BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0139.0003.p (420 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yqjW [L] COG0389 Nucleotidyltransferase/DNA polymerase involv... 361 1e-99 BH2741 [L] COG0389 Nucleotidyltransferase/DNA polymerase involve... 348 8e-96 BS_uvrX [L] COG0389 Nucleotidyltransferase/DNA polymerase involv... 178 1e-44 pli0067 [L] COG0389 Nucleotidyltransferase/DNA polymerase involv... 161 2e-39 lin2823 [L] COG0389 Nucleotidyltransferase/DNA polymerase involv... 159 5e-39 >BS_yqjW [L] COG0389 Nucleotidyltransferase/DNA polymerase involved in DNA repair Length = 673 Score = 361 bits (927), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 29/421 (6%), Positives = 69/421 (16%), Gaps = 10/421 (2%) Query: 1 MPKDRVILLSDCQAFYASVEKAAHPEYRDRPVAV-GDPSRMNGIVLAACPVAKAQGVTTA 59 M + + + A + R + A A A Sbjct: 1 MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAA 60 Query: 60 SRVGEALAKCPELIVIRPRMGTYIKVSLLISEIYRTYTDLVEPYSIDEQFLDVTGSLAYF 119 + E + + R M + L ++ D L Sbjct: 61 REMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKEL 120 Query: 120 GGSLKDMIQKIQHHVLLSTGVWTRIGIGSTKVMAKMATDNYAKKMKDGIYELTFNQLEST 179 L K+ L+ST + + + A A + + Sbjct: 121 TEGL-IEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYDAHLKACN 179 Query: 180 LWRLPVQDMFMVASRMTRNFWRMGISTIGDIARMELPEFKQRMRTTMGKQSDIQAEYYWQ 239 + + R ++ + S + Sbjct: 180 VLDFDDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDT--------NTSQYELVKLLV 231 Query: 240 TARGLDPSPVVTGIRHQIKSVGHGKALRWNLYTRLTDXXXXXXXXXXXXCQRARRYRYMG 299 +R + L + + + Sbjct: 232 GSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQNYRSSGRILKAANILI 291 Query: 300 SVVSISVLETDGESSCSYERQMTLPEPSSLTHEVAAAALALFMDNWSGLPVGRLAITLSR 359 + + L + + + AI Sbjct: 292 ANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAHHFVNKTQYKDYAILYRG 351 Query: 360 LSDDGVIQLTLFDDRARSYNKEHAIDGIKARYGNQALIRASSLLESGVARERAEQIGGHY 419 V + L +R + + + Sbjct: 352 NHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNPDDDSAFLRIVNTPKR 411 Query: 420 K 420 + Sbjct: 412 E 412 >BH2741 [L] COG0389 Nucleotidyltransferase/DNA polymerase involved in DNA repair Length = 673 Score = 348 bits (894), Expect = 8e-96, Method: Compositional matrix adjust. Identities = 28/418 (6%), Positives = 80/418 (19%), Gaps = 11/418 (2%) Query: 4 DRVILLSDCQAFYASVEKAAHPEYRDRPVAV-GDPSRMNGIVLAACPVAKAQGVTTASRV 62 F + + + + A T + Sbjct: 2 RLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAR 61 Query: 63 GEALAKCPELIVIRPRMGTYIKVSLLISEIYRTYTDLVEPYSIDEQFLDVTGSLAYFGGS 122 L R L +I + + + F D + Sbjct: 62 EMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELT 121 Query: 123 LKDMIQKIQHHVLLSTGVWTRIGIGSTKVMAKMATDNYAKKMKDGIYELTFNQLESTLWR 182 + +I+ + + + A+ + ++ Sbjct: 122 -EGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYDAHLKACNV 180 Query: 183 LPVQDMFMVASRMTRNFWRMGISTIGDIARMELPEFKQRMRTTMGKQSDIQAEYYWQTAR 242 L