BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0151.0012.p (342 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value alr1407 [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 219 3e-57 alr2968 [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 219 6e-57 MJ1392 [E] COG0119 Isopropylmalate/homocitrate/citramalate synth... 190 3e-48 MK0391 [E] COG0119 Isopropylmalate/homocitrate/citramalate synth... 189 7e-48 SSO2407 [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 181 2e-45 >alr1407 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Length = 359 Score = 219 bits (559), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 0/336 (0%), Positives = 0/336 (0%), Gaps = 3/336 (0%) Query: 1 VEQAEVGIPAMGTREQEDIAAIAELNLPMKLMTWNRSLPGDIDKARATGVNWSHVSIPVS 60 + + +A + E L + S + Sbjct: 39 GHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTA 98 Query: 61 EIQLQGKLGLSTDEGLNKLLGAAEYALSLGMTVSVGMEDSSRADMGFLIRLVNSLHREGI 120 + L + + + + + + V Sbjct: 99 VHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVS 158 Query: 121 CRFRYADTVSAHHPGQIAERVSTLLGEVPGDVELEVHCHNDFGLACANXXXXXXXXXXXX 180 R + Q+A + G+ ++ Sbjct: 159 HFARADEPKCGATEKQLAIFNTFCEGKP--GQRSIAASGGILLWPQSHFDWVRPGIILYG 216 Query: 181 XXXXXXIGERTGNAAMEEVVMAWHHLYGGDSSVRFDLLKGLADKVIAASGRSVGDAKPIV 240 + + + + + G + V Sbjct: 217 VSPLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD 276 Query: 241 GQLAFTHESGIHVDGLMKERATYQTFDPSEVGRSHRFVLGKHSGTGGVAHVLEQLGLAIT 300 G + + + K + + Sbjct: 277 GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERIAE 336 Query: 301 PDTAGRLLVRVREYAESHKGTVPEYMLVQWLMEEQQ 336 Sbjct: 337 - 371 >alr2968 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Length = 359 Score = 219 bits (558), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 0/335 (0%), Positives = 0/335 (0%), Gaps = 2/335 (0%) Query: 1 VEQAEVGIPAMGTREQEDIAAIAELNLPMKLMTWNRSLPGDIDKARATGVNWSHVSIPVS 60 + + +A + E L + S + Sbjct: 39 GHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTA 98 Query: 61 EIQLQGKLGLSTDEGLNKLLGAAEYALSLGMTVSVGMEDSSRADMGFLIRLVNSLHREGI 120 + L + + + + + + V Sbjct: 99 VHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVS 158 Query: 121 CRFRYADTVSAHHPGQIAERVSTLLGEVPGDVELEVHCHNDFGLACANXXXXXXXXXXXX 180 R + Q+A + G+ ++ Sbjct: 159 HFARADEPKCGATEKQLAIFNTFCEGKPG--QRSIAASGGILLWPQSHFDWVRPGIILYG 216 Query: 181 XXXXXXIGERTGNAAMEEVVMAWHHLYGGDSSVRFDLLKGLADKVIAASGRSVGDAKPIV 240 + + + + + G + V Sbjct: 217 VSPLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD 276 Query: 241 GQLAFTHESGIHVDGLMKERATYQTFDPSEVGRSHRFVLGKHSGTGGVAHVLEQLGLAIT 300 G + + + K + + Sbjct: 277 GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERIAE 336 Query: 301 PDTAGRLLVRVREYAESHKGTVPEYMLVQWLMEEQ 335 Sbjct: 337 371 >MJ1392 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Length = 491 Score = 190 bits (482), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 112/316 (35%), Positives = 165/316 (52%), Gaps = 2/316 (0%) Query: 1 VEQAEVGIPAMGTREQEDIAAIAELNLPMKLMTWNRSLPGDIDKARATGVNWSHVSIPVS 60 V+ E G E+E I I + L ++ ++ R+LP DID A V+ H+ +P S Sbjct: 38 VDVIEAGSAITSKGEREGIKLITKEGLNAEICSFVRALPVDIDAALECDVDSVHLVVPTS 97 Query: 61 EIQLQGKLGLSTDEGLNKLLGAAEYALSLGMTVSVGMEDSSRADMGFLIRLVNSLHREGI 120 I ++ KL + DE L L A EYA G+ V + ED++R+D+ FLI+L N + G Sbjct: 98 PIHMKYKLRKTEDEVLETALKAVEYAKEHGLIVELSAEDATRSDVNFLIKLFNEGEKVGA 157 Query: 121 CRFRYADTVSAHHPGQIAERVSTLLGEVPGDVELEVHCHNDFGLACANXXXXXXXXXXXX 180 R DTV P + E + V ++ + VHCHNDFG+A AN Sbjct: 158 DRVCVCDTVGVLTPQKSQELFKKITENV--NLPVSVHCHNDFGMATANTCSAVLGGAVQC 215 Query: 