 5 LQRLTLIHTNDIHSHFEMMSPIXXXXXXXXXXXGEEPVLLLDIGDHMDRAAVETEGTMGQ 64 + +L ++H ND+HSH E I E L D+GD++DR TE T GQ Sbjct: 1 MNKLRILHLNDLHSHLERFPVIERFFAEKSHEEVES--LSFDLGDNVDRVHPLTEATEGQ 58 Query: 65 ANIDMINLTGYDAITIGNNEGLTFTREILSALYAGLQCPVVCCNILESATGEAPGWMKRH 124 N++++N A TIGNNEGL T E+LS LY PV+ N+ + P Sbjct: 59 FNVELMNRLDLTAATIGNNEGLGLTHEMLSHLYDEANFPVLLSNLATNFAQSEPL----- 113 Query: 125 IILEKDGIKIGITGATAAFASFYGLLGWEALDPEEALREQCELLAPQV--DLLIILSHLG 182 I+ G++IG+ G TA + Y GWE DP L E L P++ D I+LSHLG Sbjct: 114 IVTTSFGLRIGLIGLTAPYVLAYPQAGWELSDPFVVLDE----LLPKLDCDFTILLSHLG 169 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.139 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,939,474 Number of extensions: 562015 Number of successful extensions: 925 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 916 Number of HSP's successfully gapped: 15 Length of query: 213 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 111 Effective length of database: 26,614,363 Effective search space: 2954194293 Effective search space used: 2954194293 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 113 (48.1 bits)