BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0001.p (183 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc02439 [H] COG0262 Dihydrofolate reductase 109 3e-24 mlr2644 [H] COG0262 Dihydrofolate reductase 103 1e-22 BS_yyaP [H] COG0262 Dihydrofolate reductase 91 1e-18 mlr0926 [H] COG0262 Dihydrofolate reductase 86 2e-17 mll8258 [H] COG0262 Dihydrofolate reductase 84 1e-16 >SMc02439 [H] COG0262 Dihydrofolate reductase Length = 86 Score = 109 bits (272), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 0/187 (0%), Positives = 0/187 (0%), Gaps = 15/187 (8%) Query: 1 MGQVILMMNVTLDGCC-----DHTQVIADEELHQYTVDLMDQSAGLLFGRKIYQLMESAW 55 M + D ++ EL S + Y ++E+A Sbjct: 1 MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASDLVSRKNVEYLVVEAAG 60 Query: 56 PAIASSGDGPQFMVDFARKLDRKPKYVFSRTLDHVSWQNSFLLKGPVSEEVPRLTA-EGK 114 + Sbjct: 61 E---TREL------ 111 Query: 115 NLVVNGGPGLGSTLAQLGLVDEYHFLVQPIVAGRGPRLLGGVLDRLNLKLAETRPFGSGV 174 Sbjct: 112 171 Query: 175 VVLRYTP 181 Sbjct: 172 178 >mlr2644 [H] COG0262 Dihydrofolate reductase Length = 86 Score = 103 bits (258), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 0/189 (0%), Positives = 0/189 (0%), Gaps = 17/189 (8%) Query: 1 MGQVILMMNVTLDGCCD---------HTQVIADEELHQYTVDLMDQSAGLLFGRKIYQLM 51 M + D + E + + + Sbjct: 1 MSMNDTYQPINCDDYDNLELACQHHLMLT---LELKDGEKLQAKASDLVSRKNVEYLVVE 57 Query: 52 ESAWPAIASSGDGPQFMVDFARKLDRKPKYVFSRTLDHVSWQNSFLLK-GPVSEEVPRLT 110 + F Sbjct: 58 AAGETRELRLDKITSF---- 113 Query: 111 AEGKNLVVNGGPGLGSTLAQLGLVDEYHFLVQPIVAGRGPRLLGGVLDRLNLKLAETRPF 170 Sbjct: 114 173 Query: 171 GSGVVVLRY 179 Sbjct: 174 182 >BS_yyaP [H] COG0262 Dihydrofolate reductase Length = 86 Score = 90.9 bits (224), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 0/183 (0%), Positives = 0/183 (0%), Gaps = 13/183 (7%) Query: 3 QVILMMNVTLDGCCDHTQ-----VIADEELHQYTVDLMDQSAGLLFGRKIYQLMESAWPA 57 + L+ C H + E+L S + + Sbjct: 9 PINCDDYDNLELACQHHLMLTLELKDGEKLQ--AKASDLVSRKNVEYLVVEAA----GET 62 Query: 58 IASSGDGPQFMVDFARKLDRKPKYVFSRTLDHVSWQNSFLLKGPVSEEVPRLTAE-GKNL 116 Sbjct: 63 - 121 Query: 117 VVNGGPGLGSTLAQLGLVDEYHFLVQPIVAGRGPRLLGGVLDRLNLKLAETRPFGSGVVV 176 Sbjct: 122 181 Query: 177 LRY 179 Sbjct: 182 184 >mlr0926 [H] COG0262 Dihydrofolate reductase Length = 86 Score = 86.3 bits (212), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 0/188 (0%), Positives = 0/188 (0%), Gaps = 10/188 (5%) Query: 1 MGQVILMMNVTLDGCC-----DHTQVIADEELHQYTVDLMDQSAGLLFGRKIYQLMESAW 55 M + D ++ EL S + Y ++E+ Sbjct: 1 MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASDLVSRKNVEYLVVEA-A 59 Query: 56 PAIASSGDGPQFMVDFARKLDRKPKYVFSRTLDHVSWQNSFLLKGPVSEEVPRLTAEGKN 115 Sbjct: 60 - 118 Query: 116 LVVNGGPGLGSTLAQLGLVDEYHFLVQPIVAGRG-PRLLGGVLDRLNLKLAETRPFGSGV 174 Sbjct: 119 -- 176 Query: 175 VVLRYTPV 182 Sbjct: 177 184 >mll8258 [H] COG0262 Dihydrofolate reductase Length = 86 Score = 84.3 bits (207), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 0/186 (0%), Positives = 0/186 (0%), Gaps = 14/186 (7%) Query: 1 MGQVILMMNVTLDGCCDHTQ---VIADEELHQYTVDLMDQSAGLLFGRKIYQLMESAWPA 57 M + D + L + + A L RK + + Sbjct: 1 MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASDLVSRKNVEYLVVEAA- 59 Query: 58 IASSGDGPQFMVDFARKLDRKPKYVFSRTLDHVSWQNSFLLKGPVSEEVPRLT-AEGKNL 116 Sbjct: 60 -------- 111 Query: 117 VVNGGPGLGSTLAQLGLVDEYHFLVQPIVAGRGPRLLGGVLDRLNLKLAETRPFGSGVVV 176 Sbjct: 112 - 170 Query: 177 LRYTPV 182 Sbjct: 171 176 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.140 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,080,141 Number of extensions: 599156 Number of successful extensions: 1159 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 1145 Number of HSP's successfully gapped: 8 Length of query: 183 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 83 Effective length of database: 26,845,031 Effective search space: 2228137573 Effective search space used: 2228137573 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 112 (47.8 bits)