BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0040.p (257 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value slr1118 [M] COG1922 Teichoic acid biosynthesis proteins 152 5e-37 all4422 [M] COG1922 Teichoic acid biosynthesis proteins 146 4e-35 STM0818 [M] COG0845 Membrane-fusion protein 138 8e-33 alr1705 [M] COG1922 Teichoic acid biosynthesis proteins 119 4e-27 AGc4311 [M] COG1922 Teichoic acid biosynthesis proteins 114 2e-25 >slr1118 [M] COG1922 Teichoic acid biosynthesis proteins Length = 251 Score = 152 bits (384), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 81/237 (34%), Positives = 122/237 (51%), Gaps = 4/237 (1%) Query: 5 NMFDVNFDNYDFMDLLDYIDKTIQERSQSYILTCNVDHVIKLRKDKEFQTVYSQAGAVVA 64 + D D+ I + SY++ NV V+ +K +Q + +QA V Sbjct: 6 TIISTRIDCTDYGQATATIINLAKAAIASYVVAANVHVVMSAYGNKSYQEIINQAVLVTP 65 Query: 65 DGMPLIWASKMLGKPLKQKVSGADLFSRLGNAFEQRKYRLFFLGSAQGVPEQATKNLKAA 124 DGMPL+W K+LG P +V G DL + ++ G + +Q NL Sbjct: 66 DGMPLVWGLKLLGYPQATRVYGPDLMLTICQRAAAENLGIYLYGGTEDCLQQLKVNLIKR 125 Query: 125 YPGMNIVGCYSPSY-GFEHNDEENQRIIEMLTESQPDIVFVGVGAPKQEKWIYRHYNSYR 183 +P + IVG SP + E +E+N + + +S I+FVG+G PKQE W+ HY Sbjct: 126 FPKLKIVGADSPPFRPLEPEEEKNA--LAKIKQSGAGILFVGLGCPKQEIWMANHYQKID 183 Query: 184 APISIGVGATFDFLSGSVKRAPNFMQKTGLEWFWRLSQEPGRLWKRYLVDDAQFLVL 240 + +GVGA F+F SG V +AP ++ GLEW +RL EP RLW+RYL+++ F+VL Sbjct: 184 L-VMVGVGAAFNFHSGLVSQAPRWLMAMGLEWLYRLRMEPKRLWRRYLINNPAFIVL 239 >all4422 [M] COG1922 Teichoic acid biosynthesis proteins Length = 257 Score = 146 bits (368), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 76/242 (31%), Positives = 130/242 (53%), Gaps = 3/242 (1%) Query: 6 MFDVNFDNYDFMDLLDYIDKTIQERSQSYILTCNVDHVIKLRKDKEFQTVYSQAGAVVAD 65 + V+ D +L I ++I++ + I N+ + KD E Q Y++A + D Sbjct: 9 LLGVSVDALSIPELNCLIGESIEQGKKWIIANHNLHSLYLFHKDLEMQAFYAKAEYIHID 68 Query: 66 GMPLIWASKMLGKPLK--QKVSGADLFSRLGNAFEQRKYRLFFLGSAQGVPEQATKNLKA 123 MP+++ K+LG P+K Q+V+ AD L + +R+F+LGS GV EQ L+ Sbjct: 69 SMPIVFIGKLLGFPMKREQRVTYADWVWPLMEEAANKSWRIFYLGSKPGVAEQGAVILRR 128 Query: 124 AYPGMNIVGCYSPSYGFEHNDEENQRIIEMLTESQPDIVFVGVGAPKQEKWIYRHYNSYR 183 +PG+ I C + EEN +I+ + + +P ++ VG+G P+QE WI ++ + Sbjct: 129 KFPGLKI-ACSHGYFDTSEESEENLTVIKAINDYKPHVLMVGMGMPRQEHWISQNLERIQ 187 Query: 184 APISIGVGATFDFLSGSVKRAPNFMQKTGLEWFWRLSQEPGRLWKRYLVDDAQFLVLLLK 243 + GA D+++G++ P +M + GLEW +RL EP RLW+RYL++ L L+ Sbjct: 188 PNAILTSGACLDYVAGAIPTPPRWMGRMGLEWLYRLLTEPTRLWRRYLLEPWFLGTLFLR 247 Query: 244 EL 245 E+ Sbjct: 248 EI 249 >STM0818 [M] COG0845 Membrane-fusion protein Length = 331 Score = 138 bits (348), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 6/251 (2%), Positives = 34/251 (13%), Gaps = 10/251 (3%) Query: 1 MNQVNMFDVNFDNYDFMDLLDYIDKTIQERSQSYILTCNVDHVIKLRKDKEFQTVYSQAG 60 + + + + + + + + + Sbjct: 32 