BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0124.0026.p (295 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_gtaB [M] COG1210 UDP-glucose pyrophosphorylase 417 e-116 BH3717 [M] COG1210 UDP-glucose pyrophosphorylase 411 e-115 BH3652 [M] COG1210 UDP-glucose pyrophosphorylase 400 e-111 CAC2335 [M] COG1210 UDP-glucose pyrophosphorylase 394 e-109 BS_yngB [M] COG1210 UDP-glucose pyrophosphorylase 342 6e-94 >BS_gtaB [M] COG1210 UDP-glucose pyrophosphorylase Length = 292 Score = 417 bits (1071), Expect = e-116, Method: Compositional matrix adjust. Identities = 200/290 (68%), Positives = 241/290 (83%) Query: 2 KIRKAIIPAAGLGTRFLPATKAMPKEMLPIVDKPTIQYIVEEAVASGIEDIIIVTGKGKR 61 K+RKAIIPAAGLGTRFLPATKAMPKEMLPIVDKPTIQYI+EEAV +GIEDIIIVTGK KR Sbjct: 3 KVRKAIIPAAGLGTRFLPATKAMPKEMLPIVDKPTIQYIIEEAVEAGIEDIIIVTGKSKR 62 Query: 62 AIEDHFDNSFELEFNLAEKQKWELLESVRKSSEMADIHYIRQKEPRGLGHAIWCARKFIG 121 AIEDHFD S ELE NL EK K ELLE V+K+S +ADIHYIRQKEP+GLGHA+WCAR FIG Sbjct: 63 AIEDHFDYSPELERNLEEKGKTELLEKVKKASNLADIHYIRQKEPKGLGHAVWCARNFIG 122 Query: 122 NEPFAVLLGDDIVQADKPCLRQMIEVYEQYKSSIVGVQPVPWEDVSRYGLVDGTELAERV 181 +EPFAVLLGDDIVQA+ P LRQ+++ YE+ SSI+GVQ VP E+ RYG++D R Sbjct: 123 DEPFAVLLGDDIVQAETPGLRQLMDEYEKTLSSIIGVQQVPEEETHRYGIIDPLTSEGRR 182 Query: 182 YKANRLVEKPKREEAPSNLAILGRYILTPRIFDMLEEQQVGVGGEIQLTDAISRLSEVER 241 Y+ VEKP + APSNLAILGRY+ TP IF LEEQQVG GGEIQLTDAI +L+E++R Sbjct: 183 YQVKNFVEKPPKGTAPSNLAILGRYVFTPEIFMYLEEQQVGAGGEIQLTDAIQKLNEIQR 242 Query: 242 IIAYDFEGKRHDVGEKMGFIQTTIHYALQHEELKEGLLDYLKEVINSEAL 291 + AYDFEGKR+DVGEK+GFI TT+ +A+Q +EL++ L+ +++ ++N E + Sbjct: 243 VFAYDFEGKRYDVGEKLGFITTTLEFAMQDKELRDQLVPFMEGLLNKEEI 292 >BH3717 [M] COG1210 UDP-glucose pyrophosphorylase Length = 293 Score = 411 bits (1056), Expect = e-115, Method: Compositional matrix adjust. Identities = 195/285 (68%), Positives = 238/285 (83%), Gaps = 1/285 (0%) Query: 2 KIRKAIIPAAGLGTRFLPATKAMPKEMLPIVDKPTIQYIVEEAVASGIEDIIIVTGKGKR 61 KIRKAIIPAAGLGTRFLPATKAMPKEMLPIVDKPTIQYIVEEA++SGIEDIIIVTGKGKR Sbjct: 3 KIRKAIIPAAGLGTRFLPATKAMPKEMLPIVDKPTIQYIVEEAISSGIEDIIIVTGKGKR 62 Query: 62 AIEDHFDNSFELEFNLAEKQKWELLESVRKSSEMADIHYIRQKEPRGLGHAIWCARKFIG 121 AIEDHFD+SFELE LAEK+K++LLE V +S+++ DIHYIRQKEP+GLGHA+WCARKFIG Sbjct: 63 AIEDHFDHSFELEQTLAEKEKYDLLEKVNESTKV-DIHYIRQKEPKGLGHAVWCARKFIG 121 Query: 122 NEPFAVLLGDDIVQADKPCLRQMIEVYEQYKSSIVGVQPVPWEDVSRYGLVDGTELAERV 