BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0151.0011.p (250 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1432 [H] COG0476 Dinucleotide-utilizing enzymes involved in m... 138 1e-32 PA2103_1 [H] COG0476 Dinucleotide-utilizing enzymes involved in ... 123 3e-28 MT3198_1 [H] COG0476 Dinucleotide-utilizing enzymes involved in ... 100 4e-21 AF0553 [H] COG0476 Dinucleotide-utilizing enzymes involved in mo... 99 7e-21 mll5577 [H] COG0476 Dinucleotide-utilizing enzymes involved in m... 97 2e-20 >all1432 [H] COG0476 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2 Length = 211 Score = 138 bits (347), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 0/229 (0%), Positives = 0/229 (0%), Gaps = 5/229 (2%) Query: 20 LGEEGQRALKEATVMVXXXXXXXXXXXXXXXXXXXXXXILAHEGIVVPPDLNRQILMDTD 79 E R + ++ Sbjct: 6 RLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAML 65 Query: 80 HLGMERMSTAIAQLKRLNPHVEIEGHNARIEIPLARPWVQDADIVIDARYDFPERYALNR 139 LG+E + T I + N E RI + V + ++A + A Sbjct: 66 ELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANVV 125 Query: 140 LCVDTGTPMVEAAMYGFEISLTTVVPGVTPCLECLYPDVAPLWEPLGFPVLGATSGIAGC 199 L G P + + F + T + + + G Sbjct: 126 LNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKL-LKVVPAEFKVDCHHWLILHGR 184 Query: 200 LAALEAVKWITGVGTTYAGVMHR--FSSLDFACYSVHISRNPKCACCGE 246 + Sbjct: 185 YTCIARKPRCGSCIIEDLCEYKEKV-- 231 >PA2103_1 [H] COG0476 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2 Length = 266 Score = 123 bits (308), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 74/245 (30%), Positives = 121/245 (49%), Gaps = 9/245 (3%) Query: 9 ELERYARQLKL--LGEEGQRALKEATVMVXXXXXXXXXXXXXXXXXXXXXXILAHEGIVV 66 E++RY R + L +G EGQ+ LK+A V++ L +V Sbjct: 17 EVQRYVRHITLPGVGREGQQRLKQARVLIIGTGGLGSPACLYLAAAGVGTIGLVDFDVVE 76 Query: 67 PPDLNRQILMDTDHLGMERMSTAIAQLKRLNPHVEIEGHNARIEIPLARPWVQDADIVID 126 +L RQ++ LGM ++ +A +++ LNP++E+ H I+ A+ +Q D+V+D Sbjct: 77 SSNLQRQVVHGYSTLGMPKVESAQRRMQDLNPYIEVVTHRYAIDADNAQSLIQSYDLVLD 136 Query: 127 ARYDFPERYALNRLCVDTGTPMVEAAMYGFEISLTTVVPGVTPCLECLYPD-----VAPL 181 +F RY +N +C G P+V A+Y FE ++ PC +CL+P+ +AP Sbjct: 137 GTDNFATRYLVNAVCAKLGKPLVFGAIYRFEGQVSVFNLEGGPCYQCLFPNNPPPEMAPS 196 Query: 182 WEPLGFPVLGATSGIAGCLAALEAVKWITGVGTTYAGVMHRFSSLDFACYSVHISRNPKC 241 G V+G G+ G L A EAVK + G+G + +G + RF +L V +R C Sbjct: 197 CNAGG--VIGVLPGVIGLLQATEAVKALVGLGKSLSGRLLRFDALLMHFNEVKFNRRDDC 254 Query: 242 ACCGE 246 CG+ Sbjct: 255 PACGK 259 >MT3198_1 [H] COG0476 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2 Length = 274 Score = 99.8 bits (247), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 69/248 (27%), Positives = 109/248 (43%), Gaps = 9/248 (3%) Query: 9 ELERYARQLKL--LGEEGQRALKEATVMVXXXXXXXXXXXXXXXXXXXXXXILAHEGIVV 66 E+ RY+R L + +G GQ+ LK+A V+ + V Sbjct: 18 EVRRYSRHLIIPDIGVNGQQRLKDARVLCIGAGGLGSPALLYLAAAGVGTIGIIDGDHVD 77 Query: 67 PPDLNRQILMDTDHLGMERMSTAIAQLKRLNPHVEIEGHNARIEIPLARPWVQDADIVID 126 +L RQI+ T +G ++ +A + +NPHV + + + A D D+++D Sbjct: 78 ESNLQRQIIHGTSDVGRPKVESAAEAVAEINPHVRVTQYREMLTHDNALEIFGDHDLIVD 137 Query: 127 