BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0156.0008.p (296 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydhR [KG] COG1940 Transcriptional regulator/sugar kinase 315 6e-86 lin0809 [KG] COG1940 Transcriptional regulator/sugar kinase 300 2e-81 CAC1523 [KG] COG1940 Transcriptional regulator/sugar kinase 298 5e-81 L117718 [KG] COG1940 Transcriptional regulator/sugar kinase 231 1e-60 SPy1811 [KG] COG1940 Transcriptional regulator/sugar kinase 224 2e-58 >BS_ydhR [KG] COG1940 Transcriptional regulator/sugar kinase Length = 299 Score = 315 bits (806), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 147/292 (50%), Positives = 198/292 (67%) Query: 2 IIGAIEAGGTKFICGVGNEEGEILDRISFPTETPEETLQQAVAYFKDTGVEAIGIGSFGP 61 ++G IEAGGTKF+C VG E+G I+DRI FPT+ P+ET+++ + YF ++AIGIGSFGP Sbjct: 1 MLGGIEAGGTKFVCAVGREDGTIIDRIEFPTKMPDETIEKVIQYFSQFSLQAIGIGSFGP 60 Query: 62 LNMDFSSPLYGHVTTTPKRGWGGFDFVGYMKRHFPVPVGFDTDVNAAAFGEVKWGAAQGS 121 ++ D +S YG +T TPK GW + F+ +K +PVGF TDVNAAA GE +G A+G Sbjct: 61 VDNDKTSQTYGTITATPKAGWRHYPFLQTVKNEMKIPVGFSTDVNAAALGEFLFGEAKGL 120 Query: 122 DSCXXXXXXXXXXXXXXAEGRLVHGLVHPEGGHVLTRRHAEDVFEGLCPYHGDCLEGLAA 181 DSC EGRL+ GL HPE GH+ RRH +DV++G CPYHGDC EGLA+ Sbjct: 121 DSCLYITIGTGIGAGAIVEGRLLQGLSHPEMGHIYIRRHPDDVYQGKCPYHGDCFEGLAS 180 Query: 182 GPAIEKRWGVKGSALPADHPAWEMEAYYLGQAIAGVVLLLSPKRVILGGGVMHREQLFSL 241 GPAIE RWG K + L WE+E YY+ QA+A +L+L+PK++ILGGGVM ++Q+FS Sbjct: 181 GPAIEARWGKKAADLSDIAQVWELEGYYIAQALAQYILILAPKKIILGGGVMQQKQVFSY 240 Query: 242 IRKEVRQALNGYVAAPEILEAMDTYIVPPGLGDHAGLCGALALGIEALQGPA 293 I + V + +N Y+ E+ + + YIVPP LG +AG+ G L L +ALQ A Sbjct: 241 IYQYVPKIMNSYLDFSELSDDISDYIVPPRLGSNAGIIGTLVLAHQALQAEA 292 >lin0809 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 290 Score = 300 bits (767), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 141/290 (48%), Positives = 190/290 (65%), Gaps = 6/290 (2%) Query: 1 MIIGAIEAGGTKFICGVGNEEGEILDRISFPTETPEETLQQAVAYFKD--TGVEAIGIGS 58 M+ GAIEAGGTKF+ +G + G+I+ R S+PT P ET++ + +FK ++AIGIGS Sbjct: 1 MVYGAIEAGGTKFVVAIGEKSGKIIKRESYPTTEPAETMKAVIQFFKQYQDELKAIGIGS 60 Query: 59 FGPLNMDFSSPLYGHVTTTPKRGWGGFDFVGYMKRHFPVPVGFDTDVNAAAFGEVKWGAA 118 FGP+++ SS YG++T TPK W +D VG MK+ F VP+GF TDVNAAA GEV GAA Sbjct: 61 FGPIDIQKSSATYGYITQTPKLAWRNYDIVGAMKKEFNVPIGFTTDVNAAALGEVSLGAA 120 Query: 119 