BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0008.p (377 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_aldY [C] COG1012 NAD-dependent aldehyde dehydrogenases 532 e-151 BS_aldX [C] COG1012 NAD-dependent aldehyde dehydrogenases 532 e-151 BH2329 [E] COG0686 Alanine dehydrogenase 503 e-142 BH3180 [E] COG0686 Alanine dehydrogenase 454 e-127 SA1531 [E] COG0686 Alanine dehydrogenase 435 e-122 >BS_aldY [C] COG1012 NAD-dependent aldehyde dehydrogenases Length = 378 Score = 532 bits (1371), Expect = e-151, Method: Compositional matrix adjust. Identities = 267/371 (71%), Positives = 301/371 (81%), Gaps = 1/371 (0%) Query: 1 MIIGVPKEIKNNENRVAITPAGVVSLVADGHKVLVEAGAGTGSGFLNEEYAAAGAELITE 60 MIIGVPKEIKNNENRVA+TP GV L+++GH+VLVE GAG GSGF NE Y +AGAE+I + Sbjct: 1 MIIGVPKEIKNNENRVALTPGGVSQLISNGHRVLVETGAGLGSGFENEAYESAGAEIIAD 60 Query: 61 ASAVWAAAEMVMKVKEPLESEYSYFRPGLILFTYLHLAPEPTLASALKDNGVFAIGYETV 120 VW A EMVMKVKEPL EY YFR GL+LFTYLHLA EP LA ALKD GV AI YETV Sbjct: 61 PKQVWDA-EMVMKVKEPLPEEYVYFRKGLVLFTYLHLAAEPELAQALKDKGVTAIAYETV 119 Query: 121 VDGRTLPLLTPMSEVAGRMSVQLGAQFLQKNYGGQGILLSGVPXXXXXXXXXXXXXXXXT 180 +GRTLPLLTPMSEVAGRM+ Q+GAQFL+K GG+GILL+GVP T Sbjct: 120 SEGRTLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLAGVPGVSRGKVTIIGGGVVGT 179 Query: 181 NAAKMAIGLGAEVTIVDLSADRLRQLDDIFGAQISTLISNPYNIAKAVAEADLLIGAVLI 240 NAAKMA+GLGA+VTI+DL+ADRLRQLDDIFG QI TLISNP NIA AVAEADLLI AVLI Sbjct: 180 NAAKMAVGLGADVTIIDLNADRLRQLDDIFGHQIKTLISNPVNIADAVAEADLLICAVLI 239 Query: 241 PGAKAPKLVTEEMVKTMKPGSVIVDVAIDQGGIVETIDRVTTHDHPVFEKYGVLHYSVAN 300 PGAKAP LVTEEMVK MKPGSVIVDVAIDQGGIVET+D +TTHD P +EK+GV+HY+VAN Sbjct: 240 PGAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDQPTYEKHGVVHYAVAN 299 Query: 301 MPGAVAKTSTMALTNVTVPYALQIANKGVFKAIEDNAGLKSGVNVANGRITCRAVAEALG 360 MPGAV +TST+ALTNVTVPYALQIANKG KA+ DN L++G+N ANG +T AVA LG Sbjct: 300 MPGAVPRTSTIALTNVTVPYALQIANKGAVKALADNTALRAGLNTANGHVTYEAVARDLG 359 Query: 361 EEYYTVEQALE 371 EY E+AL+ Sbjct: 360 YEYVPAEKALQ 370 >BS_aldX [C] COG1012 NAD-dependent aldehyde dehydrogenases Length = 378 Score = 532 bits (1371), Expect = e-151, Method: Compositional matrix adjust. Identities = 267/371 (71%), Positives = 301/371 (81%), Gaps = 1/371 (0%) Query: 1 MIIGVPKEIKNNENRVAITPAGVVSLVADGHKVLVEAGAGTGSGFLNEEYAAAGAELITE 60 MIIGVPKEIKNNENRVA+TP GV L+++GH+VLVE GAG GSGF NE Y +AGAE+I + Sbjct: 1 MIIGVPKEIKNNENRVALTPGGVSQLISNGHRVLVETGAGLGSGFENEAYESAGAEIIAD 60 Query: 61 ASAVWAAAEMVMKVKEPLESEYSYFRPGLILFTYLHLAPEPTLASALKDNGVFAIGYETV 120 VW A EMVMKVKEPL EY YFR GL+LFTYLHLA EP LA ALKD GV AI YETV Sbjct: 61 PKQVWDA-EMVMKVKEPLPEEYVYFRKGLVLFTYLHLAAEPELAQALKDKGVTAIAYETV 119 Query: 121 VDGRTLPLLTPMSEVAGRMSVQLGAQFLQKNYGGQGILLSGVPXXXXXXXXXXXXXXXXT 180 +GRTLPLLTPMSEVAGRM+ Q+GAQFL+K GG+GILL+GVP T Sbjct: 120 SEGRTLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLAGVPGVSRGKVTIIGGGVVGT 179 Query: 181 NAAKMAIGLGAEVTIVDLSADRLRQLDDIFGAQISTLISNPYNIAKAVAEADLLIGAVLI 240 NAAKMA+GLGA+VTI+DL+ADRLRQLDDIFG QI TLISNP NIA AVAEADLLI AVLI Sbjct: 180 NAAKMAVGLGADVTIIDLNADRLRQLDDIFGHQIKTLISNPVNIADAVAEADLLICAVLI 239 Query: 241 PGAKAPKLVTEEMVKTMKPGSVIVDVAIDQGGIVETIDRVTTHDHPVFEKYGVLHYSVAN 300 PGAKAP LVTEEMVK MKPGSVIVDVAIDQGGIVET+D +TTHD P +EK+GV+HY+VAN Sbjct: 240 PGAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDQPTYEKHGVVHYAVAN 299 Query: 301 MPGAVAKTSTMALTNVTVPYALQIANKGVFKAIEDNAGLKSGVNVANGRITCRAVAEALG 360 MPGAV +TST+ALTNVTVPYALQIANKG KA+ DN L++G+N ANG +T AVA LG Sbjct: 300 MPGAVPRTSTIALTNVTVPYALQIANKGAVKALADNTALRAGLNTANGHVTYEAVARDLG 359 Query: 361 EEYYTVEQALE 371 EY E+AL+ Sbjct: 360 YEYVPAEKALQ 370 >BH2329 [E] COG0686 Alanine dehydrogenase Length = 378 Score = 503 bits (1296), Expect = e-142, Method: Compositional matrix adjust. Identities = 252/370 (68%), Positives = 298/370 (80%) Query: 1 MIIGVPKEIKNNENRVAITPAGVVSLVADGHKVLVEAGAGTGSGFLNEEYAAAGAELITE 60 MIIGVPKEIKNNENRVA+TPA V+S V GH+VL+E GAG+GSGF + +Y AGA + + Sbjct: 1 MIIGVPKEIKNNENRVAMTPASVMSFVQAGHQVLIERGAGSGSGFDDIDYGEAGATIEND 60 Query: 61 ASAVWAAAEMVMKVKEPLESEYSYFRPGLILFTYLHLAPEPTLASALKDNGVFAIGYETV 120 A++VWA A+M+MKVKEPL EY YFR GL+LFTYLHLA EP LA AL +N V AI YETV Sbjct: 61 AASVWAKADMIMKVKEPLPQEYGYFRKGLLLFTYLHLAAEPELAKALVENEVTAIAYETV 120 Query: 121 VDGRTLPLLTPMSEVAGRMSVQLGAQFLQKNYGGQGILLSGVPXXXXXXXXXXXXXXXXT 180 GR+LPLLTPMSEVAGRM+ Q+GAQFL+K GG+GILLSGVP T Sbjct: 121 QVGRSLPLLTPMSEVAGRMAAQIGAQFLEKPKGGRGILLSGVPGVTRGKVTIIGGGVVGT 180 