BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0137.0002.p (412 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_lysC [E] COG0527 Aspartokinases 416 e-116 lin1198 [E] COG0527 Aspartokinases 385 e-107 BH3096 [E] COG0527 Aspartokinases 383 e-106 TM0547_2 [E] COG0527 Aspartokinases 317 3e-86 TM1518 [E] COG0527 Aspartokinases 288 1e-77 >BS_lysC [E] COG0527 Aspartokinases Length = 408 Score = 416 bits (1068), Expect = e-116, Method: Compositional matrix adjust. Identities = 214/397 (53%), Positives = 287/397 (72%), Gaps = 3/397 (0%) Query: 2 KFGGSSVGDIERMKRVAGR-IADKQDEGHRCVVVVSAMGDTTDELIDQAKQLNGQPPARE 60 KFGG+SVG +E+++ A R IA+KQ +GH+ VVVVSAMG +TDEL+ AK ++ QP RE Sbjct: 7 KFGGTSVGSVEKIQNAANRAIAEKQ-KGHQVVVVVSAMGKSTDELVSLAKAISDQPSKRE 65 Query: 61 MDMLMTTGEQISVALLSIALSGIGRSAVSYTGWQAGFRTDETHGRARINEIDPRRVLESL 120 MDML+ TGEQ++++LLS+AL G AVSYTGWQAG RT+ HG ARI +ID + + L Sbjct: 66 MDMLLATGEQVTISLLSMALQEKGYDAVSYTGWQAGIRTEAIHGNARITDIDTSVLADQL 125 Query: 121 ERGQIVIVAGFQGMTVDGEITTLGRGGSDTTXXXXXXXXXXDVCEIYTDVDGIYSTDPRI 180 E+G+IVIVAGFQGMT D EITTLGRGGSDTT D C+IYTDV G+++TDPR Sbjct: 126 EKGKIVIVAGFQGMTEDCEITTLGRGGSDTTAVALAAALKADKCDIYTDVPGVFTTDPRY 185 Query: 181 VKTARKLNEISYDEMLELANLGAAVLHPRAVEYAKRYQVKLVVRSSFNHNEGTVVKEEAS 240 VK+ARKL ISYDEMLELANLGA VLHPRAVE+AK YQV L VRSS GT+++EE+S Sbjct: 186 VKSARKLEGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTETEAGTLIEEESS 245 Query: 241 MEQGVVVSGIAYDKNVARISILGVPDVPGVLAQVFGKLAEEGVDVDIIVQSGVQNEKADF 300 MEQ ++V GIA++ + R++I G+ L+ +F LA+ ++VDII+Q+ ++ K Sbjct: 246 MEQNLIVRGIAFEDQITRVTIYGLTSGLTTLSTIFTTLAKRNINVDIIIQTQAED-KTGI 304 Query: 301 SFTVALSELAHAKKVIEGLHSELPYREVTSEDNLVKVSIVGAGMVSHPGVAAQMFDVISK 360 SF+V + V+E L + ++ +E L KVSIVG+GMVS+PGVAA+MF V+++ Sbjct: 305 SFSVKTEDADQTVAVLEEYKDALEFEKIETESKLAKVSIVGSGMVSNPGVAAEMFAVLAQ 364 Query: 361 EGVSIKMVSTSEIKVSCVIESGNLQKIIQALHTAYNL 397 + + IKMVSTSEIKVS V+ ++ K +++LH A+ L Sbjct: 365 KNILIKMVSTSEIKVSTVVSENDMVKAVESLHDAFEL 401 >lin1198 [E] COG0527 Aspartokinases Length = 404 Score = 385 bits (990), Expect = e-107, Method: Compositional matrix adjust. Identities = 193/398 (48%), Positives = 273/398 (68%) Query: 1 MKFGGSSVGDIERMKRVAGRIADKQDEGHRCVVVVSAMGDTTDELIDQAKQLNGQPPARE 