181 XXXXXXIGERTGNAAMEEVVMAWHHLYGGDSSVRFDLLKGLADKVIAASGRSVGDAKPIV 240 IGER GNA++EEVV A LYG D+ ++ + L ++ V V K IV Sbjct: 216 HVTVNGIGERAGNASLEEVVAALKILYGYDTKIKMEKLYEVSRIVSRLMKLPVPPNKAIV 275 Query: 241 GQLAFTHESGIHVDGLMKERATYQTFDPSEVGRSHRFVLGKHSGTGGVAHVLEQLGLAIT 300 G AF HE+GIHVDGL+K TY+ P VG R +LGKHSG + + L+ +G+ ++ Sbjct: 276 GDNAFAHEAGIHVDGLIKNTETYEPIKPEMVGNRRRIILGKHSGRKALKYKLDLMGINVS 335 Query: 301 PDTAGRLLVRVREYAE 316 + ++ RV+E+ + Sbjct: 336 DEQLNKIYERVKEFGD 351 >MK0391 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Length = 499 Score = 189 bits (479), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 114/319 (35%), Positives = 165/319 (51%), Gaps = 2/319 (0%) Query: 1 VEQAEVGIPAMGTREQEDIAAIAELNLPMKLMTWNRSLPGDIDKARATGVNWSHVSIPVS 60 V+ E G P E E + AIA L ++ R + GDID A V+ HV I S Sbjct: 40 VDTIEAGFPVASEGEFEAVRAIAGEELDAEICGLARCVKGDIDAAIDADVDCVHVFIATS 99 Query: 61 EIQLQGKLGLSTDEGLNKLLGAAEYALSLGMTVSVGMEDSSRADMGFLIRLVNSLHREGI 120 +I L+ KL +S +E L + + EYA G+TV ED++R D +L+ + + G Sbjct: 100 DIHLRYKLEMSREEALERAIEGVEYASDHGVTVEFSAEDATRTDRDYLLEVYKATVEAGA 159 Query: 121 CRFRYADTVSAHHPGQIAERVSTLLGEVPGDVELEVHCHNDFGLACANXXXXXXXXXXXX 180 R DTV P ++ + ++ V DV + VHCHNDFG+A AN Sbjct: 160 DRVNVPDTVGVMTPPEMYRLTAEVVDAV--DVPVSVHCHNDFGMAVANSLAAVEAGAEQV 217 Query: 181 XXXXXXIGERTGNAAMEEVVMAWHHLYGGDSSVRFDLLKGLADKVIAASGRSVGDAKPIV 240 IGER GNA++E+VVMA LY + VR ++L L+ V +G V PIV Sbjct: 218 HVTVNGIGERAGNASLEQVVMALKALYDIELDVRTEMLVELSRLVERLTGVVVPPNTPIV 277 Query: 241 GQLAFTHESGIHVDGLMKERATYQTFDPSEVGRSHRFVLGKHSGTGGVAHVLEQLGLAIT 300 G+ AF HESGIH G++K+ TY+ P +VG R VLGKH+G + LE++G+ +T Sbjct: 278 GENAFAHESGIHSHGVIKKAETYEPIRPEDVGHRRRIVLGKHAGRHAIKKKLEEMGIEVT 337 Query: 301 PDTAGRLLVRVREYAESHK 319 + ++ RV+E + K Sbjct: 338 EEQLDEIVRRVKELGDKGK 356 >SSO2407 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Length = 347 Score = 181 bits (459), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 112/320 (35%), Positives = 168/320 (52%), Gaps = 5/320 (1%) Query: 1 VEQAEVGIPAMGTREQEDIAA---IAELNLPMKLMTWNRSLPGDIDKARATGVNWSHVSI 57 V+ E G PA + E E IA E+ ++++ +RS DIDK +TG++ H+ I Sbjct: 11 VDVIEAGFPA--SSEGEFIATRKIFEEIGDQVEVIGLSRSNKNDIDKTISTGISSIHLFI 68 Query: 58 PVSEIQLQGKLGLSTDEGLNKLLGAAEYALSLGMTVSVGMEDSSRADMGFLIRLVNSLHR 117 SE+ ++ KL ++ +E LNK+ + +YA GM V ED++R + FL + + Sbjct: 69 ATSELHMKYKLKMTKEEVLNKIYESVKYAKDHGMIVEFSPEDATRTEEDFLFTAIRTAIE 128 Query: 118 EGICRFRYADTVSAHHPGQIAERVSTLLGEVPGDVELEVHCHNDFGLACANXXXXXXXXX 177 G R DTV HP + + + ++ V + + VHCHNDFGLA AN Sbjct: 129 AGADRINIPDTVGTMHPFKYYDMIKKIVNFVGERIIVSVHCHNDFGLATANSLAGVYAGA 188 Query: 178 XXXXXXXXXIGERTGNAAMEEVVMAWHHLYGGDSSVRFDLLKGLADKVIAASGRSVGDAK 237 IGER GNA++EEVVM L +++V+ L ++ V +G V K Sbjct: 189 RQAHVTVNGIGERAGNASLEEVVMGIKKLLNYETNVKTWKLYEVSRFVAEMTGVPVPYFK 248 Query: 238 PIVGQLAFTHESGIHVDGLMKERATYQTFDPSEVGRSHRFVLGKHSGTGGVAHVLEQLGL 297 IVG AF HESGIHV G+++ TY+ P EVG R LGKHSG G+ +LE+ G+ Sbjct: 249 AIVGDNAFGHESGIHVHGVIENPFTYEPISPEEVGNFRRLALGKHSGIHGLRKLLEEQGI 308 Query: 298 AITPDTAGRLLVRVREYAES 317 ++ D +L +++ AES Sbjct: 309 YLSDDKLKIVLAEIKKLAES 328 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,513,421 Number of extensions: 1130501 Number of successful extensions: 4191 Number of sequences better than 1.0e-05: 69 Number of HSP's gapped: 4041 Number of HSP's successfully gapped: 69 Length of query: 342 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 235 Effective length of database: 26,037,693 Effective search space: 6118857855 Effective search space used: 6118857855 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)