BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0147.0025.p (476 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1154_2 [T] COG0642 Signal transduction histidine kinase 86 2e-16 BH0819 [T] COG0642 Signal transduction histidine kinase 84 7e-16 BS_ybdK [T] COG0642 Signal transduction histidine kinase 77 5e-14 lin2515 [T] COG0642 Signal transduction histidine kinase 74 3e-13 Cgl0732 [T] COG0642 Signal transduction histidine kinase 72 2e-12 >BH1154_2 [T] COG0642 Signal transduction histidine kinase Length = 274 Score = 85.5 bits (210), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 66/221 (29%), Positives = 117/221 (52%), Gaps = 25/221 (11%) Query: 271 VSDLSHDLRTPLTVIRSHLHILGKEQLSGRGQESLKLMDERIENLGVLIENLLSYNLLSS 330 ++++SHDLRTPLT I ++ +L R + ++++D + L +LI++L + +SS Sbjct: 60 ITNVSHDLRTPLTSIITYTELLKDADEQERA-DYIEIIDRKARRLKILIDDLFEASKMSS 118 Query: 331 GRMTLKPERKDILRLLRESAAAWYPLWEKEGFRIEIDLEGEPLYWNVDESWFRRILDNLF 390 G + L E D+L+LL+++ A + E+ ++ I + EPLY D R+ DNL Sbjct: 119 GNIELTLETVDVLQLLQQALAEYGEALEQSSLQLRISVPEEPLYIKADGQKIWRVFDNLI 178 Query: 391 QNIVRHARSGLYVGVAVEDREGERVIVIRDHGQ-GLGS-----------GSESK---GAG 435 QNI++++ V ++V++++ I+I++ + LG G +S+ G+G Sbjct: 179 QNILKYSLEHTRVYLSVKEKKETVEIIIKNVAKYELGDNVDELLERFKRGDQSRNTEGSG 238 Query: 436 LGL----SIVDLLLKRMELGWKVDSTKQGTTIVILSLPHGN 472 LGL SIVDL +++ D K VI+ LP N Sbjct: 239 LGLAIAKSIVDLHEGYLDIDVDGDLFK-----VIIQLPMTN 274 >BH0819 [T] COG0642 Signal transduction histidine kinase Length = 309 Score = 83.6 bits (205), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 60/250 (24%), Positives = 107/250 (42%), Gaps = 29/250 (11%) Query: 216 ETAMAFTGKDGVPQLIDPGKPDEIGRLETAFNTMVTXXXXXXXXXXXXXXXXXXXVSDLS 275 ET M FTG + + +L+ A N ++ ++++S Sbjct: 47 ETVMVFTGDEQLRELL------------RAINDLIEEHNQVTATYQRTELSMRKMLANIS 94 Query: 276 HDLRTPLTVIRSHLHILGKEQLSGRGQESLKLMDERIENLGVLIENLLSYNLLSSGRMTL 335 HDL+TPLTVI ++ + + S +E L+ + + + LI L +G L Sbjct: 95 HDLKTPLTVIHGYVETILAGKGSANDRERLEKVQTKTIEIIELINKFFDLAKLEAGDQNL 154 Query: 336 KPERKDILRLLRESAAAWYPLWEKEGFRIEIDLEGEPLYWNVDESWFRRILDNLFQNIVR 395 ++ + L + +++ L + E ++EI++ +P Y DE RILDNL N +R Sbjct: 155 TLKKVAVNELCKNRILSYFDLIQNEKLQVEIEIPSQPFYVLADEKALDRILDNLISNAIR 214 Query: 396 HARSGLYVGVAVEDREGERVIVIRDHGQGLGSGSESK-----------------GAGLGL 438 + + G VG+ + + I + D G+G+ K G+GLGL Sbjct: 215 YGKDGKMVGLRLYHDDAYVSIEVWDRGKGISDKDREKVFERLYTLEDSRNKQYQGSGLGL 274 Query: 439 SIVDLLLKRM 448 +I L +R+ Sbjct: 275 TITKRLTERL 284 >BS_ybdK [T] COG0642 Signal transduction histidine kinase Length = 372 Score = 77.4 bits (189), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 14/248 (5%), Positives = 38/248 (15%), Gaps = 14/248 (5%) Query: 216 ETAMAFTGKDGVPQLIDPGKPDEIGRLETAFNTMVTXXXXXXXXXXXXXXXXXXXVSDLS 275 L G + Sbjct: 78 AMQKVVLQAATDHHLEICGGGTHPFQKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHV 137 Query: 276 HDLRTPLTVIRSHLHILGKEQLSGRGQESLKLMDERIENLGVLIE--NLLSYNLLSSGRM 