QGSDSCXXXXXXXXXXXXXXAEGRLVHGLVHPEGGHVLTRRHAEDVFEGLCPYHGDCLEG 178 G DSC G+++ G HPE GH++ RRH D F G CP H DCLEG Sbjct: 121 AGLDSCIYLTIGTGIGGGAVVSGKILEGFSHPEMGHIMVRRHKRDRFTGSCPSHSDCLEG 180 Query: 179 LAAGPAIEKRWGVKGSALPADHPAWEMEAYYLGQAIAGVVLLLSPKRVILGGGVMHREQL 238 LAAG AIEKRWG K + L + W +EA+Y+ QA+ L+LSP+R++LGGGVM + QL Sbjct: 181 LAAGGAIEKRWGQKAAELADNEEVWNLEAHYIAQALMNYTLILSPERIVLGGGVMKQRQL 240 Query: 239 FSLIRKEVRQALNGYVAAPEILEAMDTYIVPPGLGDHAGLCGALALGIEA 288 F L+R++++ +N YV P+ +D YIVPP L D AG+ G + L ++A Sbjct: 241 FPLVRQKLKALVNNYVQLPD----LDEYIVPPKLEDDAGITGCVLLAVDA 286 >CAC1523 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 288 Score = 298 bits (764), Expect = 5e-81, Method: Compositional matrix adjust. Identities = 142/288 (49%), Positives = 189/288 (65%), Gaps = 4/288 (1%) Query: 1 MIIGAIEAGGTKFICGVGNEEGEILDRISFPTETPEETLQQAVAYFKDTGVEAIGIGSFG 60 M+ GAIEAGGTKF+C V +E I++R S T PEET+ +F +EAIGIGSFG Sbjct: 1 MMYGAIEAGGTKFVCAVSDENLNIIERESIKTTIPEETMTLVFKFFDKFKLEAIGIGSFG 60 Query: 61 PLNMDFSSPLYGHVTTTPKRGWGGFDFVGYMKRHFPVPVGFDTDVNAAAFGEVKWGAAQG 120 P++++ S YG++T TPK+GW +DFVG +K + +P+G+ TDVNAAA GE+K GAA Sbjct: 61 PIDVNKKSKTYGYITNTPKQGWANYDFVGAVKNRYNIPIGWTTDVNAAALGELKKGAAVN 120 Query: 121 SDSCXXXXXXXXXXXXXXAEGRLVHGLVHPEGGHVLTRRHAEDVFEGLCPYHGDCLEGLA 180 +SC +G+L+ G HPE GH+L RRH +D +EG CP+H DCLEG+A Sbjct: 121 LESCVYLTVGTGIGGGAVVKGKLLEGYGHPEMGHILVRRHEKDTYEGKCPFHKDCLEGMA 180 Query: 181 AGPAIEKRWGVKGSALPADHPAWEMEAYYLGQAIAGVVLLLSPKRVILGGGVMHREQLFS 240 AGPAIE RWG K L + WEMEAYY+ Q + L LSP+R++LGGGVM + QLF Sbjct: 181 AGPAIEARWGKKAYDLTDKNEVWEMEAYYIAQGLMTYTLTLSPERIVLGGGVMKQLQLFP 240 Query: 241 LIRKEVRQALNGYVAAPEILEAMDTYIVPPGLGDHAGLCGALALGIEA 288 LIRK + + + YVA P + E YIVPP L D+AG+ G L L +++ Sbjct: 241 LIRKHLEKLMANYVAMPNLEE----YIVPPALKDNAGITGCLLLAMDS 284 >L117718 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 291 Score = 231 bits (589), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 108/283 (38%), Positives = 171/283 (60%), Gaps = 8/283 (2%) Query: 4 GAIEAGGTKFICGVGNEEGEILDRISFPTETPEETLQQAVAYFKDTGVEAIGIGSFGPLN 63 G+IEAGGTKF+ +G+E+ IL PT +PEET+ + + +FK+ V+++ +GSFGP++ Sbjct: 6 GSIEAGGTKFVLAIGDEQFNILKTNQIPTTSPEETITRTIEFFKENPVKSLAVGSFGPID 65 Query: 64 MDFSSPLYGHVTTTPKRGWGGFDFVGYMKRHFPVPVGFDTDVNAAAFGEVKWGAAQGSDS 123 + S YG++TTTPK GW D +G +K +P+ F TDVN++A+GE+ G S Sbjct: 66 IKPESATYGYITTTPKAGWANVDLLGKLKAALDIPMVFTTDVNSSAYGEM---IVTGGKS 