BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0135.0005.p (360 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2614 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzy... 296 3e-80 BS_yqjM [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzy... 295 6e-80 CAC3229 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzy... 281 2e-75 all0827 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzy... 269 4e-72 PA3723 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzym... 267 2e-71 >lin2614 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzyme family Length = 346 Score = 296 bits (759), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 26/339 (7%), Positives = 49/339 (14%), Gaps = 9/339 (2%) Query: 16 MKDLFTPYALQNLKLKNRVVMPPMCQYSVTDKDGIATDWHYNHYVSRAVGGTGLIIMEMT 75 M+ P++ + M Q S D V + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 76 DVDPDGRITDYDLGIWSDEHIPALARIVEACHSYGAKVGIQIAHAGRKAEDAQLPVAPSA 135 L +A V K RK L + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 136 IPFSAEYKTPRELTTEEVKAMVQKFQAGVERAVQAGFDVIELHGAHGYLIHQFHSPLTNK 195 + + + + A A + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 196 RTDEYG---KDLTLFGREVIQAAKKVMPEGMPLIMRISAQEYVEGGYGIRESLEIGRAYK 252 T L + L Y E L Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNP-YS-LLNRSFEVGLAEVSQYEGVELLAYSCLGF 238 Query: 253 EAGADIFHVSXXXXXXXXXXXKPGTHAAYQIPLARAFKQELNVPVIAVGRLDEAVLANAV 312 + + + K IA + Sbjct: 239 GTLTGKYLNGAKPAGARNTLFSRF----TRYSGEQTQKAVAAYVDIARRHGLDPAQMALA 294 Query: 313 IGNDEADLVAVGRGMLRNPYWTLEAGVQLGKDAGLPKQY 351 + + + G + + Sbjct: 295 FVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAE 333 >BS_yqjM [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzyme family Length = 346 Score = 295 bits (756), Expect = 6e-80, Method: Compositional matrix adjust. Identities = 22/344 (6%), Positives = 64/344 (18%), Gaps = 9/344 (2%) Query: 15 MMKDLFTPYALQNLKLKNRVVMPPMCQYSVTDKDGIATDWHYNHYVSRAVGGTGLIIMEM 74 M +L+ L + + + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 75 TDVDPDGRITDYDLGIWSDEHIPALARIVEACHSYGAKVGIQIAHAGRKAEDAQLPVAPS 134 T + + ++ S E + +++ + + A + +A Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 135 AIPFSAEYKTPRELTTEEVKAMVQKFQAGVERAVQAGFDVIELHGAHGYLIHQFHSPLTN 194 + + + D ++ + + ++N Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 195 KRTDEYG---KDLTLFGREVIQAAKKVMPEGMPLIMRISAQEYVEGGYGIRESLEIGRAY 251 + I + V G+ + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPY-SL-LNRSFEVGLAEVSQYEGVELLAYSCLGF 238 Query: 252 KEAGADIFHVSXXXXXXXXXXXKPGTHAAYQIPLARAFKQELNVPVIAVGRLDEAVLANA 311 + + + V + LD A +A A Sbjct: 239 GTLTGKYLNGAKPAGARNTLFSRFT----RYSGEQTQKAVAAYVDIARRHGLDPAQMALA 294 Query: 312 VIGNDEADLVAVGRGMLRNPYWTLEAGVQLGKDAGLPKQYELGF 355 + + + T + L + + E Sbjct: 295 FVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVH 338 >CAC3229 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzyme family Length = 346 Score = 281 bits (718), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 20/343 (5%), Positives = 57/343 (16%), Gaps = 13/343 (3%) Query: 19 LFTPYALQNLKLKNRVVMPPMCQYSVTDKDGIATDWHYNHYVSRAVGGTGLIIMEMTDVD 78 + + + ++ D A + + + + Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQN-SEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 79 PDGRITDYDLGIWSDEHIPALARIVEACHSYGAKVGIQIAHAGRKAEDAQ-LPVAPSAIP 137 T + L + ++ + Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 138 FSAEYKTPRELTTEEVKAMVQKFQAGVERAVQAGFDVIELHGAHGYLIHQFHSPLTNKRT 197 + + + K + A + L