BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0147.0016.p (310 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value L0018 [C] COG0039 Malate/lactate dehydrogenases 278 7e-75 ML0018 [M] COG0768 Cell division protein FtsI/penicillin-binding... 278 7e-75 BS_lctE [C] COG0039 Malate/lactate dehydrogenases 256 2e-68 lin0242 [C] COG0039 Malate/lactate dehydrogenases 254 8e-68 SA0232 [C] COG0039 Malate/lactate dehydrogenases 253 3e-67 >L0018 [C] COG0039 Malate/lactate dehydrogenases Length = 314 Score = 278 bits (712), Expect = 7e-75, Method: Compositional matrix adjust. Identities = 127/264 (48%), Positives = 188/264 (71%), Gaps = 1/264 (0%) Query: 1 VAIVGSGMVGSSCAYSMVNQAICDEIMMIDRTYDRAMAQALDLSHCMDFTGTRTKVYAGT 60 V ++G+G VG+S AYSM+NQ + +E+++ID D+A +ALDL + + V AG Sbjct: 8 VVVIGTGFVGTSIAYSMINQGLVNELVLIDVNQDKAEGEALDLLDGVSWGQENVIVRAGD 67 Query: 61 SSDCAGMDVVILTAGANPKPGQSRLDVLDAAAVITKDIVTEIMAGGFDGIFVIAANPVDI 120 DC D+V++TAG N KPGQSRLD+++ A I + IVT++M GFDGIFVIA+NPVDI Sbjct: 68 YKDCKNADIVVVTAGVNQKPGQSRLDLVNTNAKIMRSIVTQVMDSGFDGIFVIASNPVDI 127 Query: 121 VTYMVWKISGLPRHRVIGTGTSIDSSRLKTLLSEVFTIDPRSVNGYALGEHGESQFVAWS 180 +TY+ W+ SGL + R++GTGT++D++R + L+ IDPRSV+GY +GEHG+S+ WS Sbjct: 128 LTYVAWETSGLDQSRIVGTGTTLDTTRFRKELATKLEIDPRSVHGYIIGEHGDSEVAVWS 187 Query: 181 HVTIGGKPILQIMEQHRERFSTLDLEDIARKTKDAGWEIFTRKGSTHFGIGSALAFITRS 240 H T+GGKPIL+ + +++ + DL +++ K K+A +EI +K +T++GIG + A I ++ Sbjct: 188 HTTVGGKPILEFIVKNK-KIGVEDLSNLSNKVKNAAYEIIDKKQATYYGIGMSTARIVKA 246 Query: 241 ILNDEHKIIAVSAILDGEYGQSGV 264 ILN+E I+ VSA L GEYGQ GV Sbjct: 247 ILNNEQAILPVSAYLRGEYGQEGV 270 >ML0018 [M] COG0768 Cell division protein FtsI/penicillin-binding protein 2 Length = 314 Score = 278 bits (712), Expect = 7e-75, Method: Compositional matrix adjust. Identities = 127/264 (48%), Positives = 188/264 (71%), Gaps = 1/264 (0%) Query: 1 VAIVGSGMVGSSCAYSMVNQAICDEIMMIDRTYDRAMAQALDLSHCMDFTGTRTKVYAGT 60 V ++G+G VG+S AYSM+NQ + +E+++ID D+A +ALDL + + V AG Sbjct: 8 VVVIGTGFVGTSIAYSMINQGLVNELVLIDVNQDKAEGEALDLLDGVSWGQENVIVRAGD 67 Query: 61 SSDCAGMDVVILTAGANPKPGQSRLDVLDAAAVITKDIVTEIMAGGFDGIFVIAANPVDI 120 DC D+V++TAG N KPGQSRLD+++ A I + IVT++M GFDGIFVIA+NPVDI Sbjct: 68 YKDCKNADIVVVTAGVNQKPGQSRLDLVNTNAKIMRSIVTQVMDSGFDGIFVIASNPVDI 