122 Query: 124 CXXXXXXXXXXXXXXAEGRLVHGLVHPEGGHVLTRRHAEDV-FEGLCPYHGDCLEGLAAG 182 +G+ + G H E GH +RH +D+ F G+CP+HGDCLEG+AAG Sbjct: 123 LVYFTIGTGIGGGAVQDGKFIGGFSHAEMGHQYVKRHPDDLNFAGVCPFHGDCLEGVAAG 182 Query: 183 PAIEKRWGVKGSALPADHPAWEMEAYYLGQAIAGVVLLLSPKRVILGGGVMHREQLFSLI 242 P++E R G++G +PAD W+++A+Y+ Q L L+P++++ GGGVM + + + Sbjct: 183 PSLEARLGIRGEDIPADSKVWDIQAFYIAQIAVNTTLALAPEKIVFGGGVMTQTHMIERV 242 Query: 243 RKEVRQALNGYVAAPEILEAMDTYIVPPGLGDH-AGLCGALAL 284 RKE + + GYV P +L YI+ P + ++ + G AL Sbjct: 243 RKEFTKLMAGYVHVPYLLT---DYIMTPSVANNGSATIGNFAL 282 >SPy1811 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 294 Score = 224 bits (570), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 117/296 (39%), Positives = 172/296 (58%), Gaps = 12/296 (4%) Query: 2 IIGAIEAGGTKFICGVGNEEGEILDRISFPTETPEETLQQAVAYFK--DTGVEAIGIGSF 59 + G+IEAGGTKF+C VG+EE ++D+ FPT TPEET+ + +AYFK + + + IGSF Sbjct: 4 LYGSIEAGGTKFVCAVGDEEFTVVDKTQFPTTTPEETIARTIAYFKAFEADLAGMAIGSF 63 Query: 60 GPLNMDFSSPLYGHVTTTPKRGWGGFDFVGYMKRHFPVPVGFDTDVNAAAFGEVKWGAAQ 119 GP+++D SS YG++TTTPK GW D +G + F +P TDVN++A+GEV A Sbjct: 64 GPIDIDPSSETYGYITTTPKSGWANVDLLGQLSAAFKIPFDVTTDVNSSAYGEVL--ARP 121 Query: 120 GSDSCXXXXXXXXXXXXXXAEGRLVHGLVHPEGGHVLTRRHAEDV---FEGLCPYHGDCL 176 G +S G + GL H E GH H +D+ F G+CP+H CL Sbjct: 122 GVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVMPHPDDMAKGFLGVCPFHKGCL 181 Query: 177 EGLAAGPAIEKRWGVKGSALPADHPAWEMEAYYLGQAIAGVVLLLSPKRVILGGGVMHRE 236 EG+AAGP+IE R GV+G L + W+++A+Y+ QA +L P+ ++ GGGVM +E Sbjct: 182 EGMAAGPSIEARTGVRGERLDQEADVWDIQAFYIAQAALQATMLYRPQVIVFGGGVMAQE 241 Query: 237 QLFSLIRKEVRQALNGYVAAPEILEAMDTYIVPPGLGDH-AGLCGALALGIEALQG 291 + + + L+GY+ P++ + YIV P + D+ + G AL A QG Sbjct: 242 HMVLRVHDKFTALLSGYLPVPDLTD----YIVTPAVADNGSATLGNFALAKLAAQG 293 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.141 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,832,624 Number of extensions: 1047377 Number of successful extensions: 1977 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 1967 Number of HSP's successfully gapped: 15 Length of query: 296 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 190 Effective length of database: 26,153,027 Effective search space: 4969075130 Effective search space used: 4969075130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)