BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0004.p (453 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1422 [R] COG1253 Hemolysins and related proteins containing C... 349 6e-96 BS_yhdP [R] COG1253 Hemolysins and related proteins containing C... 318 8e-87 BS_yrkA [R] COG1253 Hemolysins and related proteins containing C... 318 1e-86 BS_yugS [R] COG1253 Hemolysins and related proteins containing C... 311 2e-84 BS_yhdT [R] COG1253 Hemolysins and related proteins containing C... 306 4e-83 >CAC1422 [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 428 Score = 349 bits (895), Expect = 6e-96, Method: Compositional matrix adjust. Identities = 182/409 (44%), Positives = 267/409 (65%), Gaps = 6/409 (1%) Query: 25 GIFVAAEFSLVKVRQSRLTQLVSEGNKMAGYALKVNKKLDAYLSATQFGITLASLGLGWV 84 FVAAEFS+VK+R+SR+ LV +G+K A YAL V L++YLSA Q GITLASLGLGWV Sbjct: 18 AFFVAAEFSMVKIRKSRIETLVLDGDKNAKYALGVINNLNSYLSACQLGITLASLGLGWV 77 Query: 85 GEPAISELLVEPLMVQIGVTDHTLISTVSVVVGFSIITFLHIVLGELAPKSLAIQKTEGS 144 GEPA+S++L PL + + +I ++SVVVGF IIT HIVLGELAPKS+AI TE Sbjct: 78 GEPAVSKML-SPLFNLLKIPKQ-IIGSISVVVGFIIITGFHIVLGELAPKSVAILNTESI 135 Query: 145 ALLLSAPLMFFYNVFLPFIWVLNASANALLRLVGVXXXXXXXXXXXXXXIRILMNQSAKS 204 A + PL FY P I N S + +L+L G+ IR+L+ +S K Sbjct: 136 AKATAIPLTLFYKFTYPIIVAFNKSTDLVLKLFGIKQVDEHEEAHTDEEIRMLVEESYKH 195 Query: 205 GVIDKDEMKLMDNIFEFSDLLAREVMLPRTDMDVLYSNLSLEENMRIITETKHSRYPVAF 264 G+IDK E+ +DN+F+FS+ +E+M+PRTDM ++ S ++ + + + +RYPV Sbjct: 196 GLIDKTELTFVDNVFDFSEKTVKEIMVPRTDMKCIFVEDSFDDIVGVTIDEHFTRYPVCE 255 Query: 265 EDKDRIIGFIHITDLLFAPLEQQND-LASLVRPILNVPESIEISHALRLMQKNKAQLTLV 323 E KD IIG IHI DL + ++++ + S++R I VPES+ IS R+ QK + Q+ +V Sbjct: 256 ESKDNIIGLIHIKDLYKLKINKEDEKIESIIREIKFVPESMSISELFRMFQKERMQMAIV 315 Query: 324 VDEYGGTAGLLTAEEILEEIVGDLHDEFEDERPSV--EHNGDYISVEGRMLIEDVNDLTG 381 +DEYGGT GL+T E+ILEEIVG++ DEF++E + NG Y+ V+G++LIED+N+L Sbjct: 316 IDEYGGTFGLVTIEDILEEIVGEIQDEFDEEMEEIIKTENGSYV-VDGKVLIEDINELLD 374 Query: 382 VVIEDDEVDSIGGWLFKELEGNPSKGKRVVVGDVTFEVEEATRLRITRI 430 + I ++ +D+IGGW++ +L+ P +++ G+ F + + R RI +I Sbjct: 375 IEIYEENIDTIGGWIYSKLKKYPKTNEKINYGNYQFIILKCDRRRIEKI 423 >BS_yhdP [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 444 Score = 318 bits (816), Expect = 8e-87, Method: Compositional matrix adjust. Identities = 170/382 (44%), Positives = 251/382 (65%), Gaps = 10/382 (2%) Query: 25 GIFVAAEFSLVKVRQSRLTQLVSEGNKMAGYALKVNKKLDAYLSATQFGITLASLGLGWV 84 FVA+EF+++++R SR+ QL++EGNK A KV LD YLSA Q GITL S+GLG + Sbjct: 17 AFFVASEFAIIRIRGSRIDQLIAEGNKAAIAVKKVTTHLDEYLSACQLGITLTSIGLGVL 76 Query: 85 GEPAISELLVEPLMVQIGVTDHTLISTVSVVVGFSIITFLHIVLGELAPKSLAIQKTEGS 144 GE I LL PL VQ+ V +L +S + ++IITFLH+V+GELAPK++AIQK E Sbjct: 77 GESTIERLL-HPLFVQMNVPG-SLSHVISFIFAYAIITFLHVVVGELAPKTVAIQKAEAV 134 Query: 145 