BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0149.0028.p (788 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pli0050 [T] COG2205 Osmosensitive K+ channel histidine kinase 241 3e-63 lin2827 [T] COG2205 Osmosensitive K+ channel histidine kinase 228 2e-59 SA0067 [T] COG2205 Osmosensitive K+ channel histidine kinase 218 3e-56 SA1882 [T] COG2205 Osmosensitive K+ channel histidine kinase 201 6e-51 >pli0050 [T] COG2205 Osmosensitive K+ channel histidine kinase Length = 888 Score = 241 bits (616), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 146/367 (39%), Positives = 211/367 (57%), Gaps = 19/367 (5%) Query: 414 RRGRFKVFIGAAPGVGKTYTMLREGNHLLKKGVDVVIGLLETHGRQETGQQVAELPFIER 473 RRG+ +++ G A GVGKT+ ML + L+K GVD+V+G +E H R ET + + L I Sbjct: 15 RRGKLRIYFGYAAGVGKTFAMLNDAQELVKTGVDLVVGYVEPHARPETMKLLNGLEKIPT 74 Query: 474 AKIKYSETVLEEMDTGAILRRNPEVVLVDELAHTNVPGSKYKKRYEDVMEILNAGISVIS 533 I Y L E D + L+RNP++++VDELAHTN ++KKRY+D+ E+L AGI V + Sbjct: 75 KPILYKGVKLYEFDLDSALKRNPKLIIVDELAHTNAYACRHKKRYQDIEELLQAGIDVFT 134 Query: 534 TVNVQHLESLNDAVAQITGVRVRETVPDSILRLADEVELIDVAPQALQARMKDGKIYARE 593 TVNVQH+ESLND V IT + V E +PD++ ADE+EL+D+ PQ L R+ GKIY + Sbjct: 135 TVNVQHIESLNDIVESITNIHVLERIPDAVFDGADEIELVDIEPQDLLDRLNKGKIYREK 194 Query: 594 KIDQALDNFFKIGNLIALRELSLREIADDVDERLEAWERVSSLRGPWNK--QEVIFVCVK 651 + QA+ NFF LIALRE++LR AD V E S++ P N +E I VC+ Sbjct: 195 QARQAVLNFFTTDKLIALREIALRRTADQVKRIAE-----SNIVNPKNNYGKEHILVCIS 249 Query: 652 LSQSAGKLIRRGFRTAFRLKADWIVAYVHSAKELTEDELKMKASI-ENLTL--RLGGKFE 708 SQ K+IR R A LK +V + E E +K + S+ +NL L +LG + Sbjct: 250 PSQGNEKVIRTAARMAEALKGKLTAVFVETP-EYEESSIKEQESLRKNLRLAEQLGAQIS 308 Query: 709 MISSSGPSSRLAEVIQAKADEHHATQLIIGQCDRPAWKRLW-EGTIIKKLLRTARHMDIL 767 + S +LAE A+ AT++I+G R + ++ W + ++ KL DI Sbjct: 309 SVYGEDISQQLAEY----ANVSQATKIILG---RSSKRKSWLKPKLVDKLSEQIDSTDIY 361 Query: 768 IVANYDP 774 I+ + P Sbjct: 362 IIPDTQP 368 Score = 164 bits (416), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 108/361 (29%), Positives = 190/361 (52%), Gaps = 20/361 (5%) Query: 24 RGRLKIFIGAVSGSGKTYHMLREGTALQQQGIDVVICAVSTLQRPETIEQIGQLERIPSI 83 RG+L+I+ G +G GKT+ ML + L + G+D+V+ V RPET++ + LE+IP+ Sbjct: 16 RGKLRIYFGYAAGVGKTFAMLNDAQELVKTGVDLVVGYVEPHARPETMKLLNGLEKIPTK 