BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0009.p (275 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yunD [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiestera... 120 2e-27 BH3453 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesteras... 117 1e-26 SA0784 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesteras... 96 5e-20 lin2502 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiestera... 86 8e-17 BH1015_1 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiester... 71 2e-12 >BS_yunD [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 462 Score = 120 bits (302), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 82/268 (30%), Positives = 133/268 (49%), Gaps = 21/268 (7%) Query: 1 MLETPQMINGTAVCGAGKFGRYIGRLIF-----ERAHAGAAFELVSGRCFTVDPSLTEDV 55 +LE Q++NG + A K+G Y+G + +R+ + + +T + + T+ Sbjct: 206 LLEDGQVVNGVLLASAEKYGHYVGCVEITVDSVQRSINSKTASVQNMAEWTGESAETKAF 265 Query: 56 LAPAAAPHLQHGREVLNETVA--ITDRELTLDTEGESPFGNLLAQAVRRFTGAPISLVNS 113 L + E L++ VA D E+ E E P LLA A++ + IS+VNS Sbjct: 266 LNEKE----REAEEKLSDAVAELAQDAEVKWFEESELPL--LLAYALKEWCETDISMVNS 319 Query: 114 GQLLGPLPQGTITAGMLHALCPSPVNACIIRLKGEDIRKALEQSRMEAFYNKAIFGYGFR 173 G +LGPL G +T LH +CP P+N +RL GE++++ + + E I G GFR Sbjct: 320 GVILGPLKAGPVTKLDLHRICPHPINPVAVRLTGEELKETIVHAASEQMEQLRIKGLGFR 379 Query: 174 GKVLGSLAVDGLKI---LYDPGVLPYDNGNGIAVFVGGEPLDDKAVYSVGTLDMFTFRIG 230 G+V+G + G+++ D G+ + + + GE ++ YSV LDMFT Sbjct: 380 GEVMGKMVYAGVEVETKRLDDGI-----THVTRITLNGEDIEKHKQYSVAVLDMFTLGKL 434 Query: 231 YESLADGTEPLYLLPHFLRDLLRMELQR 258 + + D E Y +P FLRDLL +L + Sbjct: 435 FPLIRDAAEKEYFMPEFLRDLLAWKLAQ 462 >BH3453 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 462 Score = 117 bits (294), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 71/256 (27%), Positives = 132/256 (51%), Gaps = 16/256 (6%) Query: 10 GTAVCGAGKFGRYIGRLIFERAHAGAAFELVSGRCFTVDPSLTEDVLAPAAAPHLQH--- 66 GT + AG+ G+++G++ E + A +V +D + ++ A HL+H Sbjct: 212 GTLITQAGRGGKWLGKV--EVTYDTKARCIVESSAQLID--MEKEDPDQATHLHLEHLNR 267 Query: 67 -GREVLNETVAITDRELTLDTEGESPFGNLLAQAVRRFTGAPISLVNSGQLLGPLPQGTI 125 ++ L++ VA ++LT+D + LL + + GA I ++N+G LL + + Sbjct: 268 EAKQHLSQEVAYLSQQLTVDWFIPTEGAQLLCDGITEWCGADIGMMNAGVLLESIGPNIV 327 Query: 126 TAGMLHALCPSPVNACIIRLKGEDIRKALEQSRMEAFYNKAIFGYGFRGKVLGSLAVDGL 185 T G +H +CP P+N C++++ G+ + + + ++ + G+GFRGKVLG++ G+ Sbjct: 328 TVGDIHRICPHPINPCLVKMTGDALLETIRRAYTREIIELELKGFGFRGKVLGTMLFTGI 387 Query: 186 KILYDP---GVLPYDNGNGIAVFVGGEPLDDKAVYSVGTLDMFTFRIGYESLADGTEPLY 242 I G L V + GEP+ + +Y + TLDM+TF + +A+ E Y Sbjct: 388 DISLSEERDGTLEVTE-----VKIAGEPVVKEQLYELATLDMYTFGYLFPKIAESQEKRY 442 Query: 243 LLPHFLRDLLRMELQR 258 +P LRD+L +L++ Sbjct: 443 FMPEMLRDVLAWQLKK 458 >SA0784 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 439 Score = 96.3 bits (238), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 73/261 (27%), Positives = 128/261 (49%), Gaps = 31/261 (11%) Query: 2 LETPQMINGTAVCGAGKFGRYIGR--LIFERAHAGAAFELVSGRCFTVDPSLTEDVLAPA 59 E ++ NG + AGK+G Y+G L FE AH V + + P T L Sbjct: 201 