ALLLSAPLMFFYNVFLPFIWVLNASANALLRLVGVXXXXXXXXXXXXXXIRILMNQSAKS 204 ++L + PL++FY + PFIW+LN SA L + G+ +RI++++S KS Sbjct: 135 SMLFAKPLIWFYRIAFPFIWLLNNSARLLTKAFGLETVSENELAHSEEELRIILSESYKS 194 Query: 205 GVIDKDEMKLMDNIFEFSDLLAREVMLPRTDMDVLYSNLSLEENMRIITETKHSRYPVAF 264 G I++ E K ++ IFEF D LA+E+M+PRT++ L ++ + E M II K++RYPV Sbjct: 195 GEINQSEFKYVNKIFEFDDRLAKEIMIPRTEIVSLPHDIKISEMMDIIQIEKYTRYPVEE 254 Query: 265 EDKDRIIGFIHITDLLFAPLEQQ----NDLASLVRPILNVPESIEISHALRLMQKNKAQL 320 DKD IIG I+I ++L A + + + ++ V PI++V ES I L MQK + + Sbjct: 255 GDKDNIIGVINIKEVLTACISGEVSVDSTISQFVNPIIHVIESAPIQDLLVKMQKERVHM 314 Query: 321 TLVVDEYGGTAGLLTAEEILEEIVGDLHDEFE-DERPSVEH--NGDYISVEGRMLIEDVN 377 ++ DEYGGTAGL+T E+I+EEIVG++ DEF+ DE + G YI ++G++LI+ VN Sbjct: 315 AILSDEYGGTAGLVTVEDIIEEIVGEIRDEFDIDEISEIRKIGEGHYI-LDGKVLIDQVN 373 Query: 378 DLTGVVIEDDEVDSIGGWLFKE 399 DL G+ +E++EVD+IGGW + Sbjct: 374 DLLGIHLENEEVDTIGGWFLTQ 395 >BS_yrkA [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 434 Score = 318 bits (816), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 174/419 (41%), Positives = 264/419 (63%), Gaps = 11/419 (2%) Query: 25 GIFVAAEFSLVKVRQSRLTQLVSEGNKMAGYALKVNKKLDAYLSATQFGITLASLGLGWV 84 FVA EF++VK+R S++ QL+ EG K A A KV LD YLSA Q GIT+ +LG+GWV Sbjct: 17 AFFVATEFAIVKIRSSKIDQLILEGKKGAISAKKVITHLDEYLSACQLGITVTALGIGWV 76 Query: 85 GEPAISELLVEPLMVQIGVTDHTLISTVSVVVGFSIITFLHIVLGELAPKSLAIQKTEGS 144 GE E+++ PL V++ T+ + +V+ F + TFLH+V+GELAPK+LAIQK E Sbjct: 77 GESTF-EVILHPLFAHFHVSE-TVSHVLILVIAFVMATFLHVVVGELAPKTLAIQKAETI 134 Query: 145 ALLLSAPLMFFYNVFLPFIWVLNASANALLRLVGVXXXXXXXXXXXXXXIRILMNQSAKS 204 LL + P+++FY + PFIW LN SA ++ L G+ +RIL+++S KS Sbjct: 135 TLLTAKPIIWFYRILFPFIWFLNGSARFIVGLFGLKPASEHELAHSEEELRILLSESYKS 194 Query: 205 GVIDKDEMKLMDNIFEFSDLLAREVMLPRTDMDVLYSNLSLEENMRIITETKHSRYPVAF 264 G I+++E+K ++NIFEF + +A+E+M+PR ++ + S S E ++II ++RYPV Sbjct: 195 GEINQNELKYVNNIFEFDERIAKEIMIPRREIVAISSEDSYETIVKIIKTESYTRYPVLN 254 Query: 265 EDKDRIIGFIHITDLLFA------PLEQQNDLASLVRPILNVPESIEISHALRLMQKNKA 318 DKD IIGFI+ + L A +++ L + + P+++V ES+ I L MQK + Sbjct: 255 GDKDSIIGFINAKEFLSAYIDTDQKIKEDFKLENHINPVIHVIESVPIHDVLVKMQKERT 314 Query: 319 QLTLVVDEYGGTAGLLTAEEILEEIVGDLHDEFE-DERPSVEH-NGDYISVEGRMLIEDV 376 + ++VDEYGGT+GL+TAE+ILEEIVG++ DEF+ DE P++ N ++ ++ ++LIEDV Sbjct: 315 HIAILVDEYGGTSGLVTAEDILEEIVGEIRDEFDKDEVPNIRKVNDNHYILDSKVLIEDV 374 Query: 377 NDLTGVVIEDDEVDSIGGWLFKELEGNPSKGKRVVVGDVTFEVEEATRLRITRINIHRE 435 NDL G + DEVD+IGGW F + + + G + F+V E I + I R+ Sbjct: 375 NDLLGTTLASDEVDTIGGW-FMTQQIDAAVGSVIEADGYIFKVHETVGRHINYLEIVRK 432 >BS_yugS [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 434 Score = 311 bits (797), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 160/378 (42%), Positives = 246/378 (65%), Gaps = 5/378 (1%) Query: 25 