BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0035.p (298 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1742 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneurami... 142 6e-34 BS_dapA [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuram... 134 2e-31 AGl1788 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuram... 124 1e-28 BMEI1301 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneura... 123 4e-28 AGc1883 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuram... 123 4e-28 >BH1742 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 295 Score = 142 bits (359), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 85/275 (30%), Positives = 140/275 (50%), Gaps = 2/275 (0%) Query: 13 IYVLAITPMMDTQAFDPSALRRNIDHYIASGAHGIIVGGTYAEYPSLTSDERKQLFTXXX 72 I +TP + + A + ++ IA+G+ ++VGGT E P+LT+DE+ LF Sbjct: 6 IVTAMVTPFNEQDQVNLEATCQLVNELIANGSDALVVGGTTGESPTLTTDEKMALFHTVV 65 Query: 73 XXXXGRVPLLCCTAASGTDEASELAHAAKLAGADGVMVTPPYVSEVRPQDILYHFQLLNE 132 GRVP++ T ++ T + EL+ + G DG+M+ PY ++ + + HF+ ++E Sbjct: 66 EAAGGRVPVIAGTGSNNTRASIELSKKVEDLGVDGIMLVTPYYNKPSQEGLFQHFKAISE 125 Query: 133 SAELPILIYN-SASIGVHLSPEEIAGLAKLDNVAGVKQGATDLHSLVRTVAYAGNDISVM 191 S LPI++YN GV+L E LA+L N+ +K+ + +L + + ++ D SV Sbjct: 126 STSLPIMLYNIPGRSGVNLDAETTLRLAELRNIVSIKEASGNLDQIAEIIEHSPADFSVY 185 Query: 192 CGSDGLILGGLASGLPGCTSTNANFMTSEFVALYNEFQQGENARALERYYRWQPIRDLAR 251 G D L L L+ G G S ++ + +E + F G+ RA + + P+ Sbjct: 186 SGDDSLTLPLLSIGGTGVVSVASHIIGNEMQEMVQLFLSGQVERAAAIHRKLLPLMKTLF 245 Query: 252 KYGQPAMVKAALDIIGLPSGPVRAPFRTL-GEDAR 285 P VKAAL++ GLP G VR P L E+ R Sbjct: 246 MTPNPTAVKAALELKGLPVGHVRLPLVPLTAEEQR 280 >BS_dapA [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 290 Score = 134 bits (336), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 83/279 (29%), Positives = 136/279 (48%), Gaps = 2/279 (0%) Query: 18 ITPMMDTQAFDPSALRRNIDHYIASGAHGIIVGGTYAEYPSLTSDERKQLFTXXXXXXXG 77 ITP + D L ID+ + +G ++V GT E P+L+++E+ LF G Sbjct: 11 ITPFDNKGNVDFQKLSTLIDYLLKNGTDSLVVAGTTGESPTLSTEEKIALFEYTVKEVNG 70 Query: 78 RVPLLCCTAASGTDEASELAHAAKLAGADGVMVTPPYVSEVRPQDILYHFQLLNESAELP 137 RVP++ T ++ T ++ +L A+ AG D VM+ PY ++ + + HF+ + LP Sbjct: 71 RVPVIAGTGSNNTKDSIKLTKKAEEAGVDAVMLVTPYYNKPSQEGMYQHFKAIAAETSLP 130 Query: 138 ILIYNSASIGV-HLSPEEIAGL-AKLDNVAGVKQGATDLHSLVRTVAYAGNDISVMCGSD 195 +++YN V L+PE L A + NV +K+ + DL ++ + +A D V G D Sbjct: 131 VMLYNVPGRTVASLAPETTIRLAADIPNVVAIKEASGDLEAITKIIAETPEDFYVYSGDD 190 Query: 196 GLILGGLASGLPGCTSTNANFMTSEFVALYNEFQQGENARALERYYRWQPIRDLARKYGQ 255 L L L+ G G S ++ ++ + + G+ A A + + PI K Sbjct: 191 ALTLPILSVGGRGVVSVASHIAGTDMQQMIKNYTNGQTANAALIHQKLLPIMKELFKAPN 250 Query: 256 PAMVKAALDIIGLPSGPVRAPFRTLGEDARADIARTLQQ 294 PA VK AL + GL G VR P L ED R ++ T+ + Sbjct: 251 PAPVKTALQLRGLDVGSVRLPLVPLTEDERLSLSSTISE 289 >AGl1788 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 134 Score = 124 bits (312), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 0/295 (0%), Positives = 0/295 (0%), Gaps = 23/295 (7%) Query: 