181 +EPFAVLLGDDIVQA+ PCL+Q+I+ YEQ +SS++GVQ V + RYG+VD + R Sbjct: 122 DEPFAVLLGDDIVQAETPCLKQLIDQYEQTESSVIGVQTVADHETHRYGIVDPIDQKGRT 181 Query: 182 YKANRLVEKPKREEAPSNLAILGRYILTPRIFDMLEEQQVGVGGEIQLTDAISRLSEVER 241 Y + VEKPK APSNLAI+GRY+LTP IF L + G GGEIQLTDAI RL+E++ Sbjct: 182 YTVKQFVEKPKPGTAPSNLAIMGRYVLTPEIFTFLGRHETGAGGEIQLTDAIERLNEIQH 241 Query: 242 IIAYDFEGKRHDVGEKMGFIQTTIHYALQHEELKEGLLDYLKEVI 286 + AYDFEGKR+DVGEK+GF++TT+ +AL E +K+ +L+ ++ Sbjct: 242 VYAYDFEGKRYDVGEKIGFVKTTVEFALHDEAIKDEFARFLEAIL 286 >BH3652 [M] COG1210 UDP-glucose pyrophosphorylase Length = 297 Score = 400 bits (1028), Expect = e-111, Method: Compositional matrix adjust. Identities = 193/287 (67%), Positives = 233/287 (81%), Gaps = 2/287 (0%) Query: 1 MKIRKAIIPAAGLGTRFLPATKAMPKEMLPIVDKPTIQYIVEEAVASGIEDIIIVTGKGK 60 M ++KAIIPAAGLGTRFLPATKA PKEMLPIVDKPTIQYI+EEA+ SGIEDII+VTG+GK Sbjct: 1 MHVKKAIIPAAGLGTRFLPATKAQPKEMLPIVDKPTIQYIIEEAIESGIEDIIVVTGRGK 60 Query: 61 RAIEDHFDNSFELEFNLAEKQKWELLESVRKSSEMADIHYIRQKEPRGLGHAIWCARKFI 120 RAIEDHFD SFELE L +K K +LLE V+ S M +IHYIRQKE GLGHAIWCARKFI Sbjct: 61 RAIEDHFDKSFELEETLEKKNKSKLLEEVKAISSMTNIHYIRQKEANGLGHAIWCARKFI 120 Query: 121 GNEPFAVLLGDDIVQADKPCLRQMIEVYEQYKSSIVGVQPVPWEDVSRYGLVD--GTELA 178 GNEPFAVLLGDDIVQ+D PCL+Q+I+V+++Y SS+VGVQ VP EDVS+YG+V G+E+ Sbjct: 121 GNEPFAVLLGDDIVQSDVPCLKQLIDVFDRYHSSVVGVQEVPNEDVSKYGIVSPKGSEIE 180 Query: 179 ERVYKANRLVEKPKREEAPSNLAILGRYILTPRIFDMLEEQQVGVGGEIQLTDAISRLSE 238 V A VEKPK EEAPSN AI+GRY+L P IFD+LE G GGEIQLTDAI L+E Sbjct: 181 ANVIHAAGFVEKPKLEEAPSNYAIMGRYVLRPEIFDILENLPPGAGGEIQLTDAIKVLNE 240 Query: 239 VERIIAYDFEGKRHDVGEKMGFIQTTIHYALQHEELKEGLLDYLKEV 285 + ++AY+F+G R+DVG+K GFI+ TI ALQ ++L E + Y+K++ Sbjct: 241 SQVVLAYNFDGVRYDVGDKFGFIKATIDLALQRDDLSEDVAQYIKDI 287 >CAC2335 [M] COG1210 UDP-glucose pyrophosphorylase Length = 303 Score = 394 bits (1012), Expect = e-109, Method: Compositional matrix adjust. Identities = 185/293 (63%), Positives = 235/293 (80%) Query: 1 MKIRKAIIPAAGLGTRFLPATKAMPKEMLPIVDKPTIQYIVEEAVASGIEDIIIVTGKGK 60 M+++KAIIPAAGLGTRFLPATKA PKEMLPIVDKPTIQYI+EEAV SGIE+I+++TG+ K Sbjct: 1 MRVKKAIIPAAGLGTRFLPATKAQPKEMLPIVDKPTIQYIIEEAVQSGIEEILVITGRNK 60 Query: 61 RAIEDHFDNSFELEFNLAEKQKWELLESVRKSSEMADIHYIRQKEPRGLGHAIWCARKFI 120 RAIEDHFD SFELE L K ELL+ V+ S +A+I+YIRQKEPRGLGHAI CAR F+ Sbjct: 61 RAIEDHFDKSFELESELESHNKKELLKVVKDISNLANIYYIRQKEPRGLGHAINCARTFV 