BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0135.0002.p (207 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value L119032 [S] COG0586 Uncharacterized membrane-associated protein 211 9e-55 lin1984 [S] COG0586 Uncharacterized membrane-associated protein 204 7e-53 DR2612 [S] COG0586 Uncharacterized membrane-associated protein 153 2e-37 slr0509 [S] COG0586 Uncharacterized membrane-associated protein 150 1e-36 all5093 [S] COG0586 Uncharacterized membrane-associated protein 147 1e-35 >L119032 [S] COG0586 Uncharacterized membrane-associated protein Length = 214 Score = 211 bits (536), Expect = 9e-55, Method: Compositional matrix adjust. Identities = 104/179 (58%), Positives = 135/179 (75%), Gaps = 1/179 (0%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 M+ I +M QFGY G+ ++ +ENIFPPIPSEVIL FGGFMTT S L I G++IA+T G Sbjct: 1 MQEIIIQVMNQFGYFGVAFLIMIENIFPPIPSEVILTFGGFMTTYSELGIIGMIIAATIG 60 Query: 61 SLLGAAILYWIGRLLEVSRMERIVD-RYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMV 119 S+LGA ILY++GRLL V R+E++V R GK +R+K DI KA+ WF K GY T+ FCR + Sbjct: 61 SVLGALILYFVGRLLSVERLEKLVSGRLGKVLRLKPEDITKAEKWFLKRGYATIFFCRFI 120 Query: 120 PLVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYSS 178 PL+RSLISIPAG + MK F++ TT+GTL WN +L+ LGAALG++WE I G + +YSS Sbjct: 121 PLIRSLISIPAGSAKMKLPSFLILTTLGTLIWNIVLVCLGAALGDNWEMIAGILDSYSS 179 >lin1984 [S] COG0586 Uncharacterized membrane-associated protein Length = 207 Score = 204 bits (519), Expect = 7e-53, Method: Compositional matrix adjust. Identities = 97/178 (54%), Positives = 133/178 (74%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 M+TWIT IM FGY GIFL++ +EN+FPPIPSE+IL FGGFMTT S L + V+I +T G Sbjct: 1 METWITSIMADFGYIGIFLLIMIENLFPPIPSEIILTFGGFMTTVSSLNVVMVIIVATLG 60 Query: 61 SLLGAAILYWIGRLLEVSRMERIVDRYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMVP 120 S++GA +LY + R+ IV +YG+ +R+K+SDI +A+++F KYG W V CRM+P Sbjct: 61 SVVGAILLYKVASYFGKERLTHIVLKYGRVLRLKESDIERAESFFLKYGSWAVFLCRMIP 120 Query: 121 LVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYSS 178 L+RSLISIPAGM+ MK F++ TT G+L WNT+LI LGA LGESW +I ++ ++S+ Sbjct: 121 LIRSLISIPAGMTKMKMSRFLILTTAGSLLWNTVLIGLGALLGESWGEIVVFMDSFST 178 >DR2612 [S] COG0586 Uncharacterized membrane-associated protein Length = 205 Score = 153 bits (386), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 77/177 (43%), Positives = 115/177 (64%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 M WI ++M+ GY GI L++ LEN+FPPIPSE+I+P GF +++ V++ T G Sbjct: 1 MTDWIQNLMDSMGYLGILLLMVLENVFPPIPSELIMPSAGFAAARGDMSLGMVVLMGTLG 60 Query: 61 SLLGAAILYWIGRLLEVSRMERIVDRYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMVP 120 S++G LY+IGR ++ D++GKW+ + DI+KADAWFD+YG VLF RMVP Sbjct: 61 SVIGTLPLYYIGRAFGEDKLVAWADKHGKWLTLSGKDIKKADAWFDRYGAGAVLFGRMVP 120 Query: 121 LVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYS 177 +RSL+S+PAGMS M F++++ IG+ W+ L G ALGE+++ + Y+ S Sbjct: 121 GIRSLLSLPAGMSEMPLPKFLIYSAIGSALWSAALAYAGYALGENYDRVEQYVAPVS 177 >slr0509 [S] COG0586 Uncharacterized membrane-associated protein Length = 205 Score = 150 bits (379), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 72/178 (40%), Positives = 110/178 (61%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 + WI +M Q GY GI L++ LEN+FPPIPSE+I+P GF L + ++A G Sbjct: 5 ITEWIPQVMNQLGYGGISLLMFLENLFPPIPSELIMPLAGFAVAQGKLELFPAIVAGIIG 64 Query: 61 SLLGAAILYWIGRLLEVSRMERIVDRYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMVP 120 ++LGA Y+IG+ + R+E++ DRYGKWI + DI K++ WF +YG+ +V F R+VP Sbjct: 65 TILGAYPWYYIGKWVSEERLEQLADRYGKWIGLDAKDIHKSNVWFGRYGHQSVFFGRLVP 124 Query: 121 LVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYSS 178 +R+++S+PAG++ M F L++ G W T L G LG+ +E + Y+G S Sbjct: 125 GIRTIVSLPAGVNAMGLISFTLYSLGGISLWVTFLASAGYKLGDHYELVEQYLGPVSK 182 >all5093 [S] COG0586 Uncharacterized membrane-associated protein Length = 205 Score = 147 bits (371), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 77/176 (43%), Positives = 105/176 (59%), Gaps = 2/176 (1%) Query: 4 WITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSG--LTIPGVLIASTAGS 61 WI +I+E GY GI L++ +EN+FPPIPSE+I+P G+ G L I GV IA GS Sbjct: 5 WIKNIIESLGYWGIGLLMFVENLFPPIPSELIMPLAGYTANLPGSRLNIIGVFIAGLLGS 64 Query: 62 LLGAAILYWIGRLLEVSRMERIVDRYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMVPL 121 +GA I Y+ G+ L R+ + D+YGKWI + DI KA WFD G V R++P Sbjct: 65 TIGALIWYYPGKFLGEQRLRYLADKYGKWISVSSKDITKAKRWFDSQGNKAVFMGRLIPG 124 Query: 122 VRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYS 177 VR+LIS+PAG+S M F+ +TT+G+ W LL G LG +E + Y+ S Sbjct: 125 VRTLISVPAGVSNMPLLPFLFYTTLGSAAWVGLLTYSGYLLGSQYELVDKYLAPVS 180 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.143 0.455 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,525,675 Number of extensions: 518925 Number of successful extensions: 1829 Number of sequences better than 1.0e-05: 64 Number of HSP's gapped: 1816 Number of HSP's successfully gapped: 64 Length of query: 207 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 105 Effective length of database: 26,614,363 Effective search space: 2794508115 Effective search space used: 2794508115 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 113 (48.1 bits)