BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0042.p (313 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF0620 [M] COG0463 Glycosyltransferases involved in cell wall bi... 98 2e-20 SMb20958 [M] COG0463 Glycosyltransferases involved in cell wall ... 71 3e-12 Rv0696 [M] COG0463 Glycosyltransferases involved in cell wall bi... 65 2e-10 MT0723 [M] COG0463 Glycosyltransferases involved in cell wall bi... 65 2e-10 NMB1929 [M] COG0463 Glycosyltransferases involved in cell wall b... 56 8e-08 >AF0620 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 297 Score = 97.8 bits (242), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 71/189 (37%), Positives = 104/189 (55%), Gaps = 13/189 (6%) Query: 70 DIRYILEPVQGLSAARNTGILASKSPLIAFLDDDAIPCRNWITTIVSTFEQKPEVMAMGG 129 D+R ++ GLS ARN G+ +K +IAF+DDDA W+ IV + + V +GG Sbjct: 56 DVRVVVSDGFGLSKARNKGVFEAKGDIIAFIDDDAWADDLWLEKIVENYSDEG-VYGVGG 114 Query: 130 KIAPIFESRRPDWLIKPFELPYTIVDLGNRVKEYPKR----LHPCGANMAMRKVAFDIS- 184 KI P+FE+ RP WL P EL + + G K P++ +P GANM+ R+ AF+I+ Sbjct: 115 KIIPVFENGRPKWL--PEELDWIV---GCTYKGMPEQRTNIRNPIGANMSFRREAFEIAG 169 Query: 185 LFPLELGRKGDSLLSGEETWLFNQIE--SQGHSILYHPQMAVDHFVPANRLTEDWIMKRY 242 F E+GR G LLSGEE ++ I+Y P V H VP +R+ + +KR Sbjct: 170 PFRSEIGRYGKKLLSGEEAEFAMRLRKIKPDAKIIYEPSAVVYHKVPKSRVRISYAVKRA 229 Query: 243 YSQGISNAM 251 + +G S A+ Sbjct: 230 FYEGYSKAI 238 >SMb20958 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 301 Score = 70.9 bits (172), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 16/291 (5%), Positives = 42/291 (14%), Gaps = 54/291 (18%) Query: 12 ISIIICTYNR-SAXXXXXXXXXXXXENLDQAEVIVVDNSSKDDTAAVVKRFIEAEGAN-- 68 I ++ + + A Sbjct: 8 CGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGA------YQAIYV 61 Query: 69 --MDIRYILEPVQGLSAARNTGILASKSPLIAFLDDDAIPCRNWITTIVSTFEQKPEVMA 126 + + + L+ F+ + + + E K V+ Sbjct: 62 DTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVIL 121 Query: 127 MG------GKIAPI------FESRRPDWLIKPFELPYTIVDLGNRVKEYPKRLHPCGANM 174 + A + S+ I P + Sbjct: 122 AVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETG-----------------LNVDT 164 Query: 175 AMRKVAFDISLFPLELGRKGDSLLSGEETWLFNQIESQGHSILYHPQMAVDHFVPANRLT 234 V + + L A + + Sbjct: 165 IAAIVRKHLPEATHHFPEDY-----ITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIE 219 Query: 235 EDWIMKRYYSQGISN----AMKSEGVKG--NLLLLGKTAAKIMYIAGDSIL 279 +R + + + + D Sbjct: 220 RFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIK---TIGIEARKDMQE 267 >Rv0696 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 470 Score = 64.7 bits (156), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 61/219 (27%), Positives = 98/219 (44%), Gaps = 31/219 (14%) Query: 37 NLDQAEVIVVDNSSKDDTAAVVKRFIEAEGANMDIRYILEP-VQGLSAARNTGILASKSP 95 +L VIVVD+ S + F+ GA+ DI + P +G +AARNTG+ A + Sbjct: 103 SLRGLRVIVVDDGSACPVES--DDFV---GAHCDIEVLHHPHSKGPAAARNTGLAACTTD 157 Query: 96 LIAFLDDDAIPCRNWITTIVSTFEQKPEVMAMGGKIAPIFESRRPDWLIKPFELPYTIVD 155 +AFLD