120 Query: 121 GNEPFAVLLGDDIVQADKPCLRQMIEVYEQYKSSIVGVQPVPWEDVSRYGLVDGTELAER 180 GNEPFAVLLGDD+V A PCL+Q+I Y +YK+SI+GVQ VP +VS+YG+V+G + + Sbjct: 121 GNEPFAVLLGDDVVNAKVPCLKQLINCYNEYKTSILGVQRVPNSEVSKYGIVNGMHIEDG 180 Query: 181 VYKANRLVEKPKREEAPSNLAILGRYILTPRIFDMLEEQQVGVGGEIQLTDAISRLSEVE 240 VYK LVEKPK+EEAPS+LAILGRYI+TP IFD+L+ + G G EIQLTDA+ L + E Sbjct: 181 VYKVKDLVEKPKQEEAPSDLAILGRYIITPSIFDILDNTKPGKGDEIQLTDALKTLMKTE 240 Query: 241 RIIAYDFEGKRHDVGEKMGFIQTTIHYALQHEELKEGLLDYLKEVINSEALKA 293 + AY F G+R+DVG+K+GF+Q T+ YAL+ EELK+ ++YL + ++ K+ Sbjct: 241 AMYAYVFSGRRYDVGDKLGFLQATVEYALKREELKKPFMNYLFTIKDNPNFKS 293 >BS_yngB [M] COG1210 UDP-glucose pyrophosphorylase Length = 297 Score = 342 bits (876), Expect = 6e-94, Method: Compositional matrix adjust. Identities = 167/294 (56%), Positives = 217/294 (73%) Query: 2 KIRKAIIPAAGLGTRFLPATKAMPKEMLPIVDKPTIQYIVEEAVASGIEDIIIVTGKGKR 61 K+RKA+IPAAGLGTRFLPATKA PKEMLPIVDKP IQYIVEEA SGIEDI+I+TG+ KR Sbjct: 4 KVRKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPAIQYIVEEAAESGIEDILIITGRNKR 63 Query: 62 AIEDHFDNSFELEFNLAEKQKWELLESVRKSSEMADIHYIRQKEPRGLGHAIWCARKFIG 121 +IEDHFD S ELEFNL EK K E L+ +++ +++A+IHYIRQKEP GLGHA+ CA FIG Sbjct: 64 SIEDHFDRSAELEFNLREKGKTETLKEMQQIADLANIHYIRQKEPLGLGHAVLCAEHFIG 123 Query: 122 NEPFAVLLGDDIVQADKPCLRQMIEVYEQYKSSIVGVQPVPWEDVSRYGLVDGTELAERV 181 +EPFAVLLGDDI+ ++ P LRQ+++VY+ Y + +VGVQ V EDVS+YG+++ + V Sbjct: 124 DEPFAVLLGDDIMVSETPALRQLMDVYDVYGTEVVGVQSVLPEDVSKYGIINTSGSQGHV 183 Query: 182 YKANRLVEKPKREEAPSNLAILGRYILTPRIFDMLEEQQVGVGGEIQLTDAISRLSEVER 241 Y+ N LVEKP EEAPS +A++GRY+L IF +L+ G G EIQLTDA+ + E Sbjct: 184 YEVNDLVEKPSPEEAPSEIAVMGRYVLNSSIFSVLKTIGRGAGNEIQLTDALREVCRKEP 243 Query: 242 IIAYDFEGKRHDVGEKMGFIQTTIHYALQHEELKEGLLDYLKEVINSEALKAAR 295 I A EG R+D+G+K+G + + L E++ LL YL++VI E + R Sbjct: 244 IHARLLEGNRYDIGDKLGCFKASTEIGLMRPEMRSQLLAYLEDVIKRETKEMLR 297 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,958,253 Number of extensions: 926240 Number of successful extensions: 6200 Number of sequences better than 1.0e-05: 132 Number of HSP's gapped: 6068 Number of HSP's successfully gapped: 132 Length of query: 295 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 189 Effective length of database: 26,153,027 Effective search space: 4942922103 Effective search space used: 4942922103 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)