75 Query: 84 HWVHDGTEKKDLNVEALIQRNPEVVLVDGLAHRNRPDAVHKTRLEDIHFLLGHGISVITT 143 ++ G + + ++++ ++RNP++++VD LAH N HK R +DI LL GI V TT Sbjct: 76 PILYKGVKLYEFDLDSALKRNPKLIIVDELAHTNAYACRHKKRYQDIEELLQAGIDVFTT 135 Query: 144 VNVYELEGVRELAQKYMGIEVEETVSAHTLELADEIRLIDVTPETLLQRVSEGHVRNSKT 203 VNV +E + ++ + I V E + + ADEI L+D+ P+ LL R+++G + K Sbjct: 136 VNVQHIESLNDIVESITNIHVLERIPDAVFDGADEIELVDIEPQDLLDRLNKGKIYREKQ 195 Query: 204 A-----ALFQRGTLGVLRELALRLVAEGVNDSLEKHREQMGMLGPSG--AAERILVSTQY 256 A F L LRE+ALR A+ V K + ++ P E ILV Sbjct: 196 ARQAVLNFFTTDKLIALREIALRRTADQV-----KRIAESNIVNPKNNYGKEHILVCISP 250 Query: 257 HWNGSIYVRRGQQVAKRLNGELIIITFTNP--KRPLTKEQAAFKESLVKLVRKVGAAFEE 314 +R ++A+ L G+L + P + KEQ + +++L +L ++GA Sbjct: 251 SQGNEKVIRTAARMAEALKGKLTAVFVETPEYEESSIKEQESLRKNL-RLAEQLGAQISS 309 Query: 315 LPFHSRRRLPERLIQYALEHNVTRIMLGHSRQSRWQEFWQGSVVNDIIRKSRNMDIFMVA 374 + + ++L +YA T+I+LG R S+ + + + +V+ + + + DI+++ Sbjct: 310 V---YGEDISQQLAEYANVSQATKIILG--RSSKRKSWLKPKLVDKLSEQIDSTDIYIIP 364 Query: 375 D 375 D Sbjct: 365 D 365 >lin2827 [T] COG2205 Osmosensitive K+ channel histidine kinase Length = 182 Score = 228 bits (582), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 0/371 (0%), Positives = 0/371 (0%), Gaps = 21/371 (5%) Query: 416 GRFKVFIGAAPGVGKTYTMLREGNHLLKKGVDVVIGLLETHGRQETGQQVAELPFIERAK 475 + + + + T+ Q + Sbjct: 24 AHTADTRLHLAFSSWLFNAKGQLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRR 83 Query: 476 IKYSETVLEEMDTGAILRRNPEVVLVDELAHTNVPGSKYKKRYEDVMEILNAGISVISTV 535 +Y V + V + + + + Sbjct: 84 CRYELGVEITPPESIYPDFRYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCD 143 Query: 536 NVQHLESLNDAVAQITGVRVRETVPDSILRLADEVELIDVAPQALQARMKDGKIYAREKI 595 Sbjct: 144 203 Query: 596 DQALDNFFKIGNLIALRELSLREIADDVDERLEAW-----ERVSSLRGPWNKQEVIFVCV 650 Sbjct: 204 --------- 254 Query: 651 KLSQSAGKLIRRGFRTAFRLKADWIVAYVHSAKE--LTEDELKMKASIENLTLRLGGKFE 708 Sbjct: 255 -- 312 Query: 709 MISSSGPSSRLAEVIQAKADEHHATQLIIGQC-DRPAWKRLWEGTIIKKLLRTARHMDIL 767 Sbjct: 313 -- 370 Query: 768 IVANYDPHIKR 778 Sbjct: 371 381 Score = 142 bits (359), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 0/348 (0%), Positives = 0/348 (0%), Gaps = 18/348 (5%) Query: 9 RKSPEEILLSI---SKLHRGRLKIFIGAVSGSGKTYHMLREGTALQQQGIDVVICAVSTL 