BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0156.0004.p (291 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yurN [G] COG1175 ABC-type sugar transport systems, permease c... 148 1e-35 lin0760 [G] COG1175 ABC-type sugar transport systems, permease c... 139 6e-33 AGc4553 [G] COG1175 ABC-type sugar transport systems, permease c... 137 1e-32 TM0431 [G] COG1175 ABC-type sugar transport systems, permease co... 135 1e-31 BS_yesP [G] COG1175 ABC-type sugar transport systems, permease c... 133 4e-31 >BS_yurN [G] COG1175 ABC-type sugar transport systems, permease components Length = 292 Score = 148 bits (373), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 92/284 (32%), Positives = 152/284 (53%), Gaps = 7/284 (2%) Query: 12 VFILPALIFYLGTVFVPIIQSLKYGFQQWNGIQ-EPHYIGLDNYIAMFKDSYFWNSXXXX 70 +F++PAL+F L V++PI +++ QW+ E +IGL NY+ +F D F+ + Sbjct: 11 LFLVPALVFLL-FVYIPIFENVFLSLFQWSSFSPEKTFIGLKNYVELFHDPVFYQALTNN 69 Query: 71 XXXXXXXXXXXXXXGLFFALMLS--YVTKGAGIFRTLYYLPAVVVTVAIAQMFRNFYSLQ 128 GL A +L V K + FRT+++LP V+ IA +F Y+ + Sbjct: 70 VLYAVISIVCQVFGGLILAAVLEDKLVRKWSPFFRTVFFLPVVISMTVIALLFDFIYNPE 129 Query: 129 PLGLLNMFLDWVGLSHLQSAWLSNPHTALIAVSIPEGWRFIGMYMVIFYAALIAIPKEIE 188 GLLN L +GL L AWL + TA+++V W+ +G +++ ++ IP E+ Sbjct: 130 T-GLLNQLLQAIGLDQLTRAWLGDDSTAMLSVIFVSQWQSVGYIAMLYIVSIQKIPDELY 188 Query: 189 EAATIDGVSGWQLIRYIKLPSIMHVLSLSLIMCTTGALRGFDIPYII--GVPGSATELVT 246 EAA +DG Q +I +P + ++++M TGA F+ PYI+ G PG A+E+++ Sbjct: 189 EAARLDGAGKIQQFFHITVPQTKEMSFVAVVMTLTGAFTVFNEPYILTGGGPGKASEVLS 248 Query: 247 TYMYKQAFSTIQYGYGSAIAVFIVIESLLAVLIIRAIFNSRKGD 290 T++YK AF+ GY SAIA ++I +L L+ F + K + Sbjct: 249 TFLYKSAFTKDMMGYASAIATVVLIITLALSLMQMKFFKTGKEE 292 >lin0760 [G] COG1175 ABC-type sugar transport systems, permease components Length = 296 Score = 139 bits (350), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 93/290 (32%), Positives = 148/290 (51%), Gaps = 9/290 (3%) Query: 5 KDKKAWLVFILPALIFYLGTVFVPIIQSLKYGFQQWNGIQEPHYIGLDNYIAMFKDSYFW 64 + + A+L LP L+ + + +PI +L F ++ + P++IG DNYI MF+D YF Sbjct: 13 QQRMAYLFIALPVLLLAIFMI-LPIGMALFVSFTDYDIVNSPNWIGFDNYIKMFRDPYFL 71 Query: 65 NSXXXXXXXXXXXXXXXXXXGLFFALMLSYVTKGAGIFRTLYYLPAVVVTVAIAQMFRNF 124 S L A++++ KGAG+FRT +Y+P + TVA A ++ + Sbjct: 72 ISLKNTIIYTALFVPLGLVASLGAAILINMNKKGAGLFRTFFYVPVLCSTVATATIW--Y 129 