60 +KFGG+SV ++++ + A + ++ +G++ +VVVSAMG +TD+L+ A++++ P RE Sbjct: 6 LKFGGTSVSTVDKIGKTADQAIYEKKQGNKVIVVVSAMGKSTDKLVAMAEEISEAPDKRE 65 Query: 61 MDMLMTTGEQISVALLSIALSGIGRSAVSYTGWQAGFRTDETHGRARINEIDPRRVLESL 120 MDML++TGEQI++ALL++ L G A+SYTGWQAG T+ H ARI +ID R+ +L Sbjct: 66 MDMLLSTGEQITIALLAMTLKEKGHDAISYTGWQAGIETEAVHSNARIADIDSARIESAL 125 Query: 121 ERGQIVIVAGFQGMTVDGEITTLGRGGSDTTXXXXXXXXXXDVCEIYTDVDGIYSTDPRI 180 E GQIVIVAGFQG+T DGEITTLGRGGSDTT + C I TDV G+++TDPR Sbjct: 126 EAGQIVIVAGFQGITTDGEITTLGRGGSDTTAVAIASALKAEKCAICTDVVGVFTTDPRY 185 Query: 181 VKTARKLNEISYDEMLELANLGAAVLHPRAVEYAKRYQVKLVVRSSFNHNEGTVVKEEAS 240 VK A+KL +++YDEMLELANLGA VLHPR+VEYAK +++ L VR+S GT+++E+ + Sbjct: 186 VKKAQKLEQLTYDEMLELANLGAGVLHPRSVEYAKNFRIPLEVRASHELEPGTMIEEDLT 245 Query: 241 MEQGVVVSGIAYDKNVARISILGVPDVPGVLAQVFGKLAEEGVDVDIIVQSGVQNEKADF 300 ME VV GIA++ + R++I +++VF KLAE +DVDII+Q + Sbjct: 246 MENTKVVRGIAFEDQITRVTIHWKQKEAMRVSKVFTKLAENNIDVDIIIQGITGLGHGNL 305 Query: 301 SFTVALSELAHAKKVIEGLHSELPYREVTSEDNLVKVSIVGAGMVSHPGVAAQMFDVISK 360 SFT+ SEL V+E L ++ SE L KVSIVG+GMVS+PGVAA+MF+ +++ Sbjct: 306 SFTIKTSELLATLAVLEESKELLQIEKLESEQELAKVSIVGSGMVSNPGVAAKMFEALTE 365 Query: 361 EGVSIKMVSTSEIKVSCVIESGNLQKIIQALHTAYNLD 398 + IKM+STSEIKVS V+ + + + Q LH + LD Sbjct: 366 NNIPIKMISTSEIKVSTVVPARKMVEAAQVLHDQFELD 403 >BH3096 [E] COG0527 Aspartokinases Length = 412 Score = 383 bits (984), Expect = e-106, Method: Compositional matrix adjust. Identities = 198/402 (49%), Positives = 279/402 (69%), Gaps = 1/402 (0%) Query: 2 KFGGSSVGDIERMKRVAGRIADKQDEGHRCVVVVSAMGDTTDELIDQAKQLNGQPPAREM 61 K+GG+SVG ++R++ VA RI G + VVVVSAMG TTDEL+ A ++ +P REM Sbjct: 7 KYGGTSVGSVQRIEHVANRIIATAASGKKVVVVVSAMGKTTDELVGLASEITDEPRKREM 66 Query: 62 DMLMTTGEQISVALLSIALSGIGRSAVSYTGWQAGFRTDETHGRARINEIDPRRVLESLE 121 DML+ TGEQ+++ALL++AL G+ A S TGWQAG T+ HG ARI E++ R+ E L+ Sbjct: 67 DMLLATGEQVTIALLAMALHKRGQEATSLTGWQAGMITESHHGNARILEVERARMDELLD 126 Query: 122 RGQIVIVAGFQGMTVDGEITTLGRGGSDTTXXXXXXXXXXDVCEIYTDVDGIYSTDPRIV 181 + +VIVAGFQG+ GEI TLGRGGSDT+ + CEIYTDV G+++TDPR+V Sbjct: 127 KVHVVIVAGFQGIDQQGEIMTLGRGGSDTSAVAIAAALKAERCEIYTDVTGVFTTDPRVV 186 Query: 182 KTARKLNEISYDEMLELANLGAAVLHPRAVEYAKRYQVKLVVRSSFNHNEGTVVKEEASM 