Y + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 198 DEYG---KDLTLFGREVIQAAKKVMPEGMPLIMRISAQEYVEGGYGI--RESLEIGRAYK 252 +G ++ + P + Y + Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAE-VSQYEGVELLAYSCLGFGT 240 Query: 253 EAGADIFHVSXXXXXXXXXXXKPGTHAAYQIPLARAFKQELNVPVIAVGRLDEAVLANAV 312 + + + V + LD A +A A Sbjct: 241 LT-GKYLNGAKPAGARNTLFSRFT----RYSGEQTQKAVAAYVDIARRHGLDPAQMALAF 295 Query: 313 IGNDEADLVAVGRGMLRNPYWTLEAGVQLGKDAGLPKQYELGF 355 + + + T + L + + E Sbjct: 296 VRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVH 338 >all0827 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzyme family Length = 346 Score = 269 bits (688), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 0/360 (0%), Positives = 0/360 (0%), Gaps = 26/360 (7%) Query: 16 MKDLFTPYALQNLKLKNRVVMPPMCQYSVTDKDGIATDWHYNHYVSRAVGGTGLIIMEMT 75 M+ P++ + M Q S D A + + + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEAD--AHAQLDYAVAQGINLIDVAEMYPVPPR 58 Query: 76 DVDPDGRITDYDLGIWSDEHIPALARIVEACHSYGAKVGIQIAHAGRKAEDAQLP----- 130 T + L + ++ + Sbjct: 59 PETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSL 118 Query: 131 -----------VAPSAIPFSAEYKTPRELTTEEVKAMVQKFQAGVERAVQAGFDVIELHG 179 + + T+ A+ Q + + Sbjct: 119 KRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGV 178 Query: 180 AHGYLIHQFHSPLTNKRTDEYG---KDLTLFGREVIQAAKKVMPEGMPLIMRISAQEYVE 236 ++ + D + ++ Sbjct: 179 SNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGF 238 Query: 237 GGYGIRESLEIGRAYKEAGADIFHVSXXXXX-XXXXXXKPGTHAAYQIPLARAFKQELNV 295 G + A Q Sbjct: 239 GTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVD----IARRHGLDPAQMALA 294 Query: 296 PVIAVGRLDEAVLANAVIGNDEADLVAVGRGMLRNPYWTLEAGVQLGKDAGLPKQYELGF 355 Sbjct: 295 354 >PA3723 [C] COG1902 NADH:flavin oxidoreductases, Old Yellow Enzyme family Length = 346 Score = 267 bits (683), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 0/357 (0%), Positives = 0/357 (0%), Gaps = 27/357 (7%) Query: 16 MKDLFTPYALQNLKLKNRVVMPPMCQYSVTDKDGIATDWHYNHYVSRAVGGTGLIIMEMT 75 M+ P++ + M Q S D A + + + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSE--ADAHAQLDYAVAQGINLIDVAEMYPVPPR 58 Query: 76 DVDPDGRITDYDLGIWSDEHIPALARIVEACHSYGAKVGIQIAHAGRKA----------- 124 T + L + Sbjct: 59 PETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSL 118 Query: 125 -----EDAQLPVAPSAIPFSAEYKTPRELTTEEVKAMVQKFQAGVERAVQAGFDVIELHG 179 + L + + T+ A+ Q + + Sbjct: 119 KRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGV 178 Query: 180 AHGYLIHQFHSPLTNKRTDEYGKDLTLFGREVIQ---AAKKVMPEGMPLIMRISAQEYVE 236 ++ + D ++ + ++ Sbjct: 179 SNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGF 238 Query: 237 GGYGIRESLEIGRAYKEAGADIFHVSX-XXXXXXXXXXKPGTHAAYQIPLARAFKQELNV 295 G + A Q Sbjct: 239 GTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVD----IARRHGLDPAQMALA 294 Query: 296 PVIAVGRLDEAVLANAVIGNDEADLVAVGRGMLRNPYWTLEAGVQL-GKDAGLPKQY 351 V + +L + + ++ ++ + + +EA Q+ Sbjct: 295 FVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVY 351 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,980,971 Number of extensions: 1069549 Number of successful extensions: 2517 Number of sequences better than 1.0e-05: 82 Number of HSP's gapped: 2262 Number of HSP's successfully gapped: 82 Length of query: 360 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 252 Effective length of database: 25,922,359 Effective search space: 6532434468 Effective search space used: 6532434468 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)