BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0147.0024.p (312 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0445 [V] COG1131 ABC-type multidrug transport system, ATPase c... 324 1e-88 BH0818 [V] COG1131 ABC-type multidrug transport system, ATPase c... 244 1e-64 BH0448 [V] COG1131 ABC-type multidrug transport system, ATPase c... 234 1e-61 lin0750 [V] COG1131 ABC-type multidrug transport system, ATPase ... 228 8e-60 BS_yhcH [V] COG1131 ABC-type multidrug transport system, ATPase ... 215 6e-56 >BH0445 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 306 Score = 324 bits (831), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 172/303 (56%), Positives = 224/303 (73%) Query: 10 TETVIETRDLLKEYRGRAAVEQLNLNIGKGEIYGFLGPNGAGKTTTIRMLLGLIKPTSGS 69 T +IET +L Y+ AV+++NL + +GEIYGFLGPNGAGKTTTIRMLLGLIK + GS Sbjct: 4 TNYLIETNNLTMTYKHVHAVDRINLKVKEGEIYGFLGPNGAGKTTTIRMLLGLIKASHGS 63 Query: 70 INIFGQDLKRNKLQILRKVGSLVESPSYYGHLSALDNLEAIRRILDVPKSRIAEVLEIVS 129 I IFG+DLK+++L IL+ +G+LVESPSYY HLS DNLE +R+I+ VPKSRIAEVLE+V Sbjct: 64 IKIFGKDLKQHRLDILKNIGALVESPSYYPHLSGEDNLETVRKIVKVPKSRIAEVLELVR 123 Query: 130 LTGEEKRPVKGFSLGMKQRLGIAAALLGSPELLILDEPTNGLDPSGIQEIRSLIKRLPVE 189 LT R V+ +SLGMKQRL IAAALL +P L+ILDEPTNGLDP+GI EIR+LI RLP E Sbjct: 124 LTKVADRKVQEYSLGMKQRLAIAAALLANPRLVILDEPTNGLDPAGIIEIRNLILRLPKE 183 Query: 190 EGITVLVSSHLLSEIEQMAGTVGIIRAGRMVYQDTIAQLQQQAAGDLRLAVSEPEEALIL 249 GITVL+SSHLLSE+EQ+A VGII G++++QD+I +L+++A + L+ S PE A + Sbjct: 184 YGITVLLSSHLLSEVEQVASQVGIIAKGKLIFQDSIEELRKKANRKIFLSTSHPEVAATI 243 Query: 250 ANRRGCGGTLQEGRITLPRMSDVRVALLIKELVENGHAIYRVEEHRQSLEEFFLQVVEGG 309 RG Q + + SD +A L+K VE +YR+ + SLEE FLQ+ +GG Sbjct: 244 LQERGINVDTQARGLVMNDRSDEEIAQLVKAFVEKDIDVYRISDETSSLEEIFLQLTKGG 303 Query: 310 QSV 312 Q++ Sbjct: 304 QTL 306 >BH0818 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 314 Score = 244 bits (624), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 135/303 (44%), Positives = 192/303 (63%), Gaps = 5/303 (1%) Query: 13 VIETRDLLKEYRGRAAVEQLNLNIGKGEIYGFLGPNGAGKTTTIRMLLGLIKPTSGSINI 72 VI TR+L K ++G+ + LNL++ KGEIYGFLGPNGAGKTT ++ML+ +KP++G I + Sbjct: 4 VITTRNLTKTFQGKEIISNLNLHVNKGEIYGFLGPNGAGKTTVMKMLMNFVKPSAGEIEL 63 Query: 73 FGQDLKRNKLQILRKVGSLVESPSYYGHLSALDNLEAIRRILDVPK-SRIAEVLEIVSLT 131 FGQ L ++L+++GS++E P +Y LSA +NLE + K S I E LE+V L Sbjct: 64 