BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0007.p (362 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0277 [T] COG2206 HD-GYP domain 229 5e-60 CAC0475 [T] COG2206 HD-GYP domain 185 1e-46 RSc2515 [T] COG2206 HD-GYP domain 155 1e-37 RSc0592 [T] COG2206 HD-GYP domain 151 2e-36 BH0008 [T] COG2206 HD-GYP domain 130 3e-30 >CAC0277 [T] COG2206 HD-GYP domain Length = 372 Score = 229 bits (584), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 128/341 (37%), Positives = 205/341 (60%), Gaps = 9/341 (2%) Query: 1 VNRLQAGMKLGKKIYNDEGLVLLADGVELTDALIKRLAKIDIGYIYIEDSVTEDVEITGM 60 + ++ G LGK +Y+ EG VLL G LT A+I ++ I+I IYIED +E + I + Sbjct: 6 IENVKEGSILGKTLYDGEGRVLLTRGSVLTQAIIDKIKSINIYTIYIEDEYSEAI-IEDV 64 Query: 61 LQDETRNQALKVVRSQFQQMSGASG----ITKGFYHLDKKFSK----VMDSILDDLATQE 112 ++ E R A+K+VR+ F +++ + + Y ++ K + I++++ +Q+ Sbjct: 65 IKPEIRRTAIKMVRNTFYRLNKYNNKENYTGRNLYKESEQDFKSIYYIATEIIEEILSQK 124 Query: 113 DPMIMLLDMHTADNYLYVHSLNVCLYTLVLGIAHGYSREELRVIGMGALLHDIGKTQIPV 172 + MI L+D+ + D+Y Y HS+NV + +L++GI + + EL + +GALLHD+GK IP Sbjct: 125 NVMIGLVDIKSFDDYTYQHSVNVAVISLIIGIKLKFHKYELFDLCIGALLHDVGKMFIPS 184 Query: 173 KIIQKPGMLSEEEFRHMQAHTEIGYRILKDEPNIPLLAAHCALQHHERIDGSGYPRGLKG 232 +I+ K G L+EEEF+ M+ HT GY LK I + A +LQHHER G GYP G G Sbjct: 185 EILNKKGELTEEEFKIMKEHTVRGYDYLKGLYEISVPARIISLQHHERAGGQGYPEGRSG 244 Query: 233 PQIHEYAKWLGVADSYDAMTSNRIYKKAMLPHQAVEALYVGSGTLYEQKHLELFRDRVAI 292 +I ++ + VAD YDAMTS+R+Y++A+ P++A+E + SG+ ++ K ++ F V Sbjct: 245 ERISILSRIVAVADVYDAMTSDRVYRRAIAPNEALEYIMANSGSKFDYKVVQAFSKSVIP 304 Query: 293 YPLGLTVKLSTGESGVVVKIDPTIPHRPVVRVLHDPDGEPV 333 YP G V+LS E GVV KI P RP +++++ D V Sbjct: 305 YPEGSIVRLSNNEIGVVTKIHEDFPLRPEIKIIYSEDDSRV 345 >CAC0475 [T] COG2206 HD-GYP domain Length = 351 Score = 185 bits (470), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 107/352 (30%), Positives = 194/352 (55%), Gaps = 14/352 (3%) Query: 4 LQAGMKLGKKIYNDEGLVLLADGVELTDALIKRLAKIDIGYIYIEDSVTEDVEITGMLQD 63 L+A + K IYN+EG +L+ G ++ I L + ++ ++IED D+++ D Sbjct: 9 LKADEVIAKDIYNEEGKLLITAGTKVGRGSISFLKRNNVFLVHIEDEKLSDIKV-----D 63 Query: 64 ETRNQALKVVRSQFQQMSGASGITKGFYHLDKKFSKVMDSILDDLATQEDPMIMLLDMHT 123 + + ++ + + I G Y + + +D ++D + + L ++ Sbjct: 64 