BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0149.0018.p (384 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl618 [R] COG4225 Predicted unsaturated glucuronyl hydrolase in... 186 4e-47 BS_yesR [R] COG4225 Predicted unsaturated glucuronyl hydrolase i... 177 3e-44 BH1120 [R] COG4225 Predicted unsaturated glucuronyl hydrolase in... 165 1e-40 AGl3363 [R] COG4225 Predicted unsaturated glucuronyl hydrolase i... 62 1e-09 >AGl618 [R] COG4225 Predicted unsaturated glucuronyl hydrolase involved in regulation of bacterial surface properties, and related proteins Length = 530 Score = 186 bits (473), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 14/317 (4%), Positives = 43/317 (13%), Gaps = 6/317 (1%) Query: 59 MNIENWDWVPGVGVIALLEYYESSGDPEVLVFLTEWVARNKHKAEETRVINSIAPYAVFP 118 +E + E++ A+ R + Sbjct: 74 RRLERAGLSWPLAEDVSDAVLEAALYKAAGTKTGHRRIAEPDWAQVHRELKRKHVTLQIL 133 Query: 119 ALYRLTGDEWLKEEAVRIADWLLKEAPKTREQALEHTVTEKADFSEQVWADTIFMAVLFL 178 + + + +H EK + Sbjct: 134 WDEYIGLHPEGYRYSRYCDLYRHWALKLPVTMRQDHAAGEKLFVDY-AGDTVSVVIDRLS 192 Query: 179 ARTAKLVENKSYAEEALKQVLIHLRLLNDKEHHVLFHGWNCNSGDHMSGARWTRANAWIA 238 +T + + + G + A + Sbjct: 193 GKTRQAHVFVGVLGASSLSFAHARWSGTLPDWIECHLLALETFGGAPALLVPDNAKVAVI 252 Query: 239 AGVPLIVKEIAPLIAIPDE--LGQRYGCLLAGLVSFQQKDGLWSTVMDQPGFYREISGSA 296 ++ + + V Sbjct: 253 KACHFDP-QVNRTYTGMATHYGSAVLPTRPRRPRDKAKVEAAVRIVERWLLGRLRHRVFY 311 Query: 297 GIAYGLQIAMEQGLVDASPVYESAAERALQAILPYISEAGVVDGVSGGTPVMDSVEAYNR 356 +A + E + + + R Sbjct: 312 SLADVNKAIGELLHDLND--KRVLRRIGRTRRQLFEELDYPALRPLPVERYVFAEWKIRR 369 Query: 357 IPVYPALYGQGLVLMLL 373 + + + Sbjct: 370 AGLDYHVDIDKHFYSVP 386 >BS_yesR [R] COG4225 Predicted unsaturated glucuronyl hydrolase involved in regulation of bacterial surface properties, and related proteins Length = 344 Score = 177 bits (448), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 109/318 (34%), Positives = 165/318 (51%), Gaps = 20/318 (6%) Query: 64 WDWVPGVGVIALLEYYESSGDPEVLVFLTEWVARNKHKAEETRVINSIAPYAVFPALYRL 123 WDW GV + E YE++ + E + L WV IN ++ L+++ Sbjct: 29 WDWPGGVAFYGVAEAYEATENEEYINLLKTWVDEQLEDGLPPLSINGVSIGHTLLFLHKV 88 Query: 124 TGDEWLKEEAVRIADWLLKEAPKTREQALEHTVTE-KADFSEQVWADTIFMAVLFLARTA 182 TGD+ E A +A+++L +AP+ E L+HTV + F EQ WADT+ MA LF+ R Sbjct: 89 TGDDVYLETAAEMAEYVLHKAPRFGEGILQHTVNAAEYVFPEQAWADTLMMAGLFMLRIG 148 Query: 183 KLVENKSYAEEALKQVLIHLRLLNDKEHHVLFHGWNCNSGDHMSGARWTRANAW----IA 238 +++E + Y E+ L+Q H +L D ++ +H W+ + +H+SG W RAN W +A Sbjct: 149 RVMEREDYFEDGLRQFHGHEDVLQDPVTNLYYHAWDNKAQNHLSGIYWGRANGWAALTMA 208 Query: 239 AGVPLIVKEIAPLIAIPDELGQRYGCLLAGLVSFQQKDGLWSTVMDQPGFYREISGSAGI 298 +PLI + I L + L+ LV Q + GLW T++D P Y E+S SAGI Sbjct: 209 KALPLIEVTHPSFMIIDGSLRDQ----LSALVRLQDESGLWHTILDDPDSYLEVSASAGI 264 Query: 299 AYGLQIAMEQGLVDASPVYESAAERALQAILPYISEAGVVDGVSGGTPVMDSVEAYNRIP 358 A L+ + +Y +++L AIL + E G V VS GT VM + E Y ++P Sbjct: 265 A--------SALMSSGKLYTKYVQKSLAAILDAVEEDGRVSRVSAGTAVMKNAEGYKQVP 