ARYDFPERYALNRLCVDTGTPMVEAAMYGFEISLTTVVPGVTPCLECLY-----PDVAPL 181 +F RY +N V G P V ++Y F + PG PC CL+ P + P Sbjct: 138 GTDNFTTRYLINDAAVLAGKPYVWGSIYRFNGQTSVFWPGRGPCYRCLHPAPPPPGLVPS 197 Query: 182 WEPLGFPVLGATSGIAGCLAALEAVKWITGVGTTYAGVMHRFSSLDFACYSVHISRNPKC 241 G VLGA + E +K +TGVGT G + + +LD + + I++NP C Sbjct: 198 CAEGG--VLGAICATIASIQVTEVLKLLTGVGTPLVGRLLMYEALDATYHQIRIAKNPDC 255 Query: 242 ACCGEGGT 249 A CG+ T Sbjct: 256 AICGDAPT 263 >AF0553 [H] COG0476 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2 Length = 267 Score = 99.0 bits (245), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 71/254 (27%), Positives = 115/254 (45%), Gaps = 24/254 (9%) Query: 9 ELERYARQLKL--LGEEGQRALKEATVMVXXXXXXXXXXXXXXXXXXXXXXILAHEGIVV 66 +++RY RQ+ + +G GQ L +A V+V +A V Sbjct: 6 QVKRYGRQILIPEIGGRGQEKLLKAKVLVVGAGGLGSPAIEFLAAAGVGRIGIADGDEVD 65 Query: 67 PPDLNRQILMDTDHLGMERMSTAIAQLKRLNPHVEIEGHNARIEIPLARPWVQDADIVID 126 +L+RQ + +LG+ + +A + +++LNP VE++ + + AR + D+V+D Sbjct: 66 ITNLHRQTIH-AGNLGVNKAESAASFVEKLNPDVEVDVYPFHLSAENAREVISKYDVVLD 124 Query: 127 ARYDFPERYALNRLCVDTGTPMVEAAMYGFEISLTTVVPGVTPCLECLYPDVAPLWEPLG 186 F R+ +N CV P V AA+ FE + TV+P T C C P+ P P Sbjct: 125 CTDSFSSRFLINDACVLESKPFVHAAVLRFEGQIMTVIPHETACYRCFLPEAPP---PGS 181 Query: 187 FP------VLGATSGIAGCLAALEAVKWITGVGT------TYAGVMHRFSSLDFACYSVH 234 P ++G T+ G L A EA+K I G G YA ++H + +F + Sbjct: 182 VPSCREAGIIGTTTAFFGVLQANEAIKLILGTGELFTNRLLYADILH--NDFNF----IE 235 Query: 235 ISRNPKCACCGEGG 248 + + KC CGE G Sbjct: 236 VKKQEKCRVCGESG 249 >mll5577 [H] COG0476 Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2 Length = 250 Score = 97.4 bits (241), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 72/241 (29%), Positives = 110/241 (45%), Gaps = 12/241 (4%) Query: 9 ELERYARQLKL--LGEEGQRALKEATVMVXXXXXXXXXXXXXXXXXXXXXXILAHEGIVV 66 E+ERYAR + L +G GQ+ LK+A V+V + + V Sbjct: 9 EIERYARHIVLPEVGGAGQQKLKQARVLVIGAGGLGAPVLEYLAAAGVGTLGIVDDDTVS 68 Query: 67 PPDLNRQILMDTDHLGMERMSTAIAQLKRLNPHVEIEGHNARIEIPLARPWVQDADIVID 126 +L RQ++ TD +G + +A A + +NP+ +E H R+ A V DIV+D Sbjct: 69 LSNLQRQVIHATDAVGKLKTDSARAAIAHINPNTAVETHTFRLSADNAPALVTRYDIVVD 128 Query: 127 ARYDFPERYALNRLCVDTGTPMVEAAMYGFEISLTTVVP---GVTPCLECLYPDVAPLWE 183 +F RY + C P+V AA+ F+ S+T + P G Y D+ P Sbjct: 129 GSDNFETRYTVADACASARKPLVHAAVGRFDGSVTVLKPFEVGKDGKPNPGYRDLFPEAP 188 Query: 184 PLGF-------PVLGATSGIAGCLAALEAVKWITGVGTTYAGVMHRFSSLDFACYSVHIS 236 P G VLGA +G+ G L A+EA+K ITG+G G + + +L ++ S Sbjct: 189 PPGLVPSCAEAGVLGALTGVVGTLQAMEAIKLITGIGEPLIGRLLLYDALAARFETIRYS 248 Query: 237 R 237 R Sbjct: 249 R 249 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.139 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,104,827 Number of extensions: 700172 Number of successful extensions: 1785 Number of sequences better than 1.0e-05: 39 Number of HSP's gapped: 1735 Number of HSP's successfully gapped: 39 Length of query: 250 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 146 Effective length of database: 26,383,695 Effective search space: 3852019470 Effective search space used: 3852019470 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)