BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0137.0003.p (301 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yitT [S] COG1284 Uncharacterized conserved protein 189 4e-48 BH2916 [S] COG1284 Uncharacterized conserved protein 169 3e-42 lin2365 [S] COG1284 Uncharacterized conserved protein 167 2e-41 SP2113 [S] COG1284 Uncharacterized conserved protein 148 9e-36 L22691 [S] COG1284 Uncharacterized conserved protein 142 9e-34 >BS_yitT [S] COG1284 Uncharacterized conserved protein Length = 280 Score = 189 bits (481), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 101/278 (36%), Positives = 162/278 (58%), Gaps = 6/278 (2%) Query: 28 VAKQVKEVAIIIFSAFLVASGLRLFLIPHQXXXXXXXXXXXXIGYLTN---PKYISL--Y 82 V Q K++ I+I A L A+GL LFLIP + + + P YIS Sbjct: 2 VLDQTKKLLIVIIGALLNAAGLNLFLIPADVYASGFTGVAQLLSSVVDQYAPFYISTGTL 61 Query: 83 YFAINLPILIWGFVAVGKKYIFYSLLSVVATTWFLTVIPLIKLTKDPILXXXXXXXXXXX 142 F +N+P+ I G++ VGK + YS+LSV TT F+ ++P L+ D +L Sbjct: 62 LFLLNIPVGILGWLKVGKSFTVYSILSVALTTLFMGILPETSLSHDILLNAVFGGVISAV 121 Query: 143 XXXXXLRAGGSSGGFDILGSIITRKRDIPMGTVLFVLDGLVILSLGFFKSWDSALYAMLC 202 L+ G S+GG DI+ ++ + +D P+GT F+L+G++IL+ G + W+ ALY ++ Sbjct: 122 GIGLTLKYGASTGGLDIVAMVLAKWKDKPVGTYFFILNGIIILTAGLLQGWEKALYTLVT 181 Query: 203 IFVKSRVVDMIHIRHIKLTCFIITKEREKMLNRLTC-LPHGITVVNAEGGYSHEGNTMLM 261 ++V +RV+D IH RH+KLT I+TK+ +++ + + GIT V A+G +++E M++ Sbjct: 182 LYVTTRVIDAIHTRHMKLTAMIVTKKADEIKEAIYGKMVRGITTVPAKGAFTNEQKEMMI 241 Query: 262 TVTTRYELAELRKTILETDPKSFVNVLETVEIVGRFRR 299 V TRYEL +L K + E DPK+F N+++T I G FR+ Sbjct: 242 IVITRYELYDLEKIVKEVDPKAFTNIVQTTGIFGFFRK 279 >BH2916 [S] COG1284 Uncharacterized conserved protein Length = 293 Score = 169 bits (429), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 93/274 (33%), Positives = 151/274 (55%), Gaps = 8/274 (2%) Query: 30 KQVKEVAIIIFSAFLVASGLRLFLIPHQXXXXXXXXXXXXIGYLTNPKYISLYYFAINLP 89 ++K++ +++ A +A+ L FLIP + L P + +N+P Sbjct: 23 NELKKIVVVVIGAVFLAASLNFFLIPADVFASGFTGVAQILATLL-PLSTGILLLLLNIP 81 Query: 90 ILIWGFVAVGKKYIFYSLLSVVATTWFLTVIPLIKLTKDPILXXXXXXXXXXXXXXXXLR 149 + I G++ +GK + YS LSV TT FL V P+ + D +L L+ Sbjct: 82 VAIIGWLKLGKAFTVYSFLSVALTTLFLEVFPVQAFSPDILLNAVFGGVVAAIGAGITLK 141 Query: 150 AGGSSGGFDILGSIITRKRDIPMGTVLFVLDGLVILSLGFFKSWDSALYAMLCIFVKSRV 209 G SSGG DI+ +++R D P+G F+L+GL++++ GF + ALY ++ ++V SR+ Sbjct: 142 VGASSGGLDIIALLLSRVSDRPVGIYFFLLNGLIVVASGFLFDAEKALYTLVTLYVTSRI 201 Query: 210 VDMIHIRHIKLTCFIITKE----REKMLNRLTCLPHGITVVNAEGGYSHEGNTMLMTVTT 265 +D IH RH+KLT I+TK+ R+ + R+T GIT + A+GGY + +LM V T Sbjct: 202 IDAIHTRHVKLTAMIVTKKPTELRKAIHERIT---RGITRMPAKGGYLEDDKEVLMIVLT 258 Query: 266 RYELAELRKTILETDPKSFVNVLETVEIVGRFRR 299 RYEL +LR + E DP++F N++ T + G FR+ Sbjct: 259 RYELYDLRHVVQEVDPEAFTNIVHTEGVYGLFRK 292 >lin2365 [S] COG1284 Uncharacterized conserved protein Length = 300 Score = 167 bits (423), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 100/272 (36%), Positives = 149/272 (54%), Gaps = 6/272 (2%) Query: 33 KEVAIIIFSAFLVASGLRLFLIPHQXXXXXXXXXXXX-IGYLTNPKYIS----LYYFAIN 87 K+ AI I +A L A G+ FLIP Q L + IS L +N Sbjct: 12 KKTAIAIIAALLNAIGMNFFLIPAQVYAAGLNGVAQLGSDMLRDTMNISISTGLLVLLLN 71 Query: 88 LPILIWGFVAVGKKYIFYSLLSVVATTWFLTVIPLIKLTKDPILXXXXXXXXXXXXXXXX 