BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0030.p (271 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF0115 [R] COG0388 Predicted amidohydrolase 145 9e-35 jhp0294 [R] COG0388 Predicted amidohydrolase 144 2e-34 PAB1449 [R] COG0388 Predicted amidohydrolase 143 3e-34 Cj0947c [R] COG0388 Predicted amidohydrolase 143 3e-34 SPCC965.09 [R] COG0388 Predicted amidohydrolase 140 2e-33 >AF0115 [R] COG0388 Predicted amidohydrolase Length = 257 Score = 145 bits (365), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 86/261 (32%), Positives = 137/261 (52%), Gaps = 14/261 (5%) Query: 5 ETVKLALVQFESALMDVAANVRKGLRFVEQASRQGAGLIVFPELFVTGYNTDVISHRYHE 64 E +++AL Q + L D N+ KG+ +++A + A +++ PE+F TG+ H Y Sbjct: 5 EKIRIALAQ-QRILPDREVNIMKGMSLIKRAIQVRADMVILPEVFNTGFY----KHNY-- 57 Query: 65 LAEDIDGPTVWAFREAAQTHNINIVIPMALKQSEGGTANGAVIIDRSGAIAGTYSKVHLW 124 E ++ + +I+I + + EG + +I G I G Y K HL+ Sbjct: 58 --ETVEPLEEELSLLLKISEQKDIMIITGVAEREGDDLYNSAVIIHKGKIIGKYRKTHLF 115 Query: 125 --EDERKYFKPGDICAPYELDFGKLGVLICYDAGFPEAARTLALQGVKLIVTPSAFSLED 182 +E+KYFK GD +E GK+G+LICY+ FPE +R L G ++IV P+ F E Sbjct: 116 PLTNEKKYFKAGDKLEVFETHLGKIGLLICYEVRFPELSRKLVKMGAEIIVIPAEFPKER 175 Query: 183 KHRWDIYFPARALENTCFVAGINGVGSEGGLRLFGNNKLANPRGDLILNGDLNEEAMQVL 242 W + ARA+EN FVAG+N V EG L G++ L +P G +++ +E M + Sbjct: 176 IDHWRVLLQARAIENQVFVAGVNCV--EGDLDYGGHSMLIDPMGTVLIEASEYQEVM-MS 232 Query: 243 EIDLNEAAECSRQIPYLRDLR 263 +I L + E ++ P+L DLR Sbjct: 233 DIRLGDVYEVRKKFPFLNDLR 253 >jhp0294 [R] COG0388 Predicted amidohydrolase Length = 294 Score = 144 bits (362), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 93/278 (33%), Positives = 144/278 (51%), Gaps = 29/278 (10%) Query: 7 VKLALVQFESALMDVAANVRKGLRFVEQASRQGAGLIVFPELFVTGYNTD---------- 56 +K A++Q +S + N++ L ++A +GA LIV PELF +GY + Sbjct: 11 LKTAVIQMQSKPYALNENLQLALNLAKEAHNKGANLIVLPELFDSGYCVNDKDADFGLDF 70 Query: 57 -VISHRYHELAEDIDGPTVWAFREAAQTHNINIVIPMALKQSEGGTANGAVIIDRSGAIA 115 I H E + T+ A + A++ + +IV +++++ + A II G I Sbjct: 71 KAIEHG----EETLKNETLRALSDFAKSSDTHIV-ACSIEKNNKKLYDSAYIIPPKGKIV 125 Query: 116 GTYSKVHLWEDERKYFKPGDICAPYELDFG----KLGVLICYDAGFPEAARTLALQGVKL 171 G + K++LW DE+ FK G + LDFG K+G+ ICY+ GF A L LQG ++ Sbjct: 126 GKHRKIYLWGDEKSRFKRGKKYEVFTLDFGDFSAKVGLQICYETGFGVGANLLVLQGAEV 185 Query: 172 IVTPSAFSLEDKHRWDIYFPARALENTCFVAGINGVGSEGGLRL------FGNNKLANPR 225 ++ PSAF + WD+ ARALEN CFV N G E +L G++++ P Sbjct: 186 LIYPSAFGKARAYNWDLLSKARALENGCFVCACNHSGEETNAKLKQTLEFAGDSRIIAPN 245 Query: 226 GDLILNG-DLNEEAMQVLEIDLNEAAECSRQIPYLRDL 262 G +I LNE + + E+DLNE A ++IPYL+D Sbjct: 246 GKIIAQATKLNE--VIIAEMDLNEVALQRQKIPYLQDF 281 >PAB1449 [R] COG0388 Predicted amidohydrolase Length = 262 Score = 143 bits (360), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 89/242 (36%), Positives = 137/242 (56%), Gaps = 8/242 (3%) Query: 7 VKLALVQFESALMDVAANVRKGLRFVEQASRQGAGLIVFPELFVTGYNTDVISHRYHELA 66 VK+A VQ +++ N K + +++AS+QGA L+V PELF TGYN + + E+A Sbjct: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVF-EIA 60 Query: 67 EDI-DGPTVWAFREAAQTHNINIVIPMALKQSEGGTANGAVIIDRSGAIAGTYSKVHLWE 125 + I +G T + A+ + IV A K + N AV++ G I G Y K+HL+ Sbjct: 61 QKIPEGETTTFLMDVARDTGVYIVAGTAEKDGD-VLYNSAVVVGPRGFI-GKYRKIHLFY 