Query: 125 YSLQP-LGLLNMFLDWVGLSHLQSAWLSNPHTALIAVSIPEGWRFIGMYMVIFYAALIAI 183 + L P GL+N L W G++ AWL + + A+ A+ + W G M+IF A L I Sbjct: 130 WLLNPQYGLINRVLGWFGING--PAWLYDSNWAMFAIVMMSVWMTFGTNMMIFLAGLQGI 187 Query: 184 PKEIEEAATIDGVSGWQLIRYIKLPSIMHVLSLSLIMCTTGALRGFDIPYII--GVPGSA 241 P + EA+ I+G S W+ RY+ PS+ L M A + FD Y++ G PG++ Sbjct: 188 PANLYEASKIEGASRWKTFRYVTWPSLSRTTFLVTTMLIINAFQVFDQAYVLTKGGPGNS 247 Query: 242 TELVTTYMYKQAFSTIQYGYGSAIAVFIVIESLLAVLIIRAIFNSRKGDA 291 T + Y+Y + F ++ GY SAI+ F++ +L II N A Sbjct: 248 TITLVYYIYNEGFGGLKMGYASAIS-FVLFLIILVFSIINMRVNKEDRTA 296 >AGc4553 [G] COG1175 ABC-type sugar transport systems, permease components Length = 312 Score = 137 bits (346), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 88/272 (32%), Positives = 142/272 (52%), Gaps = 7/272 (2%) Query: 13 FILPALIFYLGTVFVPIIQSLKYGFQQWNGIQEPHYIGLDNYIAMFKDSYFWNSXXXXXX 72 F++PAL+ + P + +L +W QE +IG DNYI + D FW S Sbjct: 31 FVIPALVVISAVIVFPWVFTLWMSVHRWTLGQEQSFIGFDNYIRLASDLRFWESLWHTLI 90 Query: 73 XXXXXXXXXXXXGLFFALMLSYVTKGAGIFRTLYYLPAVVVTVAIAQMFRNFYSLQPLGL 132 G AL+ G R ++ +P + VAIA ++ + Q LG+ Sbjct: 91 YTVLSVVAPLFLGTLAALVFDAQFPLRGFLRGVFVMPMMATPVAIALVWTMMFHPQ-LGV 149 Query: 133 LNMFLDWVGLSHLQSAWLSNPHTALIAVSIPEGWRFIGMYMVIFYAALIAIPKEIEEAAT 192 LN L +G+ L+ W+ N T + ++ + E W++ + M+I L A+P+E E+A Sbjct: 150 LNYLLSLIGIGPLE--WIYNQSTVIPSLVLVETWQWTPLVMLIVLGGLAAVPREPYESAE 207 Query: 193 IDGVSGWQLIRYIKLPSIMHVLSLSLIMCTTGALRGFDIPYII--GVPGSATELVTTYMY 250 IDG + WQ RY+ +P I L +++I+ + A++ FDI Y + G PG+A+E + Y+Y Sbjct: 208 IDGANAWQKFRYLTMPMIAPFLMIAVIIRSIDAVKSFDIIYAMTQGGPGTASETINIYLY 267 Query: 251 KQAFSTIQYGYGSAIAV--FIVIESLLAVLII 280 AFS GYGSA+AV F++I +L VL++ Sbjct: 268 NTAFSYYDIGYGSAMAVVFFVIIVALSFVLLM 299 >TM0431 [G] COG1175 ABC-type sugar transport systems, permease components Length = 286 Score = 135 bits (339), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 87/283 (30%), Positives = 139/283 (49%), Gaps = 5/283 (1%) Query: 8 KAWLVFILPALIFYLGTVFVPIIQSLKYGFQQWNGIQEPHYIGLDNYIAMFKDSYFWNSX 67 ++ +F+LP L+F + V VP+I GF +WN + P ++GL+N+ + KD +FWNS Sbjct: 7 RSRFIFVLPGLVFLVLFVAVPVISVFIMGFFKWNLLSPPRFVGLENFKMLLKDKWFWNSV 66 Query: 68 XXXXXXXXXXXXXXXXXGLFFALMLSYVTKGAGIFRTLYYLPAVVVTVAIAQMFRNFYSL 127 L AL L T + I R +Y P ++ VA M++ S Sbjct: 67 WVSIKLLLLAVPMQFFVSLGLALCLYEETTASKILRAFFYWPYMMPAVAGTTMWKWLLSY 