D+ ++ + + + + I + + + T Q E Sbjct: 181 LDFDDLILLPTLLLQANEEVRKRWQNKIRYLLV----DEYQDTNT----SQYELVKLLVG 232 Query: 243 GLDPSPVVTGIRHQIKSVGHGKALRWNLYTRLTDXXXXXXXXXXXXCQRARRYRYMGSVV 302 VV I S + R + ++ Sbjct: 233 SRARFTVVGDDDQSIYSWRGARPQNLV-LLSQDFPALKVIKLEQNYRSSGRILKAANILI 291 Query: 303 SISVLETDGESSCSYERQMTLPEPSSLTHEVAAAALALFMDNWSGLPVGRLAITLSRLSD 362 + + + L S+ E A + + + + Sbjct: 292 ANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAHHFVNKTQYKDYAILYRG 351 Query: 363 DGVIQLTLFDDRARSYNKEHAIDGIKARYGNQALIRASSLLESGVARERAEQIGGHYK 420 + ++ + + + A + + + A + Sbjct: 352 NHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNPDDDSAFLRIVNTP 409 >BS_uvrX [L] COG0389 Nucleotidyltransferase/DNA polymerase involved in DNA repair Length = 673 Score = 178 bits (452), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 26/420 (6%), Positives = 68/420 (16%), Gaps = 25/420 (5%) Query: 7 ILLSDCQAFYASVEKAA---HPEYRDRPVAVGDPSRMNGIVLAACPVAKAQ-GVTTASRV 62 ++ A + G + I A+ + Sbjct: 12 EFVTGPCLVLAGAGSGKTRVITNKIAHLI-RGCGYQARHIAAVTFTNKAAREMKERVGQT 70 Query: 63 GEALAKCPELIVIRPRMGTYIKVSLLISEIYRTYT--DLVEPYSIDEQFLDVTGSLAYFG 120 + L++ + + + + + G Sbjct: 71 LG-RKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGL----- 124 Query: 121 GSLKDMIQKIQHHVLLSTGVWTRIGIGSTKVMAKMATDNYAKKMKDGIYELTFNQLESTL 180 D + Q +S A D Sbjct: 125 -IEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYDAHLKACNVLDF 183 Query: 181 WRL-PVQDMFMVASRMTRNFWRMGISTIGDIARMELPEFKQRMRTTMGKQSDIQAEYYWQ 239 L + + + A+ R W+ I + + Q + S Sbjct: 184 DDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDT-NTSQYELVKLLVGS-----RA-- 235 Query: 240 TARGLDPSPVVTGIRHQIKSVGHGKALRWNLYTRLTDXXXXXXXXXXXXCQRARRYRYMG 299 + + + + A Sbjct: 236 RFTVVGDDDQSIYSWRGARPQNLVLLSQDF-PALKVIKLEQNYRSSGRILKAANILIANN 294 Query: 300 SVVSISVLETDGESSCSYERQMTLPEPSSLTHEVAAAALALFMDNWSGLPVGRLAITLSR 359 V L ++ + E F++ L + Sbjct: 295 PHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAHHFVNKTQYKDYAILYRGNHQ 354 Query: 360 LSDDGVIQLTLFD-DRARSYNKEHAIDGIKARYGNQALIRASSLLESGVARERAEQIGGH 418 + + + IK ++ + + + Sbjct: 355 SRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNPDDDSAFLRIVNTPKREIG 414 >pli0067 [L] COG0389 Nucleotidyltransferase/DNA polymerase involved in DNA repair Length = 426 Score = 161 bits (408), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 129/423 (30%), Positives = 204/423 (48%), Gaps = 20/423 (4%) Query: 5 RVILLSDCQAFYASVE--KAAHPEYRDRPVAVGDPSRMNGIVLAACP-VAKAQGVTTASR 61 R IL D ++F+ASVE K + V + + R G+VLAA P + K + T SR Sbjct: 13 RDILCVDVKSFFASVECVKRGLDPLQAFLVVMSNADRAGGLVLAASPQMKKVFRIKTGSR 72 Query: 62 VGEALAKCPELIVIRPRMGTYIKVSLLISEIYRTYT--DLVEPYSIDEQFLDVTGSLAYF 119 E P +I+ PRM Y+KV+ LI +I+ Y + YSIDE FLDVT S F Sbjct: 73 KFEVPEDDPRIIIAPPRMQLYLKVNELIQKIFLRYVSREDFHVYSIDEAFLDVTHSKKLF 132 Query: 120 GGSLKDMIQKIQHHVLLSTGVWTRIGIGSTKVMAKMATDNYAKKMKDGIYELTFNQLEST 179 G S + + +KIQ +L ++ +GIG ++AK+A