BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0031.p (457 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC3058 [M] COG0836 Mannose-1-phosphate guanylyltransferase 150 5e-36 CAC3072 [M] COG0836 Mannose-1-phosphate guanylyltransferase 141 2e-33 PA3551_1 [M] COG0836 Mannose-1-phosphate guanylyltransferase 135 1e-31 STM1033_1 [OU] COG0740 Protease subunit of ATP-dependent Clp pro... 132 2e-30 BMEII0900_1 [M] COG0836 Mannose-1-phosphate guanylyltransferase 118 2e-26 >CAC3058 [M] COG0836 Mannose-1-phosphate guanylyltransferase Length = 350 Score = 150 bits (378), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 102/345 (29%), Positives = 179/345 (51%), Gaps = 34/345 (9%) Query: 4 VLLSGGSGKRLWPLSNDSRSKQFLKVLQSPAGEPESMVQRVWRQLQETGMADSSYLATGR 63 ++++GG G+R WPLS D + KQFL++L ++M+Q +L++ + ++ TG Sbjct: 5 LIMAGGKGERFWPLSTDEKPKQFLQLLSD-----KTMIQMTVERLEDLIDIERIFVVTGE 59 Query: 64 SQVEMIQSQLG--AEVPIIVEPERRDTFPAIALTAAYLYSIAGVSPRETVAILPVDPYV- 120 V++++ QL E IIVEP ++T P IAL+A + I + +LP D + Sbjct: 60 RYVDLLKEQLPNLPEKNIIVEPVGKNTAPCIALSA---FVINKHYKDANIVVLPSDQLIV 116 Query: 121 -EASFFETVVQLEATIQESGANLALMGVVPELASEKYGYIIPTSDEAAASGYM--QVSHF 177 ++ +T+ E I ++ A++ +G+ P A YGYI + E SG+ +V F Sbjct: 117 DDSKLLDTIAAAEKFIDQNKASIVTLGMEPTRAETGYGYIKSGNLEKEISGFQIKKVESF 176 Query: 178 QEKPDRAQAEELISRGA-LWNCGVFAFRLGYLLDILQR-------------KGLPLNYEE 223 EKPD+ AE + G LWN G+F ++ +L + ++ + N+ E Sbjct: 177 VEKPDKKSAESYVEAGNFLWNGGMFIWKCSTILKLTEKYLNKTYNILNEIAEANDTNFNE 236 Query: 224 -LQKQYKLLSSISFDYEVVEKEENIVVQPYAGFWKDLGTWNTL---TEEMSSNHVGKGFV 279 L+K+Y + +IS DY ++EK +NI V P + W D+GTW+ + ++ N+V G + Sbjct: 237 TLKKKYVDVEAISVDYGIMEKADNIYVIPCSFGWDDIGTWHAVERYRDKDEDNNVCVGDI 296 Query: 280 TA-DSEGTCLINELDIPVTVIGAKDLIIAASPDGILVTHKTESPR 323 + DS+ L+ + P+ V+G ++ + S D I V K + R Sbjct: 297 KSIDSKNNILVGKKK-PIVVVGLSEVFVVESDDIIFVGKKEDIER 340 >CAC3072 [M] COG0836 Mannose-1-phosphate guanylyltransferase Length = 350 Score = 141 bits (355), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 101/346 (29%), Positives = 176/346 (50%), Gaps = 36/346 (10%) Query: 4 VLLSGGSGKRLWPLSNDSRSKQFLKVLQSPAGEPESMVQRVWRQLQETGMADSSYLATGR 63 ++++GG G+R WPLS D + KQFLK+L ++M+Q +L+ + ++ TG Sbjct: 5 LIMAGGKGERFWPLSTDEKPKQFLKLLSD-----KTMIQMTVERLESLIDIEKIFVVTGE 59 Query: 64 SQVEMIQSQLGAEVP---IIVEPERRDTFPAIALTAAYLYSIAGVSPRETVAILPVDPYV 120 V++++ QL +P IIVEP R+T P IAL+A + I ++ +LP D + Sbjct: 60 RYVDILREQL-PRLPKRNIIVEPVGRNTAPCIALSA---FVINKYYKDASIVVLPSDHLI 115 Query: 121 --EASFFETVVQLEATIQESGANLALMGVVPELASEKYGYIIPTSDEAAASGY--MQVSH 176 E + + E I+++ ++ +G+ P A YGYI + SG+ +V Sbjct: 116 VNEDKLLDAIRSAEEFIEKNDNSIVTLGMEPTRAETGYGYIKSGEVKEEISGFKIKKVDE 175 Query: 177 FQEKPDRAQAEELISRGA-LWNCGVFAFRLGYLLDILQ----------RKGLPLNYEE-- 223 F EKP++ +AEE + G LWN G+F ++ + + + R N +E Sbjct: 176 FVEKPNKEKAEEYVKAGNFLWNGGMFIWKCSTIFKLTEKYLNKTYNILRSISEANGDEFN 235 Query: 224 --LQKQYKLLSSISFDYEVVEKEENIVVQPYAGFWKDLGTWNTL---TEEMSSNHVGKGF 278 L+++Y + +IS DY ++EK ++I V P W D+GTW+ + ++ N+V G Sbjct: 236 VLLKREYVKVDNISVDYGIMEKADSIYVIPCDFGWDDIGTWHAVERYRDKDEDNNVCVGD 295 Query: 279 VTA-DSEGTCLINELDIPVTVIGAKDLIIAASPDGILVTHKTESPR 323 + + DS+ L+ + P+ V+G D+ + S D I V K + R Sbjct: 296 IKSIDSKNNILVGKKK-PIVVVGLSDVFVVESDDIIFVGKKDDIER 340 >PA3551_1 [M] COG0836 Mannose-1-phosphate guanylyltransferase Length = 332 Score = 135 bits (341), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 100/339 (29%), Positives = 160/339 (47%), Gaps = 35/339 (10%) Query: 4 VLLSGGSGKRLWPLSNDSRSKQFLKVLQSPAGEPESMVQRVWRQLQETGMADSSYLATGR 63 V+LSGGSG RLWPLS KQFL + +++ Q+ ++L GM + L + Sbjct: 4 VILSGGSGSRLWPLSRKQYPKQFLALTGD-----DTLFQQTIKRLAFDGM-QAPLLVCNK 57 Query: 64 SQVEMIQSQLGAE----VPIIVEPERRDTFPAIALTAAYLYSIAGVSPRETVAILPVDPY 119 ++Q QL A+ I++EP R+T PA+A+ A L + E + ILP D Sbjct: 58 EHRFIVQEQLEAQNLASQAILLEPFGRNTAPAVAIAAMKLVA---EGRDELLLILPADHV 114 Query: 120 VE-ASFFETVVQLEATIQESGANLALMGVVPELASEKYGYIIPTSDEAAASGYMQVSHFQ 178 +E F+ + L E G + L G+ YGYI ++D G +V F Sbjct: 115 IEDQRAFQQALALATNAAEKG-EMVLFGIPASRPETGYGYIRASADAQLPEGVSRVQSFV 173 Query: 179 EKPDRAQAEELISRGA-LWNCGVFAFRLGYLLDILQRKGLP------LNYEELQKQ---- 227 EKPD A+A E ++ G WN G+F FR L+ L++ L E Q Sbjct: 174 EKPDEARAREFVAAGGYYWNSGMFLFRASRYLEELKKHDADIYDTCLLALERSQHDGDLV 233 Query: 228 ------YKLLSSISFDYEVVEKEENIVVQPYAGFWKDLGTWNTLTE---EMSSNHVGKGF 278 ++ S DY V+EK V P + W D+G+W+++ + + ++ +V KG Sbjct: 234 NIDAATFECCPDNSIDYAVMEKTSRACVVPLSAGWNDVGSWSSIWDVHAKDANGNVTKGD 293 Query: 279 VTADSEGTCLINELDIPVTVIGAKDLIIAASPDGILVTH 317 V CL++ V+VIG +D+++ + D +++ H Sbjct: 294 VLVHDSHNCLVHGNGKLVSVIGLEDIVVVETKDAMMIAH 332 >STM1033_1 [OU] COG0740 Protease subunit of ATP-dependent Clp proteases Length = 200 Score = 132 bits (331), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 0/348 (0%), Positives = 0/348 (0%), Gaps = 34/348 (9%) Query: 1 MKLVLLSGGSGKRLWPLSNDSRSKQFLKVLQSPAGEPESMVQRVWRQLQETGMADSSYLA 60 V S +++ + R E G S Sbjct: 2 KTTVAAIKMPPVMTAGNGRGEGSSNSWYSIRA-----AANNTAEVRIYDEIGGWGISARW 56 Query: 61 