BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0034.p (378 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DRA0040 [M] COG0438 Glycosyltransferase 221 1e-57 SMb21075 [M] COG0438 Glycosyltransferase 209 7e-54 MA1175 [M] COG0438 Glycosyltransferase 158 1e-38 AF0043 [M] COG0438 Glycosyltransferase 150 3e-36 AF0606 [M] COG0438 Glycosyltransferase 146 6e-35 >DRA0040 [M] COG0438 Glycosyltransferase Length = 301 Score = 221 bits (564), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 0/361 (0%), Positives = 0/361 (0%), Gaps = 5/361 (1%) Query: 13 MKIAIAHDYLIQMGGAERVVEVFHHMYPDAPIFTTVFNGSRLTDNLKDADIRASWLQKIP 72 M I ++ I + G V + + ++ T + Q I Sbjct: 1 MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIY 60 Query: 73 GVKTNFKGVLPLYPMAIRDLDFRGYDIVLSSSSAFMKSIQVPESTFHLCYCHTPMRFAWD 132 + R ++ + + + Sbjct: 61 --VDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAP 118 Query: 133 -YDTYMERQSNSGLFKRLL-KVYMQRLKHWDQRTSKNVNQFVANSSVVKTRIQNYYHRDA 190 + + L ++ ++ + + ++ ++ Sbjct: 119 VILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATH 178 Query: 191 DVIFPPINTARFSSSSSIGDYYLIVSRLVSYKRIDLAVEAFNRNGLKLFIVGDGPDRKRL 250 I +S ++ L + + VE + Sbjct: 179 HFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVE 238 Query: 251 EGMAKDNVSFLGRLEDAEVTGLMSKCRAFIFPGEEDFGITPLEANAAGRPVIAYQAGGAL 310 K V + + K +F + + A ++ G + Sbjct: 239 REGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALRSLGYV 298 Query: 311 DTIVPYVNGVFFQRQEVDDLLKAIYEVESYAWDIGQ-IMNHARKFDEQAFMVQFKQYVEQ 369 Sbjct: 299 358 Query: 370 A 370 Sbjct: 359 359 >SMb21075 [M] COG0438 Glycosyltransferase Length = 410 Score = 209 bits (531), Expect = 7e-54, Method: Compositional matrix adjust. Identities = 125/361 (34%), Positives = 187/361 (51%), Gaps = 18/361 (4%) Query: 14 KIAIAHDYLIQMGGAERVVEVFHHMYPDAPIFTTVFNGSRLTDNLK-----DADIRASWL 68 ++AI HD+ G ERV+ ++P+A +FT +F+ L ++K D +S Sbjct: 35 RLAIVHDWCPNFRGGERVLARICKLFPNAEVFT-LFDF--LPKDIKEQYFHDVAFHSSVA 91 Query: 69 QKIPGVKTNFKGVLPLYPMAIRDLDFRGYDIVLSSSSAFMKSIQVPESTFHLCYCHTPMR 128 ++P V+ ++ + L P I D GYD V+SSS+AF + + HLCY H+P+R Sbjct: 92 NRLPLVQKYYRSLFFLCPFLIEQFDVTGYDAVISSSAAFARGVLTRPDQPHLCYVHSPIR 151 Query: 129 FAWDYDTYMERQSNSGLFKR--LLKVYMQRLKHWDQRTSKNVNQFVANSSVVKTRIQNYY 186 +AWD Q G + L + + L+ WD RT+ + +ANSS V++RIQ Y Sbjct: 152 YAWDEQFSYLAQGRLGFGPKGLLFRYMLHNLRTWDTRTAHGPDLMLANSSYVRSRIQRIY 211 Query: 187 HRDADVIFPPINTARFSSSSSIGDYYLIVSRLVSYKRIDLAVEAFNR-NGLKLFIVGDGP 245 R+A+V+FPP+ + DYY+ S L YKR DL + AFN +L +VG+G Sbjct: 212 GREAEVVFPPVALDELEFIACKDDYYVTASFLAPYKRTDLVIRAFNEMPSRRLVVVGEGQ 271 Query: 246 DRKRLEGMAKDNVSFLGRLEDAEVTGLMSKCRAFIFPGEEDFGITPLEANAAGRPVIAYQ 