BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0147.0004.p
         (155 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BS_yndN [E] COG0346 Lactoylglutathione lyase and related lyases        50   1e-06
BH1778 [E] COG0346 Lactoylglutathione lyase and related lyases         50   2e-06

>BS_yndN [E] COG0346 Lactoylglutathione lyase and related lyases
          Length = 144

 Score = 50.1 bits (118), Expect = 1e-06,   Method: Compositional matrix adjust.
 Identities = 36/125 (28%), Positives = 59/125 (47%), Gaps = 12/125 (9%)

Query: 2   IKGLYEAHLPVSNLEVSIEFYKKL-GLKFAWRDEETAFFWITEGKSWVGLWEGREV-QTP 59
           IKG+      VS+L+ SI+FY+K+ G K   +   TA+F +     W+ L E  ++ +  
Sbjct: 3   IKGINHLLFSVSHLDTSIDFYQKVFGAKLLVKGRTTAYFDM--NGIWLALNEEPDIPRND 60

Query: 60  YHPSLRHIAFQVTYEDLKHSLAWLESIQIEAVPFGNRSSVEPFIRPHQGNASVYFNDPDG 119
              S  HIAF +   + +   A L+ + +  +P   R          +   S+YF DPDG
Sbjct: 61  IKLSYTHIAFTIEDHEFEEMSAKLKRLHVNILPGRERDE--------RDRKSIYFTDPDG 112

Query: 120 NSLEL 124
           +  E 
Sbjct: 113 HKFEF 117


>BH1778 [E] COG0346 Lactoylglutathione lyase and related lyases
          Length = 141

 Score = 49.7 bits (117), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 43/144 (29%), Positives = 65/144 (45%), Gaps = 20/144 (13%)

Query: 2   IKGLYEAHLPVSNLEVSIEFYKK-LGLKFAWRDEETAFFWITEGKSWVGLWEGREV-QTP 59
           I+G+      V  LE SIEFYKK LG K   +   TA+F +     W+ L E  ++ +  
Sbjct: 3   IQGINHLLFSVKCLERSIEFYKKALGAKLLVKGRTTAYFDLQ--GIWLALNEEPDIPRNE 60

Query: 60  YHPSLRHIAFQVTYEDLKHSLAWLESIQIEAVPFGNRSSVEPFIRPHQGNASVYFNDPDG 119
            H S  HIAF V  E+++ +   L  + +  +    R          +   S+YF DPDG
Sbjct: 61  IHQSYTHIAFTVGEEEMEEAYERLAGLGVNILKGRPRDP--------RDRQSIYFTDPDG 112

Query: 120 NSLELMCFVEVPEELRTVTDKLSF 143
           +  E  C         T+ D+L +
Sbjct: 113 HKFEFHC--------GTLNDRLDY 128


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.318    0.136    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 12,631,380
Number of extensions: 486269
Number of successful extensions: 833
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 834
Number of HSP's successfully gapped: 3
Length of query: 155
Length of database: 38,378,431
Length adjustment: 97
Effective length of query: 58
Effective length of database: 27,191,033
Effective search space: 1577079914
Effective search space used: 1577079914
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 111 (47.4 bits)