BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0147.0017.p (675 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_mcpA [NT] COG0840 Methyl-accepting chemotaxis protein 197 6e-50 BS_tlpB [NT] COG0840 Methyl-accepting chemotaxis protein 194 3e-49 BS_mcpB [NT] COG0840 Methyl-accepting chemotaxis protein 193 9e-49 BS_tlpA [NT] COG0840 Methyl-accepting chemotaxis protein 192 1e-48 BH0876_2 [NT] COG0840 Methyl-accepting chemotaxis protein 187 6e-47 >BS_mcpA [NT] COG0840 Methyl-accepting chemotaxis protein Length = 661 Score = 197 bits (500), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 143/507 (28%), Positives = 265/507 (52%), Gaps = 12/507 (2%) Query: 170 LIVPVVDKKQQFSGAIAFSVTPDILNEMSKSIHVAESGYGYVVSGKGDYYAYSDMSRIGK 229 ++V + + + SG IA ++T + L + +K +++ GY ++++ A+ + + Sbjct: 164 MVVTIAQQTKDGSGVIAINMTIENLLKTTKKVNIGTQGYAFIMTKDKKVVAHPNEQSGTE 223 Query: 230 NMADYAKEPELKKAVTAMLSRESGSETYTGEDGKTIITYYKTIPGTDWKLMINVPKSEIF 289 D+ + MLS + G YT DG + T T WK+ + EI Sbjct: 224 LKGDW---------LDKMLSADKGDFQYT-MDGDKKKMAFDTNKLTGWKIGGTMYLDEIH 273 Query: 290 AKVNAAQRXXXXXXXXXXXXXXXXXXYLTRMVVKPLVAISGVMKRVAEGHLSERVEVRSG 349 + R + PL + G KR++EG L+E +++RS Sbjct: 274 EAAQPVLHLALIVLAAAIIIGIIVMTLIIRSITTPLKQLVGSSKRISEGDLTETIDIRSK 333 Query: 350 DEIGQMSKSINDTVSSLSGIVGTIDATVLQVAAAAEGLLEYANHSSNTSAEIATVIQEVA 409 DE+G++ KS N+ SSL ++ I +V VAA++E L A +S + I I++ + Sbjct: 334 DELGELGKSFNNMASSLRSLIHAIQDSVDNVAASSEELTASAAQTSKATEHITLAIEQFS 393 Query: 410 QGMEEQFKGSEQSARATEEMAVGLQRIAESSVSVSDQAETVSAEVQNGYLEIQSTLEQMT 469 G E+Q + E +A +M GL +A++S ++D + + G + T+ QM Sbjct: 394 NGNEKQNENIETAAEHIYQMNDGLTNMAQASEVITDSSVQSTEIASEGGKLVHQTVGQMN 453 Query: 470 VITTTAGQTADLIQTLTQQSEHIGQIIDVISEIANQTGLLSLNASIEAARAGEHGRGFGV 529 VI + + +++ L +S+ I I+ VI+ IA+QT LL+LNA+IEAARAGE+GRGF V Sbjct: 454 VIDKSVKEAEQVVRGLETKSKDITNILRVINGIADQTNLLALNAAIEAARAGEYGRGFSV 513 Query: 530 VANEVKKLAERTNASIVDIVDLIKQIQASTTNAAQSMEKSI-AEIGDGMGKMKNVGISFE 588 VA EV+KLA ++ S +I LI +I N + M +S+ E+ G+ +SF+ Sbjct: 514 VAEEVRKLAVQSADSAKEIEGLIIEI-VKEINTSLGMFQSVNQEVQTGLDITDKTEMSFK 572 Query: 589 HIRSSIREVSMQIQDVSAINEEMSAGTEEITASVSDMLTIAKDSAENAQAVSYASSEQME 648 I +++ ++Q++SA +++SA +EE++ + + +I+K+S+ + Q ++ ++ EQ+ Sbjct: 573 RISEMTNQIAGELQNMSATVQQLSASSEEVSGASEHIASISKESSAHIQDIAASAEEQLA 632 Query: 649 LMKKVVDSAKSLNEMMSTLKGEIEKFR 675 M+++ SA++L+ M L+ ++F+ Sbjct: 633 SMEEISSSAETLSSMAEELRDMTKRFK 659 >BS_tlpB [NT] COG0840 Methyl-accepting chemotaxis protein Length = 83 Score = 194 bits (494), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 0/506 (0%), Positives = 0/506 (0%), Gaps = 10/506 (1%) Query: 170 LIVPVVDKKQQFSGAIAFSVTPDILNEMSKSIHVAESGYGYVVSGKGDYYAYSDMSRIGK 229 Sbjct: 164 223 Query: 230 NMADYAKEPELKKAVTAMLSRESGSETYTGEDGKTIITYYKTIPGTDWKLMINVPKSEIF 289 Sbjct: 224 ---------- 273 Query: 290 