FGQRLTSQSYEMLKRIGSMIEFPIFYEKLSARENLELHCEYMGYYKPSAIEEALEMVKLQ 123 Query: 132 GEEKRPVKGFSLGMKQRLGIAAALLGSPELLILDEPTNGLDPSGIQEIRSLIKRLPVEEG 191 + VK FSLGMKQRLGIA A+ PELLILDEP NGLDP GI+EIR L + L E G Sbjct: 124 LTGSKAVKDFSLGMKQRLGIARAICTKPELLILDEPINGLDPVGIKEIRQLFQTLCNEYG 183 Query: 192 ITVLVSSHLLSEIEQMAGTVGIIRAGRMVYQDTIAQLQQQAAGDLRLAVSEPEEAL-ILA 250 +T+L+SSH+LSEIEQ+A TVG+IR G +V + + ++ Q + L + +AL +L Sbjct: 184 VTILISSHILSEIEQIADTVGVIRNGMLVAEVALDDIRSQQTEYIELLTYDARKALYVLD 243 Query: 251 NRRGCGGTLQEGRITLPRMSDVRVA--LLIKELVENGHAIYRVEEHRQSLEEFFLQVVEG 308 N G + +L R+ D + L K L+ N I + SLE++F+ ++EG Sbjct: 244 NELGLKNVKLMDQ-SLIRIYDHHLTQNQLSKTLISNDVNIEAIARKNHSLEDYFMNLIEG 302 Query: 309 GQS 311 ++ Sbjct: 303 DEN 305 >BH0448 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 307 Score = 234 bits (598), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 144/312 (46%), Positives = 208/312 (66%), Gaps = 20/312 (6%) Query: 13 VIETRDLLKEYRGRAAVEQLNLNIGKGEIYGFLGPNGAGKTTTIRMLLGLIKPTSGSINI 72 +IE + L K Y V+ ++L + +GE++GFLGPNG+GKTT +RMLLGL+K G I I Sbjct: 2 IIEAKGLKKIYNHVPVVDDVDLLVPEGEVFGFLGPNGSGKTTVMRMLLGLVKTDGGDIKI 61 Query: 73 FGQDLKRNKLQILRKVGSLVESPSYYGHLSALDNLE---AIRRILDVPKSRIAEVLEIVS 129 G+ L++N+L IL++VG+L+E+P+YYGHL+A+DNL+ +R+I D S+I + L+ V Sbjct: 62 LGKSLEKNRLSILKEVGALIETPAYYGHLNAIDNLKIAATLRQIED--HSQIEKTLDRVG 119 Query: 130 LTGEEKRPVKGFSLGMKQRLGIAAALLGSPELLILDEPTNGLDPSGIQEIRSLIKRLPVE 189 LT + + VK FSLGMKQRLGIA ALL P+LL+LDEPTNGLDP GIQE+R LI+ LP + Sbjct: 120 LTSSKHKKVKSFSLGMKQRLGIAMALLHQPKLLLLDEPTNGLDPEGIQEMRELIRDLPKK 179 Query: 190 EGITVLVSSHLLSEIEQMAGTVGIIRAGRMVYQDTIAQLQQQAAGDLRLAVSEPE----E 245 EG+TV VSSHLL EI+Q+A VG+I G ++YQ L G ++V PE E Sbjct: 180 EGVTVFVSSHLLGEIDQVASQVGVIYHGSLLYQGQKNDLLASHGG-TTVSVHTPEKDKVE 238 Query: 246 ALILAN----RRGCGGTLQEGRITLPRMS-DVRVALLIKELVENGHAIYRVEEHRQSLEE 300 L++ ++ E IT P ++ +V V LL +E ++N Y V+EH ++LE+ Sbjct: 239 QLLVMEGFHVQKSIRHKEDEIMITGPHVTKEVIVMLLQREGIQN----YYVQEHHRTLED 294 Query: 301 FFLQVVEGGQSV 312 ++ ++ GG+ V Sbjct: 295 AYMALI-GGKGV 305 >lin0750 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 301 Score = 228 bits (582), Expect = 8e-60, Method: Compositional matrix adjust. Identities = 133/308 (43%), Positives = 193/308 (62%), Gaps = 12/308 (3%) Query: 9 MTETVIETRDLLKEYRGRAAVEQLNLNIGKGEIYGFLGPNGAGKTTTIRMLLGLIKPTSG 68 MTE ++ +L K+ R R ++ ++ + GE++GFLGPNGAGKTTTIRM++GLIKPTSG Sbjct: 