241 K ARKL ISYDEMLE+ANLGA+VLHPRAVE+AK Y++ L+V SS GT+++EE +M Sbjct: 187 KQARKLQTISYDEMLEMANLGASVLHPRAVEFAKNYEIPLLVASSMVDEPGTMIEEEGTM 246 Query: 242 EQGVVVSGIAYDKNVARISILGVPDVPGVLAQVFGKLAEEGVDVDIIVQSGVQNEKADFS 301 EQ ++V GIA++ +V ++++ G+P+ L +F L+ G++VDII+Q+ + A S Sbjct: 247 EQNLLVRGIAFENDVTKVTVHGLPNEIHTLPTLFAHLSAAGINVDIIIQNQTDSGMA-IS 305 Query: 302 FTVALSELAHAKKVIEGLHSELPYREVTSEDNLVKVSIVGAGMVSHPGVAAQMFDVISKE 361 F++ S L +E L EL ++ + E+ L KVSIVG+GMVS+PGVAA+MF++++ Sbjct: 306 FSITQSSLKETLARLEELKGELSFQGIDYEEELAKVSIVGSGMVSNPGVAAKMFEILAAN 365 Query: 362 GVSIKMVSTSEIKVSCVIESGNLQKIIQALHTAYNLDTEEQA 403 ++IKMVSTSEIKVS VI+ + ++ LH + L EE A Sbjct: 366 DIAIKMVSTSEIKVSAVIDEELMVLAVEMLHEQFKLHEEEPA 407 >TM0547_2 [E] COG0527 Aspartokinases Length = 402 Score = 317 bits (812), Expect = 3e-86, Method: Compositional matrix adjust. Identities = 163/400 (40%), Positives = 261/400 (65%), Gaps = 3/400 (0%) Query: 1 MKFGGSSVGDIERMKRVAGRIADKQDEGHRCVVVVSAMGDTTDELIDQAKQLNGQPPARE 60 MKFGG+++ D+E++++VA +I ++ G + VVV+SAMGDTTD LI+ AK ++ P RE Sbjct: 6 MKFGGAAISDVEKLEKVAEKIIKRKKSGVKPVVVLSAMGDTTDHLIELAKTIDENPDPRE 65 Query: 61 MDMLMTTGEQISVALLSIALSGIGRSAVSYTGWQAGFRTDETHGRARINEIDPRRVLESL 120 +D+L++TGE SVAL+SIAL G A+S+TG Q TD+ +G ARI +I+ + L Sbjct: 66 LDLLLSTGEIQSVALMSIALRKRGYKAISFTGNQLKIITDKRYGSARIIDINTDIISRYL 125 Query: 121 ERGQIVIVAGFQGMTVDGEITTLGRGGSDTTXXXXXXXXXXDVCEIYTDVDGIYSTDPRI 180 ++ I +VAGFQG+T G+ITTLGRGGSD T D+CE+Y DVDG+Y+ DPRI Sbjct: 126 KQDFIPVVAGFQGITETGDITTLGRGGSDLTAIALAYSLGADLCELYKDVDGVYTADPRI 185 Query: 181 VKTARKLNEISYDEMLELANLGAAVLHPRAVEYAKRYQVKLVVRSSFNHNEGTVVKEEAS 240 VK AR + E+S++EM+EL+ GA VL RA E+A++Y VK++++++ GT++ E Sbjct: 186 VKDARVIKELSWEEMIELSRHGAQVLQARAAEFARKYGVKVLIKNAHKETRGTLIWEGTK 245 Query: 241 MEQGVVVSGIAYDKNVARISILGVPDVPGVLAQVFGKLAEEGVDVDIIVQSGVQNEKADF 300 +E +V + ++ +A++ + VPD PGV A++ L++ GV++D+I+Q E Sbjct: 246 VENP-IVRAVTFEDGMAKVVLKDVPDKPGVAARIMRTLSQMGVNIDMIIQGMKSGEYNTV 304 Query: 301 SFTVALSELAHAKKVIEGLHSELPYREVTSEDNLVKVSIVGAGMVSHPGVAAQMFDVISK 360 +F V S+L K I+ L + +E+ E L KVSIVG + S P ++A +F+ ++ Sbjct: 305 AFIVPESQL--GKLDIDLLKTRSEAKEIIIEKGLAKVSIVGVNLTSTPEISATLFETLAN 362 Query: 361 EGVSIKMVSTSEIKVSCVIESGNLQKIIQALHTAYNLDTE 400 EG++I