BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0135.0008.p (324 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0754 [T] COG0642 Signal transduction histidine kinase 199 7e-51 CAC0372 [T] COG0642 Signal transduction histidine kinase 171 2e-42 lin1852 [T] COG0642 Signal transduction histidine kinase 152 9e-37 CAC0225 [T] COG0642 Signal transduction histidine kinase 140 2e-33 ML0132 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 134 3e-31 >BH0754 [T] COG0642 Signal transduction histidine kinase Length = 353 Score = 199 bits (505), Expect = 7e-51, Method: Compositional matrix adjust. Identities = 104/240 (43%), Positives = 147/240 (61%), Gaps = 10/240 (4%) Query: 92 VLAHQFRLYQNDLHSYRHKLEEHIHFINQWVHGMKTPLSVIHLIIQDKDGPPFTAIGDEL 151 + F Y LH+ + + HF N+W H MKTPLSVI LI+Q++D + D++ Sbjct: 102 LFGQAFEDYSTRLHTAQEQHSRSYHFANRWTHQMKTPLSVIRLILQEQDEWSKEVLVDQI 161 Query: 152 D----RLKKGLDTVLYTARLDTFEHDFYVERLELAAIVRGVTSEQKRLFIRKRVFPAIRV 207 + +L+ GL +L T RL FE DF +ER+ L +++R V +++ FIR R++P + Sbjct: 162 EEEVGKLEDGLHMMLSTVRLQKFELDFSIERIALLSVIRSVLHGKRKTFIRYRIYPRLIS 221 Query: 208 E-NGLFITSDEKWLSFVLTQLITNALRYTV----EEGRFVHFHGYAGEQGRPVLEVRDEG 262 E +F +D+KWL FVL QLI NAL+Y+ E +++ + G V ++DEG Sbjct: 222 EAEEVFAATDKKWLIFVLEQLIQNALKYSSTLAREPSKYITVRVEETDNGVTV-SIQDEG 280 Query: 263 VGIPPGDLPRVFDAYFTGVNGRSFQESTGMGLYLVKQICGKLGHQVEISSEVGQGTAVRI 322 +GI DLPRVFD +FTG NGR QESTGMGLYLVKQIC +LGH + I S V +GT V + Sbjct: 281 IGIVAEDLPRVFDPFFTGENGRKLQESTGMGLYLVKQICDQLGHSITIESTVNEGTTVTL 340 >CAC0372 [T] COG0642 Signal transduction histidine kinase Length = 334 Score = 171 bits (432), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 82/215 (38%), Positives = 134/215 (62%), Gaps = 7/215 (3%) Query: 116 HFINQWVHGMKTPLSVIHLIIQDK----DGPPFTAIGDELDRLKKGLDTVLYTARLDTFE 171 +FI+QW+H MKTP+SVI LI+Q + G I +E ++K G+D VL RLD F Sbjct: 117 YFISQWIHNMKTPVSVIDLIVQREKEKLSGDVIKNIEEENYKIKNGMDQVLNIIRLDEFS 176 Query: 172 HDFYVERLELAAIVRGVTSEQKRLFIRKRVFPAIR--VENGLFITSDEKWLSFVLTQLIT 229 D+ E +++ +V+ + +K FI FP + V+ L +T D+KW +F++ Q+I+ Sbjct: 177 RDYEPEIVDIVELVKRTINLKKNQFIYGNAFPKVEFHVDKALVLT-DKKWSTFIIDQIIS 235 Query: 230 NALRYTVEEGRFVHFHGYAGEQGRPVLEVRDEGVGIPPGDLPRVFDAYFTGVNGRSFQES 289 N+++Y+ +E + + + L ++D GVGIP DL R+F+ +FTG NGR+F+ S Sbjct: 236 NSIKYSKKEEKGYVYFNIVQSNNKTHLVIKDNGVGIPKYDLKRIFEPFFTGENGRNFENS 295 Query: 290 TGMGLYLVKQICGKLGHQVEISSEVGQGTAVRIIF 324 TG+GLY+ K+I LGH++ + SE+ +GT V+I + Sbjct: 296 TGIGLYICKKIADNLGHEISVESEINKGTIVKITY 330 >lin1852 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 152 bits (383), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 0/222 (0%), Positives = 0/222 (0%), Gaps = 8/222 (3%) Query: 108 RHKLEEHIHFINQWVHGMKTPLSVIHLIIQDKD----GPPFTAIGDELDRLKKGLDTVLY 163 + + + D P+ + + Sbjct: 116 EVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSPWMVMQATNREARKRL 175 Query: 164 TARLDTFEHDFYVERLELAAIVRGVTSEQKRLFIRKRVFPAIRVEN-GLFITSDEKWLSF 222 Sbjct: 176 -- 233 Query: 223 VLTQLITNALRYTVEEGRFVHFHGYAGEQGRPVLEVRDEGVGIPPGDLPRVFDAYFTGVN 282 Sbjct: 234 - 292 Query: 283 GRSFQESTGMGLYLVKQICGKLGHQVEISSEVGQGTAVRIIF 324 Sbjct: 293 334 >CAC0225 [T] COG0642 Signal transduction histidine kinase Length = 339 Score = 140 bits (354), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 72/252 (28%), Positives = 136/252 (53%), Gaps = 11/252 (4%) Query: 79 PAQSSPLA---ESLRGVLAHQFRLYQNDLHSYRHKLEEHIHFINQWVHGMKTPLSVIHLI 135 P PL E ++ ++ Y + S + E++ F+ W+H +KTP++ L+ Sbjct: 81 PILPEPLEYKDEVYISIIEDLYKDYNERIVSLEKEFEDNNEFMTAWIHEIKTPIATAKLL 140 Query: 136 IQ--DKDGPPFTAIGDELDRLKKGLDTVLYTARLDTFEHDFYVERLELAAIVRGVTSEQK 193 ++ + D F +E++++ ++ VLY +R D F D+ + + ++ +++ Sbjct: 141 LESGEIDSQLFM---EEIEKIDDYVEKVLYYSRSDNFSKDYIISEVNISKLIKECIKSHS 197 Query: 194 RLFIRKRVFPAIRVENGLFITSDEKWLSFVLTQLITNALRYTVEEGRFVHFHGYAGEQGR 253 ++FI+K + + ++ + +D+KW F+L Q+I+NAL+YT + G + + + + Sbjct: 198 KIFIKKHIKLKLEIDERFNVETDKKWFLFILNQIISNALKYTNDMGNII-IKAFEDDNEK 256 Query: 254 PVLEVRDEGVGIPPGDLPRVFDAYFTGVNGRS-FQESTGMGLYLVKQICGKLGHQVEISS 312 V+ + D G+GI DL R+F FTG NGR ++TGMGLYL ++ KLGH + I S Sbjct: 257 VVI-IEDNGIGIKKEDLDRIFSKSFTGYNGRKENSKATGMGLYLSNKLAEKLGHNITIES 315 Query: 313 EVGQGTAVRIIF 324 + +GT + I F Sbjct: 316 KYNEGTKLYIHF 327 >ML0132 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Length = 211 Score = 134 bits (336), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 0/205 (0%), Positives = 0/205 (0%), Gaps = 11/205 (5%) Query: 118 INQWVHGMKTPLSVIHLIIQDKDGPPFTAIGDELDRLKKGLDTVLYTARLDTFEHDFYVE 177 I H + P L G L+ + T + Sbjct: 95 ILLEQHNGEVPEDRAALEALPGVGRK--TANVVLNTAFGWPTIAVDTHIFRVCNRTQFAP 152 Query: 178 RLELAAIVRGVTSEQKRLFIRKRVFPAIRVENGLFITSDEKWLSFVLTQLITNALRYTVE 237 + + + F + +G + K + V+ Sbjct: 153 GKNVEQVEEKLLKVVPAEFKVDCH--HWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVD 210 Query: 238 EGRFVHFHGYAGEQGRPVLEVRDEGVGIPPGDLPRVFDAYFTGVNGRSFQESTGMGLYLV 297 Sbjct: 211 ------- 263 Query: 298 KQICGKLGHQVEISSEVGQGTAVRI 322 Sbjct: 264 288 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.142 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,853,582 Number of extensions: 1067355 Number of successful extensions: 4363 Number of sequences better than 1.0e-05: 98 Number of HSP's gapped: 4352 Number of HSP's successfully gapped: 98 Length of query: 324 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 217 Effective length of database: 26,037,693 Effective search space: 5650179381 Effective search space used: 5650179381 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 116 (49.3 bits)