D P R W+ +++ F P V + +I + E P + +E ++ +D Sbjct: 158 FVAFLDSDVTPRRGWLESLLGHF-CDPTVALVAPRIVSLVEGENP---VARYEALHSSLD 213 Query: 156 LGNRVKEYPKRLH------PCGANMAMRKVAFDISLFPLELGRKGDSLLSGEETWLFNQI 209 LG R E P H P A + D+ F +++ SGE+ L ++ Sbjct: 214 LGQR--EAPVLPHSTVSYVPSAAIVCRSSAIRDVGGF-------DETMHSGEDVDLCWRL 264 Query: 210 ESQGHSILYHPQMAVDHFVPANRLTE--DWIMKRYYSQG 246 G + Y P V H + T+ DWI ++ + G Sbjct: 265 IEAGARLRYEPIALVAH----DHRTQLRDWIARKAFYGG 299 >MT0723 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 470 Score = 64.7 bits (156), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 61/219 (27%), Positives = 98/219 (44%), Gaps = 31/219 (14%) Query: 37 NLDQAEVIVVDNSSKDDTAAVVKRFIEAEGANMDIRYILEP-VQGLSAARNTGILASKSP 95 +L VIVVD+ S + F+ GA+ DI + P +G +AARNTG+ A + Sbjct: 103 SLRGLRVIVVDDGSACPVES--DDFV---GAHCDIEVLHHPHSKGPAAARNTGLAACTTD 157 Query: 96 LIAFLDDDAIPCRNWITTIVSTFEQKPEVMAMGGKIAPIFESRRPDWLIKPFELPYTIVD 155 +AFLD D P R W+ +++ F P V + +I + E P + +E ++ +D Sbjct: 158 FVAFLDSDVTPRRGWLESLLGHF-CDPTVALVAPRIVSLVEGENP---VARYEALHSSLD 213 Query: 156 LGNRVKEYPKRLH------PCGANMAMRKVAFDISLFPLELGRKGDSLLSGEETWLFNQI 209 LG R E P H P A + D+ F +++ SGE+ L ++ Sbjct: 214 LGQR--EAPVLPHSTVSYVPSAAIVCRSSAIRDVGGF-------DETMHSGEDVDLCWRL 264 Query: 210 ESQGHSILYHPQMAVDHFVPANRLTE--DWIMKRYYSQG 246 G + Y P V H + T+ DWI ++ + G Sbjct: 265 IEAGARLRYEPIALVAH----DHRTQLRDWIARKAFYGG 299 >NMB1929 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 349 Score = 55.8 bits (133), Expect = 8e-08, Method: Compositional matrix adjust. Identities = 64/265 (24%), Positives = 114/265 (43%), Gaps = 41/265 (15%) Query: 10 PKISIIICTYN--RSAXXXXXXXXXXXXENLDQAEVIVVDNSSKDDTAAVVKRFIEAEGA 67 P +S++IC YN + NLD +++VD+ S D T A+ +RF E +G Sbjct: 19 PLVSVLICAYNVEKYFAQSLAAVVNQTWRNLD---ILIVDDGSTDGTLAIAQRFQEQDGR 75 Query: 68 NMDIRYILEPVQ-GLSAARNTGI--LASK---SPLIAFLDDDAIPCRNWITTIVSTFEQK 121 IR + +P GL + N G+ LA IA D D I +WI IV E+ Sbjct: 76 ---IRILAQPRNSGLIPSLNIGLDELAKSGGGGEYIARTDADDIAAPDWIEKIVGEMEKD 132 Query: 122 PEVMAMGGKIAPIFESRRPDWLIKPFELPYTIVDLGNRVKEYPKR------LHPCG---- 171 ++AMG + + E + + L + E ++ + P R P G Sbjct: 133 RSIIAMGAWLEVLSEEKDGNRLARHHE--------HGKIWKKPTRHEDIADFFPFGNPIH 184 Query: 172 -ANMAMRKVAFDISLFPLELGRKGDSLLSGEETWLFNQIESQGHSILYHPQMAVDHFVPA 230 M MR+ D L + ++ E + F S+ + Y+P+ V + + A Sbjct: 185 NNTMIMRRSVIDGGL-------RYNTERDWAEDYQFWYDVSKLGRLAYYPEALVKYRLHA 237 Query: 231 NRLTEDWIMKRY-YSQGISNAMKSE 254 N+++ + ++++ +QGI +++ Sbjct: 238 NQVSSKYSIRQHEIAQGIQKTARND 262 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,544,823 Number of extensions: 834875 Number of successful extensions: 1423 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 1473 Number of HSP's successfully gapped: 13 Length of query: 313 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 207 Effective length of database: 26,153,027 Effective search space: 5413676589 Effective search space used: 5413676589 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)