GIFVAAEFSLVKVRQSRLTQLVSEGNKMAGYALKVNKKLDAYLSATQFGITLASLGLGWV 84 +FVAAEF++VK+R S++ QL+ G+ A A K+ LD YLSA Q GIT+ +LGLGW+ Sbjct: 22 AVFVAAEFAIVKIRGSKINQLIESGDSRALAAHKIISNLDEYLSACQLGITITALGLGWL 81 Query: 85 GEPAISELLVEPLMVQIGVTDHTLISTVSVVVGFSIITFLHIVLGELAPKSLAIQKTEGS 144 GEP L PL G+ + V+ VV F I+TFLH+V+GELAPK+++IQK E Sbjct: 82 GEPTFERFL-HPLFTMTGIPE-PFNHIVTFVVAFIIVTFLHVVMGELAPKTVSIQKAEAV 139 Query: 145 ALLLSAPLMFFYNVFLPFIWVLNASANALLRLVGVXXXXXXXXXXXXXXIRILMNQSAKS 204 +L ++ PL++FY + PFI LN SA+ L++L G +R+++++S + Sbjct: 140 SLWIAKPLIWFYKITYPFIKALNGSASFLVKLFGFHSVKEHQVVISEEELRLILSESYEK 199 Query: 205 GVIDKDEMKLMDNIFEFSDLLAREVMLPRTDMDVLYSNLSLEENMRIITETKHSRYPVAF 264 G I++ E + ++ IFEF + +ARE+M+PRT++ V+ SLEE + I +++RYPV Sbjct: 200 GEINQSEFRYVNKIFEFDNRVAREIMIPRTEIAVISLEQSLEEAIHHIINERYTRYPVIK 259 Query: 265 EDKDRIIGFIHITDLLFAP-LEQQNDLASLVRPILNVPESIEISHALRLMQKNKAQLTLV 323 +DKD I+G I+ D+ A L Q L ++RP++ V ESI + L MQK + + ++ Sbjct: 260 DDKDHILGIINSKDMFKAYFLGQPIKLNQIMRPVIRVIESIPVQQLLIRMQKERIHMAIL 319 Query: 324 VDEYGGTAGLLTAEEILEEIVGDLHDEF-EDERPSVEHNGDYISV-EGRMLIEDVNDLTG 381 VDEYGGTAGL+T E+I+EEIVG++ DE+ +DE P + G++ V +G+ LI++VNDL Sbjct: 320 VDEYGGTAGLVTVEDIIEEIVGEIRDEYDQDETPHILKKGEHHYVMDGKALIDEVNDLLD 379 Query: 382 VVIEDDEVDSIGGWLFKE 399 + IE++E+D+I GWL + Sbjct: 380 IAIENEEIDTIAGWLLTQ 397 >BS_yhdT [R] COG1253 Hemolysins and related proteins containing CBS domains Length = 80 Score = 306 bits (785), Expect = 4e-83, Method: Compositional matrix adjust. Identities = 0/418 (0%), Positives = 0/418 (0%), Gaps = 12/418 (2%) Query: 27 FVAAEFSLVKVRQSRLTQLVSEGNKMAGYALKVNKKLDAYLSATQFGITLASLGLGWVGE 86 + LV S + + + A + L L + L Sbjct: 20 LLYLAVWLVAAYLSGVAPGFTGFPRWFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDA 79 Query: 87 PAISELLVEPLMVQIGVTDHTLISTVSVVVGFSIITFLHIVLGELAPKSLAIQKTEGSAL 146 Sbjct: 80 -- 137 Query: 147 LLSAPLMFFYNVFLPFIWVLNASANALLRLVGVXXXXXXXXXXXXXXIRILMNQSAKSGV 206 Sbjct: 138 197 Query: 207 IDKDEMKLMDNIFEFSDLLAREVMLPRTDMDVLYSNLSLEENMRIITETKHSRYPVAFED 266 Sbjct: 198 257 Query: 267 KDRIIGFIH----ITDLLFAPLEQQNDLA--SLVRPILNVPESIEISHALRLMQKNKAQL 320 Sbjct: 258 317 Query: 321 TLVVDEYGGTAGLLTAEEILEEIVGDLHDEF-EDERPSVEHNGD-YISVEGRMLIEDVND 378 Sbjct: 318 377 Query: 379 LTGVVIEDDEVDSIGGWLFKELEGNPSKGKRVVVGDVTFEVEEATRLRITRINIHRET 436 Sbjct: 378 -- 433 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,980,333 Number of extensions: 1141455 Number of successful extensions: 2893 Number of sequences better than 1.0e-05: 85 Number of HSP's gapped: 2689 Number of HSP's successfully gapped: 87 Length of query: 453 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 343 Effective length of database: 25,691,691 Effective search space: 8812250013 Effective search space used: 8812250013 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)