65 + + ++ S ++ + ++ V T Sbjct: 5 HVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKGQLLVTRRALSKKAWPGVWTN 64 Query: 66 QRPETIEQIGQLERIPSIHWVHDGTEKKDLNVEALIQRNPEVVLVDGLAHRNRPDAVHKT 125 + E ++ + G+ Sbjct: 65 SVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDFRYRATDPSGIVENEVCPVFAAR 124 Query: 126 RLEDIHFLLGHGISVITTVNVYELEGVRELAQKYMGIEVEETVSAHTLELADEIRLIDVT 185 Sbjct: 125 184 Query: 186 PETLLQRVSEGHVRN---SKTAA--LFQRGTLGVLRELALRLVAEGVNDSLEKHREQMGM 240 Sbjct: 185 ---- 240 Query: 241 LGPSGAAERILVSTQYHWNGSIYVRRGQQVAKRLNGELIIITFTNPKRP-LTK-EQAAFK 298 Sbjct: 241 300 Query: 299 ESLVKLVRKVGAAFEELPFHSRRRLPERLIQYALEHNVTRIMLGHSRQ 346 Sbjct: 301 ---- 344 >SA0067 [T] COG2205 Osmosensitive K+ channel histidine kinase Length = 877 Score = 218 bits (555), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 119/352 (33%), Positives = 201/352 (57%), Gaps = 11/352 (3%) Query: 420 VFIGAAPGVGKTYTMLREGNHLLKKGVDVVIGLLETHGRQETGQQVAELPFIERAKIKYS 479 +++G +PGVGKTY ML G ++G D+ IG +E H R ET V +LP I + K+ Sbjct: 1 MYLGYSPGVGKTYEMLSNGIDQYQEGADIKIGYIEPHQRPETNALVQQLPEISTSSRKFG 60 Query: 480 ETVLEEMDTGAILRRNPEVVLVDELAHTNVPGSKYKKRYEDVMEILNAGISVISTVNVQH 539 +D I+ NP+VVL+DELAHTN+ ++ KRY+D+ EIL+ GI V +T+N+QH Sbjct: 61 SHTFHYIDVSKIIEANPDVVLIDELAHTNISKERHLKRYKDIEEILSHGIDVWTTLNIQH 120 Query: 540 LESLNDAVAQITGVRVRETVPDSILRLADEVELIDVAPQALQARMKDGKIYAREKIDQAL 599 +ESL+ +A +TG++V E VPD + AD E++D++P L R+K GK+Y +E+++ A Sbjct: 121 IESLSGQIALMTGIQVTERVPDQFITSADAFEVVDISPNMLIQRLKAGKVYKKERLETAF 180 Query: 600 DNFFKIGNLIALRELSLREIADDVDERLEAWERVSSLRGPWNKQEVIFVCVKLSQSAGKL 659 NFF NL LREL+LR +AD + ++ + ++ + P I V + S ++ Sbjct: 181 SNFFTYENLTELRELTLRTVADIMSQKGQQYKTKHTDITPH-----IAVAISGSIYNERV 235 Query: 660 IRRGFRTAFRLKADWIVAYVHSAKELTEDELKMKASIENLTL--RLGGKFEMISSSGPSS 717 IR R A++ A + Y+ + +E + +NL L LG + +++ + Sbjct: 236 IREARRAAYKEHAKFTAVYIDVFETPSESRKQDHYVHKNLMLAKALGAEIKVLYAQD--- 292 Query: 718 RLAEVIQAKADEHHATQLIIGQCDRPAWKRLWEGTIIKKLLRTARHMDILIV 769 +A+ + D T+L++GQ ++P WK ++ ++I+++ H + IV Sbjct: 293 -IAKALTDWCDLAFVTKLVLGQSEQPRWKDYFKQSLIEQINHVPHHFKLEIV 343 Score = 130 bits (328), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 96/345 (27%), Positives = 165/345 (47%), Gaps = 26/345 (7%) Query: 29 