BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0151.0005.p (488 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr5906 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 663 0.0 SMa0827 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 658 0.0 all1454 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 647 0.0 CAC0256 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 376 e-104 MTH1563 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 326 4e-89 >mlr5906 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 501 Score = 663 bits (1711), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/495 (64%), Positives = 382/495 (77%), Gaps = 14/495 (2%) Query: 1 MGLNIEEN----KKIVEEVLEAYPKKAKKDREKHFQINTEEAQSCGT------CALKSNI 50 MGL+ E + K++E+VL YP KA K R+KH + T + ++ G C +KSNI Sbjct: 1 MGLDYENDGALHAKLIEDVLSQYPDKAAKRRKKHLSVATSKDEAGGEDKGLSECDVKSNI 60 Query: 51 KSRPGVMTPRGCSFAGSKGVVWGPIKDMVHISHGPIGWGQXXXXSWGTRRNYANGTLGID 110 KS PGVMT RGC++AGSKGVVWGP+KDMVHISHGP+G GQ SW RRNY GT GID Sbjct: 61 KSIPGVMTIRGCAYAGSKGVVWGPVKDMVHISHGPVGCGQY---SWSQRRNYYVGTTGID 117 Query: 111 NFTAMQITSDFQETDIVFGGDKKLEVIIREITEMFPLAKGISVQSECPVGLIGDDIEAVS 170 F MQ TSDFQE DIVFGGDKKLE II EI ++FPL+ GISVQSECP+GLIGDDIEAVS Sbjct: 118 TFVTMQFTSDFQEKDIVFGGDKKLEKIIDEIEDLFPLSGGISVQSECPIGLIGDDIEAVS 177 Query: 171 KKMAKELDMPIVPVRCEGFRGVSQSLGHHIANDAIRDFVLGKAELA-ETGPYDVNIIGDY 229 +K AKE + IVPVRCEGFRGVSQSLGHHIANDAIRD+V K ++A E+GPYDVN++GDY Sbjct: 178 RKKAKEHEKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKDDVAFESGPYDVNVVGDY 237 Query: 230 NIGGDAWASRILLEEMGLRVIAQWSGDGTLNELEIAHKAKLNLIHCHRSMNYMVEHMEKA 289 NIGGDAWASRILLEE+GLRV+ WSGD TL E+E A KAKLNLIHC+RSMNY+ HME+ Sbjct: 238 NIGGDAWASRILLEEIGLRVVGNWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEK 297 Query: 290 YDIPWMEYNFFGPSKTYESLRAIAARFDATIQQNCEKMIAKHKPAMDAIINKYKPRLENK 349 Y I WMEYNFFGPS+ SLR IA F I++ EK+IAK++P +DA+I KY+PRLE + Sbjct: 298 YGIAWMEYNFFGPSQIEASLRNIAKHFGPEIEEKTEKVIAKYRPLVDAVIAKYQPRLEGR 357 Query: 350 KVLLMIGGLRSRHTIGAYEDLGMDIVASGYEFAHKDDYEKTFPMMKEGTIIMDDPTAYEL 409 V+L +GGLR RH I AYEDL M IV +GYEFAH DDY++T +K GT++ DD T YEL Sbjct: 358 TVMLYVGGLRPRHVITAYEDLAMVIVGTGYEFAHNDDYQRTGHYVKNGTLLYDDVTGYEL 417 Query: 410 EELAQKLNVDLVGSGVKEKYVYHKMGVPFRQMHSWDYSGPYHGFDGFKIFAKDMDMTVNS 469 E+ + + DLVGSG+KEKY KMG+PFRQMHSWDYSGPYHG+DGF IFA+DMD+ +N+ Sbjct: 418 EKFIEGIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAINN 477 Query: 470 PVWNLIRKKEKAQKE 484 PVW L R K K+ Sbjct: 478 PVWGLYRAPWKKMKD 492 >SMa0827 