DN AK K GI E + + T Sbjct: 133 G-SAEVIAKKIQADILKELKLYVTVGIGDNPLLAKLALDNEAKHQKSGIAEWRYKDVPET 191 Query: 180 LWRL-PVQDMFMVASRMTRNFWRMGISTIGDIARMELPEFKQRMRTTMGKQSDIQAEYYW 238 +W++ P+ D + + R RMGI +I +++M LP + +R +G+Q YY Sbjct: 192 VWKIRPITDFWGIGKRTAFQLERMGIYSIHGLSQM-LPSYLKRRLGVIGEQL-----YY- 244 Query: 239 QTARGLDPSPVVTGIRHQIKSVGHGKALRWNLYTRLTDXXXXXXXXXXXXCQRARRYRYM 298 A G+D S + + KS G + L + Y R R + Sbjct: 245 -HAHGIDYSRLSEKYKPIEKSYGKSQILEKD-YHDPNQIAIIIQEMVEEVAMRLRNHHVD 302 Query: 299 GSVVSISVLETDGESSCSYERQMTLPEPSSLTHEVAAAALALFMDNWSGLPVGRLAITLS 358 SV+ +S + + + Q + SS + E+ L LF V +A++ + Sbjct: 303 TSVIHLSCGYSRYSTKNGFSHQKKIMSTSS-SKELVPYFLELFWKYQENDAVRSVAVSCA 361 Query: 359 RLSDDGVIQLTLFDDRARSYNKEH---AIDGIKARYGNQALIRASSLLESGVARERAEQI 415 + +QL++F+D ++ +E ID I+ RYG +AL+ A+SL+ +RA + Sbjct: 362 GIKRKTSMQLSVFEDYTKTLQQEQLERTIDKIRERYGFKALMHANSLVAGATGLKRATLV 421 Query: 416 GGH 418 GGH Sbjct: 422 GGH 424 >lin2823 [L] COG0389 Nucleotidyltransferase/DNA polymerase involved in DNA repair Length = 673 Score = 159 bits (403), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 20/416 (4%), Positives = 62/416 (14%), Gaps = 26/416 (6%) Query: 15 FYASVEKAAHPEYRDRP-----VAVGDPSRMNGIVLAACPVAKAQG-VTTASRVGEALAK 68 + A + R G + Sbjct: 15 TGPCLVLAGA---GSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKERVGQTL 71 Query: 69 CPELIVIRPRMGTYIKVSLLISEIYRTYT--DLVEPYSIDEQFLDVTGSLAYFGGSLKDM 126 + + +I Y + +Q + + Sbjct: 72 GRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGLI-EDDKV 130 Query: 127 IQKIQHHVLLSTGVWTRIGIGSTKVMAKMATDNYAKKMKDGIYELTFNQLESTLWRL-PV 185 + + + + + + +A L + + Sbjct: 131 LLQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYDAHLKACNVLDFDDLILLP 190 Query: 186 QDMFMVASRMTRNFWRMGISTIGDIARMELPEFKQRMRTTMGKQSDIQAEYYWQTARGLD 245 + + + + + D + + + + D Sbjct: 191 TLLLQANEEVRKRWQNKIRYLLVDEYQDT----NTSQYELVK----LLVGSRARFTVVGD 242 Query: 246 PSPVVTGIRHQIKSVGHGKALRWNLYTRLTDXXXXXXXXXXXXCQRARRYRYMGSVVSIS 305 + R + + ++ V Sbjct: 243 DDQSIYSWRGARPQNLVLLSQDFP-ALKVIKLEQNYRSSGRILKAANILIANNPHVFEKR 301 Query: 306 VLETDGESSCSYERQMTLPEPSSLTHEVAAAALALFMDNWSGLPVGRLAITLSRLSDDGV 365 + G + E + F++ L + Sbjct: 302 LFSELGYGAELKVLSANNEEHEA-ERVTGELIAHHFVNKTQYKDYAILYRGNHQSRVFEK 360 Query: 366 IQLTLFDDRARSYNKEH---AIDGIKARYGNQALIRASSLLESGVARERAEQIGGH 418 + S Y + +IG Sbjct: 361 FLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNPDDDSAFLRIVNTPKREIGPA 416 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,886,708 Number of extensions: 1131595 Number of successful extensions: 2754 Number of sequences better than 1.0e-05: 42 Number of HSP's gapped: 2645 Number of HSP's successfully gapped: 42 Length of query: 420 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 311 Effective length of database: 25,807,025 Effective search space: 8025984775 Effective search space used: 8025984775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)