1 MTELALQVTNLHKKIRKREIIKGISFEVRPGEVFGFLGPNGAGKTTTIRMIVGLIKPTSG 60 Query: 69 SINIFGQDLKRNKLQILRKVGSLVESPSYYGHLSALDNLEAIRRI-LDVPKSRIAEVLEI 127 +I I G+D+++N + +R +GS+VE+P +Y L+ +NL R+ + K RI EV E+ Sbjct: 61 TILIGGKDIRKNFTEAMRGLGSIVENPEFYTFLTGQENLAYFARMDPSIKKERIQEVTEL 120 Query: 128 VSLTGEEKRPVKGFSLGMKQRLGIAAALLGSPELLILDEPTNGLDPSGIQEIRSLIKRLP 187 V L V +SLGM+QRLGIA ALL SP+LLILDEPTNGLDPSGI E+R I+ L Sbjct: 121 VGLEKRINDRVSTYSLGMRQRLGIAQALLSSPKLLILDEPTNGLDPSGIHEMRDFIRALA 180 Query: 188 VEEGITVLVSSHLLSEIEQMAGTVGIIRAGRMVYQDTIAQLQQQAAGDLRLAVSEPEEAL 247 EGI+VLVSSHLLSEIE + V I+ G ++ D ++ L A LR + E+A Sbjct: 181 RNEGISVLVSSHLLSEIELLCDRVAIMTDGTIIKTDQVSHLLSSRA-QLRWLATPFEQA- 238 Query: 248 ILANRRGCGGTLQEGRIT---LPRMSDVRVALLIKELVENGHAIYRVEEHRQSLEEFFLQ 304 + ++ E + L + R A ++LV G ++ +++ + SLE+ FL+ Sbjct: 239 -----KAFLASVTEVEVDGEYLVTAVNERSAEWNEQLVAKGIQVHEIDKKKPSLEDLFLE 293 Query: 305 VVEGGQSV 312 + GG S+ Sbjct: 294 LT-GGHSI 300 >BS_yhcH [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 154 Score = 215 bits (548), Expect = 6e-56, Method: Compositional matrix adjust. Identities = 0/296 (0%), Positives = 0/296 (0%), Gaps = 4/296 (1%) Query: 11 ETVIETRDLLKEYRGRAAVEQLNLNIGKGEIYGFLGPNGAGKTTTIRMLLGLIKPTSGSI 70 + + G + ++ + Sbjct: 2 MMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDNIFMNVMTFNTQSPVEKK 61 Query: 71 NIFGQDLKRNKLQILRKVGSLVESPSYYGHLSALDNLEAIRRILD-VPKSRIAEVLEIVS 129 + +L + + L + + + + I + + Sbjct: 62 AELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQLCSTIDNEQAIILKPGMFA 121 Query: 130 LTGEEKRPVKGFSLGMKQRLGIAAALLGSPELLILDEPTNGLDPSGIQEIRSLIKRLPVE 189 Sbjct: 122 181 Query: 190 EGITVLVSSHLLSEIEQMAGTVGIIRAGRMVYQDTIAQLQQQAAGDLRLAVSEPEEALIL 249 Sbjct: 182 241 Query: 250 ANRRGCGGTLQEGRITLPRMSDVRVALLIKELVENGHAIYRVEEHRQSLEEFFLQV 305 Sbjct: 242 --- 294 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.137 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,344,303 Number of extensions: 957562 Number of successful extensions: 10191 Number of sequences better than 1.0e-05: 2153 Number of HSP's gapped: 7691 Number of HSP's successfully gapped: 2378 Length of query: 312 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 206 Effective length of database: 26,153,027 Effective search space: 5387523562 Effective search space used: 5387523562 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)