AKVNAAQRXXXXXXXXXXXXXXXXXXYLTRMVVKPLVAISGVMKRVAEGHLSERVEVRSG 349 Sbjct: 274 333 Query: 350 DEIGQMSKSINDTVSSLSGIVGTIDATVLQVAAAAEGLLEYANHSSNTSAEIATVIQEVA 409 Sbjct: 334 393 Query: 410 QGMEEQFKGSEQSARATEEMAVGLQRIAESSVSVSDQAETVSAEVQNGYLEIQSTLEQMT 469 Sbjct: 394 453 Query: 470 VITTTAGQTADLIQTLTQQSEHIGQIIDVISEIANQTGLLSLNASIEAARAGEHGRGFGV 529 Sbjct: 454 513 Query: 530 VANEVKKLAERTNASIVDIVDLIKQIQASTTNAAQSMEKSIAEIGDGMGKMKNVGISFEH 589 Sbjct: 514 573 Query: 590 IRSSIREVSMQIQDVSAINEEMSAGTEEITASVSDMLTIAKDSAENAQAVSYASSEQMEL 649 Sbjct: 574 633 Query: 650 MKKVVDSAKSLNEMMSTLKGEIEKFR 675 Sbjct: 634 659 >BS_mcpB [NT] COG0840 Methyl-accepting chemotaxis protein Length = 662 Score = 193 bits (490), Expect = 9e-49, Method: Compositional matrix adjust. Identities = 148/509 (29%), Positives = 261/509 (51%), Gaps = 16/509 (3%) Query: 170 LIVPVVDKKQQFSGAIAFSVTPDILNEMSKSIHVAESGYGYVVSGKGDYYAYSDMSRIGK 229 +++ + + + SG +A +T D L E K I V + GY ++ + Y A+ + K Sbjct: 165 MVITIAQELKDGSGVVAMDITIDKLLEQMKQIKVGKEGYAFIATKNKTYVAHKNHKAGEK 224 Query: 230 NMADYAKEPELKKAVTAMLSRESGSETYT--GEDGKTIITYYKTIPGTDWKLMINVPKSE 287 D+ V M + +SG YT ED K Y T T WK+ + E Sbjct: 225 LSGDW---------VAKMYANDSGELQYTLNNEDKKMT---YTTNELTGWKIAGTMYMDE 272 Query: 288 IFAKVNAAQRXXXXXXXXXXXXXXXXXXYLTRMVVKPLVAISGVMKRVAEGHLSERVEVR 347 I + ++ R + KPL + K ++ G L+E +E+ Sbjct: 273 IKDASKSVLTTGMIVLIASIVAGGILILFIVRSITKPLKRLVQSSKTISRGDLTETIEIH 332 Query: 348 SGDEIGQMSKSINDTVSSLSGIVGTIDATVLQVAAAAEGLLEYANHSSNTSAEIATVIQE 407 S DE+G++ +S N+ SL ++ I +V VAA++E L A +S + I I++ Sbjct: 333 SKDELGELGESFNEMGQSLRSLISAIQNSVNNVAASSEQLTASAGQTSKATEHITMAIEQ 392 Query: 408 VAQGMEEQFKGSEQSARATEEMAVGLQRIAESSVSVSDQAETVSAEVQN-GYLEIQSTLE 466 + G EEQ + E S+ M GLQ+++++S ++ +A S E+ G +Q T Sbjct: 393 FSNGNEEQSRKVESSSHQLNLMNEGLQQVSQTSSDIT-KASIQSTEIAGTGEKFVQQTGG 451 Query: 467 QMTVITTTAGQTADLIQTLTQQSEHIGQIIDVISEIANQTGLLSLNASIEAARAGEHGRG 526 QM I + Q +++ L +S+ I I+ VI+ IA+QT LL+LNA+IEAARAGE GRG Sbjct: 452 QMNSINQSVQQAEAVVKGLEGKSKDITSILRVINGIADQTNLLALNAAIEAARAGESGRG 511 Query: 527 FGVVANEVKKLAERTNASIVDIVDLIKQIQASTTNAAQSMEKSIAEIGDGMGKMKNVGIS 586 F VVA EV+KLA ++ S +I LI++I A + ++ E+ G+ N S Sbjct: 512 FSVVAEEVRKLAVQSADSAKEIEKLIQEIVAEIDTSLHMFKEVNQEVQSGLVVTDNTKES 571 Query: 587 FEHIRSSIREVSMQIQDVSAINEEMSAGTEEITASVSDMLTIAKDSAENAQAVSYASSEQ 646 F+ I S E++ ++Q +++ E++S ++ ++A+VS + ++K+S+ + Q ++ ++ EQ Sbjct: 572 FQSIFSMTNEIAGKLQTMNSTVEQLSDRSQHVSAAVSGIADVSKESSASIQDIAASAEEQ 631 Query: 647 MELMKKVVDSAKSLNEMMSTLKGEIEKFR 675 + M+++ SA +L +M L+ ++F+ Sbjct: 632 LASMEEISSSATTLAQMAEELRDLTKQFK 660 >BS_tlpA [NT] COG0840 Methyl-accepting chemotaxis protein Length = 83 Score = 192 bits (489), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 0/513 (0%), Positives = 0/513 (0%), Gaps = 24/513 (4%) Query: 170 LIVPVVDKKQQFSGAIAFSVTPDILNEMSKSIHVAESGYGYVVSGKGDYYAYSDMSRIGK 