BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0151.0008.p (442 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1437 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 297 3e-80 mlr5909 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 271 2e-72 SMa0873 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 248 1e-65 CAC0257 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 178 1e-44 MTH1566 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 176 1e-43 >all1437 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 444 Score = 297 bits (760), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 169/431 (39%), Positives = 242/431 (56%), Gaps = 8/431 (1%) Query: 3 VNRRRKPASVNPIKTGQSLGAVLALQGCYRAMPLLHGSQGCSAFPKALLTRHFREPIAVQ 62 V K +VNP+K Q+LGA LA G +PL HGSQGC+AF K +L RHFRE I + Sbjct: 4 VTLPNKSVAVNPLKQSQALGASLAFLGLKGMIPLFHGSQGCTAFAKVVLVRHFREAIPLA 63 Query: 63 TSALQEMDVIFDANRNLEEALNLVLSKHRPDIIGIIGTELTDVAGVDYQSMLKSYKRERN 122 T+A+ E+ I N+E+A+ ++ K P+IIG+ T LT+ G D + LK + Sbjct: 64 TTAMTEVTTILGGEDNIEQAILTLVEKSSPEIIGLCSTGLTETRGDDIERFLKDIRDRHP 123 Query: 123 MRGGLAFSVV-LPDFRGSLESGFSAAVESMIDEMIQQVGHRGSRSVNTRQITLLPGSFLT 181 L PDF+G+L+ GF+AAVES++ E I Q G + + Q+T+L GS T Sbjct: 124 EISHLPIVFAPTPDFKGALQDGFAAAVESIVQE-IPQPG-----TTRSEQVTILAGSAFT 177 Query: 182 PADVMELKEMISSFGFEVIALPDISTSLSGHLLTGFSPLTRGGVPLDSMLQSLQSGLTIA 241 P D+ E+KE++++FG I +PDI SL GHL +S +T G + + + S TIA Sbjct: 178 PGDLQEIKEIVTAFGLVPIFVPDIGASLDGHLDEEYSSVTTSGTTVKQLQEVGCSAFTIA 237 Query: 242 IGGSMERPARRLHNALGTPYKVFTHAMGLKASDELLHFLHQISGEPVPLRYCWQRENLLD 301 +G SM AR L + PY+VF+ GL+ DE + L +S VP +YC QR L D Sbjct: 238 LGESMRGAARILEDRFNIPYEVFSELTGLEPVDEFIQALAILSSNSVPEKYCRQRRQLQD 297 Query: 302 SMLDAHFQFAGMSAIAALEPDHLCSVSGWLEELGVEQKALIASYETPLVAGME-REVWIG 360 +MLD HF F ALEPD L S+ +L+ +G + A + + +PL+ + + V IG Sbjct: 298 AMLDTHFYFGAKRISLALEPDLLWSMVKFLQSMGTQIHAAVTTTRSPLLEQLPIKSVTIG 357 Query: 361 DLDDAEVLGKGADLWISNSHGIAGAQRIAAAFMPAGFPVWNELGAYMSVSVGYRGAMEWS 420 DL+D E L +DL I NS+ A A+R++ G P+++ LG VGYRG+ME Sbjct: 358 DLEDFEQLAVESDLLIGNSNLAAIAKRLSIPHYRLGIPIYDRLGNGHYTKVGYRGSMEVL 417 Query: 421 NKAGNLLMQRE 431 GNL + E Sbjct: 418 FGIGNLFIDAE 428 >mlr5909 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 460 Score = 271 bits (693), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 151/427 (35%), Positives = 229/427 (53%), Gaps = 8/427 (1%) Query: 5 RRRKPASVNPIKTGQSLGAVLALQGCYRAMPLLHGSQGCSAFPKALLTRHFREPIAVQTS 64 R+ K A+VNP+K+ Q LGA A G AMPL HGSQGC++F L RHF+E I +QT+ Sbjct: 6 RKIKSAAVNPLKSSQPLGAAFAFLGVEGAMPLFHGSQGCTSFALVLFVRHFKEAIPLQTT 65 Query: 65 ALQEMDVIFDANRNLEEALNLVLSKHRPDIIGIIGTELTDVAGVDYQSMLKSYKRE--RN 122 A+ E+ I +LEEA+ + ++ +P +IG+ T L + G D S + + K + Sbjct: 66 AMDEVATILGGADHLEEAILNLKTRTKPKLIGVCTTALVETRGEDCASDIANVKLKHVEE 125 Query: 123 MRGGLAFSVVLPDFRGSLESGFSAAVESMIDEMIQQVGHRGSRSVNTRQITLLPGSFLTP 182 + G PDF G++E G++ AV +MI+ + + G R+ ++I +LPG LT Sbjct: 126 LAGTEVVLANTPDFDGAIEEGWAKAVAAMIEGITRS----GERTRQPKKIAILPGCNLTV 