118 Query: 126 DERKYFKPGDI-CAPYELDFGKLGVLICYDAGFPEAARTLALQGVKLIVTPSAFSLEDKH 184 E+ +F+PGD+ ++L F K+GV+IC+D FPE+ARTLAL+G +I P+ + Sbjct: 119 REKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAP 178 Query: 185 RWDIYFPARALENTCFVAGINGVGSEGGLRLFGNNKLANPRGDLILNGDLNEEAMQVLEI 244 R P RALEN + + VG E GL+ G + +A+P+ +++ EE + V EI Sbjct: 179 RA---MPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEI 235 Query: 245 DL 246 DL Sbjct: 236 DL 237 >Cj0947c [R] COG0388 Predicted amidohydrolase Length = 290 Score = 143 bits (360), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 90/284 (31%), Positives = 150/284 (52%), Gaps = 26/284 (9%) Query: 7 VKLALVQFESALMDVAANVRKGLRFVEQASRQGAGLIVFPELFVTGYNTDVISHRYHELA 66 +K+AL+Q + ++ ++K F+E+AS+QGA L+ EL + Y + + + A Sbjct: 1 MKIALIQ-QKFHLNKEQTIKKTCEFIEEASKQGAELVCLGELHQSEYFCQSENVDFFDYA 59 Query: 67 EDIDGPT-VWAFREAAQTHNINIVIPMALKQSEGGTANGAVIIDRSGAIAGTYSKVHLWE 125 D + WA A+ + I ++ + K+S G N AV+ ++ G+IAG Y K+H+ + Sbjct: 60 NDYEKDVKFWA--NIARKNQIVLIASLFEKRSAGLYHNTAVVFEKDGSIAGKYRKMHIPD 117 Query: 126 D----ERKYFKPGDI-CAPYELDFGKLGVLICYDAGFPEAARTLALQGVKLIVTPSAFSL 180 D E+ YF PGD+ P GKLGVLIC+D +PEAAR +AL+G ++++ P+A Sbjct: 118 DPCFYEKFYFTPGDLGFEPINTSLGKLGVLICWDQWYPEAARIMALKGAEILIYPTAIGW 177 Query: 181 EDKHR----------WDIYFPARALENTCFVAGINGVGSEG-------GLRLFGNNKLAN 223 DK + W A+ N +V IN VG E G+R +GN+ + Sbjct: 178 FDKDKDEEKQRQLNAWLGVQKGHAIANGLYVVAINRVGFEKDVSGVEEGIRFWGNSFVFG 237 Query: 224 PRGDLILNGDLNEEAMQVLEIDLNEAAECSRQIPYLRDLRMDTY 267 P+G+ + D E ++++EID + R P+LRD R++ + Sbjct: 238 PQGEELCLLDSQNECVKIIEIDKKRSENVRRWWPFLRDRRIEYF 281 >SPCC965.09 [R] COG0388 Predicted amidohydrolase Length = 272 Score = 140 bits (353), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 84/260 (32%), Positives = 129/260 (49%), Gaps = 6/260 (2%) Query: 9 LALVQFESALMDVAANVRKGLRFVEQA--SRQGAGLIVFPELFVTGYNTDVISHRYHELA 66 +A VQ + DV N++K +V + S LI+FPEL +GY + E+A Sbjct: 5 IACVQMAPKVCDVKHNLQKMSSYVHEVMESNPSTNLILFPELITSGYECGNTFTQIAEIA 64 Query: 67 EDIDGPTVWAFREAAQTHNINIV--IPMALKQSEGGTANGAVIIDRSGAIAGTYSKVHLW 124 + GP+ A +++NI+ P ++ N + I +G + G Y KVHL+ Sbjct: 65 GE--GPSFKTMSNLAAKYHVNIIYGFPEKEEKQSNIIYNSCIYITENGNLGGVYRKVHLF 122 Query: 125 EDERKYFKPGDICAPYELDFGKLGVLICYDAGFPEAARTLALQGVKLIVTPSAFSLEDKH 184 + ERK+FK G +E FGKLGV+IC+D FPE AR AL G L+V + + Sbjct: 123 DTERKHFKKGSDFPIFETSFGKLGVMICWDTAFPEVARIHALNGADLLVVATNWENPYSD 182 Query: 185 RWDIYFPARALENTCFVAGINGVGSEGGLRLFGNNKLANPRGDLILNGDLNEEAMQVLEI 244 WD+ ARA EN + N VG++ L FG++K+ P G +I D +E + + Sbjct: 183 DWDLVTKARAFENCIPLVAANRVGTDEKLSFFGHSKIIGPTGKVIKALDEEKEGVISYTV 242 Query: 245 DLNEAAECSRQIPYLRDLRM 264 DL++A + + RM Sbjct: 243 DLDDAKPLRKNYYTFFEDRM 262 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,732,558 Number of extensions: 886982 Number of successful extensions: 1966 Number of sequences better than 1.0e-05: 95 Number of HSP's gapped: 1804 Number of HSP's successfully gapped: 96 Length of query: 271 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 166 Effective length of database: 26,268,361 Effective search space: 4360547926 Effective search space used: 4360547926 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)