GLTLYGNVDIRTVNISFRVGGRLASLNVDEGDTIKAGQVLGELDHAPYENALMQAKAGVS 91 Query: 61 AVVADGMPLIWASKMLGKPLKQKVSGADLFSRLGNAFEQRKYRLFFLGSAQGVPEQATKN 120 A ++ + + + + + + Sbjct: 92 VAQAQYDLMLAGYRDEEIAQAAAAVRQAQAAYDYAQNFYNRQQGLWKSRTISANDLENAR 151 Query: 121 LKAAYPGMNIVGCYSPSYGFEHNDEENQRIIEMLTESQPDIVFVGVGAPKQEKWIYRHYN 180 + + E + Q + + Sbjct: 152 SSRD--QAQATLKSA---QD-KLSQYRTGNREQDIAQAKASLEQAKAQLAQAQLDLQDTT 205 Query: 181 SYRAPISIGVGATFDFLSGSVKRAPNFMQKTGLEWFWRLSQEPGRLWKRYL-VDDAQFLV 239 + + S + + R + L Sbjct: 206 LIAPANGTLLTRAVEPGSMLNAGSTVLTLSLTRPVWVRAYVDE--RNLSQTQPGRDILLY 263 Query: 240 LLLKE-LRKRD 249 + Sbjct: 264 TDGRPDKPYHG 274 >alr1705 [M] COG1922 Teichoic acid biosynthesis proteins Length = 359 Score = 119 bits (298), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 13/216 (6%), Positives = 47/216 (21%), Gaps = 7/216 (3%) Query: 18 DLLDYIDKTIQERSQSYILTCNVDHVIKLRKDKEFQTVYSQAGAVVADGMPLIWASKMLG 77 + ++ + + D + V A+ + +L Sbjct: 20 LRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLE 79 Query: 78 KPLKQKVSGADLFSRLGNAF--EQRKYRLFFLGSAQGVPEQATKNLKAAYPGMNIVGCYS 135 + A E++ L + V + G+ + Sbjct: 80 GFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEA 139 Query: 136 PSYGFEHNDEENQRIIEMLTESQPDIVFVGVGAPKQEKWIYRHYNSYRAPISIGVGATFD 195 + H + + + + + EK + A Sbjct: 140 ---FY-HRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASG 195 Query: 196 FLSGSVKRAPNFMQKTGLEWFWRLSQEPGRLWKRYL 231 + + ++++ + + ++ + Sbjct: 196 GILLWPQSHFDWVRPGIILYGVSPLEDRST-GADFG 230 >AGc4311 [M] COG1922 Teichoic acid biosynthesis proteins Length = 211 Score = 114 bits (284), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 0/242 (0%), Positives = 0/242 (0%), Gaps = 22/242 (9%) Query: 20 LDYIDKTIQERSQSYILTCNVDHVIKLR----------------KDKEFQTVYSQAGAVV 63 + + + ++ + Q Sbjct: 50 ATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRA 109 Query: 64 ADGMPL-IWASKMLGKPLKQKVSGADLFSRLGNAFEQRKYRLFFLGSAQGVPEQATKNLK 122 A + R Q + Sbjct: 110 ALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPA 169 Query: 123 AAYPGMNIVGCYSPSYGFEHNDEENQRIIEMLTESQPDIVFVGVGAPKQEKWIYRHYNSY 182 + G Sbjct: 170 EFKVDCHHWLILH---GRYTCIARKP- 225 Query: 183 RAPISI-GVGATFDFLSGSVKRAPNFMQKTGLEWFWRLSQEPGRLWKRYLVDDAQFLVLL 241 Sbjct: 226 285 Query: 242 LK 243 Sbjct: 286 287 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.138 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,907,148 Number of extensions: 867069 Number of successful extensions: 11619 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 11609 Number of HSP's successfully gapped: 6 Length of query: 257 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 153 Effective length of database: 26,383,695 Effective search space: 4036705335 Effective search space used: 4036705335 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)