126 Query: 128 QPLGLLNMFLDWVGLSHLQSAWLSNPHTALIAVSIPEGWRFIGMYMVIFYAALIAIPKEI 187 +GLLN L +GL + WL P AL ++++ W G+ MV+F L +P++ Sbjct: 127 D-IGLLNHILRTLGLPPVP--WLIKPFPALFSIALLRTWGMTGLLMVMFITGLQNVPQDY 183 Query: 188 EEAATIDGVSGWQLIRYIKLPSIMHVLSLSLIMCTTGALRGFDIPYII--GVPGSATELV 245 EAA IDG + WQ Y+ P++ + L L+ LR F Y++ G PG AT ++ Sbjct: 184 IEAAMIDGANRWQRFWYVVFPAMSNTNLLVLMTAIAHTLRSFAGVYVLTTGGPGYATTII 243 Query: 246 TTYMYKQAFSTIQYGYGSAIAVFIVIESLLAVLIIRAIFNSRK 288 Y+Y+ AF+ + GY A +V + + +I + SR Sbjct: 244 PLYIYQTAFTQFRIGYSYAASVIYFLIAFAIAMITLKLRESRS 286 >BS_yesP [G] COG1175 ABC-type sugar transport systems, permease components Length = 309 Score = 133 bits (334), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 96/288 (33%), Positives = 150/288 (52%), Gaps = 11/288 (3%) Query: 2 NVLKDKKAWLVFILPALIFYLGTVFVPIIQSLKYGFQQWNGIQEPHYIGLDNYIAMFK-D 60 ++ KD A FI P +I +L +P+ SL F ++ P +IGLDN+ MF D Sbjct: 14 SIRKDNLAGYAFISPFIIGFLCFTVIPMGASLFLSFTSYDLFTAPKWIGLDNFKEMFTGD 73 Query: 61 SYFWNSXXXXXXXXXXXXXXXXXXGLFFALMLSYVTKGAGIFRTLYYLPAVVV-TVAIAQ 119 +W S LF A++L+ KG I+RTL+YLP+++ +VA+A Sbjct: 74 EKYWQSLKVTFTYVLAGVPLRLGFALFIAVILNNAAKGTAIYRTLFYLPSIIGGSVAVAI 133 Query: 120 MFRNFYSLQPLGLLNMFLDWVGLSHLQSAWLSNPHTALIAVSIPEGWRFIGMYMVIFYAA 179 M+RN + G++N L +VG+ + W NP +AL + + W+F G M+IF A Sbjct: 134 MWRNIFGND--GVINALLFFVGIDQ-KILWYQNPTSALWTLILLSVWQF-GSSMLIFLAG 189 Query: 180 LIAIPKEIEEAATIDGVSGWQLIRYIKLPSIMHVLSLSLIMCTTGALRGFDIPYII---- 235 L IP EAA++DG + Q I LP + ++ +L+M T A F YII Sbjct: 190 LKNIPSSYLEAASVDGANRVQRFFKITLPILTPIIFFNLVMQTISAFMTFTPAYIISKGE 249 Query: 236 GVPGSATELVTTYMYKQAFSTIQYGYGSAIA-VFIVIESLLAVLIIRA 282 G P T L + Y++++AF+ Q GY SA+A V +VI L+ +++ + Sbjct: 250 GGPLDGTLLYSLYLFQRAFNYFQMGYASAMAWVMLVIVGLITLILFKT 297 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.143 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,979,124 Number of extensions: 747839 Number of successful extensions: 2716 Number of sequences better than 1.0e-05: 160 Number of HSP's gapped: 2488 Number of HSP's successfully gapped: 161 Length of query: 291 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 186 Effective length of database: 26,268,361 Effective search space: 4885915146 Effective search space used: 4885915146 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)