BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0035.p (380 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TM0622 [M] COG0438 Glycosyltransferase 77 6e-14 STM0622 [G] COG2301 Citrate lyase beta subunit 77 6e-14 CC1052 [M] COG0438 Glycosyltransferase 77 6e-14 MJ1178 [M] COG0438 Glycosyltransferase 69 9e-12 mll2084 [M] COG0438 Glycosyltransferase 68 3e-11 >TM0622 [M] COG0438 Glycosyltransferase Length = 388 Score = 76.6 bits (187), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 57/184 (30%), Positives = 93/184 (50%), Gaps = 7/184 (3%) Query: 196 KDFNVLL-VGRLAQWKGQHIVLEA-AKAFKNDSRVKFWLAGDALFGEEAYKNELIQKIQQ 253 +D +L+ V RL++ K +++ A +KA ++ ++ WL GD + EL++++ Sbjct: 194 RDKTILINVARLSREKNHALLVRAFSKAVQSCPNLELWLVGDGELRRDI--EELVKQLGL 251 Query: 254 DELTNVSLLGHVDDIQGLMSKADLLIHTSITPEPFGQVIVEGMAAGLPVIASNXXXXXXX 313 +E V G D+ L+S+AD+ + +S E FG V+ E MAAGLPVIA+ Sbjct: 252 EE--KVKFFGVRSDVPELLSQADIFVLSS-DYEGFGLVVAEAMAAGLPVIATAIGGIPEI 308 Query: 314 XXXXXXXLLIQPGDPVVLADSITWMLNHPEDRKRMADSGMKRVKEHFVIENTVKDIVDYY 373 +L+ P D LA +I + + R ++D G K V E F I TV++ Y Sbjct: 309 LEGGRAGILVPPKDVDALAKAIVELARDEKKRAELSDYGRKLVAERFDIRRTVREYEKLY 368 Query: 374 KGLL 377 LL Sbjct: 369 LELL 372 >STM0622 [G] COG2301 Citrate lyase beta subunit Length = 302 Score = 76.6 bits (187), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 0/184 (0%), Positives = 0/184 (0%), Gaps = 7/184 (3%) Query: 196 KDFNVLL-VGRLAQWKGQHIVLEA-AKAFKNDSRVKFWLAGDALFGEEAYKNELIQKIQQ 253 + + + + L+ A + K + + + + Sbjct: 194 RSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIELLHN--LYAPTRKEV 251 Query: 254 DELTNVSLLGHVDDIQGLMSKADLLIHTSITPEPFGQVIVEGMAAGLPVIASNXXXXXXX 313 ++ + L Sbjct: 252 AH--ARLVVEAAEAAAREGLGVVSLNGKM- 308 Query: 314 XXXXXXXLLIQPGDPVVLADSITWMLNHPEDRKRMADSGMKRVKEHFVIENTVKDIVDYY 373 Sbjct: 309 368 Query: 374 KGLL 377 Sbjct: 369 372 >CC1052 [M] COG0438 Glycosyltransferase Length = 349 Score = 76.6 bits (187), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 79/261 (30%), Positives = 115/261 (44%), Gaps = 35/261 (13%) Query: 92 ILKQEKVDCVHTNSLKSAFYGAVAAKKAGVPLIWHIRDHIGAPYLKPVVAKA--IRLLSR 149 ++++EKV VH S AF AA VP + A Y AK+ R + Sbjct: 42 LIRREKVSLVHARSRAPAFSALWAAHATKVPFV--------ATYHGVYNAKSGLKRWYNA 93 Query: 150 LLPNG--VIANSHSTL------------------NALELPRTKKTLVVYSAFAKAIGEGI 189 ++ G VIANS T ++L R + VV + +A+ + Sbjct: 94 VMTKGAVVIANSEYTREHVIREHGVAADKVVAIPRGVDLSRFEPD-VVSADRIEALRQAW 152 Query: 190 GKREQQKDFNVLLVGRLAQWKGQHIVLEAAKAFK--NDSRVKFWLAGDALFGEEAYKNEL 247 G ++ VLL GRL +WKGQ ++++A K + +V LAGD G + Y+ EL Sbjct: 153 GVAPDERRLKVLLAGRLTRWKGQGLLVQAMALLKARGEDKVLLLLAGDDQ-GRKGYRAEL 