KKLEKLKTKTLTKMPLI--FNNILTGEYKNTMEAMETVDELVDYIVDKGTVNTNLYEVKL 121 Query: 124 ADNYLYVHSLNVCLYTLVLGIAHGYSREELRVIGMGALLHDIGKTQIPVKIIQKPGMLSE 183 D+Y Y+H L+ + LG++ G + ++ + + A+LHDIGKT+IP +II K L++ Sbjct: 122 YDDYTYIHCLDTGIMAAFLGLSMGLNTARIKDLSIAAMLHDIGKTKIPSEIINKKEKLTD 181 Query: 184 EEFRHMQAHTEIGYRILKDEPNIPLLAAHC---ALQHHERIDGSGYPRGLKGPQIHEYAK 240 +E+ ++ H G IL +I +L+ HHER DGSGYPRGLKG +I ++ + Sbjct: 182 QEYAIIKNHPAYGRDILN---SIGMLSEEVIDGVFNHHERYDGSGYPRGLKGNEISQFGR 238 Query: 241 WLGVADSYDAMTSNRIYKKAMLPHQAVEALYVGSGTLYEQKHLELFRDRVAIYPLGLTVK 300 + + D + A+++NR Y+ P++A E + GSG++++ ++ FR+ IYPLG V+ Sbjct: 239 IISICDVFTAVSANRSYRLRFKPNEAYELILSGSGSMFDPDFVDEFRNTFFIYPLGSCVR 298 Query: 301 LSTGESGVVVKIDPTIPHRPVVRVLHDPD-GEPVVPFELDLGSKLSVVIVDV 351 LS G G VVK + + P RPV+RV+++ D + P+E+DL ++I +V Sbjct: 299 LSNGVEGYVVKQNKSFPDRPVIRVIYEKDKANSISPYEIDLLQHNDIIIEEV 350 >RSc2515 [T] COG2206 HD-GYP domain Length = 402 Score = 155 bits (392), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 84/246 (34%), Positives = 132/246 (53%), Gaps = 10/246 (4%) Query: 115 MIMLLDMHTADNYLYVHSLNVCLYTLVLGIAHGYSREELRVIGMGALLHDIGKTQIPVKI 174 ++ L + T D Y ++HS+ VC + LG G S +E+R G+ LLHDIGK IP ++ Sbjct: 147 LVSLARLKTKDRYTFLHSVAVCALMVALGRQLGLSDDEVRDAGLAGLLHDIGKIAIPPEV 206 Query: 175 IQKPGMLSEEEFRHMQAHTEIGYRILKDEPNIPLLAAHCALQHHERIDGSGYPRGLKGPQ 234 + KPG L+ +EFRH+ H + GY IL +P++ +A L HHER+DG+GYP L G Sbjct: 207 LNKPGALTTDEFRHVIGHPKAGYDILVGDPSMRDIALDVCLHHHERMDGTGYPHKLAGEN 266 Query: 235 IHEYAKWLGVADSYDAMTSNRIYKKAMLPHQAVEALYVGSGTLYEQKHLELFRDRVAIYP 294 I +A+ + D YDA+TS+R YKKA A++ + G ++ + F V IYP Sbjct: 267 ISLFARMGAICDVYDAVTSDRPYKKAWEAAYAIQRMVEWRGNHFDPMVFQAFVKSVGIYP 326 Query: 295 LGLTVKLSTGESGVVVKIDPTIPHRPVVRVLHDPDGEPVVPFELDLGSKLSVVIVDVTDE 354 G V+L +G VV++ P V+V F + G++++ ++D+ Sbjct: 327 TGSLVRLRSGRLAVVMEHASGALLSPTVKVF----------FSISSGTRITPEVIDLAQT 376 Query: 355 DGAVVK 360 +V Sbjct: 377 GDQIVS 382 >RSc0592 [T] COG2206 HD-GYP domain Length = 403 Score = 151 bits (381), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 85/251 (33%), Positives = 131/251 (52%), Gaps = 1/251 (0%) Query: 100 VMDSILDDLATQEDPMIMLLDMHTADNYLYVHSLNVCLYTLVLGIAHGYSREELRVIGMG 159 ++D++ + LA +I L + D+Y Y+HS VC + L G S +E+R G+G Sbjct: 133 