127 Query: 121 VTYMVWKISGLPRHRVIGTGTSIDSSRLKTLLSEVFTIDPRSVNGYALGEHGESQFVAWS 180 +TY+ W+ SGL + R++GTGT++D++R + L+ IDPRSV+GY +GEHG+S+ WS Sbjct: 128 LTYVAWETSGLDQSRIVGTGTTLDTTRFRKELATKLEIDPRSVHGYIIGEHGDSEVAVWS 187 Query: 181 HVTIGGKPILQIMEQHRERFSTLDLEDIARKTKDAGWEIFTRKGSTHFGIGSALAFITRS 240 H T+GGKPIL+ + +++ + DL +++ K K+A +EI +K +T++GIG + A I ++ Sbjct: 188 HTTVGGKPILEFIVKNK-KIGVEDLSNLSNKVKNAAYEIIDKKQATYYGIGMSTARIVKA 246 Query: 241 ILNDEHKIIAVSAILDGEYGQSGV 264 ILN+E I+ VSA L GEYGQ GV Sbjct: 247 ILNNEQAILPVSAYLRGEYGQEGV 270 >BS_lctE [C] COG0039 Malate/lactate dehydrogenases Length = 321 Score = 256 bits (655), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 116/264 (43%), Positives = 183/264 (69%), Gaps = 1/264 (0%) Query: 1 VAIVGSGMVGSSCAYSMVNQAICDEIMMIDRTYDRAMAQALDLSHCMDFTGTRTKVYAGT 60 VA++G+G VGSS A++++NQ I DE+++ID ++AM +DL H F K GT Sbjct: 9 VALIGAGFVGSSYAFALINQGITDELVVIDVNKEKAMGDVMDLPHGKAFGLQPVKTSYGT 68 Query: 61 SSDCAGMDVVILTAGANPKPGQSRLDVLDAAAVITKDIVTEIMAGGFDGIFVIAANPVDI 120 DC D+V + AGAN KPG++RL++++ I K IV+E+MA GFDGIF++A NPVDI Sbjct: 69 YEDCKDADIVCICAGANQKPGETRLELVEKNLKIFKGIVSEVMASGFDGIFLVATNPVDI 128 Query: 121 VTYMVWKISGLPRHRVIGTGTSIDSSRLKTLLSEVFTIDPRSVNGYALGEHGESQFVAWS 180 +TY WK SGLP+ RVIG+GT++DS+R + +LSE F P++V+ + +GEHG+++ WS Sbjct: 129 LTYATWKFSGLPKERVIGSGTTLDSARFRFMLSEYFGAAPQNVHAHIIGEHGDTELPVWS 188 Query: 181 HVTIGGKPILQIMEQHRERFSTLDLEDIARKTKDAGWEIFTRKGSTHFGIGSALAFITRS 240 H +GG P+ +++E++ + + +L+ I K+A + I +KG+T++G+ +LA IT++ Sbjct: 189 HANVGGVPVSELVEKN-DAYKQEELDQIVDDVKNAAYHIIEKKGATYYGVAMSLARITKA 247 Query: 241 ILNDEHKIIAVSAILDGEYGQSGV 264 IL++E+ I+ VS LDG+YG V Sbjct: 248 ILHNENSILTVSTYLDGQYGADDV 271 >lin0242 [C] COG0039 Malate/lactate dehydrogenases Length = 313 Score = 254 bits (650), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 122/264 (46%), Positives = 182/264 (68%), Gaps = 5/264 (1%) Query: 1 VAIVGSGMVGSSCAYSMVNQAICDEIMMIDRTYDRAMAQALDLSHCMDFTGTRTKVYAGT 60 + +VG G VGSS A++ VN +I E +ID DR + A+DLSH + F+ T K+Y+ Sbjct: 7 IILVGDGAVGSSYAFACVNLSIGQEFGIIDIDKDRTIGDAMDLSHAVPFS-TPKKIYSAN 65 Query: 61 SSDCAGMDVVILTAGANPKPGQSRLDVLDAAAVITKDIVTEIMAGGFDGIFVIAANPVDI 120 SDC D+V++TAG KPG++RLD+++ I K IV E+MA GFDGIF+IA+NPVDI Sbjct: 