BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0126.0010.p (759 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0483 [K] COG2207 AraC-type DNA-binding domain-containing proteins 86 2e-16 BS_yesS [K] COG2207 AraC-type DNA-binding domain-containing prot... 77 1e-13 BH1123 [T] COG4753 Response regulator containing CheY-like recei... 69 3e-11 BH3679 [T] COG4753 Response regulator containing CheY-like recei... 67 1e-10 SPy1556 [T] COG4753 Response regulator containing CheY-like rece... 63 2e-09 >BH0483 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 769 Score = 86.3 bits (212), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 107/465 (23%), Positives = 210/465 (45%), Gaps = 42/465 (9%) Query: 305 SKRVYSPIQEL---ISRVMRQHQAEKPGLGYSTNELD----YLSNVFIDQRNQINELTEQ 357 S+R+Y P+QEL I+ A+KP +E + ++ NV +D N++ E Sbjct: 322 SRRIYRPVQELYQMITATFSAGSAKKP----PKDEFELIHAHIHNV-LDLNNELEE---- 372 Query: 358 WRLNKFLGRERFLRDF----LGETYHSAEEIGSQFLEWGIDLPKD--QLSVAIFRIDHFS 411 K G+ + L+ F L + + S EI +++ + PKD QL V +ID Sbjct: 373 ----KLQGQIKQLKQFFVLNLIKGHMSPSEIETKYETF--QYPKDWRQLHVLTLQIDSLE 426 Query: 412 EFSETYPEKDRRLLRFAMSNIIQESLQSSKHKLQTVDMGDDHVAVVLSAPLSEEYAK--- 468 Y ++++ +L F+++N++ E+L +L T + ++ V ++L + +E + Sbjct: 427 --GTKYAKEEKDVLLFSINNLV-ETLIPEVERL-TPALLNNTVVLILLTNVDQEQKRDGF 482 Query: 469 ---KLQVSQQLIEQYLSVGTTAAWGRILPSLTDMHEIYMETYELTQERFRFGHRALIVKA 525 K + + Q L + + +T E Y E E + R + G +++I + Sbjct: 483 VREKAEAIMNEVRQQLDISVSIGVSLPYDDITKTQEAYDEGIEALKYRIKLGCQSVIFFS 542 Query: 526 WLPDAPGELYHLPVNKERQIAQALPKGNADTVLEVIRSAVGKL--RGMPYFECKMSLITL 583 L + P +E ++ AL G+ V + + + + + + + + ++SL+ L Sbjct: 543 NLAHTHSFHTYYPKQQENELFDALKLGDKSNVKDHLDALIDGIFQKDLHPSQYQISLLRL 602 Query: 584 FMDIRRLMQEHSGQPLPSSWGLTSIEKQITQQETMENVVPWIEALVTKTLEDIVAARSLS 643 + +L+Q G + ++ Q+ +T++ V W + VA R S Sbjct: 603 LNNTIQLLQT-LGIDSVDVYDKKTLFDQLFSLQTIDEVKQWFFTKLIAPSVHTVAERMDS 661 Query: 644 KNVALIEQADRFIEEQLTDPNLSAKMLADHLGLSVNYFRSLYKAETNQSITDKISEKRLN 703 + + +Q I+ + D L+ + +A+ L + NY S+++ ETN S ++ +S RL Sbjct: 662 QYRTISDQIIHIIQNEF-DMELTLESIAERLHYNPNYLSSIFRKETNISFSEYLSSYRLA 720 Query: 704 FICQELLASDSPIEPIVQNSGFSSLNTFYSIFKKMYGMTPAQYRK 748 + LL + PI I + + + F FKK GMTP +YR+ Sbjct: 721 MAKKWLLETSMPIREIAERLCYGNSQNFIRSFKKKEGMTPGKYRQ 765 >BS_yesS [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 761 Score = 77.0 bits (188), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 132/696 (18%), Positives = 275/696 (39%), Gaps = 31/696 (4%) Query: 65 NDSTLKVYALSDQYSDFE-TFEVWSRLMDIKNANPAIDSVYLINDYTNTVVDSRLGLNDA 123 + S + A D DF ++++ +L +++ P I+ + + +V S + Sbjct: 85 DSSLMNGLAERDLEKDFTFSYQLTKKLFLLRDQQPLIEQASIFLNSPRPLVLS----PEY 140 Query: 124 