181 Query: 183 ADVMELKEMISSFGFEVIALPDISTSLSGHLLTGFSPLTRGGVPLDSMLQSLQSGLTIAI 242 ADV L++M+ SFG + + LPD+S SL G + + T GG ++ + + + I I Sbjct: 182 ADVEHLRDMVESFGLKPVILPDVSGSLDGTVPDRWVTTTYGGTSVEEIRELGTAAQCIVI 241 Query: 243 GGSMERPARRLHNALGTPYKVFTHAMGLKASDELLHFLHQISGEPVPLRYCWQRENLLDS 302 G M PA+ LH G PY VF GLKA D + L +SG VP R R L D+ Sbjct: 242 GEHMRHPAKTLHGLTGVPYAVFQSLTGLKAVDRFVSLLSAVSGAAVPDRVRRHRAQLEDA 301 Query: 303 MLDAHFQFAGMSAIAALEPDHLCSVSGWLEELGVEQKALIASYETPLVAGMEREVW--IG 360 +LD HF F G A EPD L ++ + +G + A + + + + W IG Sbjct: 302 LLDGHFHFGGKKIAIAAEPDQLYQLATFFTGMGCDIAAAVTTTDMSKILQKVPAEWVQIG 361 Query: 361 DLDDAEVLGKGADLWISNSHGIAGAQRIAAAFMPAGFPVWNELGAYMSVSVGYRGAMEWS 420 DL D E L GADL +++SHG ++R+ M GFP+++ LG+ +++ YRG + Sbjct: 362 DLGDLEALAAGADLLVTHSHGRQASRRLEIPLMRVGFPIFDRLGSQHKLTILYRGTRDLI 421 Query: 421 NKAGNLL 427 N+ Sbjct: 422 FDVANIF 428 >SMa0873 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 441 Score = 248 bits (634), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 150/430 (34%), Positives = 234/430 (54%), Gaps = 15/430 (3%) Query: 6 RRKPASVNPIKTGQSLGAVLALQGCYRAMPLLHGSQGCSAFPKALLTRHFREPIAVQTSA 65 + K A++NP+K+ Q LG LA G A+PL HGSQGC++F LL RHF+E I +QT+A Sbjct: 7 QTKWATINPLKSSQPLGGALAFLGVGGAIPLFHGSQGCTSFALVLLVRHFKEAIPLQTTA 66 Query: 66 LQEMDVIFDANRNLEEALNLVLSKHRPDIIGIIGTELTDVAGVDYQSMLKSYKRER--NM 123 + ++ ++ +LE+A+ + + +P +IGI T L + G D L S K ER + Sbjct: 67 MDDVAIVLGGAGHLEQAILNLKIRAKPKLIGICTTALVETRGEDLAGDLASIKLERAEEL 126 Query: 124 RGGLAFSVVLPDFRGSLESGFSAAVESMIDEMIQQVGHRGSRSVNTRQITLLPGSFLTPA 183 G PDF G++E G++ AV +MI + I ++G + +S R I +LPG LT A Sbjct: 127 TGTDVVLANTPDFDGAMEEGWAKAVTAMI-KAITRIGEQERQS---RTIAILPGWNLTIA 182 Query: 184 DVMELKEMISSFGFEVIALPDISTSLSGHLLTG-FSPLTRGGVPLDSMLQSLQSGLTIAI 242 D+ +L++++ SFG + I LPD+S SL G + + P T GG+ ++ + + + IAI Sbjct: 183 DIEQLRDIVESFGLKPIILPDLSGSLDGIVPDDRWVPTTYGGISVEEIRELGTAAQCIAI 242 Query: 243 GGSMERPARRLHNALGTPYKVFTHAMGLKASDELLHFLHQISGEPVPLRYCWQRENLLDS 302 G M PA + G PY +F GL A D + L ISG P P + +R L D+ Sbjct: 243 GEHMRGPAEEMKTLTGVPYVLFQSLTGLNAVDRFVSLLSSISGRPAPAKVRRRRAQLQDA 302 Query: 303 MLDAHFQFAGMSAIAALEPDHLCSVSGWLEELGVEQKALIASYETPLVAGMEREV----- 357 +LD HF AG A EPD L ++ + LG E ++A+ T + + +V Sbjct: 303 LLDGHFHSAGKKIAIAAEPDQLYQLATFFICLGAE---IVAAVTTKGASKILHKVPVEII 359 Query: 358 WIGDLDDAEVLGKGADLWISNSHGIAGAQRIAAAFMPAGFPVWNELGAYMSVSVGYRGAM 417 +GDL D E L ADL +++SHG + R+ M GFPV+++LG+ +++ Y G Sbjct: 360 QVGDLGDLESLATHADLLVTHSHGQHASARLGTPLMRVGFPVFDQLGSQHKLTILYHGTR 419 Query: 418 EWSNKAGNLL 427 + + N+ Sbjct: 420 DLIFEVSNIF 429 >CAC0257 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 454 Score = 178 bits (452), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 125/443 (28%), Positives = 211/443 (47%), Gaps = 31/443 (6%) Query: 6 RRKPASVNPIKTGQSLGAVLALQGCYRAMPLLHGSQGCSAFPKALLTRHFREPIAVQTSA 65 RK +NP +T Q +GA+ A G + +P HGSQGC ++ + LTRHF++PI TS+ Sbjct: 11 ERKALRINPARTCQPIGAMYAALGIHNCLPHSHGSQGCCSYHRMQLTRHFKDPIMASTSS 70 Query: 66 