BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0137.0015.p (181 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2787 [I] COG0439 Biotin carboxylase 278 3e-75 BS_accC [I] COG0439 Biotin carboxylase 268 4e-72 SA1357 [I] COG0439 Biotin carboxylase 228 5e-60 AGc2451 [I] COG0439 Biotin carboxylase 217 7e-57 mll0203 [I] COG0439 Biotin carboxylase 211 6e-55 >BH2787 [I] COG0439 Biotin carboxylase Length = 452 Score = 278 bits (712), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 133/181 (73%), Positives = 148/181 (81%), Gaps = 1/181 (0%) Query: 2 NYSGAGTLEFLLG-PDGQFYFMEMNTRIQVEHPVTEMVTGVDLIKEMISVAEGNPLSFTQ 60 NYSGAGT+EF+ +G FYFMEMNTRIQVEHPVTEMVTG+DLIKE I VA G PLS+ Q Sbjct: 267 NYSGAGTVEFIYDHNNGDFYFMEMNTRIQVEHPVTEMVTGIDLIKEQIKVASGKPLSYKQ 326 Query: 61 EDIKINGWSMECRINAEDPERNFMPSPGKIGFYLPPGGLGVRVDSAAYPGYTISPFYDSM 120 ED+ NGWS+ECRINAE PE+NFMPSPGK+ YL PGGLGVRVDSAAYPGYTI PFYDSM Sbjct: 327 EDVTFNGWSIECRINAESPEKNFMPSPGKLKSYLAPGGLGVRVDSAAYPGYTIPPFYDSM 386 Query: 121 IAKLIVWAPTRREAIAKMKRALSEFAVEGIHTTISFHQKLLEHPVFLDGNFDIKFLEEYE 180 IAK+I TR EAIAKMKRAL+EF VEGI TTI FH +LL H F+ G+F+ KFLE Y Sbjct: 387 IAKVITHGATRDEAIAKMKRALAEFEVEGIETTIPFHLRLLNHETFVSGDFNTKFLEIYN 446 Query: 181 I 181 I Sbjct: 447 I 447 >BS_accC [I] COG0439 Biotin carboxylase Length = 450 Score = 268 bits (685), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 125/180 (69%), Positives = 147/180 (81%), Gaps = 1/180 (0%) Query: 3 YSGAGTLEFLLGPDGQ-FYFMEMNTRIQVEHPVTEMVTGVDLIKEMISVAEGNPLSFTQE 61 Y+GAGT+EF+ + Q +YFMEMNTRIQVEHPVTEMVTG DLIKE I VA G LS QE Sbjct: 268 YTGAGTVEFIYDYNEQRYYFMEMNTRIQVEHPVTEMVTGTDLIKEQIKVASGMELSLKQE 327 Query: 62 DIKINGWSMECRINAEDPERNFMPSPGKIGFYLPPGGLGVRVDSAAYPGYTISPFYDSMI 121 D++ GW++ECRINAE+P +NFMPSPG+I YLPPGGLGVRVDSAAYPGY+I P+YDSMI Sbjct: 328 DVEFEGWAIECRINAENPSKNFMPSPGEIKMYLPPGGLGVRVDSAAYPGYSIPPYYDSMI 387 Query: 122 AKLIVWAPTRREAIAKMKRALSEFAVEGIHTTISFHQKLLEHPVFLDGNFDIKFLEEYEI 181 AK+I + TR EAIA+MKRALSEF +EGI TTI FH KLLEH F+ G F+ KFLE Y++ Sbjct: 388 AKVITYGKTRDEAIARMKRALSEFVIEGIETTIPFHLKLLEHETFVSGEFNTKFLETYDV 447 >SA1357 [I] COG0439 Biotin carboxylase Length = 451 Score = 228 bits (580), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 113/182 (62%), Positives = 135/182 (74%), Gaps = 1/182 (0%) Query: 1 VNYSGAGTLEFLLG-PDGQFYFMEMNTRIQVEHPVTEMVTGVDLIKEMISVAEGNPLSFT 59 VNY AGT+EF+ D +FYFMEMNTRIQVEHPVTEMVTG+DL+K + VA G+ L + Sbjct: 265 VNYENAGTIEFIYDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYK 324 Query: 60 QEDIKINGWSMECRINAEDPERNFMPSPGKIGFYLPPGGLGVRVDSAAYPGYTISPFYDS 119 QEDIK+ G ++E RINAE+P +NFMPSPGKI YL PGG GVR++SA Y YTI P+YDS Sbjct: 325 QEDIKLTGHAIEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDS 384 Query: 120 MIAKLIVWAPTRREAIAKMKRALSEFAVEGIHTTISFHQKLLEHPVFLDGNFDIKFLEEY 179 M+AKLI+ PTR EAI RALSEF V GI TTI FH KLL + +F G F+ FLE+ Sbjct: 385 MVAKLIIHEPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQN 444 Query: 180 EI 181 I Sbjct: 445 SI 446 >AGc2451 [I] COG0439 Biotin carboxylase Length = 218 Score = 217 bits (553), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 0/179 (0%), Positives = 0/179 (0%), Gaps = 2/179 (1%) Query: 1 VNYSGAGTLEFLLGPDGQFYFMEMNTRIQVEHPVTEMVTGVDLIKEMISVAEGNPLSFTQ 60 Sbjct: 265 - 323 Query: 61 EDIKINGWSMECRINAEDPERNFMPSPGKIGFYLPPGGLGVRVDSAAYPGYTISPFYDSM 120 Sbjct: 324 - 382 Query: 121 IAKLIVWAPTRREAIAKMKRALSEFAVEGIHTTISFHQKLLEHPVFLDGNFDIKFLEEY 179 Sbjct: 383 441 >mll0203 [I] COG0439 Biotin carboxylase Length = 447 Score = 211 bits (536), Expect = 6e-55, Method: Compositional matrix adjust. Identities = 101/179 (56%), Positives = 130/179 (72%), Gaps = 2/179 (1%) Query: 1 VNYSGAGTLEFLLGPDGQFYFMEMNTRIQVEHPVTEMVTGVDLIKEMISVAEGNPLSFTQ 60 + YSGAGT+EFL +G+FYF+EMNTR+QVEHPVTE +TG+DL+ E I VA G LS Q Sbjct: 266 LGYSGAGTIEFLY-ENGEFYFIEMNTRLQVEHPVTEAITGIDLVHEQIRVASGGGLSVKQ 324 Query: 61 EDIKINGWSMECRINAEDPERNFMPSPGKIGFYLPPGGLGVRVDSAAYPGYTISPFYDSM 120 EDIK NG ++ECRINAEDP R F PSPG I + PGGLG+RVDS Y GY I P+YDS+ Sbjct: 325 EDIKFNGHAIECRINAEDP-RTFTPSPGTITHFHTPGGLGIRVDSGVYSGYKIPPYYDSL 383 Query: 121 IAKLIVWAPTRREAIAKMKRALSEFAVEGIHTTISFHQKLLEHPVFLDGNFDIKFLEEY 179 I KLIV R E + +++RAL EF V+GI TT+ + L+ + +G++DI +LE+Y Sbjct: 384 IGKLIVHGRNRVECMMRLRRALDEFVVDGIKTTLPLFRDLVGNADIANGDYDIHWLEKY 442 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.139 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,183,079 Number of extensions: 578076 Number of successful extensions: 896 Number of sequences better than 1.0e-05: 53 Number of HSP's gapped: 817 Number of HSP's successfully gapped: 53 Length of query: 181 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 81 Effective length of database: 26,845,031 Effective search space: 2174447511 Effective search space used: 2174447511 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 112 (47.8 bits)