EAFYDQDIIKRLRNPVTANHSVLIPRTLSLPLTGNKPKEVTTIIRFYEKGSSISAFVMNV 183 A +Q+ +++ R+ + ++HS+ R+ GN+ + I EK A ++ V Sbjct: 141 SALTEQEALRKYRSLLASDHSIYWKRS------GNQAMLIQLIPGAAEK--PFGAIMLAV 192 Query: 184 DTDNLMTLLQNNSNYANRSVTVLNDRDETVYSSTPLNQEQ--IAELRNHGSKGASGWKLF 241 D + ++LQ+ S Y + S +L+ E ++ + E+ + E++ ++ F Sbjct: 193 DPKEMESILQSLSPYPDGSALLLDQNREVLFHEGEKDFEKTLLHEIKKQPAENGH----F 248 Query: 242 QPRTQGEQLMVYASASIKGIQDWTFIETIPKSVILGKITVLRNXXXXXXXXXXXXXXXXI 301 Q +G+ V + Q WTF+ P S I + L Sbjct: 249 QMEWKGKVYSVSFGEMNRMHQQWTFVSAAPLSAITAPMVFLSKVIIVMLVICIGLAVCMT 308 Query: 302 VLISKRVYSPIQELISRVMRQHQAEKPGLGYSTNELDYLSNVFIDQRNQINELTEQWRLN 361 SK++Y PIQ L+ + +K +E ++ + D + +L +Q Sbjct: 309 WYASKKIYRPIQHLLG--LFTGGEKKTWQASGQDEFKWIEKRWQDLSLESRKLQQQLLRQ 366 Query: 362 KFLGRERFLRDFLGETYHSAEEIGSQFL--EWGIDLPKDQLSVAIFRIDHFSEFSETYPE 419 ++ FL+ L ++ E +F E G ++ + V ++ + E Sbjct: 367 TPHMKKSFLQHLLQSDFYYDNEESLRFRMEEAGWNIGGNVFHVLDLQVTGLRCEKSIFRE 426 Query: 420 KDRRLLRFAMSNIIQESLQSSKHKLQTVDMGDDHVAVVLSAPLSEEYAKKLQVSQQLIEQ 479 D +L F ++NI +E +L +D+G V V++ S + + + Sbjct: 427 GDEQLAVFTLTNIAEELAAKRVFQLSILDIGRLSVTVLVMKTNSSDLKAYITELARQFGD 486 Query: 480 YLSVGTTAAWGRILPSLTDMHEIYMETYELTQERFRFGHRALIV--KAWLPDAPGELYHL 537 + T+ +T++ ++ + + + R +F +R ++ +A P + Sbjct: 487 VTGLCLTSTLSSKTERVTEIPSLFQDV-KCGKSRRKFANRDQVIDLQADFPRDEQFAPYY 545 Query: 538 PVNKERQIAQALPKGNADTVLEVIRSAVGKLRGMPYFE--CKMSLITLFMDIRRLMQEHS 595 P E+QI Q + E+I + +L + +LI LF I+ + HS Sbjct: 546 PFELEKQIVQTIRLERKQEAKELIDGCMKELSEKAAIDRHVHSALIQLFSRIQEDIL-HS 604 Query: 596 GQPLPSSWGLTSIEKQITQQETMENVVPWIEALVTKTLEDIVAARSLSKNVALIEQADRF 655 G + + I++ W+ +V + R + L E+ Sbjct: 605 GLNPSELFQHRNPFLDISELREPNEAAAWLMDVVVTPYLSKLEGRKNRQQKQLAERVIAM 664 Query: 656 IEEQLTDPNLSAKMLADHLGLSVNYFRSLYKAETNQSITDKISEKRLNFICQELLASDSP 715 I EQ ++S + AD LG++ +K T + D +++ R+ + L+ ++ Sbjct: 665 IHEQYM-ADISLESCADALGMNSYTLSKAFKQVTGINFIDYVTQIRIEKAKELLVNTNKK 723 Query: 716 IEPIVQNSGFSSLNTFYSIFKKMYGMTPAQYRKKHR 751 I + + G+ N F IFKK GM P +R+ ++ Sbjct: 724 IHDVSEEVGYRH-NYFNRIFKKQVGMPPGVFRQMYQ 758 >BH1123 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 526 Score = 68.9 bits (167), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 102/420 (24%), Positives = 178/420 (42%), Gaps = 52/420 (12%) Query: 347 QRNQINELTEQWRLNKFLGRERFLRDFLGETYHSAEEIGSQFLEWGIDLPKDQLSVAIFR 406 Q+ + ELT + + + L +++ L + E I +FL I KDQ +A Sbjct: 129 QQGYLQELTNKVKRYEPLVETEWMKSLLTGRLRNGE-IPEEFLA-KITKWKDQQHIACSL 186 Query: 407 IDHFSEFSETYPEKDRRLLRFAMSNIIQESLQSSKHKLQTVDMGDDHVAV---------- 456 + E P D L R+++ +I+E L + ++M A+ Sbjct: 187 TYDGPQNKELKP-ADWYLFRYSVHKMIREVLNGYPIDFRYLEMDAYRSALCLHVNKSVSE 245 Query: 457 -VLSAPLSEEYAKKLQVSQQLIEQYLSVGTTAAWGRILPSLTDMHEIYMETYELTQERFR 