305 + L GMA NVSF G L E +++ +A +F G EDFGI EA A G P+I + Sbjct: 272 QSQSLRGMASSNVSFTGYLPRQEYVRTVARAQAMVFAGCEDFGIALAEAQACGTPLIVFG 331 Query: 306 AGGALDTIVPY-----VNGVFFQRQEVDDLLKA--IYEVESYAWDIGQIMNHARKFDEQA 358 GGA D + P+ G+ F Q V+ L +A I+E A ++A +F E Sbjct: 332 RGGARDIVQPFGEAEHPTGILFGIQSVESLKEAIHIFEQNQPAIKSQACRSNADRFSEGR 391 Query: 359 F 359 F Sbjct: 392 F 392 >MA1175 [M] COG0438 Glycosyltransferase Length = 356 Score = 158 bits (400), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 118/370 (31%), Positives = 188/370 (50%), Gaps = 33/370 (8%) Query: 13 MKIAIAHDYLIQMGGAERVVEVFHHMYPDAPIFTTVFNGSRLTDNLKDADIRASWLQKIP 72 MK AI HDY +GG E+VV ++ +A I TT + D + I QKIP Sbjct: 1 MKTAIFHDYFGAIGGGEKVVITLANLL-NADIITTDMDAVDKLDTEVNV-ISLGHTQKIP 58 Query: 73 GVKTNFKGVLPLYPMAI-RDLDFRGYDIVLSSSSAFMKSIQVPESTFHLCYCHTPMRFAW 131 +K Y D DF I + S + P ++ YC+TP+R + Sbjct: 59 PLK-QISATEKFYSCNFSNDYDFF---IFTGNWSHYAAHRHHP----NMWYCYTPVRAFY 110 Query: 132 D-YDTYMERQSNSGLFKRLLKVYMQRLKHWDQRTSKNVNQFVANSSVVKTRIQNYYHRDA 190 D YDT+++RQ + ++ ++++ + DQ++ +N++Q V S + RI+ Y+HR+A Sbjct: 111 DLYDTFLQRQD--FITRQAFRIWVYGHRWLDQKSIRNIDQIVTISKNSQKRIEKYHHRNA 168 Query: 191 DVIFPPINTARFSSSSSIGDYYLIVSRLVSYKRIDLAVEAFNR-NGLKLFIVGDGPDRKR 249 D+I+PP++ ++F GD++L V+RL KR++L +EAF + KL IVG Sbjct: 169 DIIYPPVDVSKF-ECKEYGDFWLSVNRLYPEKRVELQIEAFRKMPDEKLIIVGGYAKGDH 227 Query: 250 LEGMAK-------DNVSFLGRLEDAEVTGLMSKCRAFIFPG-EEDFGITPLEANAAGRPV 301 E AK DNV+ LG + + E+ L S+CR FI +EDFG+TP+EA A+G+PV Sbjct: 228 AEKYAKHISNKLPDNVNILGEVSETELIDLYSRCRGFICTALDEDFGLTPVEAMASGKPV 287 Query: 302 IAYQAGGALDTIVPYVNGVFFQRQEVDDLLKAIY----EVESYAWDIGQIMNHARKFDEQ 357 +A GG +T+ F ++ +++A+ E ESY + A+ FD Sbjct: 288 VAANEGGFKETVTEKTG--FLINADIHSVIEAVKFVSREPESYR---DACLKQAKCFDLS 342 Query: 358 AFMVQFKQYV 367 F + K V Sbjct: 343 IFSEKIKNVV 352 >AF0043 [M] COG0438 Glycosyltransferase Length = 363 Score = 150 bits (379), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 109/373 (29%), Positives = 194/373 (52%), Gaps = 26/373 (6%) Query: 13 MKIAIAHDYLIQMGGAERVVEVFHHMYPDAPIFTTVFNGSRLTDNLKDADIRASWLQKIP 72 MKI + HDY +GG E+V ++ +A + TT + + + ++ I K+P Sbjct: 1 MKICVFHDYFGAIGGGEKVALTISKLF-NADVITTDVDA--VPEEFRNKVISLGETIKLP 57 Query: 73 GVKTNFKGVLPLYPMAIRDLDFRGYDIVLSSSSAFMKSIQVPESTFHLCYCHTPMRFAWD 132 +K L Y D DF I+ + F +P +L YC+TP R +D Sbjct: 58 PLK-QIDASLKFYFSDFPDYDFY---ILSGNWVMFASKRHIP----NLLYCYTPPRAFYD 109 Query: 133 -YDTYMERQSNSGLFKRLLKVYMQRLKHWDQRTSKNVNQFVANSSVVKTRIQNYYHRDAD 191 Y Y+++++ L K ++++ + W +R K++++ V S +K+R +N++ DA+ Sbjct: 110 LYGDYLKKRN--ILTKPAFILWVKFHRKWAERMLKHIDKVVCISQNIKSRCKNFWGIDAE 167 Query: 192 VIFPPINTARFSSSSSIGDYYLIVSRLVSYKRIDLAVEAFNR-NGLKLFIV-----GDGP 