BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0151.0007.p (468 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1438 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 524 e-148 mlr5908 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 452 e-127 SMa0830 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 437 e-122 MTH1565 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 311 2e-84 CAC0258 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 303 5e-82 >all1438 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 480 Score = 524 bits (1350), Expect = e-148, Method: Compositional matrix adjust. Identities = 246/430 (57%), Positives = 323/430 (75%), Gaps = 1/430 (0%) Query: 34 KVRPCPR-PKPGEAAGGCSFDGAQVTLLPIMDAAHLVHGPIACAGNSWESRGTLSSGPSL 92 K + C + +PG A GGC+FDGA ++L+PI+DAAHLVHGPIACAGNSW SRG+LSSGP L Sbjct: 28 KNKSCSQQAQPGAAQGGCAFDGAMISLVPIVDAAHLVHGPIACAGNSWGSRGSLSSGPQL 87 Query: 93 SQYGFATDLTDSDIIFGGEKKLKDSIDYIAGRFAPPAIFVYSTCVTALTGEDMDAVCKTA 152 + GF TD++++D+IFGGEKKL +I I R+ P A+FVY+TCVTAL G+D+DAVCKTA Sbjct: 88 YKMGFTTDMSENDVIFGGEKKLYKAILEIHKRYNPSAVFVYATCVTALIGDDIDAVCKTA 147 Query: 153 AERLGIPVIPVNSPGFVGSKNLGNRLAGDALLQYVIGTGEPSPDMPMGINLIGEYNIAGE 212 AE++G PVIPV +PGF+GSKNLGNR G++LL YV+GT EP P INLIGEYNIAGE Sbjct: 148 AEKIGTPVIPVIAPGFIGSKNLGNRFGGESLLDYVVGTAEPEYTTPYDINLIGEYNIAGE 207 Query: 213 MWDIEKLMNSAGISVTSRITGDARYKEVTWAHRAKVNMVVCSRALLGLAREMESRYGIPY 272 MW + L+ GI V S+ITGDAR++E+ +AHRAK+N+++CSRALL +AR+ME YGIPY Sbjct: 208 MWGVLPLLEKLGIRVLSKITGDARFEEIRYAHRAKLNVMICSRALLNMARKMEENYGIPY 267 Query: 273 FEGSFYGAKETTYSLRQMAYLMNDRDMERRVDRLTEREEARLSQELRPYRKILKGKKAVL 332 E SFYG + LR +A + D D++ R ++L E A L L PYR LKGK+ VL Sbjct: 268 IEESFYGIDDMNRCLRNIAAKLGDPDLQARTEKLIAEETAALDLALAPYRARLKGKRVVL 327 Query: 333 YTGGVKSWSVISALKELGVQVVGVGTNKSTEDDVRRIADRVGDDTEYIPEGGAGRILKTV 392 YTGGVKSWS+ISA K+LG++VV T KSTE+D +I +G D + +G A +L+ V Sbjct: 328 YTGGVKSWSIISAAKDLGIEVVATSTRKSTEEDKAKIKRLLGADGIMLEKGNAKELLQLV 387 Query: 393 RERKADIMIAGGRNMYVAMKEQIPFIDINQERHKAYAGYEGLLRLAKELTYSLANPIWKL 452 ++ +AD++IAGGRN Y A+K +IPF+DINQERH YAGY G++ +A+EL +L +PIW+ Sbjct: 388 KDTQADMLIAGGRNQYTALKARIPFLDINQERHHPYAGYVGMIEMARELYEALYSPIWEQ 447 Query: 453 AAGPAPWDKE 462 PAPWD++ Sbjct: 448 IRKPAPWDED 