IFIGAVSGSGKTYHMLREGTALQQQGIDVVICAVSTLQRPETIEQIGQLERIPSIHWVHD 88 +++G G GKTY ML G Q+G D+ I + QRPET + QL I + Sbjct: 1 MYLGYSPGVGKTYEMLSNGIDQYQEGADIKIGYIEPHQRPETNALVQQLPEISTSSRKFG 60 Query: 89 GTEKKDLNVEALIQRNPEVVLVDGLAHRNRPDAVHKTRLEDIHFLLGHGISVITTVNVYE 148 ++V +I+ NP+VVL+D LAH N H R +DI +L HGI V TT+N+ Sbjct: 61 SHTFHYIDVSKIIEANPDVVLIDELAHTNISKERHLKRYKDIEEILSHGIDVWTTLNIQH 120 Query: 149 LEGVRELAQKYMGIEVEETVSAHTLELADEIRLIDVTPETLLQRVSEGHVRN-----SKT 203 +E + GI+V E V + AD ++D++P L+QR+ G V + Sbjct: 121 IESLSGQIALMTGIQVTERVPDQFITSADAFEVVDISPNMLIQRLKAGKVYKKERLETAF 180 Query: 204 AALFQRGTLGVLRELALRLVAEGVNDSLEKHREQMGMLGPSGAAERILVSTQYHWNGSIY 263 + F L LREL LR VA+ ++ ++++ + + P A +GSIY Sbjct: 181 SNFFTYENLTELRELTLRTVADIMSQKGQQYKTKHTDITPHIAVA---------ISGSIY 231 Query: 264 ----VRRGQQVAKRLNGELIII---TFTNPKRPLTKEQAAFKESLVKLVRKVGAAFEELP 316 +R ++ A + + + + F P +++Q + + L + +GA E+ Sbjct: 232 NERVIREARRAAYKEHAKFTAVYIDVFETPSE--SRKQDHYVHKNLMLAKALGA---EIK 286 Query: 317 FHSRRRLPERLIQYALEHNVTRIMLGHSRQSRWQEFWQGSVVNDI 361 + + + L + VT+++LG S Q RW+++++ S++ I Sbjct: 287 VLYAQDIAKALTDWCDLAFVTKLVLGQSEQPRWKDYFKQSLIEQI 331 >SA1882 [T] COG2205 Osmosensitive K+ channel histidine kinase Length = 885 Score = 201 bits (510), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 121/362 (33%), Positives = 195/362 (53%), Gaps = 12/362 (3%) Query: 410 IGRIRRGRFKVFIGAAPGVGKTYTMLREGNHLLKKGVDVVIGLLETHGRQETGQQVAELP 469 IG+ +RG ++IG +PGVGKT+ ML L + VD+ IG +E H R ET +LP Sbjct: 9 IGK-KRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNVDIKIGYIEPHQRDETNALAEQLP 67 Query: 470 FIERAKIKYSETVLEEMDTGAILRRNPEVVLVDELAHTNVPGSKYKKRYEDVMEILNAGI 529 I K+ + +D I+ +P +VL+DELAHTN+ +++KRY D+ EILN GI Sbjct: 68 KITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDELAHTNISRDRHEKRYMDIEEILNHGI 127 Query: 530 SVISTVNVQHLESLNDAVAQITGVRVRETVPDSILRLADEVELIDVAPQALQARMKDGKI 589 V +T+N+QH+ESL+ + +TGV V+E VPD + AD +E++D++P+ L R+K GK+ Sbjct: 128 DVHTTLNIQHIESLSSQIELMTGVHVKERVPDYFIMSADVLEVVDISPEQLIKRLKAGKV 187 Query: 590 YAREKIDQALDNFFKIGNLIALRELSLREIADDVDERLEAWERVSSLRGPWNKQEVIFVC 649 Y ++++D A NFF +L LR L+LR +AD + ++ + + P I V Sbjct: 188 YKKDRLDVAFSNFFTYAHLSELRTLTLRTVADLMSDKEKVRHNHKTSLKPH-----IAVA 242 Query: 650 VKLSQSAGKLIRRGFRTAFRLKADWIVAYVHSAKELTEDELKMKASIENLTL--RLGGKF 