BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0032.p (261 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PAE0797 [IQR] COG1028 Dehydrogenases with different specificitie... 199 3e-51 STM1169 [R] COG0673 Predicted dehydrogenases and related proteins 178 7e-45 TM1169 [IQR] COG1028 Dehydrogenases with different specificities... 178 7e-45 VNG2158G [IQR] COG1028 Dehydrogenases with different specificiti... 173 2e-43 APE0912 [IQR] COG1028 Dehydrogenases with different specificitie... 172 5e-43 >PAE0797 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 260 Score = 199 bits (507), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 103/250 (41%), Positives = 151/250 (60%), Gaps = 1/250 (0%) Query: 8 KTALVAAASKGLGRAVAFELARGGAKVALFSRNXXXXXXXXXXXXXXXGMPSLGLQADVC 67 + ++V AAS+G+G VA LAR G+ + + +RN G+ + +QAD+ Sbjct: 6 RVSIVTAASRGIGAGVATVLAREGSNLVIAARNLERLEGLAKQLSSQYGVSVVPVQADLT 65 Query: 68 SASDLSRCVEETFSAFGGLHVLVTNAGGPPAGNFESLKEEDWKYAYELTVLSVVRLIRDA 127 D+ + V+E FG + +L N G P G F L E+DW Y L ++S V + +D Sbjct: 66 RREDVRKIVDEALRHFGKVDILAYNTGPPKPGTFLELTEDDWDYGVRLLLMSAVWITKDV 125 Query: 128 LPHLKEQ-GGAIVNLASSSVKQPIPGLTLSNVMRTGVAGLSKTLAEELAPYGIRVNTVAP 186 LP++ E+ G ++ + SS++KQPIP LTLSNV+R +AGL KTLA +L Y I VN + Sbjct: 126 LPYMIERRWGRLIYITSSTLKQPIPTLTLSNVIRISLAGLVKTLAYQLGKYNILVNGIMQ 185 Query: 187 GRIDTDRVRSLDRIRADKAGVEPEAIKAQSERQIPLGRYGQPDEFARVVAFLCSDGASYV 246 G IDT+RVR + RA + G + I + ER+IPLGR G+P+E +VAFL S+ ASY+ Sbjct: 186 GYIDTERVREVAEARAKREGRSLDEILRELEREIPLGRIGKPEEIGELVAFLASEKASYI 245 Query: 247 TGQTLLVDGG 256 TG +L+DGG Sbjct: 246 TGSLILIDGG 255 >STM1169 [R] COG0673 Predicted dehydrogenases and related proteins Length = 307 Score = 178 bits (452), Expect = 7e-45, Method: Compositional matrix adjust. Identities = 20/253 (7%), Positives = 43/253 (16%), Gaps = 27/253 (10%) Query: 8 KTALVAAASKGLGRAVAFELARGGAKVALFSRNXXXXXXXXXXXXXXXGMPSLGLQADVC 67 L A K + SR+ + Sbjct: 8 IVGLGGIAQKAWLPVLTNTAGWTLQGAWSPSRDKAL-------------RICESWRIPYV 54 Query: 68 SASDLSRCVEETFSAFGGLHVLVTNAGGPPAGNFESLKEEDWKYAYELTVLSVVRLIRDA 127 D + + A ++ + K E + + A Sbjct: 55 ---DSLANLASSCDAVFVHSSTASHYAVVSELLNAGVHVCVDKPLAENLRDAERLVALAA 111 Query: 128 LPHLKEQG-GAIVNLASSSVKQPIPGLTLSNVMRTGVAGLSKTLAEELAPYGIRVNTVAP 186 L + G S M ++ V Sbjct: 112 QKKLTLMVGFNRRFAPLYRELKTRLGTAASLRMDKHRTDSVGPHDLRFTLLDDYLHVVDT 171 Query: 187 GRIDTDRVRSLDRIRADKAGVEPEAIKAQSERQIPLGRYGQPDEFARVVAFLCSDGASYV 246 L + ++ + + ++ + S Sbjct: 172 ALWLAGGEARL----------ASGTLLTSESGEMCYAEHHFSADKLQITTSMHRRAGSQR 221 Query: 247 TGQTLLVDGGMVK 259 + DGG+ Sbjct: 222 ESVQAVTDGGLYD 234 >TM1169 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 237 Score = 178 bits (452), Expect = 7e-45, Method: Compositional matrix adjust. Identities = 108/253 (42%), Positives = 149/253 (58%), Gaps = 27/253 (10%) Query: 8 KTALVAAASKGLGRAVAFELARGGAKVALFSRNXXXXXXXXXXXXXXXGMPSLGLQADVC 67 K LV AAS+G+GRAVA L++ GA+V + +RN + G + VC Sbjct: 8 KGVLVLAASRGIGRAVADVLSQEGAEVTICARNEEL-------------LKRSGHRYVVC 54 Query: 68 SASDLSRCVEETFSAFGGLHVLVTNAGGPPAGNFESLKEEDWKYAYELTVLSVVRLIRDA 127 DL + ++ F + +LV NAGGP AG F+ L ED+K A + L++++++R+ Sbjct: 55 ---DLRKDLDLLFEKVKEVDILVLNAGGPKAGFFDELTNEDFKEAIDSLFLNMIKIVRNY 