457 >mlr5908 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 496 Score = 452 bits (1162), Expect = e-127, Method: Compositional matrix adjust. Identities = 220/452 (48%), Positives = 308/452 (68%), Gaps = 11/452 (2%) Query: 20 KDEFDSGACGS-----LAPKVRPCPRP-KPGEAAGGCSFDGAQVTLLPIMDAAHLVHGPI 73 +D FD AC + + C +P PG +AGGC+FDGA++ L PI D AHLVH P+ Sbjct: 9 QDVFDEPACDTNRGKDAKARKEGCSKPLTPGASAGGCAFDGAKIVLQPITDVAHLVHAPL 68 Query: 74 ACAGNSWESRGTLSSGPSLSQYGFATDLTDSDIIFG-GEKKLKDSIDYIAGRFAPPAIFV 132 AC GNSW++RG SSGP+L + F TDLT+ DI+ G GE+KL +I I +APPA+FV Sbjct: 69 ACEGNSWDNRGAASSGPTLWRTSFTTDLTELDIVMGQGERKLFKAIREIKETYAPPAVFV 128 Query: 133 YSTCVTALTGEDMDAVCKTAAERLGIPVIPVNSPGFVGSKNLGNRLAGDALLQYVIGTGE 192 YSTCVTAL G+D++AVCK AAE+ G+ V+P+N+PG GSKNLGN+LA +ALL +VIGT E Sbjct: 129 YSTCVTALIGDDIEAVCKRAAEKFGLAVVPINAPGLAGSKNLGNKLAAEALLDHVIGTVE 188 Query: 193 PSPDMPMGINLIGEYNIAGEMWDIEKLMNSAGISVTSRITGDARYKEVTWAHRAKVNMVV 252 P P IN++GE+N++GE W ++ L++ GI V + I GDARY +V AHRA+ M+V Sbjct: 189 PDDAGPYDINILGEFNLSGEFWLVKPLLDRLGIRVRACIPGDARYIDVASAHRARAAMMV 248 Query: 253 CSRALLGLAREMESRYGIPYFEGSFYGAKETTYSLRQMAYLM----NDRDMERRVDRLTE 308 CS AL+ LAR+ME R+ IPYFEGSFYG +T+ +LR +A L+ D ++ R + L Sbjct: 249 CSSALINLARKMEERWDIPYFEGSFYGITDTSEALRNIAELLVRKGADAEILDRTETLIA 308 Query: 309 REEARLSQELRPYRKILKGKKAVLYTGGVKSWSVISALKELGVQVVGVGTNKSTEDDVRR 368 +EA ++L YR+ L+GK+ +L TGGVKSWSV+ AL E+G+++VG KST +D R Sbjct: 309 EQEAIAWKKLEAYRRRLQGKRVLLNTGGVKSWSVVHALMEIGMEIVGTSVKKSTVEDKER 368 Query: 369 IADRVGDDTEYIPEGGAGRILKTVRERKADIMIAGGRNMYVAMKEQIPFIDINQERHKAY 428 I + D+ + A + + + KADIM++GGR +VA+K + P++DINQER Y Sbjct: 369 IKRILKDENHMFEQMAARDLYAMLSKHKADIMLSGGRTQFVALKAKTPWLDINQERQHPY 428 Query: 429 AGYEGLLRLAKELTYSLANPIWKLAAGPAPWD 460 AGY+G++ L +++ ++ NPIW PAPWD Sbjct: 429 AGYDGMVELVRQIDLAIHNPIWGQVREPAPWD 460 >SMa0830 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 476 Score = 437 bits (1124), Expect = e-122, Method: Compositional matrix adjust. Identities = 207/425 (48%), Positives = 295/425 (69%), Gaps = 5/425 (1%) Query: 41 PKPGEAAGGCSFDGAQVTLLPIMDAAHLVHGPIACAGNSWESRGTLSSGPSLSQYGFATD 100 P PG AAGGC+FDGA+V L PI + AHL+H P+AC GNSW++RGT SS L + F TD Sbjct: 36 PMPGAAAGGCAFDGAKVALQPITNVAHLIHAPLACEGNSWDNRGTASSSHMLWRTSFTTD 95 Query: 