Query: 181 NAAKMAIGLGAEVTIVDLSADRLRQLDDIFGAQISTLISNPYNIAKAVAEADLLIGAVLI 240 NAAK+A+GLGA+VTIVDLS +RLRQLDD++G I TL+SNP NIA+AVA +DL+IGAVLI Sbjct: 181 NAAKVAMGLGADVTIVDLSPERLRQLDDLYGTDIQTLMSNPMNIAEAVAASDLVIGAVLI 240 Query: 241 PGAKAPKLVTEEMVKTMKPGSVIVDVAIDQGGIVETIDRVTTHDHPVFEKYGVLHYSVAN 300 PGAKAPKLVTE+MV+ MKPGSVIVDVAIDQGGIVET+DR+TTHD P + K+GV+HY+VAN Sbjct: 241 PGAKAPKLVTEQMVEQMKPGSVIVDVAIDQGGIVETVDRITTHDAPTYTKHGVVHYAVAN 300 Query: 301 MPGAVAKTSTMALTNVTVPYALQIANKGVFKAIEDNAGLKSGVNVANGRITCRAVAEALG 360 MPGAV +TST+ALTNVTVPYALQ+AN GV +AI +N L GVN ANG +T AVA LG Sbjct: 301 MPGAVPRTSTIALTNVTVPYALQLANNGVKRAIAENTALAKGVNTANGWVTYEAVARDLG 360 Query: 361 EEYYTVEQAL 370 +Y +VE AL Sbjct: 361 YDYQSVESAL 370 >BH3180 [E] COG0686 Alanine dehydrogenase Length = 371 Score = 454 bits (1168), Expect = e-127, Method: Compositional matrix adjust. Identities = 231/371 (62%), Positives = 279/371 (75%), Gaps = 2/371 (0%) Query: 1 MIIGVPKEIKNNENRVAITPAGVVSLVADGHKVLVEAGAGTGSGFLNEEYAAAGAELITE 60 M IGVP E+KNNENRVAITPAGV++L GH+V+V+ GAG GSGF +E++ AGA+++ Sbjct: 1 MKIGVPTEVKNNENRVAITPAGVMTLTQHGHEVIVQKGAGEGSGFTDEQFVIAGAKIVDT 60 Query: 61 ASAVWAAAEMVMKVKEPLESEYSYFRPGLILFTYLHLAPEPTLASALKDNGVFAIGYETV 120 A VW EMVMKVKEPL EY +FR GLILFTYLHLAPE L AL + V I YETV Sbjct: 61 ADEVWQM-EMVMKVKEPLPEEYGFFREGLILFTYLHLAPELKLTDALLEKKVIGIAYETV 119 Query: 121 -VDGRTLPLLTPMSEVAGRMSVQLGAQFLQKNYGGQGILLSGVPXXXXXXXXXXXXXXXX 179 + LPLLTPMSEVAGRM+ Q+GAQFL+K GG+G+LLSG+P Sbjct: 120 QLANGALPLLTPMSEVAGRMASQIGAQFLEKTQGGKGVLLSGLPGVKRGKVAVIGGGVVG 179 Query: 180 TNAAKMAIGLGAEVTIVDLSADRLRQLDDIFGAQISTLISNPYNIAKAVAEADLLIGAVL 239 TNAAK+A+GLGA+VTI+DL+ DRLRQLDD+FG I+TL+SNP NIA+ V E+DL+IGAVL Sbjct: 180 TNAAKIAVGLGADVTILDLNPDRLRQLDDLFGESINTLMSNPLNIAETVKESDLVIGAVL 239 Query: 240 IPGAKAPKLVTEEMVKTMKPGSVIVDVAIDQGGIVETIDRVTTHDHPVFEKYGVLHYSVA 299 IPGAKAPKLVTEEMV +M+PGSVIVDVAIDQGGIV T DR+TTH +P +EK+GVLHY+VA Sbjct: 240 IPGAKAPKLVTEEMVASMQPGSVIVDVAIDQGGIVATSDRITTHSNPTYEKHGVLHYAVA 299 Query: 300 NMPGAVAKTSTMALTNVTVPYALQIANKGVFKAIEDNAGLKSGVNVANGRITCRAVAEAL 359 NMPGAV +TST+ LTNVT+PYAL IANKG +A D+ L+ G+N G I AVA + Sbjct: 300 NMPGAVPRTSTIGLTNVTIPYALAIANKGYVQACLDDPALRKGLNTLGGYIVYEAVAASQ 359 Query: 360 GEEYYTVEQAL 370 + Y V L