111 Query: 128 LPHLKEQG-GAIVNLASSSVKQPIPGLTLSNVMRTGVAGLSKTLAEELAPYGIRVNTVAP 186 LP +KE+G G IV + S SV PI L SN R + G KTL+ E+APYGI VN VAP Sbjct: 112 LPAMKEKGWGRIVAITSFSVISPIENLYTSNSARMALTGFLKTLSFEVAPYGITVNCVAP 171 Query: 187 GRIDTDRVRSLDRIRADKAGVEPEAIKAQSERQIPLGRYGQPDEFARVVAFLCSDGASYV 246 G +T+RV+ L E K Q E QIP+ R +P+E A VVAFLCS+ ASY+ Sbjct: 172 GWTETERVKEL----------LSEEKKKQVESQIPMRRMAKPEEIASVVAFLCSEKASYL 221 Query: 247 TGQTLLVDGGMVK 259 TGQT++VDGG+ K Sbjct: 222 TGQTIVVDGGLSK 234 >VNG2158G [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 261 Score = 173 bits (439), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 108/256 (42%), Positives = 144/256 (56%), Gaps = 11/256 (4%) Query: 7 GKTALVAAASKGLGRAVAFELARGGAKVALFSRNXXXXXXXXXXXXXXXGMPSLGLQADV 66 G A+V A+S GLG+A A LAR GA V + SR+ G+ AD+ Sbjct: 7 GDVAVVTASSSGLGKASAAALAREGADVVVCSRDEERVVAAAEEIGADAAGSVRGVPADI 66 Query: 67 CSASDLSRCVEETFSAFGGLHVLVTNAGGPPAGNFESLKEEDWKYAYELTVLSVVRLIRD 126 +D++ VE FGGL +VT+AGG P+G F ++ ++DW A+EL V+SVVR + Sbjct: 67 TDGADVAAVVETAVETFGGLDHVVTSAGGVPSGQFTAVTDDDWTDAFELLVMSVVRTLDA 126 Query: 127 ALPHLKE-QGGAIVNLASSSVKQPIPGLTLSNVMRTGVAGLSKTLAEELAPYGIRVNTVA 185 A HL GG + + S+SV +PI GL LSN +R GV GL+KT+A E AP +R N V Sbjct: 127 AHEHLVAGDGGTVTCITSTSVSEPIEGLVLSNAVRRGVVGLAKTVAREWAP-DVRANVVQ 185 Query: 186 PGRIDTDRVRSL-----DRIRADKAGVEPEAIKAQSERQIPLGRYGQPDEFARVVAFLCS 240 PG +T R+ L R AD EA A ++PL R G+P E VVAFL S Sbjct: 186 PGPHETARIEELVSAAVARGEADSY----EAGLAGWSEEVPLDRLGEPSELGDVVAFLAS 241 Query: 241 DGASYVTGQTLLVDGG 256 D AS+VTG ++ VDGG Sbjct: 242 DRASFVTGASVPVDGG 257 >APE0912 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 260 Score = 172 bits (436), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 109/253 (43%), Positives = 146/253 (57%), Gaps = 5/253 (1%) Query: 7 GKTALVAAASKGLGRAVAFELARGGAKVALFSRNXXXXXXXXXXXXXXXGMPSLGLQA-D 65 GK A+V A S GLG A A ELAR GA++ LFSRN + + A D Sbjct: 7 GKLAVVTAGSSGLGFASALELARNGARLLLFSRNREKLEAAASRIASLVSGAQVDIVAGD 66 Query: 66 VCSASDLSRCVEETFSAFGGLHVLVTNAGGPPAGNFESLKEEDWKYAYELTVLSVVRLIR 125 + D+ R E+ GG +LV + GGP G F L EDW +Y L S V + R Sbjct: 67 IREPGDIDRLFEKARD-LGGADILVYSTGGPRPGRFMELGVEDWDESYRLLARSAVWVGR 125 Query: 126 DALPHLKEQG-GAIVNLASSSVKQPIPGLTLSNVMRTGVAGLSKTLAEELAPYGIRVNTV 184 A + E+G G +V + S ++ +P L LSN+MR V G+ +TLA ELAP+G+ VN V Sbjct: 126 RAAEQMVEKGWGRMVYIGSVTLLRPWQDLALSNIMRLPVIGVVRTLALELAPHGVTVNAV 185 Query: 185 APGRIDTDRVRSLDRIRADKAGVE-PEAIKAQSERQIPLGRYGQPDEFARVVAFLCSDGA 243 P I TDRVRSL RA ++G+ EA+K+ + R IP+GR G+P+E A VVAFL S+ A Sbjct: 186 LPSLILTDRVRSLAEERARRSGITVEEALKSMASR-IPMGRVGKPEELASVVAFLASEKA 244 Query: 244 SYVTGQTLLVDGG 256 S++TG + VDGG Sbjct: 245 SFITGAVIPVDGG 257 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,468,676 Number of extensions: 674404 Number of successful extensions: 4170 Number of sequences better than 1.0e-05: 309 Number of HSP's gapped: 3553 Number of HSP's successfully gapped: 309 Length of query: 261 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 157 Effective length of database: 26,383,695 Effective search space: 4142240115 Effective search space used: 4142240115 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)