101 LTDSDIIFG-GEKKLKDSIDYIAGRFAPPAIFVYSTCVTALTGEDMDAVCKTAAERLGIP 159 +T+ D++ G E+KL +I I +AP A+FVY+TCVTAL G+D+DAVC+ AAE+ G+P Sbjct: 96 VTEFDVVMGHSERKLFKAIREINEAYAPAAVFVYATCVTALIGDDIDAVCRRAAEKFGLP 155 Query: 160 VIPVNSPGFVGSKNLGNRLAGDALLQYVIGTGEPSPDMPMGINLIGEYNIAGEMWDIEKL 219 V+PVN+PGFVGSKNLGN+LAG+ALL +VIGT EP IN++GE+N++GE W + L Sbjct: 156 VVPVNAPGFVGSKNLGNKLAGEALLDHVIGTVEPDDARSSDINILGEFNLSGEFWQVRPL 215 Query: 220 MNSAGISVTSRITGDARYKEVTWAHRAKVNMVVCSRALLGLAREMESRYGIPYFEGSFYG 279 ++ G+ V + I GD+RY ++ AHRA+ M+VCS AL+ LAR+M R+ IP+FEGSFYG Sbjct: 216 LDKLGVRVRACIPGDSRYLDIATAHRARAAMMVCSTALINLARKMLERWDIPFFEGSFYG 275 Query: 280 AKETTYSLRQMAYLMNDR----DMERRVDRLTEREEARLSQELRPYRKILKGKKAVLYTG 335 +T+ +LRQ+A L+ + D+ R + L EEAR + L YR L+GK+ +L TG Sbjct: 276 ITDTSEALRQIAGLLVKQGAGPDLISRTEALIVEEEARAWRRLEVYRPRLQGKRVLLNTG 335 Query: 336 GVKSWSVISALKELGVQVVGVGTNKSTEDDVRRIADRVGDDTEYIPEGGAGRILKTVRER 395 GVKSWSV AL E+G+++VG KST++D R+ + +D+ + + + Sbjct: 336 GVKSWSVAHALMEIGLEIVGTSIKKSTDNDKERLKQMLTNDSRMSGATTPRELYSALSDH 395 Query: 396 KADIMIAGGRNMYVAMKEQIPFIDINQERHKAYAGYEGLLRLAKELTYSLANPIWKLAAG 455 KADIM++GGR ++A+K ++P++DINQER +YAGY G++ LA+++ S+ NPIW Sbjct: 396 KADIMLSGGRTQFIALKAKMPWLDINQERQHSYAGYHGVVELARQIDLSIHNPIWAQVRE 455 Query: 456 PAPWD 460 APW+ Sbjct: 456 AAPWE 460 >MTH1565 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 268 Score = 311 bits (796), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 0/406 (0%), Positives = 0/406 (0%), Gaps = 6/406 (1%) Query: 50 CSFDGAQVTLLPIMDAAHLVHGPIACAGNSWESRGTLSSGPSLSQYGFATDLTDSDIIFG 109 + + G + G +G + + G Sbjct: 64 MDTPQYLRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKGTLVDIG 123 Query: 110 GEKKLKDSIDYIAGRFAPPAIFVYSTCVTALTGEDMDAVCKTAAERLGIPVIPVNSPGFV 169 +K R + + E D + Sbjct: 124 ADKLALCREKLTVNRIMSFRVVRLGKEILIEPDEPEDRYWGYEVLDTRRNLAESLKTVGA 183 Query: 170 G-SKNLGNRLAGDALLQYVIGTGEPSPDMPMGINLIGEYNIAGEMWDIEKLMNSAGISVT 228 + + + T I G Y E+ + G Sbjct: 184 DVVVATSRNASPITSILDEVKTRMRGAR-EAAILFGGPYKGLPEIDADIWVNTLPGQCTE 242 Query: 229 SRITGDARYKEVTWAHRAKVNMVVCSRALLGLAREMESRYGIPYFEGSFYGAKETTYSLR 288 Sbjct: 243 302 Query: 289 QMAYLMNDRDMERRVDRLTEREEARLSQELRPYRKILKGKKAVLYTGGVKSWSVISALKE 348 Sbjct: 303 ---- 358 Query: 349 