LQEMDVIFDANRNLEEALNLVLSKHRPDIIGIIGTELTDVAGVDYQSMLKSYKRERNM-R 124 E +F N A+ + S + PDI+ + T L++ G D L SY + + Sbjct: 71 FNEGASVFGGRANFNTAIKNIFSIYNPDIVAVHTTCLSETLGDD----LVSYIADSEVPE 126 Query: 125 GGLAFSVVLPDFRGSLESGFSAAVESMIDEMIQQVGHRGSRSVNTRQITLLPGSFLTPAD 184 G + P + GS +GFS+ V++M+ + + ++ + + +LPG F+ PAD Sbjct: 127 GKIVIHANTPSYVGSHITGFSSMVKAMVTYLSENTKNKNGK------VNVLPG-FVGPAD 179 Query: 185 VMELKEMISSFGFEVIALPDISTSLSGHLLTGFSPLTRGGVPLDSMLQSLQSGLTIAIGG 244 + ELK++ I +PD S + + + +GG +D + + S T A+G Sbjct: 180 MRELKKIFKCMKVPFIMMPDTSGVVDSPMTGEYKMYPKGGTTIDELRDAGNSDYTFALGS 239 Query: 245 -SMERPARRLHNALGTPYKVFTHAMGLKASDELLHFLHQISGEPVPLRYCWQRENLLDSM 303 + E A L P+ V +G+ A+D + L +I+G+ VP +R L+D M Sbjct: 240 LASEAAAIELEKKCKVPFSVLNTPIGIDATDRFIMELSKITGKKVPYEIEEERGQLVDLM 299 Query: 304 LDAHFQFAGMSAIAALEPDHLCSVSGWLEELGVEQKALIA-----SYETPLVAGMEREVW 358 LD++ F G + +PD + +++ ++ LG+ K +I +E + +E Sbjct: 300 LDSYQYFHGKKVAISGDPDTVIALTEFVISLGMNPKYVITGTKGEKFEKTINKMLEDNGI 359 Query: 359 IG-------DLDDAE--VLGKGADLWISNSH--GIAGAQRIAAAFMPAGFPVWNELGAYM 407 G DL + + + DL I NSH IA A+ I + GFPV + G Sbjct: 360 EGSKVKSFSDLFELHQWIKNEPVDLVIGNSHMKFIARAEDI--PLVRFGFPVMDRYGHSY 417 Query: 408 SVSVGYRGAMEWSNKAGNLLMQR 430 VGY+GAM + +++R Sbjct: 418 IPKVGYKGAMNLLTSMCDAILER 440 >MTH1566 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 268 Score = 176 bits (445), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 0/444 (0%), Positives = 0/444 (0%), Gaps = 26/444 (5%) Query: 11 SVNPIKTGQSLGAVLALQGCYRAMPLLHGSQGCSAFPKALLTRHFREPIAVQTSALQEMD 70 I + Y+ + + + + L MD Sbjct: 6 LSIFIPDSLTAETGDLKIKTYKVVLIARAASIFGVKRIVIYHDDADGEARFIRDILTYMD 65 Query: 71 VIFDANRNLEEALNLVLSKHRPDIIGIIGTELTDVAGVDYQSMLKSYKRERNMRGGLAFS 130 R + + + + +Y+ L + ++ + Sbjct: 66 TPQYLRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKGTLVDIGAD 125 Query: 131 VVLPDFRGSLESGFSAAVESMIDEMIQQVGHRGSRSVNTRQITLLPGSFLTPADVMELKE 190 + ++ + G + L E Sbjct: 126 K--LALCREKLTVNRIMSFRVVRLG------KEILIEPDEPEDRYWG-YEVLDTRRNLAE 176 Query: 191 MISSFGFEVIALPDISTSLSGHLLTGFSPLTRGGVPLDSMLQSLQSGLTIAIGGSMERPA 250 + + G +V+ + S +L RG + GL Sbjct: 177 SLKTVGADVVVATSRNASPITSILDEVKTRMRGAREAAILFGGPYKGLPEIDADI--WVN 234 Query: 251 RRLHNALGTPYKVFTHAMGLKASDELLHFLHQISGEPVPLRYCWQRENLLDSMLDAHFQF 310 Sbjct: 235 294 Query: 311 AGMSAIAALEPDHLCSVSGWLEELGVEQKALIASYETPL----VAGMEREVWI------- 359 Sbjct: 295 354 Query: 360 GDLDDAE--VLGKGADLWISNSHGIAGAQRIAAAFMPAGFPVWNELGAYMSVSVGYRGAM 417 Sbjct: 355 414 Query: 418 EWSNKAGNLLMQR--EAGHRESSV 439 Sbjct: 415 438 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,076,305 Number of extensions: 1439196 Number of successful extensions: 5568 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 5537 Number of HSP's successfully gapped: 16 Length of query: 442 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 333 Effective length of database: 25,807,025 Effective search space: 8593739325 Effective search space used: 8593739325 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)