229 Sbjct: 164 ----- 218 Query: 230 NMADYAKEPELK-KAVTAMLSRESGSETYTGEDGKTIITYYKTIPGTDWKLMINVPKSEI 288 Sbjct: 219 ------ 272 Query: 289 FAKVNAAQRXXXXXXXXXXXXX---XXXXXYLTRMVVKPLVAISGVMKRVAEGHLSERVE 345 Sbjct: 273 --- 329 Query: 346 VRSGDEIGQMSKSINDTVSSLSGIVGTIDATVLQVAAAAEGLLEYANHSSNTSAEIATVI 405 Sbjct: 330 389 Query: 406 QEVAQGMEEQFKGSEQSARATEEMAVGLQRIAESSVSVSDQAETVSAEVQNGYLEIQSTL 465 Sbjct: 390 449 Query: 466 EQMTVITTTAGQTADLIQTLTQQSEHIGQIIDVISEIANQTGLLSLNASIEAARAGEHGR 525 Sbjct: 450 509 Query: 526 GFGVVANEVKKLAERTNASIVDIVDLIKQIQA---STTNAAQSMEKSIAEIGDGMGKMKN 582 Sbjct: 510 --- 566 Query: 583 VGISFEHIRSSIREVSMQIQDVSAINEEMSAGTEEITASVSDMLTIAKDSAENAQAVSYA 642 Sbjct: 567 626 Query: 643 SSEQMELMKKVVDSAKSLNEMMSTLKGEIEKFR 675 Sbjct: 627 659 >BH0876_2 [NT] COG0840 Methyl-accepting chemotaxis protein Length = 359 Score = 187 bits (474), Expect = 6e-47, Method: Compositional matrix adjust. Identities = 121/358 (33%), Positives = 207/358 (57%), Gaps = 1/358 (0%) Query: 319 RMVVKPLVAISGVMKRVAEGHLS-ERVEVRSGDEIGQMSKSINDTVSSLSGIVGTIDATV 377 +M+ KPL ++ V + VA+G L+ ERV++++ DEIGQ+++S +L G++ I Sbjct: 1 KMITKPLARLTKVARHVADGDLTTERVKIKNRDEIGQLNESFEAMKENLRGLIQRILQNA 60 Query: 378 LQVAAAAEGLLEYANHSSNTSAEIATVIQEVAQGMEEQFKGSEQSARATEEMAVGLQRIA 437 QVA ++E L A +S+ + + + I+ +++G + Q ++ S + EEM + + Sbjct: 61 DQVAVSSEQLSASAEQTSSATEQTTSAIESISRGADMQTSAAQTSVKTLEEMNQIVTEMT 120 Query: 438 ESSVSVSDQAETVSAEVQNGYLEIQSTLEQMTVITTTAGQTADLIQTLTQQSEHIGQIID 497 + + + A G ++ T+E M I + + IQTL+++SE I +I+D Sbjct: 121 DGAKQIESNANLTLTYANEGDTLVKETVENMQSIDESVKGSDQAIQTLSKRSEDIVEILD 180 Query: 498 VISEIANQTGLLSLNASIEAARAGEHGRGFGVVANEVKKLAERTNASIVDIVDLIKQIQA 557 VI IA QT LL+LNA+IEAARAGEHG+GF VVA EV+KLAE ++ S I DLI ++ Sbjct: 181 VIQSIAEQTNLLALNAAIEAARAGEHGKGFAVVAEEVRKLAEESSQSTKKIHDLIDEMVK 240 Query: 558 STTNAAQSMEKSIAEIGDGMGKMKNVGISFEHIRSSIREVSMQIQDVSAINEEMSAGTEE 617 T N+ + M E+ G+ F I +SI+ V+ Q++ ++ + E++ + E Sbjct: 241 ETNNSVEKMGLVKEEVSVGLKTAAQTSEKFHSIVASIQGVTSQVEAMNRVTREVAEKSTE 300 Query: 618 ITASVSDMLTIAKDSAENAQAVSYASSEQMELMKKVVDSAKSLNEMMSTLKGEIEKFR 675 + SV +M ++A++SAE + +VS + E + M++V SA L EM TL+ I++F+ Sbjct: 301 VATSVQNMASVAEESAEQSSSVSAVAEETLASMQEVTASANQLTEMAETLQEAIKQFK 358 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.310 0.124 0.322 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,884,339 Number of extensions: 1515607 Number of successful extensions: 11525 Number of sequences better than 1.0e-05: 806 Number of HSP's gapped: 5568 Number of HSP's successfully gapped: 818 Length of query: 675 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 562 Effective length of database: 25,345,689 Effective search space: 14244277218 Effective search space used: 14244277218 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 119 (50.4 bits)