147 +P+ I G++ VGK + +S L+V ++FL VIP ++++ D +L Sbjct: 72 IPVAILGWLKVGKSFTVFSFLTVAFMSFFLIVIPEVQVSNDILLNAIFGALIASVGVGLA 131 Query: 148 LRAGGSSGGFDILGSIITRKRDIPMGTVLFVLDGLVILSLGFFKSWDSALYAMLCIFVKS 207 L+ G S+GG DIL IT K G +L+G++I+ GF W ALY ++ ++V+S Sbjct: 132 LKFGISTGGLDILAMYITIKTGRSFGKYFLLLNGIIIIVAGFAYDWTFALYTLISLYVQS 191 Query: 208 RVVDMIHIRHIKLTCFIITKEREKMLNRL-TCLPHGITVVNAEGGYSHEGNTMLMTVTTR 266 RV+D+IH RH KLT I+T+ E ++ + + GITVV+A GGYS + ML+ V TR Sbjct: 192 RVIDIIHTRHQKLTVMIMTQHSETVIKAIHENMVRGITVVDAMGGYSKQDVAMLIMVITR 251 Query: 267 YELAELRKTILETDPKSFVNVLETVEIVGRFR 298 YEL ++ + E DPK+F+NV+ET + G FR Sbjct: 252 YELYDITHIVGEFDPKAFINVMETSSVFGDFR 283 >SP2113 [S] COG1284 Uncharacterized conserved protein Length = 313 Score = 148 bits (374), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 84/263 (31%), Positives = 140/263 (53%), Gaps = 7/263 (2%) Query: 42 AFLVASGLRLFLIPHQXXXXXXXXXXXXIGYLTNPKY-----ISLYYFAINLPILIWGFV 96 FL A + F P I L+N + ISL ++AIN P+++ + Sbjct: 46 GFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVLAWY 105 Query: 97 AVGKKYIFYSLLSVVATTWFLTVIPLIKLTKDPILXXXXXXXXXXXXXXXXLRAGGSSGG 156 +G K+ ++ ++V +++F+ +P+ LT+DPI+ LR SSGG Sbjct: 106 QIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNNISSGG 165 Query: 157 FDILGSIITRKRDIPMGTVLFVLDGLVILSLGFFKSWDSALYAMLCIFVKSRVVDMIHIR 216 DI+ I +K +G++ F+++G ++L G W ALY+M+ IFV SRV D + + Sbjct: 166 TDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRVTDAVFTK 225 Query: 217 HIKLTCFIITKEREKMLNRLTCLPH-GITVV-NAEGGYSHEGNTMLMTVTTRYELAELRK 274 ++ I+T EK++ ++ H G T++ +AEG Y+HE +L+TV TR E E ++ Sbjct: 226 QKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVITRAEFNEFKQ 285 Query: 275 TILETDPKSFVNVLETVEIVGRF 297 + + DP SFV+V E V I+GRF Sbjct: 286 IMTQVDPSSFVSVSENVHILGRF 308 >L22691 [S] COG1284 Uncharacterized conserved protein Length = 311 Score = 142 bits (357), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 10/220 (4%), Positives = 27/220 (12%), Gaps = 8/220 (3%) Query: 86 INLPILIWGFVAVGKKYIFYSLLSVVATTWFLTVIPLIKLTKDPILXXXXXXXXXXXXXX 145 + + + + + + Sbjct: 62 AYMKLDVHDQETKIQDPKDNKKTINNPDHLKIYDSIKKVPNVKTVEFSSKEAQLKELTKA 121 Query: 146 XXLRAGGSSGGFDILGSIITRKRDIPMGTVLFVLDGLVILSLGFFKSWDSALYAMLCIFV 205 G + L + D P + + + + I Sbjct: 122 LGNNWNLFKGDANPLYDAYIVEADKPANVKSVTSKISKLSGIEKATYGGANTAKLFQIAQ 181 Query: 206 KSRVVDMIHIRHIKLTCFIITKERE----KMLNRLTCLPHGITVV-NAEGGYSHEGNTML 260 R + + R + +V G Sbjct: 182 GIRTWGTAAAILLIFVAVFLISNTIRITILSRQR---EIQIMRLVGAKNGYIRWPFFLEG 238 Query: 261 MTVTTRYELAELRKTILETDPKSFVNVLETVEIVGRFRRL 300 V + Sbjct: 239 AWVGLLGSIIPGLIVAWAYRLAFVSLTPSLQSQKLAMFAP 278 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.143 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,897,686 Number of extensions: 669046 Number of successful extensions: 1715 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 1658 Number of HSP's successfully gapped: 29 Length of query: 301 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 195 Effective length of database: 26,153,027 Effective search space: 5099840265 Effective search space used: 5099840265 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 115 (48.9 bits)