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 500 Score = 658 bits (1698), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/485 (65%), Positives = 372/485 (76%), Gaps = 14/485 (2%) Query: 1 MGLNIEEN----KKIVEEVLEAYPKKAKKDREKHFQI------NTEEAQSCGTCALKSNI 50 M L+ E + +K++EEVL YP KA K R+KH + EE Q C +KSNI Sbjct: 1 MSLDYENDNALHEKLIEEVLSHYPDKAAKRRKKHLSVAKNKQETAEEGQVVSECDVKSNI 60 Query: 51 KSRPGVMTPRGCSFAGSKGVVWGPIKDMVHISHGPIGWGQXXXXSWGTRRNYANGTLGID 110 KS PGVMT RGC++AGSKGVVWGPIKDMVHISHGP+G GQ SW RRNY GT GID Sbjct: 61 KSIPGVMTIRGCAYAGSKGVVWGPIKDMVHISHGPVGCGQY---SWSQRRNYYVGTTGID 117 Query: 111 NFTAMQITSDFQETDIVFGGDKKLEVIIREITEMFPLAKGISVQSECPVGLIGDDIEAVS 170 F MQ TSDFQE DIVFGGDKKLE II EI E+FPL G++VQSECP+GLIGDDIEAVS Sbjct: 118 AFVTMQFTSDFQEKDIVFGGDKKLEKIIDEIEELFPLNNGVTVQSECPIGLIGDDIEAVS 177 Query: 171 KKMAKELDMPIVPVRCEGFRGVSQSLGHHIANDAIRDFVLGKAELA-ETGPYDVNIIGDY 229 +K A+E IVPVRCEGFRGVSQSLGHHIANDAIRD+V E+A E G YDVN+IGDY Sbjct: 178 RKKAEEYKTTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDTTEVAYEAGRYDVNVIGDY 237 Query: 230 NIGGDAWASRILLEEMGLRVIAQWSGDGTLNELEIAHKAKLNLIHCHRSMNYMVEHMEKA 289 NIGGDAWASRILLEE+GL V+ WSGD TL E+E A AKLNLIHC+RSMNY+ HME+ Sbjct: 238 NIGGDAWASRILLEEIGLHVVGNWSGDATLAEIERAPTAKLNLIHCYRSMNYICRHMEEK 297 Query: 290 YDIPWMEYNFFGPSKTYESLRAIAARFDATIQQNCEKMIAKHKPAMDAIINKYKPRLENK 349 Y +PWMEYNFFGPS+ SLR IA F I++ E++IAK+ DA+I+KY PRL K Sbjct: 298 YGVPWMEYNFFGPSQIEASLRQIAKHFGPEIEERAERVIAKYSGLTDAVIDKYWPRLHGK 357 Query: 350 KVLLMIGGLRSRHTIGAYEDLGMDIVASGYEFAHKDDYEKTFPMMKEGTIIMDDPTAYEL 409 +V+L +GGLR RH I AYEDLGM+IV +GYEFAH DDY++T +KEGT+I DD T YEL Sbjct: 358 RVMLYVGGLRPRHVITAYEDLGMEIVGTGYEFAHNDDYQRTGHYVKEGTLIYDDVTGYEL 417 Query: 410 EELAQKLNVDLVGSGVKEKYVYHKMGVPFRQMHSWDYSGPYHGFDGFKIFAKDMDMTVNS 469 E+ +++ DLVGSG+KEKY KMG+PFRQMHSWDYSGPYHG+DGF IFA+DMD+ VN+ Sbjct: 418 EKFIERIRPDLVGSGIKEKYSVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAVNN 477 Query: 470 PVWNL 474 PVW+L Sbjct: 478 PVWDL 482 >all1454 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 497 Score = 647 bits (1669), Expect = 0.0, Method: Compositional matrix adjust. Identities = 299/479 (62%), Positives = 375/479 (78%), Gaps = 14/479 (2%) Query: 5 IEENKKIVEEVLEAYPKKAKKDREKHFQINTEEAQSCGTCALKSNIKSRPGVMTPRGCSF 64 ++ENK++++EVL+AYP+K++K REKH ++ E CG +KSNIKS PGVMT RGC++ Sbjct: 10 VDENKELIQEVLKAYPEKSRKKREKHLNVHEENKSDCG---VKSNIKSVPGVMTARGCAY 66 Query: 65 AGSKGVVWGPIKDMVHISHGPIGWGQXXXXSWGTRRNYANGTLGIDNFTAMQITSDFQET 124 AGSKGVVWGPIKDM+HISHGP+G G SW RRNY G GI++F M TSDFQE Sbjct: 67 