BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0149.0038.p (315 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF0813 [HE] COG0111 Phosphoglycerate dehydrogenase and related d... 158 1e-38 MK0297 [HE] COG0111 Phosphoglycerate dehydrogenase and related d... 143 4e-34 MA1334 [HE] COG0111 Phosphoglycerate dehydrogenase and related d... 133 3e-31 sll1908 [HE] COG0111 Phosphoglycerate dehydrogenase and related ... 128 1e-29 BMEI0349 [HE] COG0111 Phosphoglycerate dehydrogenase and related... 127 3e-29 >AF0813 [HE] COG0111 Phosphoglycerate dehydrogenase and related dehydrogenases Length = 527 Score = 158 bits (399), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 91/250 (36%), Positives = 136/250 (54%), Gaps = 7/250 (2%) Query: 34 QEMLHHIEDCQ-VIIPEHIKVDHSLLSIAKKLKLV-QTGAGFDNVDVPACTQLGIWXXXX 91 +E++ + + +++ KVD ++ AK LK++ + G G DN+D+ A TQ GI Sbjct: 33 EELIREVPKYEAIVVRSQTKVDAEVIQAAKNLKIIGRAGVGVDNIDINAATQRGIVVVNA 92 Query: 92 XXXXXXXXXEHVMALILSYYKNIPFLDTFMKNKMDESQLDYTGSELXXXXXXXXXXXXXX 151 EH +AL+L+ + IP D +K E + + G EL Sbjct: 93 PGGNTISTAEHAIALMLAAARKIPQADRSVKEGKWERK-KFMGIELRGKTAGVIGLGRVG 151 Query: 152 XXVAAFCRVFDMNVLAY---ARNAVVQSDGFVKMTDFDTLLSTSDIISVHIPLNQQTKQL 208 VA C+ +MNVLAY + G VK+ DFDTLL++SD+I+VH+P ++T L Sbjct: 152 FEVAKRCKALEMNVLAYDPFVSKERAEQIG-VKLVDFDTLLASSDVITVHVPRTKETIGL 210 Query: 209 INKAVFKKMKNSTLFINTARGGIVNERDLIDALKNGDISGACLDVFESEPLPIDSELRNL 268 I K F+KMK+ + +N ARGGIV+E L +A+K G ++ A LDV+E EP D+ L L Sbjct: 211 IGKGQFEKMKDGVIVVNAARGGIVDEAALYEAIKAGKVAAAALDVYEKEPPSPDNPLLKL 270 Query: 269 GNVILTPHTA 278 NV+ TPH A Sbjct: 271 DNVVTTPHIA 280 >MK0297 [HE] COG0111 Phosphoglycerate dehydrogenase and related dehydrogenases Length = 522 Score = 143 bits (360), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 93/285 (32%), Positives = 140/285 (49%), Gaps = 10/285 (3%) Query: 34 QEMLHHIEDCQV-IIPEHIKVDHSLLSIAKKLKLV-QTGAGFDNVDVPACTQLGIWXXXX 91 +E+ H+ D ++ +V L+ AK LK++ + G G DN+DV A T+ GI Sbjct: 33 EEIREHVRDADAWVVRSGTRVTRELIEEAKNLKVIARAGVGVDNIDVKAATERGIIVVNA 92 Query: 92 XXXXXXXXXEHVMALILSYYKNIPFLD-TFMKNKMDESQLDYTGSELXXXXXXXXXXXXX 150 EH M LIL+ + IP D + + + D + + G EL Sbjct: 93 PESSSISVAEHTMGLILALARKIPQADRSVRRGEWDRKR--FMGVELAGKTLGLIGLGRI 150 Query: 151 XXXVAAFCRVFDMNVLAY---ARNAVVQSDGFVKMTDFDTLLSTSDIISVHIPLNQQTKQ 207 VA + F+M V AY V + G + + + LL +D++S+H+PL ++T+ Sbjct: 151 GQQVAKRAKAFEMEVTAYDPYIPEKVAEELGVELVDELEELLERADVVSIHVPLTEETEG 210 Query: 208 LINKAVFKKMKNSTLFINTARGGIVNERDLIDALKNGDISGACLDVFESEPLPIDSELRN 267 +I + K+MK+S +N ARG IV+E LI ALK G I+GA LDVF EP D L Sbjct: 211 MIGEEELKRMKSSAFLVNCARGKIVDEEALIKALKEGWIAGAALDVFAEEPPGEDHPLYE 270 Query: 268 LGNVILTPHTAGMPDGRKFHKKRYDFFINNIKRIENGEEPESKLN 312 L NV+LTPH G + + I+R+ GE PE+ +N Sbjct: 271 LDNVVLTPHIGGSTG--EAQRAAGLIVAREIERVLKGEIPENVVN 313 >MA1334 [HE] COG0111 Phosphoglycerate dehydrogenase and related dehydrogenases Length = 319 Score = 133 bits (335), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 85/248 (34%), Positives = 125/248 (50%), Gaps = 6/248 (2%) Query: 35 EMLHHIEDCQVIIPEHIKVDHSLLSIAKKLKLVQT-GAGFDNVDVPACTQLGIWXXXXXX 93 E + I+D +++ V L A +LK++ GFDNVD+ T G+ Sbjct: 37 EFIDRIKDADIVVVGRYGVSAEALRSAPRLKMISLWQTGFDNVDLEEATDHGVIVSNVPS 96 Query: 