TGRSQVEMIQSQLGAEVP---IIVEPERRDTFPAIALTAAYLYSIAGVSPRETVAILPVD 117 + Q + + + Sbjct: 57 FAEELAALGQINR-INLHIHSPGGAVLDGIAIYNLLKNHPA------QKTVYIDGMACSM 109 Query: 118 PYVEAS--FFETVVQ-LEATIQESGANLALMGVVPELASEKYGYIIPTSDEAAASGYMQV 174 A + + I + G ++ + Y + Sbjct: 110 ASAIAMVGNPIIMPENAMMMIHKPR--GVAGGEAEDIREYADLLD--KIESVIIPIYTEK 165 Query: 175 SHFQEKPDRAQAEELISRGA-LWNCGVFAFRLGYLLDILQ----------RKGLPLNYEE 223 + + A + Sbjct: 166 TGKTPEDIAAMLAKETWMSGA 225 Query: 224 LQKQYKLLSSISFDYEVVEKEENIVVQPYAGFWKDLGTWNTLTEEMSSNHVGKGFVTADS 283 Sbjct: 226 285 Query: 284 EGTCLINELDIPVTVIGAKDLIIAASPDGILVTHKTESPRIKEVLKSF 331 Sbjct: 286 - 332 >BMEII0900_1 [M] COG0836 Mannose-1-phosphate guanylyltransferase Length = 334 Score = 118 bits (296), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 98/347 (28%), Positives = 164/347 (47%), Gaps = 51/347 (14%) Query: 4 VLLSGGSGKRLWPLSNDSRSKQFLKVLQSPAGEPESMVQRVWRQLQETGMADSSYLATGR 63 V++SGGSG RLWPLS D+ K F+K+ P G E+++ + + + A+ T R Sbjct: 6 VIISGGSGSRLWPLSRDAHPKPFIKL---PDG--ETLIGKTYARASRLVNAEQILTVTNR 60 Query: 64 SQVEMIQSQLGA------EVPIIVEPERRDTFPAIALTAAYLYSIAGVSPRETVAILPVD 117 + + A E ++EP RDT PA+AL A + G P T+ ++P D Sbjct: 61 DFLFLTLDAYAAAGAAQMENTFLLEPLGRDTAPAVALAALHAAEAYG--PDATLLVMPAD 118 Query: 118 PYV--EASFFETVVQLEATIQESGANLALMGVVPELASEKYGYIIPTSDEAAASGYMQVS 175 + E +F E V + A + E+G + G+VP+ +GYI E + V Sbjct: 119 HLIEDEQAFAEAVAKARA-LAEAG-RIVTFGIVPDRPETGFGYI-----EVQGT---DVQ 168 Query: 176 HFQEKPDRAQAEELISRGA-LWNCGVFAFRLGYLLDILQR-------------------- 214 F EKPD A A+ + G WN G+F F+ ++D +QR Sbjct: 169 RFVEKPDEATAQTYVESGRYFWNSGMFCFKALSMIDAMQRYAPQVLAGARAALAQARRGN 228 Query: 215 KGLPLNYEELQKQYKLLSSISFDYEVVEKEENIVVQPYAGFWKDLGTWNTLTEEMSSNHV 274 G E + ++ +IS DY V+EK +N+ P + W D+G+W + + ++ + Sbjct: 229 NGETKTLEIARDEFAATPAISIDYAVMEKADNMACVPVSCGWSDIGSWAAMADLVTPDEN 288 Query: 275 GKGF----VTADSEGTCLINELDIPVTVIGAKDLIIAASPDGILVTH 317 G V D+ + +++E + V+++G DL++ +PD +LV H Sbjct: 289 GNRLRGETVLEDTTNSFVLSETRL-VSLVGVHDLLVVDTPDALLVAH 334 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,283,000 Number of extensions: 1432200 Number of successful extensions: 4537 Number of sequences better than 1.0e-05: 37 Number of HSP's gapped: 4449 Number of HSP's successfully gapped: 38 Length of query: 457 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 347 Effective length of database: 25,691,691 Effective search space: 8915016777 Effective search space used: 8915016777 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)