FEHGEINNGVLMAAAGKYGNYLGEVNLTFE-AHK------VVHKTAKIIPLET---LPEV 250 Query: 60 AAPHLQHGREVLNETV----AITDRELTLDTEGESPFGNLLAQAVRRFTGAPISLVNSGQ 115 + G+ +++ +V + R + TE LLAQ+V +T A +++N+G Sbjct: 251 ETSFEEEGKTLMSNSVIQHPVVLKRSMNHITEA----AYLLAQSVCEYTHAQCAIINAGL 306 Query: 116 LLGPLPQGTITAGMLHALCPSPVNACIIRLKGEDIRKALEQSRMEAFYNKAIFGYGFRGK 175 L+ + + +T +H + P P+N +RL G +++ + +S + + + G GFRG Sbjct: 307 LVKDIVKDEVTEYDIHQMLPHPINMVRVRLSGVKLKEIITKSNKQEYMYEHAQGLGFRGN 366 Query: 176 VLGSLAVDGLKILYDPGVLPYDNGNGIAVFVGGEPLDDKAVYSVGTLDMFTFRIGYESLA 235 + G ILY+ G Y + G ++ GE ++D Y +GT+DM+TF + +L Sbjct: 367 IFGGY------ILYNLG---YIHSTG-RYYLNGEEIEDDKEYVLGTIDMYTFGRYFPTLK 416 Query: 236 DGTEPLYLLPHFLRDLLRMEL 256 + + YL+P FLRD+ + +L Sbjct: 417 ELPKE-YLMPEFLRDIFKEKL 436 >lin2502 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 301 Score = 85.5 bits (210), Expect = 8e-17, Method: Compositional matrix adjust. Identities = 0/267 (0%), Positives = 0/267 (0%), Gaps = 27/267 (10%) Query: 1 MLETPQMINGTAVCGAGKFGRYIGRLIFERAHAGAAFELVSGRCFT-------VDPSLTE 53 E P + + + Sbjct: 207 GAELPYSVTVEIERFVSNERGGYDINGLILVERE---GQKKMVIGNKGAKIKTIGIEARK 263 Query: 54 DVLAPAAAPHLQHGREVLNETVAITDRELTLDTEGESPFGNLLAQAVRRFTGAPISLVNS 113 D+ Sbjct: 264 DMQE----- 318 Query: 114 GQLLGPLPQGTITAGMLHALCPSPVNACIIRLKGEDIRKALE--QSRMEAFYNKAIFGYG 171 Sbjct: 319 378 Query: 172 FRGKVLGSLAVDGLKILYDPGVLPYDNGNGIAVFVGGEPLDDKAVYSVGTLDMFTFRIGY 231 Sbjct: 379 ---------- 428 Query: 232 ESLADGTEPLYLLPHFLRDLLRMELQR 258 Sbjct: 429 455 >BH1015_1 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 707 Score = 70.9 bits (172), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 63/243 (25%), Positives = 104/243 (42%), Gaps = 18/243 (7%) Query: 2 LETPQMINGTAVCGAGKFGRYIGRLIFERAHAGAAFELVSGRCFTVDPSLTEDVLAPAAA 61 L +PQ +NGT + +G +G+++ E V G V D A A Sbjct: 243 LTSPQYVNGTPIVQSGANLTNVGKVVLELDEQTNEVVSVEGSLQAVSELTEVDDEAQAII 302 Query: 62 PHLQHG-REVLNETVAITDRELTLD--TEGESPFGNLLAQAVRRFTGAPISLVNSGQLLG 118 H E+L E + ++D L+ D E ++P GN A+R + A +++ N+G + Sbjct: 303 DHYNSEMDELLGEVIGVSDTGLSRDGRYEADAPLGNFWTDAMRIYAQADVAVTNNGGIRD 362 Query: 119 PLPQGTITAGMLHALCPSPVNACIIRLKGEDIRKALEQSRMEAFYNKAIFGYGFRGKVLG 178 + G +T G ++ + P + + G+ I+ L S + G R ++ Sbjct: 363 SIAPGEVTVGDIYRIEPFANEIMLFEMTGKAIKDVLAYS----------YSRGDRNQI-- 410 Query: 179 SLAVDGLKILYDPGVLPYDNGNGIAVFVGGEPLDDKAVYSVGTLDMF-TFRIGYESLADG 237 L + GL Y+ P N + V V GEP+D++A Y V D T GYE + Sbjct: 411 DLQISGLS--YEIIAGPVGNFIDVRVTVDGEPIDEEATYRVAVPDYIGTGGSGYEFDGEI 468 Query: 238 TEP 240 EP Sbjct: 469 LEP 471 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.141 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,027,655 Number of extensions: 929108 Number of successful extensions: 1583 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 1575 Number of HSP's successfully gapped: 11 Length of query: 275 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 170 Effective length of database: 26,268,361 Effective search space: 4465621370 Effective search space used: 4465621370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)