515 VLS L E K QV Q + + +V TT +GR+ TD+ E + E + + F Sbjct: 246 PVLSCMLME---IKDQVEQGIYSSF-NVKTTVHYGRVKEDWTDLSESFHEAC-MESQSFP 300 Query: 516 FGHRALIVKAWLPDAPGELYHLPVNKERQIAQALPKGNADTVLEVIRSAVGKLRGMPY-- 573 G+ P G P+ +++ A+ +A EV+ AVG L +PY Sbjct: 301 LGN---------PYEDGTRATFPMESSQKLYDAIRYADATRAKEVLEEAVGVLGEVPYST 351 Query: 574 ---FECKMSLITL----FMDIRRLMQEHSGQPLPSSWGLTSIEKQITQQETMENVVPWIE 626 ++ + + T+ DI L+QE + + LT KQ QE WI Sbjct: 352 LVGYKVGIEIWTIITYSLYDIGILIQEMFDEDFDLAQHLT---KQGNWQE-------WIR 401 Query: 627 ALVTKTLEDIVAARSLSKNVA---LIEQADRFIEEQLTDPNLSAKMLADHLGLSVNYFRS 683 L + +E I + +N+ LIEQ +IE+ L + N++ + +AD L +S NY Sbjct: 402 FLCEQ-IEHICYHQQWDENIKHKQLIEQVMIYIEKHLAE-NITLQDIADELYISRNYLGQ 459 Query: 684 LYKAETNQSITDKISEKRLNFICQELLASDSPIEPIVQNSGFSSLNTFYSIFKKMYGMTP 743 ++K +S + ++ R+ + + + I + + G+ + F ++FKK G TP Sbjct: 460 IFKKVVGESFKNYVTRLRMEKAKKMIQEGNFLIYEVSEKVGYVNPAYFTTMFKKYTGYTP 519 >BH3679 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 257 Score = 66.6 bits (161), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 34/100 (34%), Positives = 57/100 (57%), Gaps = 1/100 (1%) Query: 649 IEQADRFIEEQLTDPNLSAKMLADHLGLSVNYFRSLYKAETNQSITDKISEKRLNFICQE 708 I +A +I+ L D + K +A + L+ +Y +L+K +TN + ++ ++ KRL Sbjct: 156 IHEALHYIDAHL-DQKIGLKDVAKSVHLNPSYLSALFKEKTNMTFSEYLTRKRLQHAKHL 214 Query: 709 LLASDSPIEPIVQNSGFSSLNTFYSIFKKMYGMTPAQYRK 748 L+++D PI I + G+ + F IFK+ GMTP QYRK Sbjct: 215 LVSTDRPISDIAEKCGYQTAKYFIKIFKEHEGMTPTQYRK 254 >SPy1556 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 246 Score = 62.8 bits (151), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 43/119 (36%), Positives = 63/119 (52%), Gaps = 5/119 (4%) Query: 630 TKTLEDIVAARSLSKNVALIEQADRFIEEQLTDPNLSAKMLADHLGLSVNYFRSLYKAET 689 T+T++++V K V+ I A I E+L D +L+ K LA LG S NY L K E Sbjct: 129 TETIQELV--EQPQKEVSAIAMA---IHERLADSDLTLKSLAQQLGFSPNYLSVLIKKEL 183 Query: 690 NQSITDKISEKRLNFICQELLASDSPIEPIVQNSGFSSLNTFYSIFKKMYGMTPAQYRK 748 D + ++RL LL S+ I I + GF +N F FK++ G+TP+QY+K Sbjct: 184 GMPFQDYLVQERLKKAKLLLLTSNLKIYEIAEQVGFEDMNYFSQRFKQLVGVTPSQYKK 242 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.132 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 51,227,544 Number of extensions: 2109375 Number of successful extensions: 5344 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 5361 Number of HSP's successfully gapped: 25 Length of query: 759 Length of database: 38,378,431 Length adjustment: 114 Effective length of query: 645 Effective length of database: 25,230,355 Effective search space: 16273578975 Effective search space used: 16273578975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 120 (50.8 bits)