AGSKGVVWGPIKDMIHISHGPVGCG---YWSWSGRRNYYVGVTGINSFGTMHFTSDFQER 123 Query: 125 DIVFGGDKKLEVIIREITEMFPLAKGISVQSECPVGLIGDDIEAVSKKMAKELDMPIVPV 184 DIVFGGDKKL +I E+ +FPL +G+S+QSECP+GLIGDDIEAV+KK +K++ P+VP+ Sbjct: 124 DIVFGGDKKLTKLIEELDVLFPLNRGVSIQSECPIGLIGDDIEAVAKKTSKQIGKPVVPL 183 Query: 185 RCEGFRGVSQSLGHHIANDAIRDFVLGKAELA--------ETGPYDVNIIGDYNIGGDAW 236 RCEGFRGVSQSLGHHIANDAIRD++ + + E PYDV +IGDYNIGGDAW Sbjct: 184 RCEGFRGVSQSLGHHIANDAIRDWIFPEYDKLKKENRLDFEPSPYDVALIGDYNIGGDAW 243 Query: 237 ASRILLEEMGLRVIAQWSGDGTLNELEIAHKAKLNLIHCHRSMNYMVEHMEKAYDIPWME 296 ASR+LLEEMGLRV+AQWSGDGTLNEL AKL LIHC+RSMNY+ +E+ Y +PWME Sbjct: 244 ASRMLLEEMGLRVVAQWSGDGTLNELIQGPAAKLVLIHCYRSMNYICRSLEEQYGMPWME 303 Query: 297 YNFFGPSKTYESLRAIAARFDATIQQNCEKMIAKHKPAMDAIINKYKPRLENKKVLLMIG 356 +NFFGP+K SLR IAA+FD+ IQ+N EK+IAK+ P M+A+++KY+PRLE V+L +G Sbjct: 304 FNFFGPTKIAASLREIAAKFDSKIQENAEKVIAKYTPVMNAVLDKYRPRLEGNTVMLYVG 363 Query: 357 GLRSRHTIGAYEDLGMDIVASGYEFAHKDDYEKTFPMMKEGTIIMDDPTAYELEELAQKL 416 GLR RH + A+EDLG+ +V +GYEFAH DDY++T + TII DD TAYE EE + Sbjct: 364 GLRPRHVVPAFEDLGIKVVGTGYEFAHNDDYKRTTHYIDNATIIYDDVTAYEFEEFVKAK 423 Query: 417 NVDLVGSGVKEKYVYHKMGVPFRQMHSWDYSGPYHGFDGFKIFAKDMDMTVNSPVWNLI 475 DL+ SG+KEKYV+ KMG+PFRQMHSWDYSGPYHG+DGF IFA+DMD+ +NSP W+LI Sbjct: 424 KPDLIASGIKEKYVFQKMGLPFRQMHSWDYSGPYHGYDGFAIFARDMDLALNSPTWSLI 482 >CAC0256 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 531 Score = 376 bits (965), Expect = e-104, Method: Compositional matrix adjust. Identities = 201/517 (38%), Positives = 293/517 (56%), Gaps = 60/517 (11%) Query: 9 KKIVEEVLEAYPKKAKKDREKHFQINTEEAQSCGTCALKSNIKSRPGVMTPRGCSFAGSK 68 K I E++ E YP K K+R++H I T++ + +++N+++ PG++T RGC +AG K Sbjct: 2 KNIPEKIFEKYPAKTFKNRKQHMVIKTKDNLNP---VIQANVRTIPGIITNRGCCYAGCK 58 Query: 69 GVVWGPIKDMVHISHGPIGWGQXXXXSWGTRRNYANGTLGIDNFTAMQITSDFQETDIVF 128 GVV GPIKDM+HI+HGPIG +WG RRN A G NF ++D QE DIVF Sbjct: 59 GVVMGPIKDMIHITHGPIGCS---YYTWGVRRNKAKAEDGGQNFIEYVFSTDMQERDIVF 115 Query: 129 GGDKKLEVIIREITEMFPLAKGISVQSECPVGLIGDDIEAVSKKMAKELDMPIVPVRCEG 188 GG+ KL I+E E+F K I + + CPVGLIGDDI AV+++ ++ + ++ CEG Sbjct: 116 GGEGKLRKAIKEAVEIFH-PKAIGIYATCPVGLIGDDINAVARESTEKYGIQVIAFSCEG 174 Query: 189 FRGVSQSLGHHIANDAIRDFVLGKAELAETGPYDVNIIGDYNIGGDAWASRILLEEMGLR 248 ++GVSQS GHHIAN+ I D V+G + Y VNI+G+YNIGGDAW +L+++G Sbjct: 175 YKGVSQSAGHHIANNNILDTVIGTGDWKHK-KYSVNILGEYNIGGDAWEIERILKKIGYT 233 Query: 249 VIAQWSGDGTLNELEIAHKAKLNLIHCHRSMNYMVEHMEKAYDIPWMEYNFFGPSKTYES 308 V+ + SGDG+ ++ A A LNL+ CHRS+NY+ E M Y IPW++ NF G + T ++ Sbjct: 234 