LGVQVVGVGTNKSTEDDVRRIADRVGDDTEYIPEGGAGRILKTVRERKADIMIAGGRNMY 408 Sbjct: 359 418 Query: 409 VAMKEQIPFIDINQERHKAYAGYEGLLRLAKELTYSLANPIWKLAA 454 Sbjct: 419 464 >CAC0258 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 466 Score = 303 bits (775), Expect = 5e-82, Method: Compositional matrix adjust. Identities = 155/405 (38%), Positives = 248/405 (61%), Gaps = 6/405 (1%) Query: 50 CSFDGAQVTLLPIMDAAHLVHGPIACAGNSWESRGTLSSGPSLSQYGFATDLTDSDIIFG 109 C + GA+V L PI DA HL+HGPI CA +W+ RG+LSS L + F+TDL ++D+IFG Sbjct: 63 CVYCGARVVLNPITDAFHLIHGPIGCASYTWDLRGSLSSESELFRNSFSTDLRETDVIFG 122 Query: 110 GEKKLKDSIDYIAGRFAPPAIFVYSTCVTALTGEDMDAVCKTAAERL-GIPVIPVNSPGF 168 GEKKL+ +ID I +F P IF+Y+TC+ + G+D+DAVC+ A + + VI V SPGF Sbjct: 123 GEKKLRAAIDEIFIKFHPKLIFIYATCIVGVIGDDIDAVCREAEIKYPMMRVIAVKSPGF 182 Query: 169 VGSKNLGNRLAGDALLQYVIGTGEPSPDMPM-GINLIGEYNIAGEMWDIEKLMNSAGISV 227 G+K +G R A +A+L+ + G+ D + G+N +G++N+AGE W I+ + G+ V Sbjct: 183 SGNKAMGYRAACNAILKLI---GDRKSDKKVKGVNYLGDFNLAGEAWVIQSYLKKIGVPV 239 Query: 228 TSRITGDARYKEVTWAHRAKVNMVVCSRALLGLAREMESRYGIPYFEGSFYGAKETTYSL 287 ++ITGD +Y+E+ A A +N+V C+ ++ LA++M+ YGIPY SF G ++T SL Sbjct: 240 VAKITGDGKYEELLKAPEASLNIVQCAGSMEYLAKKMQELYGIPYIRISFLGVEDTENSL 299 Query: 288 RQMAYLMNDRDMERRVDRLTEREEARLSQELRPYRKILKGKKAVLYT-GGVKSWSVISAL 346 Q+A+++ D+ + ++ + EE ++ ++ YRK L+GKKA +Y GG K+ S+I Sbjct: 300 LQIAHILGGEDIVEKTEKFIQEEEDKVKDKIDFYRKRLEGKKAAIYVGGGYKAISLIKQF 359 Query: 347 KELGVQVVGVGTNKSTEDDVRRIADRVGDDTEYIPEGGAGRILKTVRERKADIMIAGGRN 406 K LG++ V VGT D I + T + + + + + E+ ADI++ G + Sbjct: 360 KSLGIETVMVGTQTGKPRDYEIIKEITNRGTVILDDANPSELERFMLEKGADILVGGVKE 419 Query: 407 MYVAMKEQIPFIDINQERHKAYAGYEGLLRLAKELTYSLANPIWK 451 +A K + F D N ER +G+ G L A+E+ ++ +P+WK Sbjct: 420 RPLAYKLGVAFCDHNHERKHILSGFIGALNFAEEIDLTVNSPVWK 464 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,373,820 Number of extensions: 1673201 Number of successful extensions: 6224 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 6170 Number of HSP's successfully gapped: 20 Length of query: 468 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 358 Effective length of database: 25,691,691 Effective search space: 9197625378 Effective search space used: 9197625378 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)