707 + S +I+ F A + A + Y+ ++ + + K ++L L LG K Sbjct: 243 ISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFEKNRQYKDSQKQVHQHLMLAKSLGAKV 302 Query: 708 EMISSSGPSSRLAEVIQAKADEHHATQLIIGQCDRPAWKRLWEGTIIKKLLRTARHMDIL 767 +++ S + L E + + T+LIIGQ R W+ + +I L+ I Sbjct: 303 KVVYSQTVALGLDEWCKNQD----VTKLIIGQHIRNKWRDFFNTPLIDHLMSFEHSYKIE 358 Query: 768 IV 769 IV Sbjct: 359 IV 360 Score = 123 bits (309), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 93/350 (26%), Positives = 167/350 (47%), Gaps = 24/350 (6%) Query: 24 RGRLKIFIGAVSGSGKTYHMLREGTALQQQGIDVVICAVSTLQRPETIEQIGQLERIPSI 83 RG L I+IG G GKT+ ML L Q +D+ I + QR ET QL +I + Sbjct: 13 RGSLTIYIGYSPGVGKTFEMLSNAIELFQSNVDIKIGYIEPHQRDETNALAEQLPKITTN 72 Query: 84 HWVHDGTEKKDLNVEALIQRNPEVVLVDGLAHRNRPDAVHKTRLEDIHFLLGHGISVITT 143 H + L+V+ +I+ +P +VL+D LAH N H+ R DI +L HGI V TT Sbjct: 73 FTKHGSHHFQYLDVDRIIEESPTIVLIDELAHTNISRDRHEKRYMDIEEILNHGIDVHTT 132 Query: 144 VNVYELEGVRELAQKYMGIEVEETVSAHTLELADEIRLIDVTPETLLQRVSEGHVR---- 199 +N+ +E + + G+ V+E V + + AD + ++D++PE L++R+ G V Sbjct: 133 LNIQHIESLSSQIELMTGVHVKERVPDYFIMSADVLEVVDISPEQLIKRLKAGKVYKKDR 192 Query: 200 -NSKTAALFQRGTLGVLRELALRLVAEGVNDSLEKHREQMGMLGPSGAAERILVSTQYHW 258 + + F L LR L LR VA+ ++D + L P A Sbjct: 193 LDVAFSNFFTYAHLSELRTLTLRTVADLMSDKEKVRHNHKTSLKPHIAVA---------I 243 Query: 259 NGSIY----VRRGQQVAKRLNGEL--IIITFTNPKRPLTKEQAAFKESLVKLVRKVGAAF 312 +GSIY ++ +A++ + + I I R Q + L+ L + +GA Sbjct: 244 SGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFEKNRQYKDSQKQVHQHLM-LAKSLGAKV 302 Query: 313 EELPFHSRRRLPERLIQYALEHNVTRIMLGHSRQSRWQEFWQGSVVNDII 362 + + + + L ++ +VT++++G +++W++F+ +++ ++ Sbjct: 303 KVV---YSQTVALGLDEWCKNQDVTKLIIGQHIRNKWRDFFNTPLIDHLM 349 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 57,261,048 Number of extensions: 2410636 Number of successful extensions: 5396 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 5388 Number of HSP's successfully gapped: 8 Length of query: 788 Length of database: 38,378,431 Length adjustment: 114 Effective length of query: 674 Effective length of database: 25,230,355 Effective search space: 17005259270 Effective search space used: 17005259270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 120 (50.8 bits)