BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0010.p (291 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC3032 [G] COG2017 Galactose mutarotase and related enzymes 241 1e-63 lin1322 [G] COG2017 Galactose mutarotase and related enzymes 225 8e-59 mlr0411 [G] COG2017 Galactose mutarotase and related enzymes 176 4e-44 L183012 [G] COG2017 Galactose mutarotase and related enzymes 166 3e-41 L74167 [G] COG2017 Galactose mutarotase and related enzymes 145 7e-35 >CAC3032 [G] COG2017 Galactose mutarotase and related enzymes Length = 346 Score = 241 bits (614), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 18/297 (6%), Positives = 56/297 (18%), Gaps = 8/297 (2%) Query: 1 MNTILRSGSARAEISSLGAELVSFTKNDTGTEYIWSGDAAYWTGRSPVLFPIIGAVRNGE 60 M S+ + + +N + A + + E Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 61 IKVDGQTYKLANHGFARRSEFTIVEADESRAVFLLTSSGHTLSSYPFQFNLYITYTLNES 120 + +TY E I+ + S + + + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 121 TLKLEYRVENTDGQNIFFQLGTHPAFNCPL------DENGSFSDYVLEFSENETLDREFL 174 + ++ + + + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 175 NSAGLRISGRSEAVLSGERILPLTHEMFKDDALVFQNVASRTITLKSKLTEKSVAVSFEG 234 +A + + +T + V ++ + + + G Sbjct: 181 ETAFG-VMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFG 239 Query: 235 FPDLGIWQKPN-APFLCIEPWQGFADSDEFQGELSEKEGIVSLAPGDNFTSSLTIKI 290 A + S E + ++ G + + Sbjct: 240 TLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFV 296 >lin1322 [G] COG2017 Galactose mutarotase and related enzymes Length = 553 Score = 225 bits (573), Expect = 8e-59, Method: Compositional matrix adjust. Identities = 14/281 (4%), Positives = 47/281 (16%), Gaps = 4/281 (1%) Query: 12 AEISSLGAELVSFTKNDTGTEYIWSGDAAYWTGRSPVLFPIIGAVRNGEIKVDGQTYKLA 71 + L+ + ++ + + Sbjct: 11 LVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTRINLSRS 70 Query: 72 NHGFARRSEFTIVEADESRAVFLLTSSGHTLSSYPFQFNLYITYTLNESTLKLEYRVENT 131 S A + + Y ++ + ++ ++ + +N Sbjct: 71 AVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNY 130 Query: 132 DGQNIFFQLGTHPAFNCPLDENGSFSDYVLEFSENETLDREFLNSAGLRISGRSEAVLS- 190 + + R A Sbjct: 131 YTALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQY--ALSSEKLYRDIVTDNADDYR 188 Query: 191 GERILPLTHEMFKDDALVFQNVASRTITLKSKLTEKSVAVSFEGFPDLGIW-QKPNAPFL 249 + + L+ R + L + G + Sbjct: 189 FAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMG 248 Query: 250 CIEPWQGFADSDEFQGELSEKEGIVSLAPGDNFTSSLTIKI 290 + +EG ++ G ++ + Sbjct: 249 DLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDL 289 >mlr0411 [G] COG2017 Galactose mutarotase and related enzymes Length = 553 Score = 176 bits (446), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 16/281 (5%), Positives = 53/281 (18%), Gaps = 6/281 (2%) Query: 12 AEISSLGAELVSFTKNDTGTEYIWSGDAAYWTGRSPVLFPIIGAVRNGEIKVDGQTYKLA 71 + +L + + + + + Sbjct: 15 LGVFALLQLISGS-LFFSSLHHSQKS-FVVSNQLREQQGELTSTWDLMLQTRINLSRSAV 72 Query: 72 NHGFARRSEFTIVEADESRAVFLLTSSGHTLSSYPFQFNLYITYTLNESTLKLEYRVENT 131 ++ + + + + + T + +Y+ T Sbjct: 73 RMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNYYT 132 Query: 132 DGQNIFFQLGTHPAFNCPLDENGSFSDYVLEFSENETLDREFLNSAGLRISGRSEAVLSG 191 + L + + E L E L + + + Sbjct: 133 ALTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYR-DIVTDNADDYRFAQ 191 Query: 192 ERILPLTHEMFKDDALVFQNVASRTITLKSKLTEKSVAVSFEGFPDLGIWQKP-NAPFLC 250 ++ + + LV R + + G + Sbjct: 192 WQLA-VIALVVVLILLVAWYGIRRMLLTPLA-KIIAHIREIAGGNLANTLTIDGRSEMGD 249 Query: 251 IEPWQGFADSDEFQGELSEKEGIVSLAPGDNFTSSLTIKID 291 + +EG ++ G ++ + Sbjct: 250 LAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLS 290 >L183012 [G] COG2017 Galactose mutarotase and related enzymes Length = 553 Score = 166 bits (421), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 11/243 (4%), Positives = 48/243 (19%), Gaps = 12/243 (4%) Query: 1 MNTILRSGSARAEISSLGAELVSFTKNDTGTEYIWSGDAAYWTGRSPVLFPIIGAVRNGE 60 M +R + + + A L + + + + + + Sbjct: 1 MINRIRVVTLLVMVLGVFALLQLISGSLF--FSSLHHSQKSFVVSNQLREQQGELTSTWD 58 Query: 61 IKVDGQTYKLANHGFARRSEFTIVEADESRAVFLLTSSGHTLSSYPFQFNLYITYTLNES 120 + + + + + + + +++ +F + Sbjct: 59 LMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVA 118 Query: 121 TLKLEYRVENTDGQNIFFQLGTHPAFNCPLDENGSFSDYVLEFSENETLDREFL----NS 176 T + + + N + + ++ Sbjct: 119 TSRNIDEKYKNYYTALTELIDYLDYGNTGA-YFAQPTQGMQNAMGEAF--AQYALSSEKL 175 Query: 177 AGLRISGRSEAVLSGERILPLTHEMFKDDALVFQNVASRTITLKSKLTEKSVAVSFEGFP 236 ++ ++ + L+ + K +A E Sbjct: 176 YRDIVTDNADDYRFA---QWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAG 232 Query: 237 DLG 239 Sbjct: 233 GNL 235 >L74167 [G] COG2017 Galactose mutarotase and related enzymes Length = 553 Score = 145 bits (366), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 16/288 (5%), Positives = 49/288 (17%), Gaps = 11/288 (3%) Query: 5 LRSGSARAEISSLGAELVSFTKNDTGTEYIWSGDAAYWTGRSPVLFPIIGAVRNGEIKVD 64 + + +L + + S + Sbjct: 8 VTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVV--SNQLREQQGELTSTWDLMLQTRI 65 Query: 65 GQTYKLANHGFARRSEFTIVEADESRAVFLLTSSGHTLSSYPFQFNLYITYTLNESTLKL 124 + ++ + + + + + T + Sbjct: 66 NLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNIDE 125 Query: 125 EYRVENTDGQNIFFQLGTHPAFNCPLDENGSFSDYVLEF-SENETLDR-EFLNSAGLRIS 182 +Y+ T + L + + E ++ Sbjct: 126 KYKNYYTALTELIDYLDYGNTGAY----FAQPTQGMQNAMGEAFAQYALSSEKLYRDIVT 181 Query: 183 GRSEAVLSGERILPLTHEMFKDDALVFQNVASRTITLKSKLTEKSVAVSFEGFPDLGIWQ 242 ++ + L + + LV R + L + G Sbjct: 182 DNADDYRFAQWQLAVIALVVVLILLVAWYGIRR-MLLTPLAKIIAHIREIAGGNLANTLT 240 Query: 243 K--PNAPFLCIEPWQGFADSDEFQGELSEKEGIVSLAPGDNFTSSLTI 288 + + S + A + T Sbjct: 241 IDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTD 288 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,421,156 Number of extensions: 885679 Number of successful extensions: 1429 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 1417 Number of HSP's successfully gapped: 7 Length of query: 291 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 186 Effective length of database: 26,268,361 Effective search space: 4885915146 Effective search space used: 4885915146 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)