333 H LH L + +L ++ ++ Sbjct: 138 HVGCASGDDAIYLLHGLSRF---VPHFIALSAASPYMQGTDTRFASSRPNIFSAFPDNGP 194 Query: 334 TLKPERKDILRLLRESAAAWYPLWEKEGFRIEIDLEGEPLYWNVDESWFRRILDNLFQNI 393 + Y + D+ P + V+ L Sbjct: 195 MPWVSNWQ--QFEALFRCLSYTTMIDSIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVN 252 Query: 394 VRHARSGLYVGVAVEDREGERVIVIRDHGQGLGSGSESKGAGLGLSIVDLLLKRMELGWK 453 + + +R ++ L + + + ++ + Sbjct: 253 MAGLIQA-TAHWLLTERP----FKHQEKDYLLYKFNRFQ--ACRYGLEGVITDPHTGDRR 305 Query: 454 VDSTKQGT 461 + Sbjct: 306 PLTEDTLR 313 >lin2515 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 74.3 bits (181), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 0/308 (0%), Positives = 0/308 (0%), Gaps = 28/308 (9%) Query: 182 MSLRFTLVMFVLFTGFMVVSLLFFIQIRKRLLRLE-TAMAFTGKDGVPQLIDPGKPDEIG 240 +R + + + Sbjct: 79 AVIRR-CRYELGVEITPP---ESIYPDFRYRATDPSGIVENEVCPVFAARTTSALQ-IND 133 Query: 241 RLETAFNTMVTXXXXXXXXXXXXXXXXXXXVSDLSHDLRTPLTVIRSHLHILGKEQLSGR 300 Sbjct: 134 -- 191 Query: 301 GQESLKLMDE----RIENLGVLIENLLSYNLLSSGRMTLKPERKDILRLLRESAAAWYPL 356 Sbjct: 192 251 Query: 357 WEKEGFRIEIDLEGEPLYWNVDESWFRRILDNLFQNIVRHARSGLYVGVAVEDREGERVI 416 Sbjct: 252 311 Query: 417 VIRDHGQ---------------GLGSGSESKGAGLGLSIVDLLLKRMELGWKVDSTKQGT 461 Sbjct: 312 - 370 Query: 462 TIVILSLP 469 Sbjct: 371 378 >Cgl0732 [T] COG0642 Signal transduction histidine kinase Length = 499 Score = 72.0 bits (175), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 70/264 (26%), Positives = 111/264 (42%), Gaps = 35/264 (13%) Query: 237 DEIGRLETAFNTMVTXXXXXXXXXXXXXXXXXXXVSDLSHDLRTPLTVIRSHLHILG--K 294 DE+ RL +FN M SD+SH+LRTPLT +R ++ + Sbjct: 223 DEMARLAVSFNAMAESLSAQITKLEEYGNLQRQFTSDVSHELRTPLTTVRMAADLIADSE 282 Query: 295 EQLSGRGQESLKLMDERIENLGVLIENLLSYNLLSSGRMTLKPERKDILRLLRESAAAWY 354 ++LS + + +LM+ ++ L+ +LL + +G L D+ +R + Sbjct: 283 DELSPGARRASQLMNRELDRFESLLSDLLEISRHDAGVAELSTALHDVRIPVRSALEQVQ 342 Query: 355 PLWEKEGFRIEIDLEGEPLYWNVDESWFRRILDNLFQNIVRHARSGLYVGVAVEDREGER 414 L + + ++L E + D RI+ NL N + H++ GL V + V D Sbjct: 343 HLATELDVELLVNLPEEAINIQGDSRRIERIIRNLLANAIDHSK-GLPVELKVADNVDAV 401 Query: 415 VIVIRDHGQGLGSGSE-----------------SKGAGLGLSIV--DLLLKRMELGWKVD 455 IV+ DHG GL G + S G GLGL+I D +L G +D Sbjct: 402 AIVVIDHGVGLKPGQDELVFNRFWRADPSRVRHSGGTGLGLAISREDAMLH----GGNLD 457 Query: 456 STKQGTT-------IVILSLPHGN 472 + GT +V+ PHGN Sbjct: 458 AA--GTIGVGSIFRLVLPKEPHGN 479 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,484,932 Number of extensions: 1283826 Number of successful extensions: 4746 Number of sequences better than 1.0e-05: 92 Number of HSP's gapped: 4762 Number of HSP's successfully gapped: 92 Length of query: 476 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 366 Effective length of database: 25,691,691 Effective search space: 9403158906 Effective search space used: 9403158906 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)