245 VI+PP+ T++F GD++L V+R+ KRI+L +E F + KL+IV GD Sbjct: 168 VIYPPVETSKF-KFKCYGDFWLSVNRIYPEKRIELQLEVFKKLQDEKLYIVGWFSKGDHA 226 Query: 246 DR--KRLEGMAKDNVSFLGRLEDAEVTGLMSKCRAFIFPG-EEDFGITPLEANAAGRPVI 302 +R +++ +A DNV FLG + + E+ L S+C+ + +EDFG+TP+EA A+G+PVI Sbjct: 227 ERYARKIMKIAPDNVKFLGSVSEEELIDLYSRCKGLLCTAKDEDFGLTPIEAMASGKPVI 286 Query: 303 AYQAGGALDTIVPYVNGVFFQRQEVDDLLKAIYEVESYAWDIGQ-IMNHARKFDEQAFMV 361 A GG +T++ G + +V++++ A+ +V + A++FD F Sbjct: 287 AVNEGGFKETVINEKTG-YLVNADVNEIIDAMKKVSKNPDKFKKDCFRRAKEFDISIFKN 345 Query: 362 QFKQYVEQAYVNF 374 + K + NF Sbjct: 346 KIKDAIRIVKKNF 358 >AF0606 [M] COG0438 Glycosyltransferase Length = 363 Score = 146 bits (368), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 108/371 (29%), Positives = 181/371 (48%), Gaps = 22/371 (5%) Query: 13 MKIAIAHDYLIQMGGAERVVEVFHHMYPDAPIFTTVFNGSRLTDNLKDADIRASWLQKIP 72 MKIA+ H+ ++GG E ++ + ++ +++ V + + T +D + L + Sbjct: 1 MKIAVFHESFGEIGGGE-ILASYLGRALNSKVYSIVKDENINTLGFEDI---SQHLPLVA 56 Query: 73 GVKTNFKGVLPLYPMAIRDLDFRGYDIVLSSSSAFMKSIQVPESTFHLCYCHTPMRFAWD 132 + F+ L + DF YD+V++S + + P+ H+ Y H+P R W Sbjct: 57 KILRRFRSFDYLTWSNVDVRDFGDYDVVITSGLT-ARVLITPDDVMHVHYNHSPAR--WL 113 Query: 133 YDTYMERQSNSGLFKR--LLKVYMQRLKHWDQRTSKNVNQFVANSSVVKTRIQNYYHRDA 190 YD + R+ + G KR LL + + + WD K V+ + +NS V K R+ Y RD+ Sbjct: 114 YDLWHHRRKSKGWLKRNVLLPLASEFFRIWDTAVDKRVDYYFSNSPVTKRRLWKYLKRDS 173 Query: 191 DVIFPPINTARFSSSSSIGDYYLIVSRLVSYKRIDLAVEAFNRNGLKLFIVGDGPDRKRL 250 V++PPI ++ +S D+YL V RL KR + A+ + K+ ++G G K L Sbjct: 174 VVLYPPIEFDKYKCKNS-EDFYLFVGRLWHEKRPEEAIRGCIKAKKKIVVIGSGYLEKYL 232 Query: 251 EGMAKDN--VSFLGRLEDAEVTGLMSKCRAFIFPG-EEDFGITPLEANAAGRPVIAYQAG 307 N V G + + E L++ C+A I+P EDFGI P+EA A+G+PVIA +G Sbjct: 233 RDKYGKNPYVEIKGFVSEEEKVDLLASCKAVIYPCIAEDFGIVPIEAFASGKPVIADNSG 292 Query: 308 GALDTIVPYV----NGVFFQRQEVDDLLKAIYEVESYAWDIGQIMNHARKFDEQAFMVQF 363 PYV G+ D++ KAI E+E +D +I A++FD F Sbjct: 293 -----FPPYVVNEERGIITDCSNPDNIAKAIEELEKKKYDPDKIREFAKQFDFSVFKENL 347 Query: 364 KQYVEQAYVNF 374 ++++ Y F Sbjct: 348 WYWLKRWYDEF 358 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.139 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,279,670 Number of extensions: 1083719 Number of successful extensions: 3318 Number of sequences better than 1.0e-05: 72 Number of HSP's gapped: 3303 Number of HSP's successfully gapped: 72 Length of query: 378 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 270 Effective length of database: 25,922,359 Effective search space: 6999036930 Effective search space used: 6999036930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)