9 KLKGIYVLAITPMMDTQAFDPSALRRNIDHYIASGAHGIIVGGTYAEYPSLTSDERKQLF 68 K + IT A + + + Q Sbjct: 2 KHTIAVLGLITFSSPAFAASCEKNFTVSGVPMVTAVSYKSFQELPKAKAPAVLQKLAQAV 61 Query: 69 TXXXXXXXGRVPLLCCTAASGTDEASELAHAAKLAGADGVMV-TPPYVSEVRPQDILYHF 127 L A S ++ V ++ I Sbjct: 62 AAEGFSGIQINKALSSIDAHQETSGSGRIQTLRVVARQKGAAVRIDAVFNIQAGQIA-DK 120 Query: 128 QLLNESAELPILIYN-SASIGVHLSPEEIAGLAKLDNVAGVKQGATD---LHSLVRTVAY 183 Sbjct: 121 - 179 Query: 184 AGNDISVMCGSDGLILGGLASGLPGCTSTNANFMTSEFVALYNEFQQGEN---ARALERY 240 Sbjct: 180 ----- 234 Query: 241 YRWQPIRDLAR--KYGQPAMVKAALDIIGLPSGPVRAPFRTLGEDARADIARTLQ 293 Sbjct: 235 ------ 283 >BMEI1301 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 307 Score = 123 bits (308), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 84/274 (30%), Positives = 125/274 (45%), Gaps = 4/274 (1%) Query: 10 LKGIYVLAITPMMDTQAFDPSALRRNIDHYIASGAHGIIVGGTYAEYPSLTSDERKQLFT 69 LKG +TP AFD A R ++ I G G++ GT E P+L+ DE K++ Sbjct: 16 LKGSITALVTPFDREGAFDEKAFRAFVNWQIEEGTKGLVPVGTTGETPTLSHDEHKRVIE 75 Query: 70 XXXXXXXGRVPLLCCTAASGTDEASELAHAAKLAGADGVMVTPPYVSEVRPQDILYHFQL 129 GRVP++ ++ T EA ELA A+ AGAD V+V PY ++ + + HF Sbjct: 76 VCIEVAAGRVPVIAGAGSNNTVEAIELAQHAEKAGADAVLVVTPYYNKPNQRGLYEHFSR 135 Query: 130 LNESAELPILIYN--SASIGVHLSPEEIAGLAK-LDNVAGVKQGATDLHSLVRTVAYAGN 186 + S +P++IYN SI + ++PE + L + N+ GVK + + A G Sbjct: 136 VARSISIPLVIYNIPGRSI-IDMTPETMGALVRDCKNIVGVKDATGKIERVSEQRAICGK 194 Query: 187 DISVMCGSDGLILGGLASGLPGCTSTNANFMTSEFVALYNEFQQGENARALERYYRWQPI 246 + + G D LG A G GC S +N Q G A+ALE R P+ Sbjct: 195 EFIQLSGEDATALGFNAHGGVGCISVTSNIAPRLCAEFQEACQAGNFAKALELQDRLMPL 254 Query: 247 RDLARKYGQPAMVKAALDIIGLPSGPVRAPFRTL 280 P+ K AL +G +R+P T+ Sbjct: 255 HKALFLEPNPSGPKYALSRLGRIENVLRSPMVTI 288 >AGc1883 [EM] COG0329 Dihydrodipicolinate synthase/N-acetylneuraminate lyase Length = 121 Score = 123 bits (308), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 0/244 (0%), Positives = 0/244 (0%), Gaps = 10/244 (4%) Query: 10 LKGIYVLAITPMMDTQAFDPSALRRNIDHYIASGAHGIIVGGTYAEYPSLTSDERKQLFT 69 + A + S G + + ++ Sbjct: 2 TPFAEAVRQLRERKGVTQKEMAAAIGVSPAYLSALEHGKRGKPSFDLLQRIAGYFNIIWD 61 Query: 70 XXXXXXXGRVPLLCCTAASGTDEASELAHAAKLAGADGVMVTPPYVSEVRPQDILYHFQL 129 + A D + P V E+ Sbjct: 62 EAEELFFLAGSSDPKVVIDTVGLPPQYTAFANRLARDIRKLPPSVVEELSAVLQKSRSCD 121 Query: 130 LNESAELPILIYNSASIG-VHLSPEEIAGLAKL-DNVAGVKQGATDLHSLVRTVAYAGND 187 Sbjct: 122 181 Query: 188 ISVMCGSDGLILGGLASGLPGCTSTNANFMTSEFVALYNEFQ----QGENARALERYYRW 243 Sbjct: 182 ---- 237 Query: 244 QPIR 247 Sbjct: 238 241 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,309,595 Number of extensions: 887209 Number of successful extensions: 2500 Number of sequences better than 1.0e-05: 75 Number of HSP's gapped: 2416 Number of HSP's successfully gapped: 75 Length of query: 298 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 192 Effective length of database: 26,153,027 Effective search space: 5021381184 Effective search space used: 5021381184 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)