94 XXXXXXXEHVMALILSYYKNIPFLDTFMKNKMDESQLDYTGSELXXXXXXXXXXXXXXXX 153 E V AL L+ + + D ++ + + + Y G++L Sbjct: 97 YAFESVAEFVFALTLNLLRRVHLADMNLREGLFDWKY-YVGNQLMSKTIGVLGTGEIGKR 155 Query: 154 VAAFCRVFDMNVL---AYARNAVVQSDGFVKMTDFDTLLSTSDIISVHIPLNQQTKQLIN 210 V F+MNVL A+ ++ G VK D DTLLS SDI+++H+PL +T+ +I Sbjct: 156 VIQIAHGFNMNVLSVTAHPSPERAKALG-VKFVDLDTLLSESDIVTLHVPLTPETEHMIG 214 Query: 211 KAVFKKMKNSTLFINTARGGIVNERDLIDALKNGDISGACLDVFESEPLPIDSELRNLGN 270 KMK + + INTARG +V E L++ALK I+GA LDVFE EPL +DS L + N Sbjct: 215 ARELAKMKPTAILINTARGKVVEEAALMEALKEKKIAGAGLDVFEREPLSMDSPLLEMHN 274 Query: 271 VILTPHTA 278 V+LTPH A Sbjct: 275 VVLTPHIA 282 >sll1908 [HE] COG0111 Phosphoglycerate dehydrogenase and related dehydrogenases Length = 554 Score = 128 bits (321), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 80/250 (32%), Positives = 119/250 (47%), Gaps = 29/250 (11%) Query: 46 IIPEH--------IKVDHSLLSIAKKLKLV-QTGAGFDNVDVPACTQLGIWXXXXXXXXX 96 I+PE+ KV ++ +LK++ + G G DN+DVPA T+ GI Sbjct: 66 IVPEYDAIMLRSATKVTEKIIQAGSQLKIIGRAGVGVDNIDVPAATRQGIVVVNSPEGNT 125 Query: 97 XXXXEHVMALILSYYKNIPFLDTFMKNKMDESQLDYTGSELXXXXXXXXXXXXXXXXVAA 156 EH +A++++ ++IP + +K E + + G+E+ VA Sbjct: 126 IAAAEHALAMMMALARHIPDANKSVKESKWERK-QFIGTEVYKKTLGVVGLGKIGSHVAG 184 Query: 157 FCRVFDMNVLAYARNAVVQSDGFVK----------MTDFDTLLSTSDIISVHIPLNQQTK 206 + M +LAY D F+ + D D L S +D I++HIP +T Sbjct: 185 VAKAMGMKLLAY--------DPFISQERADQIGCTLVDLDLLFSEADFITLHIPKTPETA 236 Query: 207 QLINKAVFKKMKNSTLFINTARGGIVNERDLIDALKNGDISGACLDVFESEPLPIDSELR 266 LIN KMK + IN +RGGI++E L+ A++ I GA LDVF EPL +S LR Sbjct: 237 NLINAETLAKMKPTARIINCSRGGIIDEEALVTAIETAQIGGAALDVFAQEPLG-ESRLR 295 Query: 267 NLGNVILTPH 276 NVILTPH Sbjct: 296 EFSNVILTPH 305 >BMEI0349 [HE] COG0111 Phosphoglycerate dehydrogenase and related dehydrogenases Length = 538 Score = 127 bits (318), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 80/229 (34%), Positives = 122/229 (53%), Gaps = 7/229 (3%) Query: 52 KVDHSLLSIAKKLKLV-QTGAGFDNVDVPACTQLGIWXXXXXXXXXXXXXEHVMALILSY 110 KV L++ AKKLK+V + G G DNVD+PA ++ GI EH +AL+ + Sbjct: 61 KVTEKLIAAAKKLKVVGRAGIGVDNVDIPAASRRGIIVMNTPFGNSITTAEHAIALMFAV 120 Query: 111 YKNIPFLDTFMKNKMDESQLDYTGSELXXXXXXXXXXXXXXXXVAAFCRVFDMNVLAY-- 168 + +P DT + E + G E+ VA M+V+A+ Sbjct: 121 ARQLPEADTSTRAGKWEKN-RFMGVEITGKTLGVVGCGNIGSIVATRGIGLKMHVVAFDP 179 Query: 169 -ARNAVVQSDGFVKMTDFDTLLSTSDIISVHIPLNQQTKQLINKAVFKKMKNSTLFINTA 227 +A Q G V+ + D LL+ +D I++H PL +T+ +IN KMK +N A Sbjct: 180 FLSDARAQELG-VEKVELDELLARADFITLHTPLIDKTRNIINAQTLAKMKPGVRIVNCA 238 Query: 228 RGGIVNERDLIDALKNGDISGACLDVFESEPLPIDSELRNLGNVILTPH 276 RGG++ E+DLI ALK+G ++GA +DV+E+EP ++EL +L NV+ TPH Sbjct: 239 RGGLIVEKDLIAALKSGHVAGAGIDVYETEP-ATENELFSLPNVVCTPH 286 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,417,882 Number of extensions: 780555 Number of successful extensions: 2787 Number of sequences better than 1.0e-05: 160 Number of HSP's gapped: 2687 Number of HSP's successfully gapped: 160 Length of query: 315 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 209 Effective length of database: 26,153,027 Effective search space: 5465982643 Effective search space used: 5465982643 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)