Sbjct: 360 NKPYKDVMDLL 370 >SA1531 [E] COG0686 Alanine dehydrogenase Length = 372 Score = 435 bits (1118), Expect = e-122, Method: Compositional matrix adjust. Identities = 218/372 (58%), Positives = 278/372 (74%), Gaps = 2/372 (0%) Query: 1 MIIGVPKEIKNNENRVAITPAGVVSLVADGHKVLVEAGAGTGSGFLNEEYAAAGAELITE 60 M IG+P+EIKNNENRV ++P+GV +LV GH VLVE AG+GS F + +Y AGAE++ E Sbjct: 1 MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSFFEDVDYKEAGAEIVAE 60 Query: 61 ASAVWAAAEMVMKVKEPLESEYSYFRPGLILFTYLHLAPEPTLASALKDNGVFAIGYETV 120 + VW +MV+KVKEPLESEY YF+ GL+LFTYLHLA E L AL D V +I YETV Sbjct: 61 QAKVWDV-DMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKLTQALIDRKVISIAYETV 119 Query: 121 -VDGRTLPLLTPMSEVAGRMSVQLGAQFLQKNYGGQGILLSGVPXXXXXXXXXXXXXXXX 179 + R+ PLL+PMSEVAGRMS Q+GA+FLQK GG GILL GVP Sbjct: 120 QLPDRSSPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLGGVPGVPKGKVTIIGGGQAG 179 Query: 180 TNAAKMAIGLGAEVTIVDLSADRLRQLDDIFGAQISTLISNPYNIAKAVAEADLLIGAVL 239 TNAAK+A+GLGA+VTI+D++ RL+QLDD+FG ++ T++SNP NI V ++DL+IGAVL Sbjct: 180 TNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIMSNPLNIELYVKQSDLVIGAVL 239 Query: 240 IPGAKAPKLVTEEMVKTMKPGSVIVDVAIDQGGIVETIDRVTTHDHPVFEKYGVLHYSVA 299 IPGAKAP+LVTE+M+K MK GSVI+D+AIDQGGI ET D++TTHD P + K+GV+HY+VA Sbjct: 240 IPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFETTDKITTHDDPTYIKHGVVHYAVA 299 Query: 300 NMPGAVAKTSTMALTNVTVPYALQIANKGVFKAIEDNAGLKSGVNVANGRITCRAVAEAL 359 NMPGAV +TST+AL N T+PYAL +ANKG +A + N L G+N G +T + VAEA Sbjct: 300 NMPGAVPRTSTLALNNATLPYALMLANKGYREAFKSNQPLSLGLNTYKGHVTNKGVAEAF 359 Query: 360 GEEYYTVEQALE 371 EY +VE+AL+ Sbjct: 360 EMEYKSVEEALQ 371 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.133 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,068,308 Number of extensions: 1004466 Number of successful extensions: 3931 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 3901 Number of HSP's successfully gapped: 31 Length of query: 377 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 269 Effective length of database: 25,922,359 Effective search space: 6973114571 Effective search space used: 6973114571 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)