BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0126.0015.p (1595 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0279 [U] COG0823 Periplasmic component of the Tol biopolymer ... 80 2e-14 >RSp0279 [U] COG0823 Periplasmic component of the Tol biopolymer transport system Length = 475 Score = 80.5 bits (197), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 54/130 (41%), Positives = 79/130 (60%), Gaps = 6/130 (4%) Query: 848 LAEGKPVMASSAKAADVPPGNAVDG-SLTTRWGS---NYADPQWIYVDLGSSQPIAKVML 903 L++G P ASS+ + + NA DG + TRW S + A QW+ VDLG+ + I V L Sbjct: 346 LSQGMPASASSSYNSSLGAANAFDGDTANTRWDSIEGSAAGTQWLMVDLGTVRTINGVDL 405 Query: 904 HWESAYGKEYQIQVSDNTADWTTVYSTATGDGDIDDI-SFEPVNARYVRMYGTKRATTYG 962 +W++ K Y IQ S + +WT++YST+ G + S + RYVRMYGT+RAT +G Sbjct: 406 YWDAG-AKTYAIQTSSDGVNWTSIYSTSNGIAYGHAVLSNLRGSGRYVRMYGTQRATQWG 464 Query: 963 YSLWEFKVYG 972 YS+ E +V+G Sbjct: 465 YSIDEMQVWG 474 Score = 65.5 bits (158), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 40/92 (43%), Positives = 54/92 (58%), Gaps = 2/92 (2%) Query: 751 NASDKEWIYVDLQDQYTINKVRLNWEKAYGKSYKIQVSDDAVTWTDVYTTSMGDG-GIDD 809 +A+ +W+ VDL TIN V L W+ A K+Y IQ S D V WT +Y+TS G G Sbjct: 383 SAAGTQWLMVDLGTVRTINGVDLYWD-AGAKTYAIQTSSDGVNWTSIYSTSNGIAYGHAV 441 Query: 810 ISFGKVSARFVRMLGVQQGTGDGYSLAEFNVY 841 +S + S R+VRM G Q+ T GYS+ E V+ Sbjct: 442 LSNLRGSGRYVRMYGTQRATQWGYSIDEMQVW 473 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.130 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 112,819,260 Number of extensions: 4746286 Number of successful extensions: 8164 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 8159 Number of HSP's successfully gapped: 2 Length of query: 1595 Length of database: 38,378,431 Length adjustment: 120 Effective length of query: 1475 Effective length of database: 24,538,351 Effective search space: 36194067725 Effective search space used: 36194067725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 123 (52.0 bits)