66 YSDCHDADLVVVTAGTAQKPGETRLDLVNRNIKIMKGIVDEVMASGFDGIFLIASNPVDI 125 Query: 121 VTYMVWKISGLPRHRVIGTGTSIDSSRLKTLLSEVFTIDPRSVNGYALGEHGESQFVAWS 180 +TY WK SGLP+ RVIG+GTS+D++R + +++ +D R+V+GY LGEHG++QF AWS Sbjct: 126 LTYATWKFSGLPKERVIGSGTSLDTARFRMSIADYLKVDARNVHGYILGEHGDTQFPAWS 185 Query: 181 HVTIGGKPILQIMEQHRERFSTLDLEDIARKTKDAGWEIFTRKGSTHFGIGSALAFITRS 240 H T+GG PI + + + + ++ I +DA +EI +KG+T +G+ +ALA IT++ Sbjct: 186 HTTVGGLPITEWINEDEQGA----MDTIFVSVRDAAYEIINKKGATFYGVAAALARITKA 241 Query: 241 ILNDEHKIIAVSAILDGEYGQSGV 264 ILN+E+ I+ +S LDG YG + + Sbjct: 242 ILNNENAILPLSVYLDGHYGMNDI 265 >SA0232 [C] COG0039 Malate/lactate dehydrogenases Length = 317 Score = 253 bits (646), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 118/264 (44%), Positives = 175/264 (66%), Gaps = 2/264 (0%) Query: 1 VAIVGSGMVGSSCAYSMVNQAICDEIMMIDRTYDRAMAQALDLSHCMDFTGTRTKVYAGT 60 V ++G+G VGSS A+S+VNQ+I DE+++ID ++ +DL H ++ T +V AG Sbjct: 9 VVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKVRGDVMDLKHATPYSPTTVRVKAGE 68 Query: 61 SSDCAGMDVVILTAGANPKPGQSRLDVLDAAAVITKDIVTEIMAGGFDGIFVIAANPVDI 120 SDC D+V++ AGA KPG++RLD++ I K IV E+MA FDGIF++A NPVDI Sbjct: 69 YSDCHDADLVVICAGAAQKPGETRLDLVSKNLKIFKSIVGEVMASKFDGIFLVATNPVDI 128 Query: 121 VTYMVWKISGLPRHRVIGTGTSIDSSRLKTLLSEVFTIDPRSVNGYALGEHGESQFVAWS 180 + Y WK SGLP+ RVIG+GT +DS+R + LLSE F + PRSV+ +GEHG+++ WS Sbjct: 129 LAYATWKFSGLPKERVIGSGTILDSARFRLLLSEAFDVAPRSVDAQIIGEHGDTELPVWS 188 Query: 181 HVTIGGKPILQIMEQHRERFSTLDLEDIARKTKDAGWEIFTRKGSTHFGIGSALAFITRS 240 H I G+P+ ++EQ E +E I +T+DA ++I KG+T++G+ LA IT + Sbjct: 189 HANIAGQPLKTLLEQRPE--GKAQIEQIFVQTRDAAYDIIQAKGATYYGVAMGLARITEA 246 Query: 241 ILNDEHKIIAVSAILDGEYGQSGV 264 I +E ++ VSA+L+GEY + V Sbjct: 247 IFRNEDAVLTVSALLEGEYDEEDV 270 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,495,171 Number of extensions: 793641 Number of successful extensions: 1525 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 1490 Number of HSP's successfully gapped: 26 Length of query: 310 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 204 Effective length of database: 26,153,027 Effective search space: 5335217508 Effective search space used: 5335217508 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)