BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0124.0023.p (187 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MTH1790 [M] COG1898 dTDP-4-dehydrorhamnose 3,5-epimerase and rel... 201 5e-52 mlr7551 [M] COG1898 dTDP-4-dehydrorhamnose 3,5-epimerase and rel... 193 1e-49 SMb21325 [M] COG1898 dTDP-4-dehydrorhamnose 3,5-epimerase and re... 193 1e-49 MA3780 [M] COG1898 dTDP-4-dehydrorhamnose 3,5-epimerase and rela... 186 1e-47 alr4489 [M] COG1898 dTDP-4-dehydrorhamnose 3,5-epimerase and rel... 182 4e-46 >MTH1790 [M] COG1898 dTDP-4-dehydrorhamnose 3,5-epimerase and related enzymes Length = 268 Score = 201 bits (511), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 12/180 (6%), Positives = 35/180 (19%), Gaps = 4/180 (2%) Query: 3 KMKFTKTNLEGVLVVEPALFGDHRGWFMETYSEAKFQEQNLAYQFVQDNQSYSAVKGTLR 62 ++ + + + L + + + F + + + + Sbjct: 3 RVDLSIFIPDSLTAETGDLKIKTYKVVLIARAASIFGVKRIVIYHDDADGEAR-FIRDIL 61 Query: 63 GLHFQLNPKAQTKLARCTRGSIYDVAVDIRQGSPSYGKWFGIELTAENKKQLLIPKGFAH 122 + K+ R + + + + + KG Sbjct: 62 TYMDT-PQYLRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKGTLV 120 Query: 123 GFMTLTEEVEVQYKCDELYAPECDGSILWNDPDIGIEWPIDVTP--VLSAKDENAPLLKD 180 + + + E VL + A LK Sbjct: 121 DIGADKLALCREKLTVNRIMSFRVVRLGKEILIEPDEPEDRYWGYEVLDTRRNLAESLKT 180 >mlr7551 [M] COG1898 dTDP-4-dehydrorhamnose 3,5-epimerase and related enzymes Length = 484 Score = 193 bits (491), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 9/181 (4%), Positives = 30/181 (16%), Gaps = 1/181 (0%) Query: 4 MKFTKTNLEGVLVVEPALFGDHRGWFMETYSEAKFQEQNLAYQFVQDNQSYSAVKGTLRG 63 M + L ++ + + + Q Sbjct: 1 MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDENARKQGLFVYPSLTVT 60 Query: 64 LHFQLNPKAQTKLARCTRGSIYDVAVDIRQGSPSYGKWFGIELTAENKKQLLIPKGFAHG 123 +T+ + R + L ++ + + H Sbjct: 61 WDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLTLLYQDYGAHISVQPSQHE 120 Query: 124 FMTLTEEVEVQYKCDELYAPECDGSILWNDPDIGIEWP-IDVTPVLSAKDENAPLLKDVS 182 + D + + + + + + V Sbjct: 121 IPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETADGIYHPTEFSPLSHFDARRVD 180 Query: 183 L 183 Sbjct: 181 F 181 >SMb21325 [M] COG1898 dTDP-4-dehydrorhamnose 3,5-epimerase and related enzymes Length = 187 Score = 193 bits (491), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 94/181 (51%), Positives = 119/181 (65%), Gaps = 1/181 (0%) Query: 4 MKFTKTNLEGVLVVEPALFGDHRGWFMETYSEAKFQEQNLAYQFVQDNQSYSAVKGTLRG 63 ++ L+GVL + P GD RG+F ETY+ +A FVQDN SYSA GTLRG Sbjct: 2 LEVRSLGLDGVLEIVPRRLGDERGFFSETYNAETLAAHGVAAIFVQDNHSYSAAAGTLRG 61 Query: 64 LHFQLNPKAQTKLARCTRGSIYDVAVDIRQGSPSYGKWFGIELTAENKKQLLIPKGFAHG 123 LH+QL PKAQ KL R RG ++DV VDIR+GSP++ KW +EL+AE Q+L+P GFAHG Sbjct: 62 LHYQLPPKAQAKLVRVVRGRVFDVVVDIRKGSPTFAKWVALELSAEKWNQILVPVGFAHG 121 Query: 124 FMTLTEEVEVQYKCDELYAPECDGSILWNDPDIGIEWPIDVTPV-LSAKDENAPLLKDVS 182 +TL E EV YK E Y+ E + SI ++DP+IGI WP + LSAKD NAPLL + Sbjct: 122 LVTLDPETEVLYKVSEHYSAEHERSIRYDDPEIGIGWPAATDRLQLSAKDRNAPLLHESI 181 Query: 183 L 183 L Sbjct: 182 L 182 >MA3780 [M] COG1898 dTDP-4-dehydrorhamnose 3,5-epimerase and related enzymes Length = 183 Score = 186 bits (473), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 88/184 (47%), Positives = 122/184 (66%), Gaps = 3/184 (1%) Query: 4 MKFTKTNLEGVLVVEPALFGDHRGWFMETYSEAKFQEQ-NLAYQFVQDNQSYSAVKGTLR 62 MK T ++ + ++EP +FGD RG+F E+Y++ K Y FVQDN+S S+ G +R Sbjct: 1 MKLINTEIKDLFILEPDVFGDDRGYFFESYNKMKLDALIGKMYHFVQDNESKSSY-GVIR 59 Query: 63 GLHFQLNPKAQTKLARCTRGSIYDVAVDIRQGSPSYGKWFGIELTAENKKQLLIPKGFAH 122 GLH+QL P +QTKL R +G +YDVAVD+R+ SP++GKWFG+EL+ ENK+Q +IPKGFAH Sbjct: 60 GLHYQLAPFSQTKLVRVLQGKVYDVAVDLRKDSPTFGKWFGVELSGENKRQFIIPKGFAH 119 Query: 123 GFMTLTEEVEVQYKCDELYAPECDGSILWNDPDIGIEWPI-DVTPVLSAKDENAPLLKDV 181 GF L+E YKCDE Y PE + I +NDP + I+W + D +S KD P + Sbjct: 120 GFSVLSETAVFVYKCDEYYHPETEAGIAYNDPSLNIDWKVRDEDIKVSPKDGLLPRFEKA 179 Query: 182 SLNF 185 +NF Sbjct: 180 EINF 183 >alr4489 [M] COG1898 dTDP-4-dehydrorhamnose 3,5-epimerase and related enzymes Length = 359 Score = 182 bits (461), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 13/181 (7%), Positives = 31/181 (17%), Gaps = 3/181 (1%) Query: 4 MKFTKTNLEGVLVVEPALFGDHRGWFMETYSEAKFQEQ-NLAYQFVQDNQSYSAVKGTLR 62 M+ + + + + K + + + A G R Sbjct: 1 MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADA-FGVAR 59 Query: 63 GLHFQLNPKAQTKLARCTRGSIYDVAVDIRQGSPSYGKWFGIELTAENKKQLLIPKGFAH 122 +A + A D+ S + + Sbjct: 60 LEEALRL-RAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVT 118 Query: 123 GFMTLTEEVEVQYKCDELYAPECDGSILWNDPDIGIEWPIDVTPVLSAKDENAPLLKDVS 182 +M L + E + + K + Sbjct: 119 VWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIF 178 Query: 183 L 183 Sbjct: 179 N 179 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,675,798 Number of extensions: 502723 Number of successful extensions: 728 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 715 Number of HSP's successfully gapped: 16 Length of query: 187 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 87 Effective length of database: 26,845,031 Effective search space: 2335517697 Effective search space used: 2335517697 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 112 (47.8 bits)