211 Query: 248 IQKIQQDELTN-VSLLGHVDDIQGLMSKADLLIHTSITPEPFGQVIVEGMAAGLPVIASN 306 I Q L + V L+GH DD+ ADL I S+ PE FG+ VE G PV+A++ Sbjct: 212 AAAIAQAGLEDAVKLVGHCDDMPAAYLLADLAIAPSLEPEAFGRTAVEPQVMGRPVMAAD 271 Query: 307 XXXXXXXXXXXXXXLLIQPGD 327 L+ PGD Sbjct: 272 HGATRETVLPGETGWLVTPGD 292 >MJ1178 [M] COG0438 Glycosyltransferase Length = 351 Score = 69.3 bits (168), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 73/293 (24%), Positives = 122/293 (41%), Gaps = 29/293 (9%) Query: 92 ILKQEKVDCVHTN-SLKSAFYGAVAAKKAGVPLIWHIRDHIGAPYLKPVVAKAIRLLSRL 150 I++ E +D +H++ + GA+ K +P I + + + + Sbjct: 78 IIESEGIDLIHSHYAFPQGCVGALLKNKLSIPHILTLHGSDALILKNSIKGRYFFKYATT 137 Query: 151 LPNGVIANSHSTLNALELPRTKKTLVVYSAFAKAIGEGIGKREQQKDFNV-LLVGRLAQW 209 + +I S N L+ + +V+Y+ K I + D+N L VG Sbjct: 138 NSDKIICVSKYIKNQLDENLKNRAIVIYNGVNKEI------LYNEGDYNFGLFVGAFVPQ 191 Query: 210 KGQHIVLEAAKAFKNDSRVKFWLAGDALFGEEAYKNELIQKIQ----QDELTNVSLLGH- 264 KG I+++A K D F L GD +L +KI+ ++ L+++ LLG Sbjct: 192 KGVDILIDAIK----DIDFNFKLIGDG---------KLYKKIENFVVKNNLSHIELLGRK 238 Query: 265 -VDDIQGLMSKADLLIHTSITPEPFGQVIVEGMAAGLPVIASNXXXXXXXXXXXXXXLLI 323 D++ M K L+ S E FG V VEGMA PVIA+ LL Sbjct: 239 SFDEVASFMRKCSFLVVPS-RSEGFGMVAVEGMACSKPVIATRVGGLGEIVIDGYNGLLA 297 Query: 324 QPGDPVVLADSITWMLNHPEDRKRMADSGMKRVKEHFVIENTVKDIVDYYKGL 376 + +P L + I ++N+ E RK + ++G K + F E V + Y+ L Sbjct: 298 EKNNPNDLKEKILELINNEELRKTLGENG-KEFSKKFSWEKCVMGVRKVYEEL 349 >mll2084 [M] COG0438 Glycosyltransferase Length = 301 Score = 67.8 bits (164), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 0/209 (0%), Positives = 0/209 (0%), Gaps = 4/209 (1%) Query: 155 VIANSHSTLNALELPRTKKTLVVYSAFAKAIGEGIGKREQQKDFNVLLVGRLAQWKGQHI 214 + + + + + + F G + Sbjct: 178 HHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILV 237 Query: 215 VLEAAKAFKND--SRVKFWLAGDALFGEEAYKNELIQKIQQDEL-TNVSLLGHVDDIQGL 271 E K + +A + E ++ + Sbjct: 238 EREGQKKMVIGNKGAKIKTIGIEA-RKDMQEMFEAPVHLELWVKVKSGWADDERALRSLG 296 Query: 272 MSKADLLIHTSITPEPFGQVIVEGMAAGLPVIASNXXXXXXXXXXXXXXLLIQPGDPVVL 331 Sbjct: 297 356 Query: 332 ADSITWMLNHPEDRKRMADSGMKRVKEHF 360 Sbjct: 357 385 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,284,576 Number of extensions: 883913 Number of successful extensions: 1757 Number of sequences better than 1.0e-05: 39 Number of HSP's gapped: 1771 Number of HSP's successfully gapped: 39 Length of query: 380 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 272 Effective length of database: 25,922,359 Effective search space: 7050881648 Effective search space used: 7050881648 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)