LVDNVSNSLARNSYALIALARLKNKDDYTYLHSFAVCALMVALARTLGLSEDEIRECGLG 192 Query: 160 ALLHDIGKTQIPVKIIQKPGMLSEEEFRHMQAHTEIGYRILKDEPNIPLLAAHCALQHHE 219 L+HDIGK+ +P ++ K L++EE +Q H G+ +L + L HHE Sbjct: 193 GLVHDIGKSAMPRTLLDKSTALTKEELALLQTHAVGGHHLLIGTGQFSEIPREVCLHHHE 252 Query: 220 RIDGSGYPRGLKGPQIHEYAKWLGVADSYDAMTSNRIYKKAMLPHQAVEALYVGSGTLYE 279 RIDGSGYP G KG +I +AK + D YD +TS+ Y A P QA++ + + + ++ Sbjct: 253 RIDGSGYPDGQKGDEISLWAKMGAICDVYDTLTSSSPYHHAWSPAQALKYMMARTDSQFD 312 Query: 280 QKHLELFRDRVAIYPLGLTVKLSTGESGVVVKIDPTIPHRPVVRVLHDPDGEP-VVPFEL 338 + + F V IYP+G VKL T GVVV + + RP V V + + + V P + Sbjct: 313 RTVFQAFTRSVGIYPVGTLVKLRTNRLGVVVHQNESSALRPDVVVFYSGNTKTRVRPERI 372 Query: 339 DLGSKLSVVIV 349 LG +I Sbjct: 373 SLGRSDDAIIT 383 >BH0008 [T] COG2206 HD-GYP domain Length = 360 Score = 130 bits (328), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 71/225 (31%), Positives = 122/225 (54%) Query: 100 VMDSILDDLATQEDPMIMLLDMHTADNYLYVHSLNVCLYTLVLGIAHGYSREELRVIGMG 159 ++ + +++ ++D ++ L T ++YLY H+++V L + LG GY + E I + Sbjct: 114 ILLPLFEEIVEKKDVLLQLHHYSTKEDYLYHHAVSVGLLSAFLGAKLGYEKGEWLKIALA 173 Query: 160 ALLHDIGKTQIPVKIIQKPGMLSEEEFRHMQAHTEIGYRILKDEPNIPLLAAHCALQHHE 219 L D G ++P ++ K G+ + +E M+ H + Y++LK P I LQHHE Sbjct: 174 GALADCGMAKLPTSLLTKNGLYTLKEKHEMRDHPKYSYQMLKKRPEIDGSLLSAVLQHHE 233 Query: 220 RIDGSGYPRGLKGPQIHEYAKWLGVADSYDAMTSNRIYKKAMLPHQAVEALYVGSGTLYE 279 R DG+GYP G++G +IH YA+ L VAD Y AM S R Y+K P+ +E L + Sbjct: 234 REDGTGYPLGVRGQKIHFYAQILAVADVYHAMISERKYRKKKSPYMVLEDLAKERFGKFN 293 Query: 280 QKHLELFRDRVAIYPLGLTVKLSTGESGVVVKIDPTIPHRPVVRV 324 + + + ++ + +V+L+ G +V IDP P RP+V++ Sbjct: 294 PRIVHVLVSQLTQLSVRTSVRLTNNRLGEIVFIDPMAPTRPMVQL 338 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.139 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,846,341 Number of extensions: 1205758 Number of successful extensions: 2024 Number of sequences better than 1.0e-05: 41 Number of HSP's gapped: 1991 Number of HSP's successfully gapped: 41 Length of query: 362 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 254 Effective length of database: 25,922,359 Effective search space: 6584279186 Effective search space used: 6584279186 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)