316 Query: 359 VYPAL--YGQGLVLMLLA 374 Y + +GQGL L LA Sbjct: 317 -YKRIQGWGQGLALTFLA 333 >BH1120 [R] COG4225 Predicted unsaturated glucuronyl hydrolase involved in regulation of bacterial surface properties, and related proteins Length = 358 Score = 165 bits (417), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 113/328 (34%), Positives = 160/328 (48%), Gaps = 17/328 (5%) Query: 64 WDWVPGVGVIALLEYYESSGDPEVLVFLTEWVARNKHKAEETRVINSIAPYAVFPALYRL 123 WDW GV + YE++ L L EW +N ++ LY+ Sbjct: 29 WDWPAGVAFYGVGLAYEATKKDAYLQRLKEWFDEYMDYGIPPMCVNIVSMGHTLLTLYQH 88 Query: 124 TGDEWLKEEAVRIADWLLKEAPKTREQALEHTVTEKAD-FSEQVWADTIFMAVLFLARTA 182 TG+E + A+ AD+LL +AP+ E +HTV F EQ W DT+FMA FL R Sbjct: 89 TGEEKYLQLAIEKADYLLHDAPRFAEGVFQHTVGGGVSVFPEQAWVDTLFMAGYFLIRMG 148 Query: 183 KLVENKSYAEEALKQVLIHLRLLNDKEHHVLFHGWNCNSGDHMSGARWTRANAW----IA 238 + N++Y +E ++Q H + L D E + +HGW+ S +MSG W R NAW +A Sbjct: 149 DALNNQAYMDEGIRQFRGHEKYLQDPETNFYYHGWDHLSQSNMSGIFWARGNAWAAYTMA 208 Query: 239 AGVPLI-VKEIAPLIAIPDELGQRYGCLLAGLVSFQQKDGLWSTVMDQPGFYREISGSAG 297 A + +I VKE A + E+ L++ L++ Q + GLW T++ P Y E SGSAG Sbjct: 209 ASLRMIDVKEPAFM-----EIHGSLRDLMSALLNVQSEQGLWHTILTDPSSYEETSGSAG 263 Query: 298 IAYGLQIAMEQGLVDASPVYESAAERALQAILPYISEAGVVDGVSGGTPVMDSVEAYNRI 357 IA L + QG +P AA+RA A+L + G V GVS GT VM+ + Y Sbjct: 264 IAAALVL---QGA--NTPKGHHAAQRAFDALLEKVDARGTVLGVSTGTAVMNHADGYRMT 318 Query: 358 PVYPAL-YGQGLVLMLLAKAPGRKESQE 384 P +GQGL L + R E Sbjct: 319 PNRRIEGWGQGLALTFFSHVLMRYTETE 346 >AGl3363 [R] COG4225 Predicted unsaturated glucuronyl hydrolase involved in regulation of bacterial surface properties, and related proteins Length = 299 Score = 62.4 bits (150), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 12/213 (5%), Positives = 30/213 (14%), Gaps = 28/213 (13%) Query: 103 EETRVINSIAPYAVFPALYRLTGDEWLKEEAVRIADWLLKEAPKTREQALEHTVTEKADF 162 R + + G L V + + + Q Sbjct: 73 PALRDLREELLRLQEA-RETIAGVVRLTMIPVAFESIV-RPMLPSFCQRYPD----VVFE 126 Query: 163 SEQVWADTIFMAVLFLARTAKLVENKSYAEEALKQVLIHLRLLNDKEHHVLFHGWN---- 218 + F + + + + Sbjct: 127 ISTSERLDDIVTEGFDGGIRFGTLIDKDMVALPLTASTPIVIAGSASY-FENYPIPLAPE 185 Query: 219 ---CNSGDHMSGARWTRANAWIAAGVPLIVKEIAPLIAIPD-----ELGQRYGCLLAGLV 270 + +R W A D + G + + Sbjct: 186 HLAGHRCILYRYLSSSRLFRWPLAKG------SQKFEYKGDPALILDDGAAIRSAVMDGL 239 Query: 271 SFQQKDGLWSTVMDQPGF---YREISGSAGIAY 300 + G + + Sbjct: 240 GIGFLLRSQVASDLEAGNLREVLTDWLTDLPGF 272 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.134 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,889,099 Number of extensions: 1164693 Number of successful extensions: 2319 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 2305 Number of HSP's successfully gapped: 4 Length of query: 384 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 276 Effective length of database: 25,922,359 Effective search space: 7154571084 Effective search space used: 7154571084 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)