VVGRLSGDGSYERVKNADTADLNLVQCHRSINYIAEMMYTKYGIPWIKTNFIGVTSTIQT 293 Query: 309 LRAIAARF-DATIQQNCEKMIAKHKPAMDAIINKYKPRLENKKVLLMIGGLRSRHTIGAY 367 LR +A F D I + E++I++ ++ + +K RLE K L +GG R+ H Sbjct: 294 LRDMAKVFDDPYIYKKTEEVISEELSNIEGEMAYFKERLEGKTACLFVGGSRAHHYQMLL 353 Query: 368 EDLGMDIVASGYEFAHKDDYE--------------------------------------- 388 +D G+ V +GYEF H+DDYE Sbjct: 354 KDYGVKTVLAGYEFGHRDDYEGREVIPTIKADADSKNIPELHVEKDPKKYRIIIPEDRYE 413 Query: 389 ------------KTFPMMKEGTIIMDDPTAYELEELAQKLNVDLVGSGVKEKYVYHKMGV 436 F M+EG + +DD +E E+ + L D+ SGVK+KYV KMG+ Sbjct: 414 VLKQEIPLEYYGGMFKDMEEGAVAVDDLNHHETEQFIKLLKPDMFFSGVKDKYVIQKMGI 473 Query: 437 PFRQMHSWDYSGPYHGFDGFKIFAKDMDMTVNSPVWN 473 +Q+HS+DY+GPY GF G +F K++ +P WN Sbjct: 474 LSKQLHSYDYTGPYAGFKGAVVFGKELVAGAFTPAWN 510 >MTH1563 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 268 Score = 326 bits (836), Expect = 4e-89, Method: Compositional matrix adjust. Identities = 0/460 (0%), Positives = 0/460 (0%), Gaps = 20/460 (4%) Query: 26 DREKHFQINTEE--AQSCGTCALKSNIKSRPGVMTPRGCSFAGSKGVVWGPIKDMVHISH 83 + + + + G + + M + Sbjct: 14 LTAETGDLKIKTYKVVLIARA----ASIFGVKRIVIYHDDADGEARFIRDILTYMDTPQY 69 Query: 84 GPIGWGQXXXXSWGTRR-----NYANGTL-GIDNFTAMQIT-SDFQETDIVFGGDKKLEV 136 + T + D + Sbjct: 70 LRRKV---FPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKGTLVDIGADK 126 Query: 137 IIREITEMFPLAKGISVQSECPVGLIGDDIEAVSKKMAKELDMPIVPVRCEGFRGVSQSL 196 + ++ ++ + E + E+ + + + Sbjct: 127 LALCREKLTVNRIMSFRVVRLGKEILIEPDEPEDRYWGYEVLDTRRNLAESLKTVGADVV 186 Query: 197 GHHIANDAIRDFVLGKAELAETGPYDVNIIGDYNIGGDAWASRILLEEMGLRVIAQWSGD 256 N + +L + + G + I+ G + + Sbjct: 187 VATSRNASPITSILDEVKTRMRGAREAAILFGGPYKGLPEIDADIWVNTLPGQCTETVRT 246 Query: 257 GTLNELEIAHKAKLNLIHCHRSMNYMVEHMEKAYDIPWMEYNFFGPSKTYESLRAIAARF 316 Sbjct: 247 306 Query: 317 DATIQQNCEKMIAKHKPAMDAIINKYKPRLENKKVLLMIGGLRSRHTIGAYED-LGMDIV 375 Sbjct: 307 -- 364 Query: 376 ASGYEFAHKDDYEKTFPMMKEGTIIMDDPTAYELEELAQKLNVDLVGSGVKEKYVYHKMG 435 Sbjct: 365 424 Query: 436 VPFRQMHSWDYSGPYHGFDGFKIFAKDMDMTVNSPVWNLI 475 Sbjct: 425 - 463 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,097,140 Number of extensions: 1646032 Number of successful extensions: 3481 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 3428 Number of HSP's successfully gapped: 18 Length of query: 488 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 378 Effective length of database: 25,691,691 Effective search space: 9711459198 Effective search space used: 9711459198 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)