M+S S ++S +I+ ++ ++A+H+ + LD E Sbjct: 363 EGINIDMISASSSRISVIIDGKYVEDAVKAIHSRFELDRE 402 >TM1518 [E] COG0527 Aspartokinases Length = 401 Score = 288 bits (737), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 169/404 (41%), Positives = 242/404 (59%), Gaps = 17/404 (4%) Query: 2 KFGGSSVGDIERMKRVAGRIADKQDEGHRCVVVVSAMGDTTDELIDQAKQLNGQPPAREM 61 K+GGSSV ER+K VA RI K DEG++ VVVVSAMG TTD LI AK+++ +P +RE+ Sbjct: 7 KYGGSSVATPERIKNVAQRIKKKVDEGYKVVVVVSAMGKTTDNLIKLAKEISPRPDSREL 66 Query: 62 DMLMTTGEQISVALLSIALSGIGRSAVSYTGWQAGFRTDETHGRARINEIDPRRVLESLE 121 DML+ TGEQ+S ALLS+AL +G A S +Q +T H ARI +ID + E+L+ Sbjct: 67 DMLLATGEQVSAALLSMALKDLGVKAKSLNAFQVKIKTTPHHTSARIVDIDDSVIRENLK 126 Query: 122 RGQIVIVAGFQGMTVDGEITTLGRGGSDTTXXXXXXXXXXDVCEIYTDVDGIYSTDPRIV 181 +++V GFQG+ G++TTLGRGGSDT+ CEIY+DVDGIY+ DPR+ Sbjct: 127 DYDVLVVTGFQGVNEHGDLTTLGRGGSDTSAVALAAKLRVP-CEIYSDVDGIYTCDPRVH 185 Query: 182 KTARKLNEISYDEMLELANLGAAVLHPRAVEYAKRYQVKLVVRSSFNHNEGTVVKEE-AS 240 A+KL I+YDE LEL LGA VLH R+VE AK+Y + + SSF EGT+V E Sbjct: 186 PRAKKLAYITYDEALELTALGAKVLHSRSVEIAKKYGIPIYCASSFTEEEGTMVVERLPE 245 Query: 241 MEQGVVVSGIAYDKNVARISILGVPDVPGVLAQVFGKLAEEGVDVDIIVQSGVQNEKADF 300 + VV+G ++SI +P + +F ++ + ++VD+I N K Sbjct: 246 WLEEPVVTGATISHGQIKVSISFLPKDVKYITAIFEEVGKRALNVDMISLVP-SNGKVFL 304 Query: 301 SFTVA------LSE-LAHAKKVIEGLHSELPYREVTSEDNLVKVSIVGAGMVSHPGVAAQ 353 SFT+ L E L A + +EG S T E K+SIVG GM + PGVAA+ Sbjct: 305 SFTILEDHKEDLDEALKEALRDVEGWKS-------TYEGGFAKLSIVGVGMRTSPGVAAR 357 Query: 354 MFDVISKEGVSIKMVSTSEIKVSCVIESGNLQKIIQALHTAYNL 397 F+ + + GV+ ++V+TSEIK+SC++ ++ ++++ + L Sbjct: 358 FFEALERAGVTPELVTTSEIKISCLVPEEKAEEALKSVIEEFEL 401 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.133 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,119,778 Number of extensions: 1080474 Number of successful extensions: 2809 Number of sequences better than 1.0e-05: 51 Number of HSP's gapped: 2711 Number of HSP's successfully gapped: 52 Length of query: 412 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 303 Effective length of database: 25,807,025 Effective search space: 7819528575 Effective search space used: 7819528575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)